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The map label for this gene is mdtG [H]

Identifier: 222526788

GI number: 222526788

Start: 4438661

End: 4439863

Strand: Reverse

Name: mdtG [H]

Synonym: Chy400_3562

Alternate gene names: 222526788

Gene position: 4439863-4438661 (Counterclockwise)

Preceding gene: 222526789

Following gene: 222526787

Centisome position: 84.26

GC content: 61.76

Gene sequence:

>1203_bases
ATGCAAACACGCTGGCAAACAACCCTCTGGATCATGTTCGTCGCCCAGTTTCTTTCGGCAATCGGGTTTTCGATCATCTT
TCCATTTCTGCCGTTGTACGTCGCCTATCTGGGCGTGGTGACCATCGGCGACGTTGCCCTCTGGTCAGGTCTGGTCTTCT
CACTGCAAGCCCTGACCATGGCAATTGCCGCACCGATCTGGGGATCACTGGCCGACCGGCTCGGCTACAAACTGATGGTC
GAACGGGCCATGTACGGCGGTGCCATTATCCTGCTGCTCATGGGTTTTGCCCGTTCCGCCGAAGAATTAACCCTGCTACG
CACCATCCAGGGCATGATCACCGGCACCATCTCGGCTGCCAATGCCCTGGTAGCAACCGTCACCCCCCGCGAACGCATGG
GCTTCGCGATGGGGACGCTCCAGATGGGCTTATGGAGTGGCACCGCAGCCGGCCCGCTAATCGGCGGCATCATGGCCGAA
ACCCTCGGCTTTCGGGCGACCTTCATCACCACCGCAGCACTCCTGCTCATCTCCGGCATCCTGGTCACGGTTGGGGTGCG
CGGCGTGCGCCAGACGGCCTCTGCCACCAAACGGCCGTCGTTGAGCATGTGGCAAAGCTGGTTGAGCATCTTACGCACAC
CCGGCATCAGACCGGCCTACAGCATGCGCTTCCTCAGCAGCCTGTCCCAAACGATCCTGCTGCCGTTCGCACCGCTATTC
ATTGCCAGCCTGCTCTCCCCAACCGATCCGGTAAGCGCCTACACCGGCCTGATAGTTGGCATTTCATCAGCCGCCGGCAC
AGCGACCGCCATCTGGCTGGGGCGCCTCGGTGATCGCATCGGGCATCAGCAGGTGTTAATGGTCAGCGCCCTGGTGGCGG
GAATCGCACTTCTGCCGCAAGGCATCGTCACCAATATCTGGCAGCTCCTGATCCTGCAAGCCCTGAGTGGTGCGGCAATC
GGCGGTATCACCCCCTCGCTCAGCGCACTCCTCGGTCGTTACACCGCCGAAGGGAATGAAGGCGCAGTATACGGCCTCGA
CAGCTCAATTGTCTCAGCAGCCCGCGCCGTCGCTCCCCTCATCGGTGCCATGGTGGTTACTCCTTTTGGGCTGGGGAGTG
CCTTCTTCATAAGCGGCTGTGCCTGCCTGATCATCGCCGCAGGTGCCGCCCGGCTACCTGCACTGGTGGTGGCACCCCGC
TGA

Upstream 100 bases:

>100_bases
GACTGGAATCCTTTGCCACCAGCGTATTGCCCAGCCTGGGACAGGCTTGACCGCATCAGCCCTGCCGTTATAACGAGAGA
GATATTCCCTGAATACGATC

Downstream 100 bases:

>100_bases
GCAGGTAACGTCGCGGCAAGCATTGGTGGCGTGGGGAGTGGGGAGTGGGGAGTGGGGAGTGGGGAGTGGGGAGTGGGGGG
AGCGTGCTCCCCATCCGGGA

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQTRWQTTLWIMFVAQFLSAIGFSIIFPFLPLYVAYLGVVTIGDVALWSGLVFSLQALTMAIAAPIWGSLADRLGYKLMV
ERAMYGGAIILLLMGFARSAEELTLLRTIQGMITGTISAANALVATVTPRERMGFAMGTLQMGLWSGTAAGPLIGGIMAE
TLGFRATFITTAALLLISGILVTVGVRGVRQTASATKRPSLSMWQSWLSILRTPGIRPAYSMRFLSSLSQTILLPFAPLF
IASLLSPTDPVSAYTGLIVGISSAAGTATAIWLGRLGDRIGHQQVLMVSALVAGIALLPQGIVTNIWQLLILQALSGAAI
GGITPSLSALLGRYTAEGNEGAVYGLDSSIVSAARAVAPLIGAMVVTPFGLGSAFFISGCACLIIAAGAARLPALVVAPR

Sequences:

>Translated_400_residues
MQTRWQTTLWIMFVAQFLSAIGFSIIFPFLPLYVAYLGVVTIGDVALWSGLVFSLQALTMAIAAPIWGSLADRLGYKLMV
ERAMYGGAIILLLMGFARSAEELTLLRTIQGMITGTISAANALVATVTPRERMGFAMGTLQMGLWSGTAAGPLIGGIMAE
TLGFRATFITTAALLLISGILVTVGVRGVRQTASATKRPSLSMWQSWLSILRTPGIRPAYSMRFLSSLSQTILLPFAPLF
IASLLSPTDPVSAYTGLIVGISSAAGTATAIWLGRLGDRIGHQQVLMVSALVAGIALLPQGIVTNIWQLLILQALSGAAI
GGITPSLSALLGRYTAEGNEGAVYGLDSSIVSAARAVAPLIGAMVVTPFGLGSAFFISGCACLIIAAGAARLPALVVAPR
>Mature_400_residues
MQTRWQTTLWIMFVAQFLSAIGFSIIFPFLPLYVAYLGVVTIGDVALWSGLVFSLQALTMAIAAPIWGSLADRLGYKLMV
ERAMYGGAIILLLMGFARSAEELTLLRTIQGMITGTISAANALVATVTPRERMGFAMGTLQMGLWSGTAAGPLIGGIMAE
TLGFRATFITTAALLLISGILVTVGVRGVRQTASATKRPSLSMWQSWLSILRTPGIRPAYSMRFLSSLSQTILLPFAPLF
IASLLSPTDPVSAYTGLIVGISSAAGTATAIWLGRLGDRIGHQQVLMVSALVAGIALLPQGIVTNIWQLLILQALSGAAI
GGITPSLSALLGRYTAEGNEGAVYGLDSSIVSAARAVAPLIGAMVVTPFGLGSAFFISGCACLIIAAGAARLPALVVAPR

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=383, Percent_Identity=32.3759791122715, Blast_Score=188, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6319871, Length=155, Percent_Identity=25.1612903225806, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62471713, Length=348, Percent_Identity=28.735632183908, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI62471717, Length=348, Percent_Identity=28.735632183908, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI62471715, Length=302, Percent_Identity=30.1324503311258, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41764; Mature: 41764

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRWQTTLWIMFVAQFLSAIGFSIIFPFLPLYVAYLGVVTIGDVALWSGLVFSLQALTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAAPIWGSLADRLGYKLMVERAMYGGAIILLLMGFARSAEELTLLRTIQGMITGTISAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NALVATVTPRERMGFAMGTLQMGLWSGTAAGPLIGGIMAETLGFRATFITTAALLLISGI
HHEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTVGVRGVRQTASATKRPSLSMWQSWLSILRTPGIRPAYSMRFLSSLSQTILLPFAPLF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IASLLSPTDPVSAYTGLIVGISSAAGTATAIWLGRLGDRIGHQQVLMVSALVAGIALLPQ
HHHHCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
GIVTNIWQLLILQALSGAAIGGITPSLSALLGRYTAEGNEGAVYGLDSSIVSAARAVAPL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHH
IGAMVVTPFGLGSAFFISGCACLIIAAGAARLPALVVAPR
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCEEEECCC
>Mature Secondary Structure
MQTRWQTTLWIMFVAQFLSAIGFSIIFPFLPLYVAYLGVVTIGDVALWSGLVFSLQALTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIAAPIWGSLADRLGYKLMVERAMYGGAIILLLMGFARSAEELTLLRTIQGMITGTISAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
NALVATVTPRERMGFAMGTLQMGLWSGTAAGPLIGGIMAETLGFRATFITTAALLLISGI
HHEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTVGVRGVRQTASATKRPSLSMWQSWLSILRTPGIRPAYSMRFLSSLSQTILLPFAPLF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IASLLSPTDPVSAYTGLIVGISSAAGTATAIWLGRLGDRIGHQQVLMVSALVAGIALLPQ
HHHHCCCCCCHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
GIVTNIWQLLILQALSGAAIGGITPSLSALLGRYTAEGNEGAVYGLDSSIVSAARAVAPL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHH
IGAMVVTPFGLGSAFFISGCACLIIAAGAARLPALVVAPR
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA