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The map label for this gene is phrB [H]

Identifier: 222527000

GI number: 222527000

Start: 4709973

End: 4711412

Strand: Direct

Name: phrB [H]

Synonym: Chy400_3778

Alternate gene names: 222527000

Gene position: 4709973-4711412 (Clockwise)

Preceding gene: 222526998

Following gene: 222527001

Centisome position: 89.39

GC content: 60.97

Gene sequence:

>1440_bases
ATGTACATTCACTGGTTTCGCCGTGATTTGCGTTTGCGCGATAACACGGCACTGATGGCAGCGGCAACTGCTGCTGGTGG
AGCGGTGGTGCCGGTCTTTATTTTCGATGACGCTATTCTGCGTGGGCGCTTTGCCAGCCCGGCACGCACGCAGTTCTTGC
TCGATTGTCTGGCGGCGCTCGACGCAGAACTGCGCACGTTTGGGTTGCATCTGGTGGTGCGCCGGGGTGACCCACTGCGA
ACGCTGTTTGATGTGCTGCGCGAGAGTGGTGCGAGCGGGGTGACCTGGAATCGTGACTATACGCCATACGCGGTTCGCCG
TGACACCGCGATCAAGCAGGCGCTGCGTGAGGCCGGTTATGAAGCGCATAGTTTCAAGGATACGGTCATTTTTGAGATGA
AGGAAGTGGCGACTGCCGATGGCCGTCCGTATACGGTCTACACCCCGTATGCGAAACGCTGGCGATCACGCCTGGCCGCC
GAACCGGTTACAGTGCAGGATATGCCACGGCTAGCGACGATTCCGTTGCCGGTCAGTGAGCCGCTGCCTCATCTGACCGA
CCTGCTGCCGGATGCGCCGGCGACACTCCCCCGCTTTCCCGCCGGTGAAGCCGTTGCGCTGGAAGCGCTCGAACGATTCG
TGCGTGGCCCACTGGCCAGTTATGCGCAGGGGCGTGATCTGATGGCCGTTGCCGGGACTTCGCGGCTCTCGCCGTACCTG
CGTCTGGGTGTGTTGTCACCGCGTCAGTGTGTCGCCGCAGCCCTCGCAGCGCCACCAGGGCCGGGGCCGGAGAGCTGGAT
CGGTGAATTGATCTGGCGTGATTTCTACGTGCAGGTGCTCTACCATTTTCCGCACGCCCTGCGCGGCAGCTTCAAACCGG
CGTATAACCGGATTGACTGGCCGAACGATCCGGTGCTGTTCGCCGCCTGGCAACAGGGGCTGACCGGCTACCCAATCGTT
GATGCGGCGATGCGTCAGTTGCAGCGCGAAGGCTGGATGCACAACCGGGCCAGAATGATTGTGGCCTCGTTTCTCACCAA
AGATTTGCTGATTGACTGGCGCTGGGGTGAACGCCATTTTATGCATCTGCTGATTGATGGCGATCCGGCGGCCAACAACG
GCGGCTGGCAGTGGGCAGCCGGTACCGGCACTGATGCTCAACCCTTCTTTCGCATCTTCAATCCGGTCAGTCAGGGGCAG
AAGTTCGATCCCGAAGGTGCGTATGTGCGGCGTTACGTGCCCGAACTGGTCAACGTGCCGACCCGTTACATCCACGAACC
GCATAAGATGTCGCTCGCCGAACAGCGCAAAGCCGGGGTGTTAATCGGGCGGGACTATCCGGCGCCGATTGTCGATCACG
CCACGCAGCGCACCAAAGCGCTCGAACTCTATCGGGCGGCAACGCGCCATAACGACTCATCAGCAGAACAGTATGCTTGA

Upstream 100 bases:

>100_bases
TCAAACACTCTCTACCTGTAAGGCGGTATCATACGGGCCAGGGGCGTATCCGGCGTATAATGTAAGGTGTGGATATGAAG
CTATAACTCAACCAAACCAT

Downstream 100 bases:

>100_bases
CGATCACACCCAATTGGAATTTCCGGCCAGTGGCATGACGGAACTGCCGCTCATCTGGCAACCGCAGGTGATCCGCTGCA
AGGCTGCCGGCCCTGGTGGT

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MYIHWFRRDLRLRDNTALMAAATAAGGAVVPVFIFDDAILRGRFASPARTQFLLDCLAALDAELRTFGLHLVVRRGDPLR
TLFDVLRESGASGVTWNRDYTPYAVRRDTAIKQALREAGYEAHSFKDTVIFEMKEVATADGRPYTVYTPYAKRWRSRLAA
EPVTVQDMPRLATIPLPVSEPLPHLTDLLPDAPATLPRFPAGEAVALEALERFVRGPLASYAQGRDLMAVAGTSRLSPYL
RLGVLSPRQCVAAALAAPPGPGPESWIGELIWRDFYVQVLYHFPHALRGSFKPAYNRIDWPNDPVLFAAWQQGLTGYPIV
DAAMRQLQREGWMHNRARMIVASFLTKDLLIDWRWGERHFMHLLIDGDPAANNGGWQWAAGTGTDAQPFFRIFNPVSQGQ
KFDPEGAYVRRYVPELVNVPTRYIHEPHKMSLAEQRKAGVLIGRDYPAPIVDHATQRTKALELYRAATRHNDSSAEQYA

Sequences:

>Translated_479_residues
MYIHWFRRDLRLRDNTALMAAATAAGGAVVPVFIFDDAILRGRFASPARTQFLLDCLAALDAELRTFGLHLVVRRGDPLR
TLFDVLRESGASGVTWNRDYTPYAVRRDTAIKQALREAGYEAHSFKDTVIFEMKEVATADGRPYTVYTPYAKRWRSRLAA
EPVTVQDMPRLATIPLPVSEPLPHLTDLLPDAPATLPRFPAGEAVALEALERFVRGPLASYAQGRDLMAVAGTSRLSPYL
RLGVLSPRQCVAAALAAPPGPGPESWIGELIWRDFYVQVLYHFPHALRGSFKPAYNRIDWPNDPVLFAAWQQGLTGYPIV
DAAMRQLQREGWMHNRARMIVASFLTKDLLIDWRWGERHFMHLLIDGDPAANNGGWQWAAGTGTDAQPFFRIFNPVSQGQ
KFDPEGAYVRRYVPELVNVPTRYIHEPHKMSLAEQRKAGVLIGRDYPAPIVDHATQRTKALELYRAATRHNDSSAEQYA
>Mature_479_residues
MYIHWFRRDLRLRDNTALMAAATAAGGAVVPVFIFDDAILRGRFASPARTQFLLDCLAALDAELRTFGLHLVVRRGDPLR
TLFDVLRESGASGVTWNRDYTPYAVRRDTAIKQALREAGYEAHSFKDTVIFEMKEVATADGRPYTVYTPYAKRWRSRLAA
EPVTVQDMPRLATIPLPVSEPLPHLTDLLPDAPATLPRFPAGEAVALEALERFVRGPLASYAQGRDLMAVAGTSRLSPYL
RLGVLSPRQCVAAALAAPPGPGPESWIGELIWRDFYVQVLYHFPHALRGSFKPAYNRIDWPNDPVLFAAWQQGLTGYPIV
DAAMRQLQREGWMHNRARMIVASFLTKDLLIDWRWGERHFMHLLIDGDPAANNGGWQWAAGTGTDAQPFFRIFNPVSQGQ
KFDPEGAYVRRYVPELVNVPTRYIHEPHKMSLAEQRKAGVLIGRDYPAPIVDHATQRTKALELYRAATRHNDSSAEQYA

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=492, Percent_Identity=33.130081300813, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI4758072, Length=501, Percent_Identity=32.5349301397206, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI188536103, Length=452, Percent_Identity=32.7433628318584, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1786926, Length=481, Percent_Identity=41.995841995842, Blast_Score=349, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6324962, Length=509, Percent_Identity=31.041257367387, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI17137248, Length=496, Percent_Identity=33.4677419354839, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24585455, Length=496, Percent_Identity=33.4677419354839, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24648152, Length=498, Percent_Identity=29.1164658634538, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53856; Mature: 53856

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIHWFRRDLRLRDNTALMAAATAAGGAVVPVFIFDDAILRGRFASPARTQFLLDCLAAL
CEEHEEHHHCCCCCCCEEEEEECCCCCCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHH
DAELRTFGLHLVVRRGDPLRTLFDVLRESGASGVTWNRDYTPYAVRRDTAIKQALREAGY
HHHHHHEEEEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCC
EAHSFKDTVIFEMKEVATADGRPYTVYTPYAKRWRSRLAAEPVTVQDMPRLATIPLPVSE
CHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCCHHCCCCEEECCCCCCC
PLPHLTDLLPDAPATLPRFPAGEAVALEALERFVRGPLASYAQGRDLMAVAGTSRLSPYL
CCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCHHCCHHH
RLGVLSPRQCVAAALAAPPGPGPESWIGELIWRDFYVQVLYHFPHALRGSFKPAYNRIDW
HCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PNDPVLFAAWQQGLTGYPIVDAAMRQLQREGWMHNRARMIVASFLTKDLLIDWRWGERHF
CCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCEE
MHLLIDGDPAANNGGWQWAAGTGTDAQPFFRIFNPVSQGQKFDPEGAYVRRYVPELVNVP
EEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
TRYIHEPHKMSLAEQRKAGVLIGRDYPAPIVDHATQRTKALELYRAATRHNDSSAEQYA
HHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MYIHWFRRDLRLRDNTALMAAATAAGGAVVPVFIFDDAILRGRFASPARTQFLLDCLAAL
CEEHEEHHHCCCCCCCEEEEEECCCCCCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHH
DAELRTFGLHLVVRRGDPLRTLFDVLRESGASGVTWNRDYTPYAVRRDTAIKQALREAGY
HHHHHHEEEEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCC
EAHSFKDTVIFEMKEVATADGRPYTVYTPYAKRWRSRLAAEPVTVQDMPRLATIPLPVSE
CHHCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCCHHCCCCEEECCCCCCC
PLPHLTDLLPDAPATLPRFPAGEAVALEALERFVRGPLASYAQGRDLMAVAGTSRLSPYL
CCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCHHCCHHH
RLGVLSPRQCVAAALAAPPGPGPESWIGELIWRDFYVQVLYHFPHALRGSFKPAYNRIDW
HCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PNDPVLFAAWQQGLTGYPIVDAAMRQLQREGWMHNRARMIVASFLTKDLLIDWRWGERHF
CCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCEE
MHLLIDGDPAANNGGWQWAAGTGTDAQPFFRIFNPVSQGQKFDPEGAYVRRYVPELVNVP
EEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
TRYIHEPHKMSLAEQRKAGVLIGRDYPAPIVDHATQRTKALELYRAATRHNDSSAEQYA
HHHCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]