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The map label for this gene is 222526791

Identifier: 222526791

GI number: 222526791

Start: 4441365

End: 4442252

Strand: Direct

Name: 222526791

Synonym: Chy400_3565

Alternate gene names: NA

Gene position: 4441365-4442252 (Clockwise)

Preceding gene: 222526790

Following gene: 222526792

Centisome position: 84.29

GC content: 58.33

Gene sequence:

>888_bases
ATGACGCAACGAATTGTCAATATCGGTCTGGTGCAGATGCGCTGTACCGCCGATCCTGACTTCAATATGGCGACGGCGGA
AGCCGGGATTCGGGCTGCGGCGGCGCAGGGGGCGCAGATTGTGTGTCTGCCGGAGCTGTTTCGTTCGCTCTATTTCTGTC
AGAGCGAGAATCATGTTTTCTTCGCTCTGGCCGAGCCGGTGCCCGGCCCCAGTACCGAGCGGCTCAGTAAATTGGCTGCT
GAGTTGCAGGTGGTGATTGTGGCCAGTCTGTTCGAGAAACGGGCGGAAGGTCTCTACCACAATACGGCGGCGGTGATCGA
TGCTGATGGCCGGTATCTGGGGAAGTACCGTAAGATGCACATTCCCGATGATCCGCTCTTCTACGAGAAGTTTTATTTCA
CGCCGGGTGATCTGGGGTTCAAGGTGTTCAAAACACGCTACGCCCGGATCGGGGTGCTAATCTGCTGGGATCAGTGGTAC
CCCGAAGCGGCCCGGCTCACGGCGCTGCGCGGAGCCGATGTGCTCTGTTATCCGACGGCAATTGGCTGGCATCCCGCCGA
GAAGGCTGAGTACGGTGTAGCGCAACATCAGAGCTGGGAGATTATCCAGCGCTCACACGGGATTGCCAACGGTTGTTACG
TCGTGAGCATCAATCGTACCGGTCACGAAGGTGATCCTGCCGGTGGGATCGAGTTCTGGGGGCAGAGCTTTATCTCCGAT
CCTTCGGGAACGGTGATCGTGCGGGCGCCGGTTGATGAGCCGGCGGTGCTGGTCGCCCCCATCGATCTGGCAAAGATTGA
TGTGCAGCGTACTCACTGGCCATTTCTGCGCGACCGCCGGATTGATGCCTACGGTGAGATTACGCGCCGTTATCTTGATG
AGGAGTAA

Upstream 100 bases:

>100_bases
GTACGTCGCCGGTCGAGCTGCGGAAGCAGCGATCCGACGTGGCGAATTGCGCTGGATTAGTAATGGTGAGTGGTGATACC
ACATGACCCTGGAGAAGACC

Downstream 100 bases:

>100_bases
GCGTTATGGCAACCCCTGCCGAACTGGGTTACCGGATGCCCGCCGAATGGGAACCGCATCAGGCTACGTGGCTTTCGTGG
CCGCATAAAGAGGAGAGCTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTQRIVNIGLVQMRCTADPDFNMATAEAGIRAAAAQGAQIVCLPELFRSLYFCQSENHVFFALAEPVPGPSTERLSKLAA
ELQVVIVASLFEKRAEGLYHNTAAVIDADGRYLGKYRKMHIPDDPLFYEKFYFTPGDLGFKVFKTRYARIGVLICWDQWY
PEAARLTALRGADVLCYPTAIGWHPAEKAEYGVAQHQSWEIIQRSHGIANGCYVVSINRTGHEGDPAGGIEFWGQSFISD
PSGTVIVRAPVDEPAVLVAPIDLAKIDVQRTHWPFLRDRRIDAYGEITRRYLDEE

Sequences:

>Translated_295_residues
MTQRIVNIGLVQMRCTADPDFNMATAEAGIRAAAAQGAQIVCLPELFRSLYFCQSENHVFFALAEPVPGPSTERLSKLAA
ELQVVIVASLFEKRAEGLYHNTAAVIDADGRYLGKYRKMHIPDDPLFYEKFYFTPGDLGFKVFKTRYARIGVLICWDQWY
PEAARLTALRGADVLCYPTAIGWHPAEKAEYGVAQHQSWEIIQRSHGIANGCYVVSINRTGHEGDPAGGIEFWGQSFISD
PSGTVIVRAPVDEPAVLVAPIDLAKIDVQRTHWPFLRDRRIDAYGEITRRYLDEE
>Mature_294_residues
TQRIVNIGLVQMRCTADPDFNMATAEAGIRAAAAQGAQIVCLPELFRSLYFCQSENHVFFALAEPVPGPSTERLSKLAAE
LQVVIVASLFEKRAEGLYHNTAAVIDADGRYLGKYRKMHIPDDPLFYEKFYFTPGDLGFKVFKTRYARIGVLICWDQWYP
EAARLTALRGADVLCYPTAIGWHPAEKAEYGVAQHQSWEIIQRSHGIANGCYVVSINRTGHEGDPAGGIEFWGQSFISDP
SGTVIVRAPVDEPAVLVAPIDLAKIDVQRTHWPFLRDRRIDAYGEITRRYLDEE

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=285, Percent_Identity=34.0350877192982, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI7706509, Length=306, Percent_Identity=30.0653594771242, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI297632350, Length=290, Percent_Identity=27.9310344827586, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI297632348, Length=290, Percent_Identity=27.9310344827586, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI5031947, Length=290, Percent_Identity=27.9310344827586, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI297632346, Length=185, Percent_Identity=27.5675675675676, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17533173, Length=275, Percent_Identity=33.8181818181818, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17556280, Length=285, Percent_Identity=27.719298245614, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323383, Length=303, Percent_Identity=26.7326732673267, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6322335, Length=318, Percent_Identity=24.5283018867925, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21358471, Length=277, Percent_Identity=31.4079422382672, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355835, Length=292, Percent_Identity=27.7397260273973, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17933642, Length=283, Percent_Identity=25.4416961130742, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33053; Mature: 32922

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRIVNIGLVQMRCTADPDFNMATAEAGIRAAAAQGAQIVCLPELFRSLYFCQSENHVF
CCCEEEEEEEEEEEECCCCCCCEEHHHHCHHHHHCCCCEEEEHHHHHHHHHEECCCCCEE
FALAEPVPGPSTERLSKLAAELQVVIVASLFEKRAEGLYHNTAAVIDADGRYLGKYRKMH
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCEEECC
IPDDPLFYEKFYFTPGDLGFKVFKTRYARIGVLICWDQWYPEAARLTALRGADVLCYPTA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHEEEEEEECCCCCCHHHEEEECCCCEEEECCC
IGWHPAEKAEYGVAQHQSWEIIQRSHGIANGCYVVSINRTGHEGDPAGGIEFWGQSFISD
CCCCCCCHHHCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCC
PSGTVIVRAPVDEPAVLVAPIDLAKIDVQRTHWPFLRDRRIDAYGEITRRYLDEE
CCCEEEEECCCCCCEEEEEECCEEEEEECCCCCCCHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TQRIVNIGLVQMRCTADPDFNMATAEAGIRAAAAQGAQIVCLPELFRSLYFCQSENHVF
CCEEEEEEEEEEEECCCCCCCEEHHHHCHHHHHCCCCEEEEHHHHHHHHHEECCCCCEE
FALAEPVPGPSTERLSKLAAELQVVIVASLFEKRAEGLYHNTAAVIDADGRYLGKYRKMH
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEECCEEECC
IPDDPLFYEKFYFTPGDLGFKVFKTRYARIGVLICWDQWYPEAARLTALRGADVLCYPTA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHEEEEEEECCCCCCHHHEEEECCCCEEEECCC
IGWHPAEKAEYGVAQHQSWEIIQRSHGIANGCYVVSINRTGHEGDPAGGIEFWGQSFISD
CCCCCCCHHHCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCC
PSGTVIVRAPVDEPAVLVAPIDLAKIDVQRTHWPFLRDRRIDAYGEITRRYLDEE
CCCEEEEECCCCCCEEEEEECCEEEEEECCCCCCCHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]