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The map label for this gene is ppx [H]

Identifier: 222526908

GI number: 222526908

Start: 4597502

End: 4599007

Strand: Reverse

Name: ppx [H]

Synonym: Chy400_3686

Alternate gene names: 222526908

Gene position: 4599007-4597502 (Counterclockwise)

Preceding gene: 222526909

Following gene: 222526907

Centisome position: 87.29

GC content: 59.76

Gene sequence:

>1506_bases
ATGCAAAAACTCGGTGTGATCGATCTCGGTTCCAATACCACTCGTCTGATTGTGATGGCGTATGAACCGGGACACTGTTT
TCGCCTGACCGACGAAGTGAGCGAAACTGTCCGTCTGGCCGAGGGCATTGGCCCCTCTCGCATGCTGCAACCGGCACCGA
TTCGGCGTGCCGTTGAAGCCCTGCGCATGTTCTATTCACTCTGCATCTCGACCGGCGTCAATGAGGTGATTGCGGTCGGC
ACCAGTGCCATCCGCGAAGCAGCGAATCAGGCTGATTTCTGGGCAGCGTTGCGCACGGCAACCCCGCTCGATCTGCGCAT
CATCTCTGCCGAGGACGAGGCGTATTTCGGCTACCTCGGTGCAATCAATGCCTTGCCGATCCAGCACGGTGCGCTCTTTG
ATACTGGTGGTGGTTCGACCCAGGTGATGGCGGTGGCTGACCGGAAACTGACGCGGAGCTTTTCGGTGCAGGCCGGTGTG
GTGCGCTTCACCGAGCAGTTTGTGCAGAGCGATCCGATCAGTCGCACCGATCTGCGGCGGCTGCGTGCGGCTGCGCGTGA
TGCCTTTGCTCCCCTTGACTGGCTGCCCGACCTGAGCAATGGCCGTTTAGGTGGGATTGGTGGTACGGTACGCCAGTTGG
CCCGCATCGATCAGAAACAACGGGCCTACCCGCTTGAACGTGTCCACGGCTATCAGCTCACCCGCGAGGCGGTAGAGCGC
ATTACCGAAGAACTCGCCCGCCGTTCCCGTCGCGAACGATTGCAAATTCCCGGTATGAAAGAGGAACGGGCCGATGTAAC
GCTGGCCGGCGCCGTGATTATCGCTGCCCTGATGGATCGCGGCAATTTCCCGTACCTGCTGGTCAGCGGTCAAGGCGTGC
GCGAAGGCATCTTCTACCAGCACTTTCTGGCCGATCAACCGCAGCCCCTGCTGAACGAACCACGACAGTTCAGTGTACTC
AACCTGGCCCGGCTGACCCACTACGAACCGGAACACTGTGCTCGCGTCGCTCACCTCAGTCTGTCGCTGTTCGACCAGTT
GGCACCCCTGCACGGCTATGGCGCCTGGGAACGCGAACTACTCGGCTACGCTGCGATCCTGCACGACATTGGCATTAGCG
TCGGCTACTACGACCATCACAAACACGGCGAGTATCTCATTCACAATGCGACCCTGCTCGGCTTCAGCCATCGCGAAATT
GTGATCATCGCCAGCCTGGTACGCAACCATCGCAAAGGATGGGGCGATCTGACCGCCTACTCCCCCATTCTCGCCACCGA
CGACGAAACACGCATCGCCCGTCTGAGCGCAATGCTCCGCCTTGCCGAATACCTGGAACGGAGCAAGAGCCAGATCGTGC
GTGACGTGCGCGTCCAGATCGGCGACGAGATCGTGATTGATGTCATTGCCGATGGTGATGCCCACGTCGAAATTTGGGAA
GCCTCACGCCGCAGCGGTCTGTTAAAACGCAGCTTTGGCCGCGAGGTGCAGATCAGAGCAACATGA

Upstream 100 bases:

>100_bases
CTTGTTTCGTATCTCGCTGGTGATGCTCCCCTTGCCCTTTACTACCAGCAGTTTCGTGTATACTTATCCTTGATATGGCA
ACACTCACAGGATCAATCCT

Downstream 100 bases:

>100_bases
GCCTGTGCTATAATGCCACATAGCAAGAAGTGAGGGATAGGCTACACGCATGCGGTGCCCGTTTTGCCATTTTGTCGAGA
CCGATGTTATCGATACGCGC

Product: Ppx/GppA phosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MQKLGVIDLGSNTTRLIVMAYEPGHCFRLTDEVSETVRLAEGIGPSRMLQPAPIRRAVEALRMFYSLCISTGVNEVIAVG
TSAIREAANQADFWAALRTATPLDLRIISAEDEAYFGYLGAINALPIQHGALFDTGGGSTQVMAVADRKLTRSFSVQAGV
VRFTEQFVQSDPISRTDLRRLRAAARDAFAPLDWLPDLSNGRLGGIGGTVRQLARIDQKQRAYPLERVHGYQLTREAVER
ITEELARRSRRERLQIPGMKEERADVTLAGAVIIAALMDRGNFPYLLVSGQGVREGIFYQHFLADQPQPLLNEPRQFSVL
NLARLTHYEPEHCARVAHLSLSLFDQLAPLHGYGAWERELLGYAAILHDIGISVGYYDHHKHGEYLIHNATLLGFSHREI
VIIASLVRNHRKGWGDLTAYSPILATDDETRIARLSAMLRLAEYLERSKSQIVRDVRVQIGDEIVIDVIADGDAHVEIWE
ASRRSGLLKRSFGREVQIRAT

Sequences:

>Translated_501_residues
MQKLGVIDLGSNTTRLIVMAYEPGHCFRLTDEVSETVRLAEGIGPSRMLQPAPIRRAVEALRMFYSLCISTGVNEVIAVG
TSAIREAANQADFWAALRTATPLDLRIISAEDEAYFGYLGAINALPIQHGALFDTGGGSTQVMAVADRKLTRSFSVQAGV
VRFTEQFVQSDPISRTDLRRLRAAARDAFAPLDWLPDLSNGRLGGIGGTVRQLARIDQKQRAYPLERVHGYQLTREAVER
ITEELARRSRRERLQIPGMKEERADVTLAGAVIIAALMDRGNFPYLLVSGQGVREGIFYQHFLADQPQPLLNEPRQFSVL
NLARLTHYEPEHCARVAHLSLSLFDQLAPLHGYGAWERELLGYAAILHDIGISVGYYDHHKHGEYLIHNATLLGFSHREI
VIIASLVRNHRKGWGDLTAYSPILATDDETRIARLSAMLRLAEYLERSKSQIVRDVRVQIGDEIVIDVIADGDAHVEIWE
ASRRSGLLKRSFGREVQIRAT
>Mature_501_residues
MQKLGVIDLGSNTTRLIVMAYEPGHCFRLTDEVSETVRLAEGIGPSRMLQPAPIRRAVEALRMFYSLCISTGVNEVIAVG
TSAIREAANQADFWAALRTATPLDLRIISAEDEAYFGYLGAINALPIQHGALFDTGGGSTQVMAVADRKLTRSFSVQAGV
VRFTEQFVQSDPISRTDLRRLRAAARDAFAPLDWLPDLSNGRLGGIGGTVRQLARIDQKQRAYPLERVHGYQLTREAVER
ITEELARRSRRERLQIPGMKEERADVTLAGAVIIAALMDRGNFPYLLVSGQGVREGIFYQHFLADQPQPLLNEPRQFSVL
NLARLTHYEPEHCARVAHLSLSLFDQLAPLHGYGAWERELLGYAAILHDIGISVGYYDHHKHGEYLIHNATLLGFSHREI
VIIASLVRNHRKGWGDLTAYSPILATDDETRIARLSAMLRLAEYLERSKSQIVRDVRVQIGDEIVIDVIADGDAHVEIWE
ASRRSGLLKRSFGREVQIRAT

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=421, Percent_Identity=26.3657957244656, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI48994966, Length=472, Percent_Identity=27.1186440677966, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 55932; Mature: 55932

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLGVIDLGSNTTRLIVMAYEPGHCFRLTDEVSETVRLAEGIGPSRMLQPAPIRRAVEA
CCCCEEEEECCCCEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHH
LRMFYSLCISTGVNEVIAVGTSAIREAANQADFWAALRTATPLDLRIISAEDEAYFGYLG
HHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHH
AINALPIQHGALFDTGGGSTQVMAVADRKLTRSFSVQAGVVRFTEQFVQSDPISRTDLRR
CCCCCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LRAAARDAFAPLDWLPDLSNGRLGGIGGTVRQLARIDQKQRAYPLERVHGYQLTREAVER
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ITEELARRSRRERLQIPGMKEERADVTLAGAVIIAALMDRGNFPYLLVSGQGVREGIFYQ
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
HFLADQPQPLLNEPRQFSVLNLARLTHYEPEHCARVAHLSLSLFDQLAPLHGYGAWEREL
HHHCCCCCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LGYAAILHDIGISVGYYDHHKHGEYLIHNATLLGFSHREIVIIASLVRNHRKGWGDLTAY
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
SPILATDDETRIARLSAMLRLAEYLERSKSQIVRDVRVQIGDEIVIDVIADGDAHVEIWE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEE
ASRRSGLLKRSFGREVQIRAT
CHHHCCHHHHHCCCEEEEEEC
>Mature Secondary Structure
MQKLGVIDLGSNTTRLIVMAYEPGHCFRLTDEVSETVRLAEGIGPSRMLQPAPIRRAVEA
CCCCEEEEECCCCEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHH
LRMFYSLCISTGVNEVIAVGTSAIREAANQADFWAALRTATPLDLRIISAEDEAYFGYLG
HHHHHHHHHHHCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHH
AINALPIQHGALFDTGGGSTQVMAVADRKLTRSFSVQAGVVRFTEQFVQSDPISRTDLRR
CCCCCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LRAAARDAFAPLDWLPDLSNGRLGGIGGTVRQLARIDQKQRAYPLERVHGYQLTREAVER
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ITEELARRSRRERLQIPGMKEERADVTLAGAVIIAALMDRGNFPYLLVSGQGVREGIFYQ
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHH
HFLADQPQPLLNEPRQFSVLNLARLTHYEPEHCARVAHLSLSLFDQLAPLHGYGAWEREL
HHHCCCCCHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LGYAAILHDIGISVGYYDHHKHGEYLIHNATLLGFSHREIVIIASLVRNHRKGWGDLTAY
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
SPILATDDETRIARLSAMLRLAEYLERSKSQIVRDVRVQIGDEIVIDVIADGDAHVEIWE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEE
ASRRSGLLKRSFGREVQIRAT
CHHHCCHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]