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The map label for this gene is recF

Identifier: 222527396

GI number: 222527396

Start: 5189830

End: 5191008

Strand: Reverse

Name: recF

Synonym: Chy400_4187

Alternate gene names: 222527396

Gene position: 5191008-5189830 (Counterclockwise)

Preceding gene: 222527397

Following gene: 222527393

Centisome position: 98.52

GC content: 59.37

Gene sequence:

>1179_bases
ATGTATGTTCACCATCTGTTTCTGCGCGATTTCCGTAACTACCGACGCCTTGATCTCGCGCTAGCACCGGCAACCACACT
CTTTTACGGGCCAAATGCCGCCGGCAAAACGAGTTTGCTTGAAGCAATCTTCTACCTCGCGACAACGCGCTCGCCACGCC
TGAGTAGTGATCGTGAACTGGTGCGCTGGGATGCAGTGGGAGAGGCGGGTACCCCGCCGTTTGCCCGTATCGCTGCCGAT
GTAGAGCGGCGGATCGGGCCGGTGCGCCTGGAGGTATTGGTACAACGACGGGCAGACGATGATGGGCAGCCACTGAACGG
TGCGCAAAAGCTGGTGCGCATTGATAAGCGTCCGGCGCGGGCAATCGATCTGATAGGTCAGTTGCGGGTGGTGCTCTTTA
CCCCTACCGATCTGACGCTGGTTGATGGGCCACCGGCAGAACGGCGACGTTACCTCGATATCACGCTCTCGCAGCTCGAT
CCGCATTACGTGCGCACATTAGCGCATTATCAGAAGATACTCTTGCAACGAAACAGTTTGTTACGCGCCTGGCGCGAGCA
GCGACGAGTGCCACGCCACGTTGATGCCGAGCTGGCCTATTGGGATCAGGAGCTAGCGGCGGCTGGCGGGTATCTGCTGG
CCGAACGGTTACGCGCAATTGTTGAACTCAATGATCTGGCCGGCCCGCTCTATCAGGAGATGAGCGGTGGCGAAGATCGG
TTGCAGATCGAGTACGCAGCCAGTTGTGACCTGGGAACTGCAAGAGATGCCGGCGGCCTGGCCGAGCGCTTGCTGCTGGC
GTTTGCAGCCCAACGCAGTGATGAACTGGCCCGTGGTCAGACCCTCTGCGGCCCTCATCGCGACGATCTGATCTTCACGG
TGGCCGGGATCAATCTCGGTCGCTACGGTTCCCGTGGTCAGCAGCGTTCGATTGCGCTGGCGTTGAAGATTGGTGAGGCC
GGTTTGATGCGACGGCGCAGCGGTGAAGCACCGGTGCTGCTGCTCGATGATGTATTGAGTGAGCTTGATGCTCAGCGCCG
CGCCCATCTCCTTGCCTTGATCCATCATCCCGATCAGCAGACGCTGCTTACCGCTACCGATCTCAGCGATTTCAGTGCCG
ATTTCCTGGCTGCTGTCCGTCGCTATCGGGTTGAAGATGGTCAGGTGTTTGCCGGCTGA

Upstream 100 bases:

>100_bases
AACTGCGGGTGGCTATGTTTTGTAGCGGCTGTGCCAATCTTCAGGCGCTGCGGAACCTGCGCCTGGGTGAGCGCTCGTAG
CCTGCGGCGATGGCCGATAT

Downstream 100 bases:

>100_bases
AGTCGGCGGTAGATAAATGTAAGTTGACGCAATCTGTCGGCCAGTTCTGCGCTGAGCGCTTCAGCCGAAAAGCGCCATGC
CCAGTTACCACCCAATCGAC

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MYVHHLFLRDFRNYRRLDLALAPATTLFYGPNAAGKTSLLEAIFYLATTRSPRLSSDRELVRWDAVGEAGTPPFARIAAD
VERRIGPVRLEVLVQRRADDDGQPLNGAQKLVRIDKRPARAIDLIGQLRVVLFTPTDLTLVDGPPAERRRYLDITLSQLD
PHYVRTLAHYQKILLQRNSLLRAWREQRRVPRHVDAELAYWDQELAAAGGYLLAERLRAIVELNDLAGPLYQEMSGGEDR
LQIEYAASCDLGTARDAGGLAERLLLAFAAQRSDELARGQTLCGPHRDDLIFTVAGINLGRYGSRGQQRSIALALKIGEA
GLMRRRSGEAPVLLLDDVLSELDAQRRAHLLALIHHPDQQTLLTATDLSDFSADFLAAVRRYRVEDGQVFAG

Sequences:

>Translated_392_residues
MYVHHLFLRDFRNYRRLDLALAPATTLFYGPNAAGKTSLLEAIFYLATTRSPRLSSDRELVRWDAVGEAGTPPFARIAAD
VERRIGPVRLEVLVQRRADDDGQPLNGAQKLVRIDKRPARAIDLIGQLRVVLFTPTDLTLVDGPPAERRRYLDITLSQLD
PHYVRTLAHYQKILLQRNSLLRAWREQRRVPRHVDAELAYWDQELAAAGGYLLAERLRAIVELNDLAGPLYQEMSGGEDR
LQIEYAASCDLGTARDAGGLAERLLLAFAAQRSDELARGQTLCGPHRDDLIFTVAGINLGRYGSRGQQRSIALALKIGEA
GLMRRRSGEAPVLLLDDVLSELDAQRRAHLLALIHHPDQQTLLTATDLSDFSADFLAAVRRYRVEDGQVFAG
>Mature_392_residues
MYVHHLFLRDFRNYRRLDLALAPATTLFYGPNAAGKTSLLEAIFYLATTRSPRLSSDRELVRWDAVGEAGTPPFARIAAD
VERRIGPVRLEVLVQRRADDDGQPLNGAQKLVRIDKRPARAIDLIGQLRVVLFTPTDLTLVDGPPAERRRYLDITLSQLD
PHYVRTLAHYQKILLQRNSLLRAWREQRRVPRHVDAELAYWDQELAAAGGYLLAERLRAIVELNDLAGPLYQEMSGGEDR
LQIEYAASCDLGTARDAGGLAERLLLAFAAQRSDELARGQTLCGPHRDDLIFTVAGINLGRYGSRGQQRSIALALKIGEA
GLMRRRSGEAPVLLLDDVLSELDAQRRAHLLALIHHPDQQTLLTATDLSDFSADFLAAVRRYRVEDGQVFAG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=416, Percent_Identity=26.9230769230769, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CHLAA (A9WDD4)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001637442.1
- ProteinModelPortal:   A9WDD4
- SMR:   A9WDD4
- GeneID:   5825686
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3876
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK974188
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- SMART:   SM00382
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 43841; Mature: 43841

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVHHLFLRDFRNYRRLDLALAPATTLFYGPNAAGKTSLLEAIFYLATTRSPRLSSDREL
CCCHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
VRWDAVGEAGTPPFARIAADVERRIGPVRLEVLVQRRADDDGQPLNGAQKLVRIDKRPAR
EEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHH
AIDLIGQLRVVLFTPTDLTLVDGPPAERRRYLDITLSQLDPHYVRTLAHYQKILLQRNSL
HHHHHHCEEEEEECCCCEEEECCCCHHHCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHH
LRAWREQRRVPRHVDAELAYWDQELAAAGGYLLAERLRAIVELNDLAGPLYQEMSGGEDR
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCE
LQIEYAASCDLGTARDAGGLAERLLLAFAAQRSDELARGQTLCGPHRDDLIFTVAGINLG
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCC
RYGSRGQQRSIALALKIGEAGLMRRRSGEAPVLLLDDVLSELDAQRRAHLLALIHHPDQQ
CCCCCCCCCEEEEEEEECCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEEHEEECCCCC
TLLTATDLSDFSADFLAAVRRYRVEDGQVFAG
EEEEECCCCHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MYVHHLFLRDFRNYRRLDLALAPATTLFYGPNAAGKTSLLEAIFYLATTRSPRLSSDREL
CCCHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
VRWDAVGEAGTPPFARIAADVERRIGPVRLEVLVQRRADDDGQPLNGAQKLVRIDKRPAR
EEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHH
AIDLIGQLRVVLFTPTDLTLVDGPPAERRRYLDITLSQLDPHYVRTLAHYQKILLQRNSL
HHHHHHCEEEEEECCCCEEEECCCCHHHCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHH
LRAWREQRRVPRHVDAELAYWDQELAAAGGYLLAERLRAIVELNDLAGPLYQEMSGGEDR
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCE
LQIEYAASCDLGTARDAGGLAERLLLAFAAQRSDELARGQTLCGPHRDDLIFTVAGINLG
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCC
RYGSRGQQRSIALALKIGEAGLMRRRSGEAPVLLLDDVLSELDAQRRAHLLALIHHPDQQ
CCCCCCCCCEEEEEEEECCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEEHEEECCCCC
TLLTATDLSDFSADFLAAVRRYRVEDGQVFAG
EEEEECCCCHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA