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The map label for this gene is ntcA [H]

Identifier: 222526891

GI number: 222526891

Start: 4576384

End: 4577052

Strand: Direct

Name: ntcA [H]

Synonym: Chy400_3669

Alternate gene names: 222526891

Gene position: 4576384-4577052 (Clockwise)

Preceding gene: 222526890

Following gene: 222526912

Centisome position: 86.86

GC content: 58.59

Gene sequence:

>669_bases
ATGAGTGACACGCGCGATGTCCTCTCGCTCTTTCACGATCTGGGGCCGGCGGCACGGGCAGACTTGACGGCGCTGTTGCA
GGTCGCCTCGTATCCGTCCGGGCATATCTTTCATCTTCCGAACGAAACCGGTACGCACGTGTTTTTCTTACAGCAGGGGC
GGGTGCGGATTTACAAGGTGTCGCCTGAAGGGAGAGCGCTTACGCTGCTGGTATTGCATCCACCCGGTCTGTTTGGCGAA
ATGGCCCTGATCGGGGCCGGGATGCACGACACGTGTGCCGAGGCGATGACCGATTGTACCGTGGCCCGCATCGAGCGGGA
AGCACTGCGCAATTTTCTCGGTCGCTACCCTGCCGCAGCAATTGATCTGATGGAATTGATGGGGTCGCGCCTGCGCACAA
TGGAGCAACGCCTGGCCGATATTGCCTTCAAAAGCGTACCCCAACGTCTGGCCGCACTCTTACTTGATTTGGCCGGTGAT
CCGACGGATGATACTGCACCGATGAAGATTGTTCGCTATACCCACCAGCAACTGGCCGAGATGATCGGTGCCTATCGAGA
GACGGTCACCAAAGCCCTGGGTGATATGCGCGAAGCGAATCTGATCCAGGTCATCGACGATGCCATCATTCTGACCGACC
CGGCTCAGTTGCGTCGGCTGGCGTCGTGA

Upstream 100 bases:

>100_bases
CATTAGCAGCACTCCAACGCGGTGCCAGCAGTGTGCGCATCGTCAATCTGGCCGGTTTGCACGACGGTGGTACTCGTTTT
ACGCACGGAGAATTGTCTGA

Downstream 100 bases:

>100_bases
GCCACCGCTCGCGCTTTGGTGTAAGGGTTCAACCCGCACGAGTCGGGGGTGGCACCTCTAGCCCCGCAGGGGCTTGCCCG
TCGTGAGGCAGGGCTTTAGC

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSDTRDVLSLFHDLGPAARADLTALLQVASYPSGHIFHLPNETGTHVFFLQQGRVRIYKVSPEGRALTLLVLHPPGLFGE
MALIGAGMHDTCAEAMTDCTVARIEREALRNFLGRYPAAAIDLMELMGSRLRTMEQRLADIAFKSVPQRLAALLLDLAGD
PTDDTAPMKIVRYTHQQLAEMIGAYRETVTKALGDMREANLIQVIDDAIILTDPAQLRRLAS

Sequences:

>Translated_222_residues
MSDTRDVLSLFHDLGPAARADLTALLQVASYPSGHIFHLPNETGTHVFFLQQGRVRIYKVSPEGRALTLLVLHPPGLFGE
MALIGAGMHDTCAEAMTDCTVARIEREALRNFLGRYPAAAIDLMELMGSRLRTMEQRLADIAFKSVPQRLAALLLDLAGD
PTDDTAPMKIVRYTHQQLAEMIGAYRETVTKALGDMREANLIQVIDDAIILTDPAQLRRLAS
>Mature_221_residues
SDTRDVLSLFHDLGPAARADLTALLQVASYPSGHIFHLPNETGTHVFFLQQGRVRIYKVSPEGRALTLLVLHPPGLFGEM
ALIGAGMHDTCAEAMTDCTVARIEREALRNFLGRYPAAAIDLMELMGSRLRTMEQRLADIAFKSVPQRLAALLLDLAGDP
TDDTAPMKIVRYTHQQLAEMIGAYRETVTKALGDMREANLIQVIDDAIILTDPAQLRRLAS

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=187, Percent_Identity=27.807486631016, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24451; Mature: 24319

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTRDVLSLFHDLGPAARADLTALLQVASYPSGHIFHLPNETGTHVFFLQQGRVRIYKV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCEEEEEE
SPEGRALTLLVLHPPGLFGEMALIGAGMHDTCAEAMTDCTVARIEREALRNFLGRYPAAA
CCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IDLMELMGSRLRTMEQRLADIAFKSVPQRLAALLLDLAGDPTDDTAPMKIVRYTHQQLAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MIGAYRETVTKALGDMREANLIQVIDDAIILTDPAQLRRLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCC
>Mature Secondary Structure 
SDTRDVLSLFHDLGPAARADLTALLQVASYPSGHIFHLPNETGTHVFFLQQGRVRIYKV
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECCCEEEEEE
SPEGRALTLLVLHPPGLFGEMALIGAGMHDTCAEAMTDCTVARIEREALRNFLGRYPAAA
CCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IDLMELMGSRLRTMEQRLADIAFKSVPQRLAALLLDLAGDPTDDTAPMKIVRYTHQQLAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MIGAYRETVTKALGDMREANLIQVIDDAIILTDPAQLRRLAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630321 [H]