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The map label for this gene is sigA [H]

Identifier: 222527085

GI number: 222527085

Start: 4809911

End: 4811107

Strand: Direct

Name: sigA [H]

Synonym: Chy400_3864

Alternate gene names: 222527085

Gene position: 4809911-4811107 (Clockwise)

Preceding gene: 222527084

Following gene: 222527090

Centisome position: 91.29

GC content: 58.56

Gene sequence:

>1197_bases
ATGACGCAGAGCCAGCCCGAGACCGACCGCGCCGCTCCTGCTATTAATCCCGCAGATTTGCAGGCACTGATCGCGCAGGG
ACGGCAACAAGGCTTCGTTACATTTGAGGATATTCAGCGGCTGGTTCCCAACCCTGATGAGTCGATTGAATTGATCGACT
CCATCTATGCCGCATTAGCCGAGGCCGGTGTTCCGGTTCAGGAAGGTGATGAAACAGCTCTGGACGATGAGCCGTCATCA
CCTGCCACAATGGACCTCGATCTCGATGACGAGTTGTCAGACGCATTGCTCAGCGATAGCGTCCGCCTCTATCTGCGCGA
AATTGGTCAGGTTCCCCTGCTCACCGCAGAACAAGAGAAGCGACTCGCGCAGTTGATCGAGCAGGGCCAGGAGGCCGAGC
GCAAGCTGGCGACGCTGCCACCCGATAGCCCGGAAGCAGCCCGCTTGAAACAATTGAAGGCGAAGGGTGAAGAGGCGCGA
CAGCAAATGGCAGCCGCTAATCTGCGCCTCGTGGTTAGTATTGCCAAACGCTACCGTGACCGTGGCCTGCCCCTCCTCGA
TCTCATTCAAGAAGGCAGCCTCGGATTACTGCGTGCCATCGAAAAATTTGATTACACCAAAGGCTACAAGTTCAGTACAT
ACGCCACATGGTGGATCAAGCAGGCACTTTCCCGGGCCCTCGCCGATCAGTCACGCCTGGTACGACTCCCGGTGCATCTT
GGCGAAACCCTTAACCGGATTCAGTCGGCCCGTCGGCAACTCACCCAATCGCTCGGTCGTGAACCAACCGATACTGAGCT
GGCGAACCATCTTGGGATGAGCGAAGAGAAGCTGCGCGAGCTGCGCCGTACTGCCCAAGACCCGGTATCGCTGGCTACAC
CGGTCGGCGAAGAGGCCGACAGTACGCTGGCCGACTTCATTCCCGATCCGCACGCGCTCGATGCCGATGATGCTGCTGCC
AGCGGCATGCTGCGCCAGCAGATTACGGCTGCTCTCGATCAGCTCAGCGAACGTGAGCGACGGGTGCTTGAGTTGCGTTA
CGGCCTGGTTGATGGTCAGCCGCGCACGCTCGAAGAGGTCGGTAAAGCGTTTGGCGTTACCCGCGAGCGGGTACGGCAGA
TTGAAGTCAAGGCGCTGCGCAAGTTGCGCCATCCACGGTTGGGCAAGCTGCTGAAGGATTACCTCGATCAAATTTGA

Upstream 100 bases:

>100_bases
CCCATTCCGTTCTTGGCCTGCGCGGATAGCAGCTTGTGCTACAATGGAGGAACGAACGATAGCTTGCTATTGCCTGTGAG
GTCTCAGACGAGGAAGTAGT

Downstream 100 bases:

>100_bases
GGGTTTGCGGGTACGTTGGGGCACGTCGCGACGTGCCCATATTTTATTGACAGGCTAGAACCTCCCAGCCTGCCGGTATG
GCAGCGCAGAGTATGACCAT

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTQSQPETDRAAPAINPADLQALIAQGRQQGFVTFEDIQRLVPNPDESIELIDSIYAALAEAGVPVQEGDETALDDEPSS
PATMDLDLDDELSDALLSDSVRLYLREIGQVPLLTAEQEKRLAQLIEQGQEAERKLATLPPDSPEAARLKQLKAKGEEAR
QQMAAANLRLVVSIAKRYRDRGLPLLDLIQEGSLGLLRAIEKFDYTKGYKFSTYATWWIKQALSRALADQSRLVRLPVHL
GETLNRIQSARRQLTQSLGREPTDTELANHLGMSEEKLRELRRTAQDPVSLATPVGEEADSTLADFIPDPHALDADDAAA
SGMLRQQITAALDQLSERERRVLELRYGLVDGQPRTLEEVGKAFGVTRERVRQIEVKALRKLRHPRLGKLLKDYLDQI

Sequences:

>Translated_398_residues
MTQSQPETDRAAPAINPADLQALIAQGRQQGFVTFEDIQRLVPNPDESIELIDSIYAALAEAGVPVQEGDETALDDEPSS
PATMDLDLDDELSDALLSDSVRLYLREIGQVPLLTAEQEKRLAQLIEQGQEAERKLATLPPDSPEAARLKQLKAKGEEAR
QQMAAANLRLVVSIAKRYRDRGLPLLDLIQEGSLGLLRAIEKFDYTKGYKFSTYATWWIKQALSRALADQSRLVRLPVHL
GETLNRIQSARRQLTQSLGREPTDTELANHLGMSEEKLRELRRTAQDPVSLATPVGEEADSTLADFIPDPHALDADDAAA
SGMLRQQITAALDQLSERERRVLELRYGLVDGQPRTLEEVGKAFGVTRERVRQIEVKALRKLRHPRLGKLLKDYLDQI
>Mature_397_residues
TQSQPETDRAAPAINPADLQALIAQGRQQGFVTFEDIQRLVPNPDESIELIDSIYAALAEAGVPVQEGDETALDDEPSSP
ATMDLDLDDELSDALLSDSVRLYLREIGQVPLLTAEQEKRLAQLIEQGQEAERKLATLPPDSPEAARLKQLKAKGEEARQ
QMAAANLRLVVSIAKRYRDRGLPLLDLIQEGSLGLLRAIEKFDYTKGYKFSTYATWWIKQALSRALADQSRLVRLPVHLG
ETLNRIQSARRQLTQSLGREPTDTELANHLGMSEEKLRELRRTAQDPVSLATPVGEEADSTLADFIPDPHALDADDAAAS
GMLRQQITAALDQLSERERRVLELRYGLVDGQPRTLEEVGKAFGVTRERVRQIEVKALRKLRHPRLGKLLKDYLDQI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=51.004016064257, Blast_Score=270, Evalue=9e-74,
Organism=Escherichia coli, GI1789098, Length=328, Percent_Identity=39.0243902439024, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789871, Length=298, Percent_Identity=28.1879194630872, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788231, Length=212, Percent_Identity=30.188679245283, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 44389; Mature: 44258

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSQPETDRAAPAINPADLQALIAQGRQQGFVTFEDIQRLVPNPDESIELIDSIYAALA
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
EAGVPVQEGDETALDDEPSSPATMDLDLDDELSDALLSDSVRLYLREIGQVPLLTAEQEK
HCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
RLAQLIEQGQEAERKLATLPPDSPEAARLKQLKAKGEEARQQMAAANLRLVVSIAKRYRD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLPLLDLIQEGSLGLLRAIEKFDYTKGYKFSTYATWWIKQALSRALADQSRLVRLPVHL
CCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHH
GETLNRIQSARRQLTQSLGREPTDTELANHLGMSEEKLRELRRTAQDPVSLATPVGEEAD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCHHH
STLADFIPDPHALDADDAAASGMLRQQITAALDQLSERERRVLELRYGLVDGQPRTLEEV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GKAFGVTRERVRQIEVKALRKLRHPRLGKLLKDYLDQI
HHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TQSQPETDRAAPAINPADLQALIAQGRQQGFVTFEDIQRLVPNPDESIELIDSIYAALA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
EAGVPVQEGDETALDDEPSSPATMDLDLDDELSDALLSDSVRLYLREIGQVPLLTAEQEK
HCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
RLAQLIEQGQEAERKLATLPPDSPEAARLKQLKAKGEEARQQMAAANLRLVVSIAKRYRD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLPLLDLIQEGSLGLLRAIEKFDYTKGYKFSTYATWWIKQALSRALADQSRLVRLPVHL
CCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHH
GETLNRIQSARRQLTQSLGREPTDTELANHLGMSEEKLRELRRTAQDPVSLATPVGEEAD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCHHH
STLADFIPDPHALDADDAAASGMLRQQITAALDQLSERERRVLELRYGLVDGQPRTLEEV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GKAFGVTRERVRQIEVKALRKLRHPRLGKLLKDYLDQI
HHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]