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The map label for this gene is galE [C]

Identifier: 222526739

GI number: 222526739

Start: 4371576

End: 4372538

Strand: Reverse

Name: galE [C]

Synonym: Chy400_3513

Alternate gene names: 222526739

Gene position: 4372538-4371576 (Counterclockwise)

Preceding gene: 222526740

Following gene: 222526738

Centisome position: 82.99

GC content: 52.65

Gene sequence:

>963_bases
ATGACCTATCTTGTTACCGGTGCAGCCGGTTTTATTGGAAGTCATCTGGTGGATCGTCTGCTGGCACGGGGTGAACAGGT
AATCGGTTTTGACAATTTTGTCGATTATTACAGTCCAGATCGTAAACGGCGCAACCTCGCGCAGGCGATGCAACAGCCGG
GGTTTACATTGATAGAGGGTGATATTCGTGATCCCGACACGGTAGCACACATTTTCACTCGCTACCGTCCACGTTTCGTT
GCCCACCTGGCTGCGATGCCCGGCCCACGCCCATCGATTGCCAATCCGCAGCTCTATGAAGCCGTCAATGTTGGTGGGAG
TCTGGTAATTCTCGATTACGCCCGGCGTAGCGAAGTAGAAAATCTGGTACTGGCATCTACCTCATCGGTATACGGAAAAA
CAAACCGGGTGCCATTCCGCGAAGATGATAACACTGACCGACCGTTATCACCTTATGCAGCAACCAAAAAAGCGGCTGAA
GTTCTGGCATATACTTTTCACAGCCTGTACGGCATTCCCACCAGTGTGGTGCGCTTCTTTACCGTCTACGGCCCACGTGG
ACGGCCCGATATGACACCATACCTGTTTGTGGAACGAATGGTGCGTGGCCAGCCGATTACCCTCTTCAACGGTGGCGAAA
ATCTGTTCCGCGATTACACCTATATCGATGATATTGTCAGCGGGGTGATTAACGCCCTTGATCGACCGCATCCCTACGAA
ATCTTCAATCTTGGTCATTCGCAACCGGTCGAGCTGCGTCGCTTTGTCAACCTCCTGGAACAGATTACCGGCTACCCGGC
CCAGATCGAGATCAAGCCATTGCCGGCCACAGAGCCACCGATCACCTATGCAGACACAACCAAAGCCGGTCAATTGCTCG
ATTTCGCACCGCGTGTGGCCATTGAAGAAGGACTTGCCCGCTTCTGGGCATGGTATTGTGACGAACATCAGCCGCAACAT
TAA

Upstream 100 bases:

>100_bases
ACAATCTATTTGAAAATCTGCACGTCTTGAACATGGGTGTTCAATTTGGCGTCATTATTGCCATGCTGGCAACCATTGAC
GCCGAGCGCAAAGAGGAGTT

Downstream 100 bases:

>100_bases
CACAGGTTGCGTATACGCTATCTGCCGGGTACGATAAGATCAGAAGTCATACAGATCAACTGCCAGGGGCGGGAAGGTAT
ATCAGGTTATGAGCGATCAG

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTYLVTGAAGFIGSHLVDRLLARGEQVIGFDNFVDYYSPDRKRRNLAQAMQQPGFTLIEGDIRDPDTVAHIFTRYRPRFV
AHLAAMPGPRPSIANPQLYEAVNVGGSLVILDYARRSEVENLVLASTSSVYGKTNRVPFREDDNTDRPLSPYAATKKAAE
VLAYTFHSLYGIPTSVVRFFTVYGPRGRPDMTPYLFVERMVRGQPITLFNGGENLFRDYTYIDDIVSGVINALDRPHPYE
IFNLGHSQPVELRRFVNLLEQITGYPAQIEIKPLPATEPPITYADTTKAGQLLDFAPRVAIEEGLARFWAWYCDEHQPQH

Sequences:

>Translated_320_residues
MTYLVTGAAGFIGSHLVDRLLARGEQVIGFDNFVDYYSPDRKRRNLAQAMQQPGFTLIEGDIRDPDTVAHIFTRYRPRFV
AHLAAMPGPRPSIANPQLYEAVNVGGSLVILDYARRSEVENLVLASTSSVYGKTNRVPFREDDNTDRPLSPYAATKKAAE
VLAYTFHSLYGIPTSVVRFFTVYGPRGRPDMTPYLFVERMVRGQPITLFNGGENLFRDYTYIDDIVSGVINALDRPHPYE
IFNLGHSQPVELRRFVNLLEQITGYPAQIEIKPLPATEPPITYADTTKAGQLLDFAPRVAIEEGLARFWAWYCDEHQPQH
>Mature_319_residues
TYLVTGAAGFIGSHLVDRLLARGEQVIGFDNFVDYYSPDRKRRNLAQAMQQPGFTLIEGDIRDPDTVAHIFTRYRPRFVA
HLAAMPGPRPSIANPQLYEAVNVGGSLVILDYARRSEVENLVLASTSSVYGKTNRVPFREDDNTDRPLSPYAATKKAAEV
LAYTFHSLYGIPTSVVRFFTVYGPRGRPDMTPYLFVERMVRGQPITLFNGGENLFRDYTYIDDIVSGVINALDRPHPYEI
FNLGHSQPVELRRFVNLLEQITGYPAQIEIKPLPATEPPITYADTTKAGQLLDFAPRVAIEEGLARFWAWYCDEHQPQH

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=324, Percent_Identity=29.6296296296296, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI7657641, Length=319, Percent_Identity=26.6457680250784, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI56237023, Length=333, Percent_Identity=25.2252252252252, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI56118217, Length=333, Percent_Identity=25.2252252252252, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI189083684, Length=333, Percent_Identity=25.2252252252252, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4504031, Length=329, Percent_Identity=24.6200607902736, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786974, Length=334, Percent_Identity=26.0479041916168, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI48994969, Length=331, Percent_Identity=26.5861027190332, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788366, Length=274, Percent_Identity=25.9124087591241, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=343, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71982035, Length=341, Percent_Identity=28.4457478005865, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17539532, Length=321, Percent_Identity=26.791277258567, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=23.4042553191489, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=24.4047619047619, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=264, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=264, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=264, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=264, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=264, Percent_Identity=25.7575757575758, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=339, Percent_Identity=23.598820058997, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=30.188679245283, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19923002, Length=337, Percent_Identity=26.7062314540059, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 36129; Mature: 35998

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLVTGAAGFIGSHLVDRLLARGEQVIGFDNFVDYYSPDRKRRNLAQAMQQPGFTLIEG
CEEEEECHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEC
DIRDPDTVAHIFTRYRPRFVAHLAAMPGPRPSIANPQLYEAVNVGGSLVILDYARRSEVE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECHHHHHHH
NLVLASTSSVYGKTNRVPFREDDNTDRPLSPYAATKKAAEVLAYTFHSLYGIPTSVVRFF
HEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TVYGPRGRPDMTPYLFVERMVRGQPITLFNGGENLFRDYTYIDDIVSGVINALDRPHPYE
HHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IFNLGHSQPVELRRFVNLLEQITGYPAQIEIKPLPATEPPITYADTTKAGQLLDFAPRVA
EEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHH
IEEGLARFWAWYCDEHQPQH
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TYLVTGAAGFIGSHLVDRLLARGEQVIGFDNFVDYYSPDRKRRNLAQAMQQPGFTLIEG
EEEEECHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEC
DIRDPDTVAHIFTRYRPRFVAHLAAMPGPRPSIANPQLYEAVNVGGSLVILDYARRSEVE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEEEECHHHHHHH
NLVLASTSSVYGKTNRVPFREDDNTDRPLSPYAATKKAAEVLAYTFHSLYGIPTSVVRFF
HEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TVYGPRGRPDMTPYLFVERMVRGQPITLFNGGENLFRDYTYIDDIVSGVINALDRPHPYE
HHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IFNLGHSQPVELRRFVNLLEQITGYPAQIEIKPLPATEPPITYADTTKAGQLLDFAPRVA
EEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHH
IEEGLARFWAWYCDEHQPQH
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]