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The map label for this gene is hemL [H]

Identifier: 222527319

GI number: 222527319

Start: 5081666

End: 5082997

Strand: Direct

Name: hemL [H]

Synonym: Chy400_4104

Alternate gene names: 222527319

Gene position: 5081666-5082997 (Clockwise)

Preceding gene: 222527318

Following gene: 222527320

Centisome position: 96.45

GC content: 59.91

Gene sequence:

>1332_bases
ATGACACTCGAAGAGCAATATCGTCGGCGATTTGCCGGTTCAGCGACGCTCGCCGAGAAGGCACGTGTCCGCTTCCCCGG
TGGTGCAACCCACGACGGTCGTTACGCCGATCCCTTTCCTCTCTTCATGGAACGAGCCAGCGGGCCGTATAAGTGGGATG
TTGATGACAATCGGATGATCGATTACTGGTGCGGACACGGAGCCTTACTGCTCGGTCACGGCCATCCAACCATTGTCAAC
GCTATTCAGCAGCAAGCCCCCATCGCCACCCATCTCGGCGCCAATCATCGCCTTGAACTGGAATGGGCCGATCTGATCGC
CGATCTCATTCCGGCGGCTGAGCGCATTCGCTTCACCGCGTCGGGAAGTGAAGCAACAACCCTCGCTCTGCGCATTGCCC
GCGCAGCTACCGGCAAGACGACGATCATCCGCTTCGCGGGCCATTTTCACGGCTGGCACGACGCGCTTGCACCCGGTGCC
GATCCCGATGATCCCCACCGCGGAGTATTGCCGGCAACCCTCTCAAGTTTGCTGATCTTGCCGACCGATCTTGATCAGGT
GGCTGCGACGCTGGCTACGCATACCGACATTGCCGCGGTTATTCTCGAACCTACGGGTGCTTCCTACGGTTCGATCCCGC
TCCCGGATACGTTTCTGATCGGCTTACGTGAGCTTACTCAGCAATACGGTGTGCTCCTTATTGCCGACGAAGTCGTTACC
GGCTTTCGGGTTGCGCCCGGTGGTGCCTGCGCACGTGCCGGAGTACGCCCTGATCTGGTCTGTCTGGCCAAAATTGTCGC
CGGTGGATTGCCCGGCGGGGCGCTAGTCGGACGGGCTGACCTCCTCGACCTGCTGGCCCATGACGAGAATGGCCCCCGTG
GACGCATCCGCCAACAGGGAACATTCAACGCCAATCCTCTCTCTGCTGCTGCCGGCATTGCTATGCTACGTGCCGTCGCT
ACCGGCGAACCTGGTGCTATCGCCGCCGCACGTGGCACAATGCTCATCCAAATGCTCAACGAACTGTTTGCTTCGTATCG
TTTGCGTGGCTGGACAGCCTTCGGTGATGGATCGATCTTTCACCTGTTTGTCGATCCGGCAACCGACCTGATCCCCGGTG
AGATTCCGCGCAATCTGCCACTTGCCACGCTTAAACGCGGGGGTGATCCGCAAATCCTGCGCCGGCTGCGGATGGCGTTA
CACCTGAATGGTGTCGATTTGATGCGGGGGCGCAGTGGTTTTGTGTCGGCTGTTCATAGCGAAGAGGACATTCGGGCTAC
GGTGGCTGCGGTTGCCGCTGCTATCGAACTGGTGATGGCCGAACCCGCTTGA

Upstream 100 bases:

>100_bases
TTCAAACGGGTTGCCTACGATATTAAACAAGTTCAACAACAGATGCGCGATTACGGGTTACCACCCGCCCTTATCTATCG
CCTGAGTGTAGGAATGTGAT

Downstream 100 bases:

>100_bases
AATCCAGAAATAGCAGGTGATACACCTCTCTGCAACGTCACCTACTGCAACATGTAAGGAGTTTGTACCTGCAATGCGAG
TCCAATCGATCACGCTACCT

Product: class III aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTLEEQYRRRFAGSATLAEKARVRFPGGATHDGRYADPFPLFMERASGPYKWDVDDNRMIDYWCGHGALLLGHGHPTIVN
AIQQQAPIATHLGANHRLELEWADLIADLIPAAERIRFTASGSEATTLALRIARAATGKTTIIRFAGHFHGWHDALAPGA
DPDDPHRGVLPATLSSLLILPTDLDQVAATLATHTDIAAVILEPTGASYGSIPLPDTFLIGLRELTQQYGVLLIADEVVT
GFRVAPGGACARAGVRPDLVCLAKIVAGGLPGGALVGRADLLDLLAHDENGPRGRIRQQGTFNANPLSAAAGIAMLRAVA
TGEPGAIAAARGTMLIQMLNELFASYRLRGWTAFGDGSIFHLFVDPATDLIPGEIPRNLPLATLKRGGDPQILRRLRMAL
HLNGVDLMRGRSGFVSAVHSEEDIRATVAAVAAAIELVMAEPA

Sequences:

>Translated_443_residues
MTLEEQYRRRFAGSATLAEKARVRFPGGATHDGRYADPFPLFMERASGPYKWDVDDNRMIDYWCGHGALLLGHGHPTIVN
AIQQQAPIATHLGANHRLELEWADLIADLIPAAERIRFTASGSEATTLALRIARAATGKTTIIRFAGHFHGWHDALAPGA
DPDDPHRGVLPATLSSLLILPTDLDQVAATLATHTDIAAVILEPTGASYGSIPLPDTFLIGLRELTQQYGVLLIADEVVT
GFRVAPGGACARAGVRPDLVCLAKIVAGGLPGGALVGRADLLDLLAHDENGPRGRIRQQGTFNANPLSAAAGIAMLRAVA
TGEPGAIAAARGTMLIQMLNELFASYRLRGWTAFGDGSIFHLFVDPATDLIPGEIPRNLPLATLKRGGDPQILRRLRMAL
HLNGVDLMRGRSGFVSAVHSEEDIRATVAAVAAAIELVMAEPA
>Mature_442_residues
TLEEQYRRRFAGSATLAEKARVRFPGGATHDGRYADPFPLFMERASGPYKWDVDDNRMIDYWCGHGALLLGHGHPTIVNA
IQQQAPIATHLGANHRLELEWADLIADLIPAAERIRFTASGSEATTLALRIARAATGKTTIIRFAGHFHGWHDALAPGAD
PDDPHRGVLPATLSSLLILPTDLDQVAATLATHTDIAAVILEPTGASYGSIPLPDTFLIGLRELTQQYGVLLIADEVVTG
FRVAPGGACARAGVRPDLVCLAKIVAGGLPGGALVGRADLLDLLAHDENGPRGRIRQQGTFNANPLSAAAGIAMLRAVAT
GEPGAIAAARGTMLIQMLNELFASYRLRGWTAFGDGSIFHLFVDPATDLIPGEIPRNLPLATLKRGGDPQILRRLRMALH
LNGVDLMRGRSGFVSAVHSEEDIRATVAAVAAAIELVMAEPA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=322, Percent_Identity=29.8136645962733, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1786349, Length=432, Percent_Identity=35.1851851851852, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789016, Length=302, Percent_Identity=31.7880794701987, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI145693181, Length=336, Percent_Identity=30.952380952381, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788044, Length=326, Percent_Identity=30.6748466257669, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787560, Length=264, Percent_Identity=30.6818181818182, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789759, Length=312, Percent_Identity=29.8076923076923, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786991, Length=279, Percent_Identity=27.2401433691756, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25144271, Length=320, Percent_Identity=25.625, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323470, Length=449, Percent_Identity=24.4988864142539, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357415, Length=321, Percent_Identity=27.1028037383178, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356575, Length=366, Percent_Identity=27.5956284153005, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 47157; Mature: 47025

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEEQYRRRFAGSATLAEKARVRFPGGATHDGRYADPFPLFMERASGPYKWDVDDNRMI
CCCHHHHHHHHCCCCHHHHHCEEECCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCEEE
DYWCGHGALLLGHGHPTIVNAIQQQAPIATHLGANHRLELEWADLIADLIPAAERIRFTA
EEECCCCEEEEECCCHHHHHHHHHHCCCHHHCCCCCEEEEHHHHHHHHHHCHHHHEEEEE
SGSEATTLALRIARAATGKTTIIRFAGHFHGWHDALAPGADPDDPHRGVLPATLSSLLIL
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHHHCCEEE
PTDLDQVAATLATHTDIAAVILEPTGASYGSIPLPDTFLIGLRELTQQYGVLLIADEVVT
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHC
GFRVAPGGACARAGVRPDLVCLAKIVAGGLPGGALVGRADLLDLLAHDENGPRGRIRQQG
CEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
TFNANPLSAAAGIAMLRAVATGEPGAIAAARGTMLIQMLNELFASYRLRGWTAFGDGSIF
CCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHECCEEEEECCCCEE
HLFVDPATDLIPGEIPRNLPLATLKRGGDPQILRRLRMALHLNGVDLMRGRSGFVSAVHS
EEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCC
EEDIRATVAAVAAAIELVMAEPA
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLEEQYRRRFAGSATLAEKARVRFPGGATHDGRYADPFPLFMERASGPYKWDVDDNRMI
CCHHHHHHHHCCCCHHHHHCEEECCCCCCCCCCCCCCHHHHHHHCCCCEEECCCCCEEE
DYWCGHGALLLGHGHPTIVNAIQQQAPIATHLGANHRLELEWADLIADLIPAAERIRFTA
EEECCCCEEEEECCCHHHHHHHHHHCCCHHHCCCCCEEEEHHHHHHHHHHCHHHHEEEEE
SGSEATTLALRIARAATGKTTIIRFAGHFHGWHDALAPGADPDDPHRGVLPATLSSLLIL
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHHHCCEEE
PTDLDQVAATLATHTDIAAVILEPTGASYGSIPLPDTFLIGLRELTQQYGVLLIADEVVT
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHC
GFRVAPGGACARAGVRPDLVCLAKIVAGGLPGGALVGRADLLDLLAHDENGPRGRIRQQG
CEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
TFNANPLSAAAGIAMLRAVATGEPGAIAAARGTMLIQMLNELFASYRLRGWTAFGDGSIF
CCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHECCEEEEECCCCEE
HLFVDPATDLIPGEIPRNLPLATLKRGGDPQILRRLRMALHLNGVDLMRGRSGFVSAVHS
EEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCC
EEDIRATVAAVAAAIELVMAEPA
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA