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The map label for this gene is rutD [H]

Identifier: 222526647

GI number: 222526647

Start: 4256595

End: 4257362

Strand: Reverse

Name: rutD [H]

Synonym: Chy400_3416

Alternate gene names: 222526647

Gene position: 4257362-4256595 (Counterclockwise)

Preceding gene: 222526648

Following gene: 222526646

Centisome position: 80.8

GC content: 62.37

Gene sequence:

>768_bases
ATGCCACTCGTGACGACAACAGCCGGCGATCTGTTTGTGATGCGGCGAGGCACGAACGGGGTGCCGTTGATTTTTGTTCA
CGGTGCCGGGGGGAGTGGTCGCCATTGGGGCCGGCTATTTGCCCTGTTGCCGCCAACAGTTCAGTTTATCGCCGTTGACC
TGCCGGGTCACGGTCGGTCACCACTGGCCGGCCCGATTACGATTGAACGCTATGCAGCGCAGATCGCTGCGCTTCACCAG
GCACTTGCCCTACCACCAGCCCTGATTATCGGTCATTCGATGGGCGGAGCGATTGCCTTGCAGCTTGCAATCACGACGCC
GCAGATCGTTGCCGGTCTGGGCCTGTTCGGCAGTGCTGCCCGTTTGCGAGTTGCTCCTGCGCTGCTCGATGGGTTGGCCG
GCGACGAGGCTACCCGACAGGCAACCATCACCACACTGGTTACCTGGCTCTTTAGCCCTGCCGCCGACCCAACGCTTTTT
GCTGAAGCCAGGGCCGAATACGCCGCGCTTGACCCGGCGATTCTGCTGGCCGATTTTCAGGCGTGCGATGGGTTCGACGT
GCGGAACCGGCTCGGTGACATCCACTGCCCGGCGCTGGTGATCACCGGCAGCGACGACCGCCTTACCCCGCCCAAACTCG
GCGCCGAACTGGCGAGCGGGCTTGGCGTACCACACCATCTCCTGGCCAATGTCGGCCATATGCCAATGTTGGAAGCGCCC
GATCAGCTAAGCGAGCTGCTGCGTGAATGGCTGACAACGCTTGCTTGA

Upstream 100 bases:

>100_bases
GTTATAACCGGGTGCGACTGGCGCTCACCACCCACGATGCCGGCGGGTTGACCGAGAAGGACTTTGCGTTGGCCGCCGCG
ATTGATGAGATTATGGGCTG

Downstream 100 bases:

>100_bases
GGTGAACAGCTCGCGTAGAATGGGATATAGCGGTCAGCGCAAGCGCAAATACGAATGAAGGAGGCGATCATGGAGTTCCA
GCTTACCGAAGATCAGCAGA

Product: alpha/beta hydrolase fold protein

Products: alcohol; carboxylate

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MPLVTTTAGDLFVMRRGTNGVPLIFVHGAGGSGRHWGRLFALLPPTVQFIAVDLPGHGRSPLAGPITIERYAAQIAALHQ
ALALPPALIIGHSMGGAIALQLAITTPQIVAGLGLFGSAARLRVAPALLDGLAGDEATRQATITTLVTWLFSPAADPTLF
AEARAEYAALDPAILLADFQACDGFDVRNRLGDIHCPALVITGSDDRLTPPKLGAELASGLGVPHHLLANVGHMPMLEAP
DQLSELLREWLTTLA

Sequences:

>Translated_255_residues
MPLVTTTAGDLFVMRRGTNGVPLIFVHGAGGSGRHWGRLFALLPPTVQFIAVDLPGHGRSPLAGPITIERYAAQIAALHQ
ALALPPALIIGHSMGGAIALQLAITTPQIVAGLGLFGSAARLRVAPALLDGLAGDEATRQATITTLVTWLFSPAADPTLF
AEARAEYAALDPAILLADFQACDGFDVRNRLGDIHCPALVITGSDDRLTPPKLGAELASGLGVPHHLLANVGHMPMLEAP
DQLSELLREWLTTLA
>Mature_254_residues
PLVTTTAGDLFVMRRGTNGVPLIFVHGAGGSGRHWGRLFALLPPTVQFIAVDLPGHGRSPLAGPITIERYAAQIAALHQA
LALPPALIIGHSMGGAIALQLAITTPQIVAGLGLFGSAARLRVAPALLDGLAGDEATRQATITTLVTWLFSPAADPTLFA
EARAEYAALDPAILLADFQACDGFDVRNRLGDIHCPALVITGSDDRLTPPKLGAELASGLGVPHHLLANVGHMPMLEAPD
QLSELLREWLTTLA

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Escherichia coli, GI1787244, Length=218, Percent_Identity=27.9816513761468, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.1

Molecular weight: Translated: 26649; Mature: 26518

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVTTTAGDLFVMRRGTNGVPLIFVHGAGGSGRHWGRLFALLPPTVQFIAVDLPGHGRS
CCEEEECCCCEEEEECCCCCCCEEEEECCCCCCCHHHHEEEECCCCEEEEEEECCCCCCC
PLAGPITIERYAAQIAALHQALALPPALIIGHSMGGAIALQLAITTPQIVAGLGLFGSAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCHHHHHHHHHCCCCH
RLRVAPALLDGLAGDEATRQATITTLVTWLFSPAADPTLFAEARAEYAALDPAILLADFQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEEEEHH
ACDGFDVRNRLGDIHCPALVITGSDDRLTPPKLGAELASGLGVPHHLLANVGHMPMLEAP
CCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
DQLSELLREWLTTLA
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLVTTTAGDLFVMRRGTNGVPLIFVHGAGGSGRHWGRLFALLPPTVQFIAVDLPGHGRS
CEEEECCCCEEEEECCCCCCCEEEEECCCCCCCHHHHEEEECCCCEEEEEEECCCCCCC
PLAGPITIERYAAQIAALHQALALPPALIIGHSMGGAIALQLAITTPQIVAGLGLFGSAA
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCHHHHHHHHHCCCCH
RLRVAPALLDGLAGDEATRQATITTLVTWLFSPAADPTLFAEARAEYAALDPAILLADFQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEEEEHH
ACDGFDVRNRLGDIHCPALVITGSDDRLTPPKLGAELASGLGVPHHLLANVGHMPMLEAP
CCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCC
DQLSELLREWLTTLA
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carboxylic ester; H2O

Specific reaction: A carboxylic ester + H2O = an alcohol + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA