Click here to switch to the map view.

The map label for this gene is lasT [H]

Identifier: 222527064

GI number: 222527064

Start: 4788785

End: 4789504

Strand: Direct

Name: lasT [H]

Synonym: Chy400_3843

Alternate gene names: 222527064

Gene position: 4788785-4789504 (Clockwise)

Preceding gene: 222527063

Following gene: 222527065

Centisome position: 90.89

GC content: 60.56

Gene sequence:

>720_bases
ATGAACGACATTATTGTCATTCTCCATCAACCGCGTGATCCGCGCAACATCGGCGCAGTGGTACGGGCAATGCTCAACAC
CGGCTGCCACCACCTGCGCCTGGTCGATCCCTGCCCGTTCGATCCCGCGACGATTAGCGCAGTTGCCCATCGCCCCGATC
CCGTGCTGGCGAATCTCACCGTCCACGCACATCTATCGGAAGCTATCGCCGATATACGCTACCTGGTTGGCACCAGCGAC
CGCCCCCACCCCGGTCTGCCGTGGCGCAACGATGTCCGGCAATGGGCCAACGAAACCCGTCAACGCGCTGCCAGTACCGG
CCCGGTCGCCATTCTTTTCGGTAGCGAAGGGAATGGCCTGAGTCGGGACGAATTAGTTTTATGTCAAGAAATTCTCGGCC
TGCCGATGGCGTCTGACTATCCCGTCCTCAACCTGGCGCAGGCTGTTTTGCTTGTCTTGTATGAACTACAACAGGTGACG
ACTCCTCTTGCGCCACCTCCCCCGCCTGGTGAACCCCCGGCTACCCTGGCCGCACGTGAGAGTCTGTATACCGCTGTCGC
AGATCTGATCAGCACCACCAACTTTGTCAAGAGTGGCACTGGACAGGCACTGCATCAGCGCCTGCGCACCATCATTGCCC
GCGCCGACCTCAGCGAGCGCGATGCTGCCGTGCTGACGGCACTGCTACGCGAGGTGCTGAGGAAGTTGGAGAGGAGGTAG

Upstream 100 bases:

>100_bases
ATGTCAGCCCCGAAACCATTCGCCGCGATCTCTCCGATCTGGTCAGCCGTGGCCTACTCTTGAAAGTCGGCGATAAACGG
GCCACCTACTACATTCTCAA

Downstream 100 bases:

>100_bases
GGTGTTAGGAGATAGAAGATAGGAGATAGAAGATAGGAGATAGAAGATAGGAGATAGAAGATAGGAGATAGGAGATAGAA
GATAGGAGATAGGAAAGAGG

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNDIIVILHQPRDPRNIGAVVRAMLNTGCHHLRLVDPCPFDPATISAVAHRPDPVLANLTVHAHLSEAIADIRYLVGTSD
RPHPGLPWRNDVRQWANETRQRAASTGPVAILFGSEGNGLSRDELVLCQEILGLPMASDYPVLNLAQAVLLVLYELQQVT
TPLAPPPPPGEPPATLAARESLYTAVADLISTTNFVKSGTGQALHQRLRTIIARADLSERDAAVLTALLREVLRKLERR

Sequences:

>Translated_239_residues
MNDIIVILHQPRDPRNIGAVVRAMLNTGCHHLRLVDPCPFDPATISAVAHRPDPVLANLTVHAHLSEAIADIRYLVGTSD
RPHPGLPWRNDVRQWANETRQRAASTGPVAILFGSEGNGLSRDELVLCQEILGLPMASDYPVLNLAQAVLLVLYELQQVT
TPLAPPPPPGEPPATLAARESLYTAVADLISTTNFVKSGTGQALHQRLRTIIARADLSERDAAVLTALLREVLRKLERR
>Mature_239_residues
MNDIIVILHQPRDPRNIGAVVRAMLNTGCHHLRLVDPCPFDPATISAVAHRPDPVLANLTVHAHLSEAIADIRYLVGTSD
RPHPGLPWRNDVRQWANETRQRAASTGPVAILFGSEGNGLSRDELVLCQEILGLPMASDYPVLNLAQAVLLVLYELQQVT
TPLAPPPPPGEPPATLAARESLYTAVADLISTTNFVKSGTGQALHQRLRTIIARADLSERDAAVLTALLREVLRKLERR

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=241, Percent_Identity=30.7053941908714, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788881, Length=237, Percent_Identity=28.6919831223629, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26082; Mature: 26082

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIIVILHQPRDPRNIGAVVRAMLNTGCHHLRLVDPCPFDPATISAVAHRPDPVLANLT
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEHH
VHAHLSEAIADIRYLVGTSDRPHPGLPWRNDVRQWANETRQRAASTGPVAILFGSEGNGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
SRDELVLCQEILGLPMASDYPVLNLAQAVLLVLYELQQVTTPLAPPPPPGEPPATLAARE
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
SLYTAVADLISTTNFVKSGTGQALHQRLRTIIARADLSERDAAVLTALLREVLRKLERR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDIIVILHQPRDPRNIGAVVRAMLNTGCHHLRLVDPCPFDPATISAVAHRPDPVLANLT
CCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEHH
VHAHLSEAIADIRYLVGTSDRPHPGLPWRNDVRQWANETRQRAASTGPVAILFGSEGNGL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
SRDELVLCQEILGLPMASDYPVLNLAQAVLLVLYELQQVTTPLAPPPPPGEPPATLAARE
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
SLYTAVADLISTTNFVKSGTGQALHQRLRTIIARADLSERDAAVLTALLREVLRKLERR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]