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The map label for this gene is liaR [H]

Identifier: 222526915

GI number: 222526915

Start: 4605991

End: 4606620

Strand: Direct

Name: liaR [H]

Synonym: Chy400_3693

Alternate gene names: 222526915

Gene position: 4605991-4606620 (Clockwise)

Preceding gene: 222526914

Following gene: 222526922

Centisome position: 87.42

GC content: 56.35

Gene sequence:

>630_bases
ATGAGTCCGATTCGCGTTGCCCTGGTTGATGATCACAGCATTGTTCGGCGTGGACTGAGTGCGTATCTTGCCGCTCAACC
CGATATTGTGGTTGTGGGTGAGGCGGCGAATGGTGAGAGTGCCCTGCAGATGGCATCGACCTGGCAGGCGGATGTGATTG
TGATGGATGTACTCATGCCGGGTGGAATCGATGGGATCGAGACTACCCGGCAGCTCAAGCGCCTGATTCCACACACGCAC
ATTATCATTCTGAGCGGGTATGCCGATGATGCGCGGGTGATTGGTGCCTTGCGCGCCGGTGCAATCACGTACATCGAAAA
AGAGAGTCAACCAGAGCAGCTTCTCGAAGCGATTCGGGGTGCGATGGCGGGGAAAGCAGTGCTGGCACCGGCATTGATGC
AACGGATTCTCGAAGCTCAAACGTCCAAAGCCGCTGATACATTGACCGAACGCGAGCGCGAGGTGTTGAAACTGGTGGCC
GAAGGTCTCACCAATGCCGAAATCGCGAGTCGGCTGACCGTAAGTGAAGAGACAGTCAAAACCCACGTCGCCAGCATTCT
GCGCAAACTCGGTCTGGCTCATCGCACGCAAGCAGCGATCTATGCGCTACGGCATGGATTGGTGAGTTGA

Upstream 100 bases:

>100_bases
CGTAGTCTGACCTACGGTGTCCCACAACGGATGACCCCTCAGCCACAGCAGTCGTCGGTGGGTTGATAGCCGGTTCTGGC
AGGTAACAGGGAGAGACCAG

Downstream 100 bases:

>100_bases
TGGATACCGGTGGTGGTACGCAGCGGTAGGGATACGGCATGCCGTGCCCCTGCCGCATCAATGCGCTCCATCGTAGCGGA
AATGTAGTCCAGCCTGAATC

Product: LuxR family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSPIRVALVDDHSIVRRGLSAYLAAQPDIVVVGEAANGESALQMASTWQADVIVMDVLMPGGIDGIETTRQLKRLIPHTH
IIILSGYADDARVIGALRAGAITYIEKESQPEQLLEAIRGAMAGKAVLAPALMQRILEAQTSKAADTLTEREREVLKLVA
EGLTNAEIASRLTVSEETVKTHVASILRKLGLAHRTQAAIYALRHGLVS

Sequences:

>Translated_209_residues
MSPIRVALVDDHSIVRRGLSAYLAAQPDIVVVGEAANGESALQMASTWQADVIVMDVLMPGGIDGIETTRQLKRLIPHTH
IIILSGYADDARVIGALRAGAITYIEKESQPEQLLEAIRGAMAGKAVLAPALMQRILEAQTSKAADTLTEREREVLKLVA
EGLTNAEIASRLTVSEETVKTHVASILRKLGLAHRTQAAIYALRHGLVS
>Mature_208_residues
SPIRVALVDDHSIVRRGLSAYLAAQPDIVVVGEAANGESALQMASTWQADVIVMDVLMPGGIDGIETTRQLKRLIPHTHI
IILSGYADDARVIGALRAGAITYIEKESQPEQLLEAIRGAMAGKAVLAPALMQRILEAQTSKAADTLTEREREVLKLVAE
GLTNAEIASRLTVSEETVKTHVASILRKLGLAHRTQAAIYALRHGLVS

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=208, Percent_Identity=39.4230769230769, Blast_Score=133, Evalue=7e-33,
Organism=Escherichia coli, GI1787473, Length=205, Percent_Identity=39.5121951219512, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=32.7014218009479, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=35.2040816326531, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786747, Length=211, Percent_Identity=25.5924170616114, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1788712, Length=200, Percent_Identity=26.5, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788546, Length=198, Percent_Identity=29.7979797979798, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22443; Mature: 22312

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIRVALVDDHSIVRRGLSAYLAAQPDIVVVGEAANGESALQMASTWQADVIVMDVLMP
CCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHHHHEEHHCC
GGIDGIETTRQLKRLIPHTHIIILSGYADDARVIGALRAGAITYIEKESQPEQLLEAIRG
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
AMAGKAVLAPALMQRILEAQTSKAADTLTEREREVLKLVAEGLTNAEIASRLTVSEETVK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
THVASILRKLGLAHRTQAAIYALRHGLVS
HHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPIRVALVDDHSIVRRGLSAYLAAQPDIVVVGEAANGESALQMASTWQADVIVMDVLMP
CCEEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHHHHEEHHCC
GGIDGIETTRQLKRLIPHTHIIILSGYADDARVIGALRAGAITYIEKESQPEQLLEAIRG
CCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHH
AMAGKAVLAPALMQRILEAQTSKAADTLTEREREVLKLVAEGLTNAEIASRLTVSEETVK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
THVASILRKLGLAHRTQAAIYALRHGLVS
HHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]