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The map label for this gene is pyk [H]

Identifier: 222526607

GI number: 222526607

Start: 4207233

End: 4208657

Strand: Direct

Name: pyk [H]

Synonym: Chy400_3375

Alternate gene names: 222526607

Gene position: 4207233-4208657 (Clockwise)

Preceding gene: 222526606

Following gene: 222526608

Centisome position: 79.85

GC content: 58.74

Gene sequence:

>1425_bases
ATGCGTCGCACAAAGATTGTTGCCACTCTAGGGCCGGCGACCAGCACCCCTGAACGGATCGCCGGACTGATTCGGGCCGG
TATGAACGTCGCTCGGCTCAACTTCTCGCATGGTACCCATGCCGAGCACGCAGCACGGATTGCAATGGTGCGACGGGCAG
CGGCGGAGGCTGGACGGCATGTAGCGATTCTGCAGGACTTGCAAGGCCCGAAGATTCGTACCGGGCCACTTGAAAATGGG
CAACCGGTTGAACTGGTTGCCGGACAGCGGTTTGTGATTACGACTGAGCCGATCGTTGGCGATGCGCATCGAGTCAGTAC
CACCTATCGTGCGTTGCCACTCGATGTTCGTCCGCGGGATCGGATTCTTCTCTCAGATGGCCTGATCGAGCTGGTTGTGA
CCGGCCATACCGACACCGAAGTGGAGACGGAAGTCGTTCACGGTGGTCGGCTCCGCGAACATCAAGGGATTAACCTGCCG
GGAGTGCGGGTCAGTGCTCCGGCTGCAACCGAGAAAGACCTGGCCGATCTGGCGTTTGGTCTCGAACAGGGAGTAGATTA
TGTCGCGCTCTCGTTTGTTCGGCGCGCCAGTGATGTGCGTGAGGTGAAAGAGTTTATCCGGCGGGCAGGTAAACAAACAC
CGGTCATCGCCAAGATCGAGCGTCCGGAGGCGCTTGACGTGTTGCCGGAGATTCTGGCTGAAGCTGACGGGATCATGGTG
GCGCGTGGTGATCTGGGCGTCGAGATGCCACCGGAGCGGGTGCCGATTGTGCAGAAACAGATTATTGCCGCCGCCAATCA
GGCGCTGCTTCCGGTCATTACGGCGACCCAGATGCTCGAGTCGATGATCCACAACCCGCGACCGACACGGGCTGAGGCCA
GTGATGTGGCGAATGCCATCATTGACGGCACTGATGCCGTTATGCTCAGTGGCGAAACCGCTGCTGGGGCCTATCCGATT
GAAGCGGTGCAGATGATGGCATTAATTGCTGATGCGGTTGAAGCATCGTATACCGGTGGCCAGCACTCGACAACACCGCG
GTGGTCGATTGCGCCGGCGCAATCGACGCCGCGTGCCATTGCCGCAGCAGCATGCACAATCGCCAACTCATTGCCGGTAC
GTTTCATTGTCGTTCTCACCCAAAGCGGTGCGAGTGCCCGTCTGGTATCGCACTACCGGCCTGAAGTGCCAATTCTGGCA
TTCTGTCCCAGTGAAGAGACCGCCCGCCGTACCAGTCTCTACTGGGGTGTCACCCCCATTGTGATCGAGGCGCGTGATCG
CCTCGACGAACTTGAACAACAGATTGTGCGCATGATGCGCGAAACCGGAATGGTTCGAAAAGGCGATTTGATCGTACTGA
GCGGTGGCCATCCGGTTTACCGCTACGGGCCGACCAATTTCCTAAAGGTGATCACGATAGAGTAG

Upstream 100 bases:

>100_bases
GATTGCAAAGGGTATCTACAAACCCGATAACTGCTCAACTGCACCTGTGAAGCAGATGAAAGAAGCCGGTACAGGCATAT
AGCGTCAAATGCGGAGGTAT

Downstream 100 bases:

>100_bases
GGTGCGTTGCAGAAACCTACAAGGCCGGGCAATGATGATCAGTGTCCACAATGACCATCGAGAACAAAAAGCTCGGCCAC
GAAGGGAACTGCACGCCCCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRRTKIVATLGPATSTPERIAGLIRAGMNVARLNFSHGTHAEHAARIAMVRRAAAEAGRHVAILQDLQGPKIRTGPLENG
QPVELVAGQRFVITTEPIVGDAHRVSTTYRALPLDVRPRDRILLSDGLIELVVTGHTDTEVETEVVHGGRLREHQGINLP
GVRVSAPAATEKDLADLAFGLEQGVDYVALSFVRRASDVREVKEFIRRAGKQTPVIAKIERPEALDVLPEILAEADGIMV
ARGDLGVEMPPERVPIVQKQIIAAANQALLPVITATQMLESMIHNPRPTRAEASDVANAIIDGTDAVMLSGETAAGAYPI
EAVQMMALIADAVEASYTGGQHSTTPRWSIAPAQSTPRAIAAAACTIANSLPVRFIVVLTQSGASARLVSHYRPEVPILA
FCPSEETARRTSLYWGVTPIVIEARDRLDELEQQIVRMMRETGMVRKGDLIVLSGGHPVYRYGPTNFLKVITIE

Sequences:

>Translated_474_residues
MRRTKIVATLGPATSTPERIAGLIRAGMNVARLNFSHGTHAEHAARIAMVRRAAAEAGRHVAILQDLQGPKIRTGPLENG
QPVELVAGQRFVITTEPIVGDAHRVSTTYRALPLDVRPRDRILLSDGLIELVVTGHTDTEVETEVVHGGRLREHQGINLP
GVRVSAPAATEKDLADLAFGLEQGVDYVALSFVRRASDVREVKEFIRRAGKQTPVIAKIERPEALDVLPEILAEADGIMV
ARGDLGVEMPPERVPIVQKQIIAAANQALLPVITATQMLESMIHNPRPTRAEASDVANAIIDGTDAVMLSGETAAGAYPI
EAVQMMALIADAVEASYTGGQHSTTPRWSIAPAQSTPRAIAAAACTIANSLPVRFIVVLTQSGASARLVSHYRPEVPILA
FCPSEETARRTSLYWGVTPIVIEARDRLDELEQQIVRMMRETGMVRKGDLIVLSGGHPVYRYGPTNFLKVITIE
>Mature_474_residues
MRRTKIVATLGPATSTPERIAGLIRAGMNVARLNFSHGTHAEHAARIAMVRRAAAEAGRHVAILQDLQGPKIRTGPLENG
QPVELVAGQRFVITTEPIVGDAHRVSTTYRALPLDVRPRDRILLSDGLIELVVTGHTDTEVETEVVHGGRLREHQGINLP
GVRVSAPAATEKDLADLAFGLEQGVDYVALSFVRRASDVREVKEFIRRAGKQTPVIAKIERPEALDVLPEILAEADGIMV
ARGDLGVEMPPERVPIVQKQIIAAANQALLPVITATQMLESMIHNPRPTRAEASDVANAIIDGTDAVMLSGETAAGAYPI
EAVQMMALIADAVEASYTGGQHSTTPRWSIAPAQSTPRAIAAAACTIANSLPVRFIVVLTQSGASARLVSHYRPEVPILA
FCPSEETARRTSLYWGVTPIVIEARDRLDELEQQIVRMMRETGMVRKGDLIVLSGGHPVYRYGPTNFLKVITIE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=43.4077079107505, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=44.1955193482688, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=44.1955193482688, Blast_Score=339, Evalue=4e-93,
Organism=Homo sapiens, GI33286422, Length=504, Percent_Identity=41.8650793650794, Blast_Score=338, Evalue=9e-93,
Organism=Homo sapiens, GI33286420, Length=504, Percent_Identity=41.8650793650794, Blast_Score=338, Evalue=9e-93,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=43.5540069686411, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=43.5540069686411, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=41.2765957446808, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=41.2765957446808, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=41.588785046729, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1787965, Length=458, Percent_Identity=45.1965065502183, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788160, Length=483, Percent_Identity=43.2712215320911, Blast_Score=336, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=41.3223140495868, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=41.5132924335378, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=41.5132924335378, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=41.5132924335378, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=41.5132924335378, Blast_Score=317, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=39.4628099173554, Blast_Score=315, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6324923, Length=494, Percent_Identity=38.4615384615385, Blast_Score=301, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=40.973630831643, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=40.973630831643, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=44.3099273607748, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24581235, Length=495, Percent_Identity=33.5353535353535, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=42.0289855072464, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51231; Mature: 51231

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIVATLGPATSTPERIAGLIRAGMNVARLNFSHGTHAEHAARIAMVRRAAAEAGRH
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCE
VAILQDLQGPKIRTGPLENGQPVELVAGQRFVITTEPIVGDAHRVSTTYRALPLDVRPRD
EEEEECCCCCCEEECCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHEEEEEECCCCCCC
RILLSDGLIELVVTGHTDTEVETEVVHGGRLREHQGINLPGVRVSAPAATEKDLADLAFG
CEEEECCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
LEQGVDYVALSFVRRASDVREVKEFIRRAGKQTPVIAKIERPEALDVLPEILAEADGIMV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEE
ARGDLGVEMPPERVPIVQKQIIAAANQALLPVITATQMLESMIHNPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IDGTDAVMLSGETAAGAYPIEAVQMMALIADAVEASYTGGQHSTTPRWSIAPAQSTPRAI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHH
AAAACTIANSLPVRFIVVLTQSGASARLVSHYRPEVPILAFCPSEETARRTSLYWGVTPI
HHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCCEEEECCCCHHHHHHEEEECCCEE
VIEARDRLDELEQQIVRMMRETGMVRKGDLIVLSGGHPVYRYGPTNFLKVITIE
EEECHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCCEEEEECCCCEEEEEEEC
>Mature Secondary Structure
MRRTKIVATLGPATSTPERIAGLIRAGMNVARLNFSHGTHAEHAARIAMVRRAAAEAGRH
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCE
VAILQDLQGPKIRTGPLENGQPVELVAGQRFVITTEPIVGDAHRVSTTYRALPLDVRPRD
EEEEECCCCCCEEECCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHEEEEEECCCCCCC
RILLSDGLIELVVTGHTDTEVETEVVHGGRLREHQGINLPGVRVSAPAATEKDLADLAFG
CEEEECCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHH
LEQGVDYVALSFVRRASDVREVKEFIRRAGKQTPVIAKIERPEALDVLPEILAEADGIMV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEE
ARGDLGVEMPPERVPIVQKQIIAAANQALLPVITATQMLESMIHNPRPTRAEASDVANAI
EECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IDGTDAVMLSGETAAGAYPIEAVQMMALIADAVEASYTGGQHSTTPRWSIAPAQSTPRAI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHH
AAAACTIANSLPVRFIVVLTQSGASARLVSHYRPEVPILAFCPSEETARRTSLYWGVTPI
HHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCCEEEECCCCHHHHHHEEEECCCEE
VIEARDRLDELEQQIVRMMRETGMVRKGDLIVLSGGHPVYRYGPTNFLKVITIE
EEECHHHHHHHHHHHHHHHHHCCCEEECCEEEEECCCEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA