Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 222527342

GI number: 222527342

Start: 5113652

End: 5115514

Strand: Direct

Name: tolB [H]

Synonym: Chy400_4128

Alternate gene names: 222527342

Gene position: 5113652-5115514 (Clockwise)

Preceding gene: 222527341

Following gene: 222527347

Centisome position: 97.05

GC content: 57.92

Gene sequence:

>1863_bases
ATGTACCGGCCAGGAATGATGTGGAGCATGCTCGCCCTGATCGGTGCGCTCATGCTCAGCAGCGTGGCCCCGCTCGCCGC
CGCACCCTTCTCCGGGCGCACCAGCTTTGCCGACCCGCGCTTTGCAGCCATCTGGAGCCGTACCGACAGCGAGGCCGTGC
GTGGTGGACGCACCTGGTATTGGGGACCAGGACCGTGGTTCGATTACGGCGAGTTCTACCGGGAAAGCCCCAATGCAATT
CGCACCGTTCAGTACTTTGACAAAGCCCGCATGGAGATCAATCGCCCTGACGACGGCATTGTAACGAACGGCCTTCTGGT
GAAAGAGCTGGTCAGCGGACGAATGCAGCTCGGTGATTTTCCCTACGATGTCACCTATCGCGATCCTTCAGACGTACCGG
TCGCCGGTAATCCGCGCGCTGCTAACACGATTGCCCCTGGCTACCGCGATTTCGCCGGTATCGCAACAATTGACAATGGC
TACCGTGATCCTTCACGACTAAACGAGCGCGTCTCCGCTGTGATCGCTCGCGGCGGCAACATTGGTATTCGCGAGGATCT
TGCCCGGCCCGAAACGACGATTGTGCAGTATAACAGCGTGACGGGTCACAACATTCCGCGTGTCTTCTGGGATTTTATGA
ACGCCCGTGGCCGCGTCGTTGAGAATGGGCGCGTCGTTACCGCACCGATTGTTGACTGGCTCTTCGCGATGGGCTATCCG
ATCACCGATCCCTACTGGACACGTGCCGTTGTTGGTGATACCGAACGCGATGTCCTGGTGCAGCTCTTCGAGCGACGGGT
CTTGACCTACACCCCGGACAATCCGCCCGGTTATCAGGTCGAGATGGGGAATGTCGGGCAGCACTACTTTCAGTGGCGCT
ATCCGCATCTGGGTGCACCGTGGGTTGCCCCCGATCCGGCCACCCCGCTGATCTACGCCTCGAATATCGACACCGGCAGC
CACTGGGAACTCTACCGCGCCACCTTTGACGGCGGTGGACGACGGTTGACCTTCAACAATGGTGAGACGGTGGCCTTCTC
GTGGCGGCGAAGCTGGGACCCTAACCAACACTTTCTGGTGGTTGACTCACGGCGGAATAGTCCTCAGTATCGCCAGATCT
ATTTGCTCAATGCTGTTGCTGCCGATGCCGGTGAGCAGGCAGCCGGCGCTAGCGTGGTTCGGATTAGCTACAGCAATGCT
GACGGTAACTTCCCGCCATCGGATAACGACTCCTCAAGCATTCCGGGGAGCGAGTACAACGCCTCGGTGTCGCCGGATGG
TACGCTGCTGGCGTTTGTCTCCGAGCGACAGGGGATTCCACAACTCTACCTTCGCCGCCTGAATGTGTCACCATTGCGCG
GCTACGCCAGTCCAATTACCGCCTACGACCGGCCATGTAATGTTGAAAGCCCGACCTGGTCGCCCGATGGTCGCTATCTG
TTCTGGGTTACGAATTGTGAGGGGAACTTTGAGATCTATCGGGCTACTATCGTGTTCTACTACATTGATGACCTCTTTGC
GAGCATAAGTCTGAACAATATTCGCAACCTGAGCAACAACGCAGCAAACGACCGCTTCGCGCGCATCTCACCTGACGGCA
GGACGATTGCCTTCGCCAGCGACCGCGACGGGAACTGGGAAATCTATGTGATGAACAGCGATGGGAGTAATGTGCGTCGA
TTGACCAACAACGCAGCAACCGACGACTCGCCAACCTGGTCACTTACCAACCAACAGCTCGCGTTTGCCAGTGATCGTGA
CGGTGACTTCGAGATTTACATTCTCAATGTCAGTGATGGCGCTATCGTGCAGCAGGTGACACAAAACACTGCCCAGGATC
GCTGGCCGTTGTGGGCGCAATAA

Upstream 100 bases:

>100_bases
TCTTCACACTATTTTGAGCTTAATATCAGATGTTATGGGAACTACAGGGATTATAATATCGTCTACAGGGTGTATCAGGC
AGTAATTCAAGGAGGATGTT

Downstream 100 bases:

>100_bases
ACCAGCCTGACAGGGGATCAGGGTGATCTAGGGTAAGTTCATCTACCCATGCTAACGGGCGTGGGGCGGGTTGGCAGCCC
ACCTCACGTCCTGATGGTAG

Product: WD40 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MYRPGMMWSMLALIGALMLSSVAPLAAAPFSGRTSFADPRFAAIWSRTDSEAVRGGRTWYWGPGPWFDYGEFYRESPNAI
RTVQYFDKARMEINRPDDGIVTNGLLVKELVSGRMQLGDFPYDVTYRDPSDVPVAGNPRAANTIAPGYRDFAGIATIDNG
YRDPSRLNERVSAVIARGGNIGIREDLARPETTIVQYNSVTGHNIPRVFWDFMNARGRVVENGRVVTAPIVDWLFAMGYP
ITDPYWTRAVVGDTERDVLVQLFERRVLTYTPDNPPGYQVEMGNVGQHYFQWRYPHLGAPWVAPDPATPLIYASNIDTGS
HWELYRATFDGGGRRLTFNNGETVAFSWRRSWDPNQHFLVVDSRRNSPQYRQIYLLNAVAADAGEQAAGASVVRISYSNA
DGNFPPSDNDSSSIPGSEYNASVSPDGTLLAFVSERQGIPQLYLRRLNVSPLRGYASPITAYDRPCNVESPTWSPDGRYL
FWVTNCEGNFEIYRATIVFYYIDDLFASISLNNIRNLSNNAANDRFARISPDGRTIAFASDRDGNWEIYVMNSDGSNVRR
LTNNAATDDSPTWSLTNQQLAFASDRDGDFEIYILNVSDGAIVQQVTQNTAQDRWPLWAQ

Sequences:

>Translated_620_residues
MYRPGMMWSMLALIGALMLSSVAPLAAAPFSGRTSFADPRFAAIWSRTDSEAVRGGRTWYWGPGPWFDYGEFYRESPNAI
RTVQYFDKARMEINRPDDGIVTNGLLVKELVSGRMQLGDFPYDVTYRDPSDVPVAGNPRAANTIAPGYRDFAGIATIDNG
YRDPSRLNERVSAVIARGGNIGIREDLARPETTIVQYNSVTGHNIPRVFWDFMNARGRVVENGRVVTAPIVDWLFAMGYP
ITDPYWTRAVVGDTERDVLVQLFERRVLTYTPDNPPGYQVEMGNVGQHYFQWRYPHLGAPWVAPDPATPLIYASNIDTGS
HWELYRATFDGGGRRLTFNNGETVAFSWRRSWDPNQHFLVVDSRRNSPQYRQIYLLNAVAADAGEQAAGASVVRISYSNA
DGNFPPSDNDSSSIPGSEYNASVSPDGTLLAFVSERQGIPQLYLRRLNVSPLRGYASPITAYDRPCNVESPTWSPDGRYL
FWVTNCEGNFEIYRATIVFYYIDDLFASISLNNIRNLSNNAANDRFARISPDGRTIAFASDRDGNWEIYVMNSDGSNVRR
LTNNAATDDSPTWSLTNQQLAFASDRDGDFEIYILNVSDGAIVQQVTQNTAQDRWPLWAQ
>Mature_620_residues
MYRPGMMWSMLALIGALMLSSVAPLAAAPFSGRTSFADPRFAAIWSRTDSEAVRGGRTWYWGPGPWFDYGEFYRESPNAI
RTVQYFDKARMEINRPDDGIVTNGLLVKELVSGRMQLGDFPYDVTYRDPSDVPVAGNPRAANTIAPGYRDFAGIATIDNG
YRDPSRLNERVSAVIARGGNIGIREDLARPETTIVQYNSVTGHNIPRVFWDFMNARGRVVENGRVVTAPIVDWLFAMGYP
ITDPYWTRAVVGDTERDVLVQLFERRVLTYTPDNPPGYQVEMGNVGQHYFQWRYPHLGAPWVAPDPATPLIYASNIDTGS
HWELYRATFDGGGRRLTFNNGETVAFSWRRSWDPNQHFLVVDSRRNSPQYRQIYLLNAVAADAGEQAAGASVVRISYSNA
DGNFPPSDNDSSSIPGSEYNASVSPDGTLLAFVSERQGIPQLYLRRLNVSPLRGYASPITAYDRPCNVESPTWSPDGRYL
FWVTNCEGNFEIYRATIVFYYIDDLFASISLNNIRNLSNNAANDRFARISPDGRTIAFASDRDGNWEIYVMNSDGSNVRR
LTNNAATDDSPTWSLTNQQLAFASDRDGDFEIYILNVSDGAIVQQVTQNTAQDRWPLWAQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=187, Percent_Identity=29.9465240641711, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 69555; Mature: 69555

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRPGMMWSMLALIGALMLSSVAPLAAAPFSGRTSFADPRFAAIWSRTDSEAVRGGRTWY
CCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEE
WGPGPWFDYGEFYRESPNAIRTVQYFDKARMEINRPDDGIVTNGLLVKELVSGRMQLGDF
ECCCCCCCHHHHHHCCCCCEEHHHHHHHHHEECCCCCCCEEECCHHHHHHHHCCCCCCCC
PYDVTYRDPSDVPVAGNPRAANTIAPGYRDFAGIATIDNGYRDPSRLNERVSAVIARGGN
CEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCC
IGIREDLARPETTIVQYNSVTGHNIPRVFWDFMNARGRVVENGRVVTAPIVDWLFAMGYP
CCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCEEEEHHHHHHHHHCCCC
ITDPYWTRAVVGDTERDVLVQLFERRVLTYTPDNPPGYQVEMGNVGQHYFQWRYPHLGAP
CCCCCCCEEEECCCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCC
WVAPDPATPLIYASNIDTGSHWELYRATFDGGGRRLTFNNGETVAFSWRRSWDPNQHFLV
CCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEEECCCCCCCEEEE
VDSRRNSPQYRQIYLLNAVAADAGEQAAGASVVRISYSNADGNFPPSDNDSSSIPGSEYN
EECCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
ASVSPDGTLLAFVSERQGIPQLYLRRLNVSPLRGYASPITAYDRPCNVESPTWSPDGRYL
CCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
FWVTNCEGNFEIYRATIVFYYIDDLFASISLNNIRNLSNNAANDRFARISPDGRTIAFAS
EEEECCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEC
DRDGNWEIYVMNSDGSNVRRLTNNAATDDSPTWSLTNQQLAFASDRDGDFEIYILNVSDG
CCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCEEEEEEECCCC
AIVQQVTQNTAQDRWPLWAQ
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYRPGMMWSMLALIGALMLSSVAPLAAAPFSGRTSFADPRFAAIWSRTDSEAVRGGRTWY
CCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCEEE
WGPGPWFDYGEFYRESPNAIRTVQYFDKARMEINRPDDGIVTNGLLVKELVSGRMQLGDF
ECCCCCCCHHHHHHCCCCCEEHHHHHHHHHEECCCCCCCEEECCHHHHHHHHCCCCCCCC
PYDVTYRDPSDVPVAGNPRAANTIAPGYRDFAGIATIDNGYRDPSRLNERVSAVIARGGN
CEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCC
IGIREDLARPETTIVQYNSVTGHNIPRVFWDFMNARGRVVENGRVVTAPIVDWLFAMGYP
CCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCEEEEHHHHHHHHHCCCC
ITDPYWTRAVVGDTERDVLVQLFERRVLTYTPDNPPGYQVEMGNVGQHYFQWRYPHLGAP
CCCCCCCEEEECCCHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCC
WVAPDPATPLIYASNIDTGSHWELYRATFDGGGRRLTFNNGETVAFSWRRSWDPNQHFLV
CCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEEECCCCCCCEEEE
VDSRRNSPQYRQIYLLNAVAADAGEQAAGASVVRISYSNADGNFPPSDNDSSSIPGSEYN
EECCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
ASVSPDGTLLAFVSERQGIPQLYLRRLNVSPLRGYASPITAYDRPCNVESPTWSPDGRYL
CCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
FWVTNCEGNFEIYRATIVFYYIDDLFASISLNNIRNLSNNAANDRFARISPDGRTIAFAS
EEEECCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEC
DRDGNWEIYVMNSDGSNVRRLTNNAATDDSPTWSLTNQQLAFASDRDGDFEIYILNVSDG
CCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCEEEEEEECCCC
AIVQQVTQNTAQDRWPLWAQ
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA