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The map label for this gene is odhB [H]

Identifier: 222527234

GI number: 222527234

Start: 4963633

End: 4964979

Strand: Reverse

Name: odhB [H]

Synonym: Chy400_4018

Alternate gene names: 222527234

Gene position: 4964979-4963633 (Counterclockwise)

Preceding gene: 222527235

Following gene: 222527232

Centisome position: 94.23

GC content: 59.69

Gene sequence:

>1347_bases
ATGGCATACGAAATTCGCGTACCGTCACTCGGTGAGTCGATTGTTGAGGCGACGGTAGCACGCTGGCTTAAGCGTGAAGG
TGAACCGGTTGCGGTTGGTGAACCGGTAGTTGAATTGGAGACCGATAAGGTTAATCTCGAAGTAGCCGCCGATCAGAGCG
GGATTCTGACCAGCATCGCATGTCCAGAAGGGACTACGGTTGGTATTGGCGATCTGCTGGGTACGATTGAAGCCGGGGCA
TTGCCTAAAGAAGCACCGGTGGCGGCAGCCAGTACAACAACGGTAGCGGCCCCTGTCGCTGAACCGGTAGCTGCTACTGC
GGTCAATGAAGTGCTGGCGACACCGGTTGCCCAGCGCATGGCTGCCGAACACGCTATCGATTTGCGCACAGTTCCCGGCA
CCGGCCCCGGCGGGCGCATTACTAAAGAAGATGTTATGCGGCTGGTGTCGGGTACCGGGCCGAGTGAAGCAACCGCTAAA
GCGGATGAAGCGCGGGTCCACGTCAGCCATCCGACCCCGGCTGTGGTAGAGCGCCCACAGCCCGCTCAGCCGTCACCTGC
GCCAGCTCCGGTTGCGCCGCCACCACCAGCACCGGCCCCACGGCCAACGCCACCTGCAGCCCCGGCAGTAGTCAGTACGA
ATGGTGACCGTCGTGAAGAACGCCAGCGCCTGAGTCGGCGCCGTTTAACCATCGCTCGCCGGCTGGTGGAAGCGCAGCAC
ACCGCAGCGATGCTTACCACCTTCAACGAAGTGGATATGAGCGCGGTGATGGCGTTGCGCGCCCGTCACAAGGACTCGTT
CAAAGAGCGGCATGGGGTTGGTTTGGGCTTTATGTCCTTCTTCACCAAAGCCGTGGTCGGAGCGCTCAAAGCTTTTCCGA
TGGTAAATGCCGAGATTCAGGGCGAAGAGGTCGTCATCAAGTACTACTACGACATCGGCATCGCCGTTGGTGTTGACGAG
GGGCTGGTGGTGCCGGTTGTGCGCGATGCCGACCGTAAGACCTTCGCACAGATCGAGCGCGAAATCGCCCAACTGGCGAA
GAAGGCACGGGAAGGAACGTTGAGTCTGGCCGAACTTCAGGGGGGCACATTCACGATCACGAATGGCGGTGTCTACGGTT
CGCTCATGAGCACGCCGATTCTCAATGCCCCGCAAGTAGGCATTCTGGGCATGCACAAAATCGAAGAGCGTCCGGTCGTC
GTCGGCGGGCAGATCGTCATTCGCCCGATGATGTACGTTGCGCTGAGCTACGACCATCGCTTGATCGATGGCAGCACAGC
AGTGCGCTTCCTGGTGAAAGTGAAAGAGCTGATTGAAGACCCAGAGGCGTTGCTGCTGGAAGGGTAG

Upstream 100 bases:

>100_bases
GTAGCTGATCTGCCGGAGTCTCCTGTACCGGCATTGCAAGGACGAGAAACGATGACGCGCTAAGGTATGTGCGTTACTGA
TGAGTGAGGTAGGTTAGGGC

Downstream 100 bases:

>100_bases
CTAATCCAACCATCAAGAGGAACGGGGGCGGCTACGCGAGTCGCCCCCGTTGTGTTTTCGCAACCTCCCGAAAGACACTC
AGCGCGTAAGCCGCCCAGAA

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAYEIRVPSLGESIVEATVARWLKREGEPVAVGEPVVELETDKVNLEVAADQSGILTSIACPEGTTVGIGDLLGTIEAGA
LPKEAPVAAASTTTVAAPVAEPVAATAVNEVLATPVAQRMAAEHAIDLRTVPGTGPGGRITKEDVMRLVSGTGPSEATAK
ADEARVHVSHPTPAVVERPQPAQPSPAPAPVAPPPPAPAPRPTPPAAPAVVSTNGDRREERQRLSRRRLTIARRLVEAQH
TAAMLTTFNEVDMSAVMALRARHKDSFKERHGVGLGFMSFFTKAVVGALKAFPMVNAEIQGEEVVIKYYYDIGIAVGVDE
GLVVPVVRDADRKTFAQIEREIAQLAKKAREGTLSLAELQGGTFTITNGGVYGSLMSTPILNAPQVGILGMHKIEERPVV
VGGQIVIRPMMYVALSYDHRLIDGSTAVRFLVKVKELIEDPEALLLEG

Sequences:

>Translated_448_residues
MAYEIRVPSLGESIVEATVARWLKREGEPVAVGEPVVELETDKVNLEVAADQSGILTSIACPEGTTVGIGDLLGTIEAGA
LPKEAPVAAASTTTVAAPVAEPVAATAVNEVLATPVAQRMAAEHAIDLRTVPGTGPGGRITKEDVMRLVSGTGPSEATAK
ADEARVHVSHPTPAVVERPQPAQPSPAPAPVAPPPPAPAPRPTPPAAPAVVSTNGDRREERQRLSRRRLTIARRLVEAQH
TAAMLTTFNEVDMSAVMALRARHKDSFKERHGVGLGFMSFFTKAVVGALKAFPMVNAEIQGEEVVIKYYYDIGIAVGVDE
GLVVPVVRDADRKTFAQIEREIAQLAKKAREGTLSLAELQGGTFTITNGGVYGSLMSTPILNAPQVGILGMHKIEERPVV
VGGQIVIRPMMYVALSYDHRLIDGSTAVRFLVKVKELIEDPEALLLEG
>Mature_447_residues
AYEIRVPSLGESIVEATVARWLKREGEPVAVGEPVVELETDKVNLEVAADQSGILTSIACPEGTTVGIGDLLGTIEAGAL
PKEAPVAAASTTTVAAPVAEPVAATAVNEVLATPVAQRMAAEHAIDLRTVPGTGPGGRITKEDVMRLVSGTGPSEATAKA
DEARVHVSHPTPAVVERPQPAQPSPAPAPVAPPPPAPAPRPTPPAAPAVVSTNGDRREERQRLSRRRLTIARRLVEAQHT
AAMLTTFNEVDMSAVMALRARHKDSFKERHGVGLGFMSFFTKAVVGALKAFPMVNAEIQGEEVVIKYYYDIGIAVGVDEG
LVVPVVRDADRKTFAQIEREIAQLAKKAREGTLSLAELQGGTFTITNGGVYGSLMSTPILNAPQVGILGMHKIEERPVVV
GGQIVIRPMMYVALSYDHRLIDGSTAVRFLVKVKELIEDPEALLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=235, Percent_Identity=55.7446808510638, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=30.8855291576674, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=30.8855291576674, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI31711992, Length=477, Percent_Identity=27.6729559748428, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=27.4944567627494, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI260898739, Length=164, Percent_Identity=33.5365853658537, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=462, Percent_Identity=29.8701298701299, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1786946, Length=145, Percent_Identity=40, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25146366, Length=343, Percent_Identity=41.1078717201166, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17537937, Length=455, Percent_Identity=27.6923076923077, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=486, Percent_Identity=25.9259259259259, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=349, Percent_Identity=27.7936962750716, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=445, Percent_Identity=39.7752808988764, Blast_Score=293, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6324258, Length=173, Percent_Identity=30.0578034682081, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=52.991452991453, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859875, Length=469, Percent_Identity=27.5053304904051, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=31.4159292035398, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=30.2127659574468, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 47459; Mature: 47328

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYEIRVPSLGESIVEATVARWLKREGEPVAVGEPVVELETDKVNLEVAADQSGILTSIA
CEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCEEEEEEECCCCCCEEEEE
CPEGTTVGIGDLLGTIEAGALPKEAPVAAASTTTVAAPVAEPVAATAVNEVLATPVAQRM
CCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
AAEHAIDLRTVPGTGPGGRITKEDVMRLVSGTGPSEATAKADEARVHVSHPTPAVVERPQ
HHHHCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCEEEECCCCCHHCCCCC
PAQPSPAPAPVAPPPPAPAPRPTPPAAPAVVSTNGDRREERQRLSRRRLTIARRLVEAQH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAAMLTTFNEVDMSAVMALRARHKDSFKERHGVGLGFMSFFTKAVVGALKAFPMVNAEIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEC
GEEVVIKYYYDIGIAVGVDEGLVVPVVRDADRKTFAQIEREIAQLAKKAREGTLSLAELQ
CCEEEEEEEEECEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GGTFTITNGGVYGSLMSTPILNAPQVGILGMHKIEERPVVVGGQIVIRPMMYVALSYDHR
CCEEEEECCCEEEHHHHCCCCCCCCCCEECHHHHCCCCEEECCHHHHHHHHHHHHCCCCE
LIDGSTAVRFLVKVKELIEDPEALLLEG
EECCHHHHHHHHHHHHHHCCCHHEEECC
>Mature Secondary Structure 
AYEIRVPSLGESIVEATVARWLKREGEPVAVGEPVVELETDKVNLEVAADQSGILTSIA
EEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCEEEEEEECCCCCCEEEEE
CPEGTTVGIGDLLGTIEAGALPKEAPVAAASTTTVAAPVAEPVAATAVNEVLATPVAQRM
CCCCCEECHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
AAEHAIDLRTVPGTGPGGRITKEDVMRLVSGTGPSEATAKADEARVHVSHPTPAVVERPQ
HHHHCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCEEEECCCCCHHCCCCC
PAQPSPAPAPVAPPPPAPAPRPTPPAAPAVVSTNGDRREERQRLSRRRLTIARRLVEAQH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAAMLTTFNEVDMSAVMALRARHKDSFKERHGVGLGFMSFFTKAVVGALKAFPMVNAEIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEC
GEEVVIKYYYDIGIAVGVDEGLVVPVVRDADRKTFAQIEREIAQLAKKAREGTLSLAELQ
CCEEEEEEEEECEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GGTFTITNGGVYGSLMSTPILNAPQVGILGMHKIEERPVVVGGQIVIRPMMYVALSYDHR
CCEEEEECCCEEEHHHHCCCCCCCCCCEECHHHHCCCCEEECCHHHHHHHHHHHHCCCCE
LIDGSTAVRFLVKVKELIEDPEALLLEG
EECCHHHHHHHHHHHHHHCCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]