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The map label for this gene is clpB [H]

Identifier: 222526605

GI number: 222526605

Start: 4203558

End: 4206143

Strand: Reverse

Name: clpB [H]

Synonym: Chy400_3373

Alternate gene names: 222526605

Gene position: 4206143-4203558 (Counterclockwise)

Preceding gene: 222526611

Following gene: 222526599

Centisome position: 79.83

GC content: 58.31

Gene sequence:

>2586_bases
ATGTCCTTCAATACAAATCGTTTTACGCAAAAATCATACGAGGCCATTGCCGCAGCACAGGCAGCAGCCGAGCGGCTCGG
CAATGCGGAAGTTCAACCGGAACACCTGCTCTACGCGCTACTCGACCAGAGTGACGGTGTTGTCCCCCAGGTTCTGGCGA
AGCTGAACCTGCCGGTTGGCGCTTTGAAGCAGCAGATTAATAATGAGATTAGCCGCTTCCCGCGGATGAGTGGCGGTGGC
GCTCAGGTACAGCTCGGTTCGCGGCTCCGCACCGTGTTGATCAAAGCCCACGATGAGCTGGCTCAATTCGGTGATGAATA
TGTCAGCACCGAGCATCTTTTGCTGGCGATCCTCGATCATGCCGGCGGCGGTGCCGAGCGAGTTCTCAAACAGGCCGGTC
TGACCCGCGATAAGCTGTTGATGGTGCTGCGTGAAGTACGAGGTGCTCAGCGCGTTACCAGCCCGACGCCCGAAGGCACC
TATGCGGCGCTGGAACAGTACGGACGTGATCTGACCGAGCTGGCAGCGCGGAATAAGCTTGACCCCGTGATCGGGCGTGA
TGAAGAGATTCGGCGTGTAATCCAGATTCTCTCACGGCGCACGAAGAACAACCCGGTGCTGATCGGTGAGCCGGGTGTTG
GTAAGACGGCCATCGTAGAGGGACTGGCTCAGCGCATTGTACGCGGTGATGTGCCAGAAGCGCTCAAGAATAAACGGGTG
ATTGCACTTGATCTAGGCGCCCTGATTGCCGGCGCTAAATATCGGGGTGAGTTTGAAGAACGACTGAAAGCAGTTCTCAA
AGAGATTCAGGAGCGCGACGATATTATTCTGTTCGTCGACGAGCTGCATACCGTGGTCGGTGCCGGTGCAGCCGAGGGGG
CAATGGATGCCGGGAATATGCTGAAGCCAATGCTGGCCCGCGGTGAGCTGCACATGGTTGGCGCAACCACGCTCGATGAG
TATCGGAAACACATCGAGAAAGATGCCGCGCTTGAACGACGCTTCCAACCGGTGATGGTTGATCCACCGTCGGTTGAAGA
CACCATCTCGATCTTGCGTGGTCTGAAAGAGCGCTACGAGACCCATCACGGTGTGCGCATTACTGACGGTGCGCTGATCG
CGGCGGCAATGCTCTCGGATCGCTACATCTCGGATCGGTTCCTGCCCGACAAAGCTATTGACCTGATCGATGAAGCAGCG
GCCCGTCTGCGCATGGAGATCACATCCGATCCGCAAGAGCTTGACGACCTCAAGCGGCGCATAATGCAATTGGAAATTGA
GCGGGAAGCGCTGCGCAAAGAGAAAGATCAGGCCAGCAAAGAGCGGCTGGAGAAGCTAGAGCAGGAGCTTGCCAATCTGC
GCGAGCAGCGCAGTGCGCTTGAAGCGCAAATCCAGCGCGAACGACAGGAACTTGAGCGTATCCAGCAGCTCAAAGAGAAG
ATCGAACAGACGCGCGCTGCCATCGAGCAGGCCCAACGGCAGTACGACTACAACAAGGCGGCTGAGCTGCAATACGGTAC
TCTGGTCAGCCTGGAGCGCGAATTACAGGCTGCCGAAGCTCAACTTGGTAGCCAGAGCCGCCTGTTGCGCCAGGAAGTGA
CCGAAACCGACATTGCCGAGATCATCTCGAAGTGGACGGGTATTCCGGTCACGAAGCTTCTCGAAGGTGAGCTAGAGAAG
CTGGTGCATATGGAAGAGCGCCTGCATCAGCGGGTAGTCGGTCAGGATGAAGCGGTGATTGCCGTCTCGAACGCAGTACG
GCGTGCGCGCGCCGGCTTGCAAGACCCGAACCGTCCGTTGGGTAGCTTCCTCTTCCTTGGCCCAACCGGTGTGGGTAAAA
CCGAGCTGGCTCGGGCGCTGGCCGAGTTCCTCTTCGACGATGAGCAGGCGATGATCCGGATCGATATGTCCGAGTACATG
GAGAAACACTCCGTTGCTCGACTGATCGGTGCGCCTCCCGGCTACGTTGGCTACGACGAGGGTGGTCAATTGACCGAGGC
GGTGCGGCGCAAACCCTACAGCGTCGTTCTGTTCGATGAAATCGAGAAGGCGCATCCCGATGTCTTCAACGTCTTGCTGC
AAGTGCTCGATGATGGCCGCCTTACCGACGGCCAGGGCCGGATCGTCAACTTCAAGAACACGGTCATCATCATGACCAGT
AACATTGCCAGCACGACGATTCAGGAATTGACGCGCGCCGGCGCCGGCCAAAGCGAAATCCGGGCGGCGGTTATGGAAGA
ATTGCGGGGTGTGCTCCGCCCAGAGTTCTTGAACCGGATCGATGAAGTGATCGTCTTTTCGCCGCTCAGCCGCGAACACA
TTGACCGGATCGTTGAGATTCAGCTCAACCGGTTGCGCAAACTGCTGGCCGACCGTAAGCTCAATCTGGTGCTGACCGAC
GCAGCCCGTGCCCAACTGGCTGCCGAAGGCTATGATCCGGTCTACGGCGCTCGCCCGCTCAAGCGGGTCATCCAGCAACG
CATCCAGAACCCGCTGGCGCTGCAATTGCTCCAGGGGGCCTTCCCGGAGGGCAGCACGATTGTGGTTGATGTCGAGAACG
GGACGTTTGTCTTCCGCCGCAGCTGA

Upstream 100 bases:

>100_bases
AATTCTGAGATTTCCAATAGAACGTTAACATTGCACTATCGCACGACAGATACCGCAATGCTACAGTAACATCAGCACAA
ACTGTTAAGGAGGTGGACGT

Downstream 100 bases:

>100_bases
AAAGTACGTTGCTGAATCTGCATCCAGGACAGACAACCAAGGTTGTCTGTCCTGGATATTATTATGTCAACTTGTTGTCC
TGCTCTATCGCCCCTGACGA

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 861; Mature: 860

Protein sequence:

>861_residues
MSFNTNRFTQKSYEAIAAAQAAAERLGNAEVQPEHLLYALLDQSDGVVPQVLAKLNLPVGALKQQINNEISRFPRMSGGG
AQVQLGSRLRTVLIKAHDELAQFGDEYVSTEHLLLAILDHAGGGAERVLKQAGLTRDKLLMVLREVRGAQRVTSPTPEGT
YAALEQYGRDLTELAARNKLDPVIGRDEEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPEALKNKRV
IALDLGALIAGAKYRGEFEERLKAVLKEIQERDDIILFVDELHTVVGAGAAEGAMDAGNMLKPMLARGELHMVGATTLDE
YRKHIEKDAALERRFQPVMVDPPSVEDTISILRGLKERYETHHGVRITDGALIAAAMLSDRYISDRFLPDKAIDLIDEAA
ARLRMEITSDPQELDDLKRRIMQLEIEREALRKEKDQASKERLEKLEQELANLREQRSALEAQIQRERQELERIQQLKEK
IEQTRAAIEQAQRQYDYNKAAELQYGTLVSLERELQAAEAQLGSQSRLLRQEVTETDIAEIISKWTGIPVTKLLEGELEK
LVHMEERLHQRVVGQDEAVIAVSNAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYM
EKHSVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRIVNFKNTVIIMTS
NIASTTIQELTRAGAGQSEIRAAVMEELRGVLRPEFLNRIDEVIVFSPLSREHIDRIVEIQLNRLRKLLADRKLNLVLTD
AARAQLAAEGYDPVYGARPLKRVIQQRIQNPLALQLLQGAFPEGSTIVVDVENGTFVFRRS

Sequences:

>Translated_861_residues
MSFNTNRFTQKSYEAIAAAQAAAERLGNAEVQPEHLLYALLDQSDGVVPQVLAKLNLPVGALKQQINNEISRFPRMSGGG
AQVQLGSRLRTVLIKAHDELAQFGDEYVSTEHLLLAILDHAGGGAERVLKQAGLTRDKLLMVLREVRGAQRVTSPTPEGT
YAALEQYGRDLTELAARNKLDPVIGRDEEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPEALKNKRV
IALDLGALIAGAKYRGEFEERLKAVLKEIQERDDIILFVDELHTVVGAGAAEGAMDAGNMLKPMLARGELHMVGATTLDE
YRKHIEKDAALERRFQPVMVDPPSVEDTISILRGLKERYETHHGVRITDGALIAAAMLSDRYISDRFLPDKAIDLIDEAA
ARLRMEITSDPQELDDLKRRIMQLEIEREALRKEKDQASKERLEKLEQELANLREQRSALEAQIQRERQELERIQQLKEK
IEQTRAAIEQAQRQYDYNKAAELQYGTLVSLERELQAAEAQLGSQSRLLRQEVTETDIAEIISKWTGIPVTKLLEGELEK
LVHMEERLHQRVVGQDEAVIAVSNAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYM
EKHSVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRIVNFKNTVIIMTS
NIASTTIQELTRAGAGQSEIRAAVMEELRGVLRPEFLNRIDEVIVFSPLSREHIDRIVEIQLNRLRKLLADRKLNLVLTD
AARAQLAAEGYDPVYGARPLKRVIQQRIQNPLALQLLQGAFPEGSTIVVDVENGTFVFRRS
>Mature_860_residues
SFNTNRFTQKSYEAIAAAQAAAERLGNAEVQPEHLLYALLDQSDGVVPQVLAKLNLPVGALKQQINNEISRFPRMSGGGA
QVQLGSRLRTVLIKAHDELAQFGDEYVSTEHLLLAILDHAGGGAERVLKQAGLTRDKLLMVLREVRGAQRVTSPTPEGTY
AALEQYGRDLTELAARNKLDPVIGRDEEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPEALKNKRVI
ALDLGALIAGAKYRGEFEERLKAVLKEIQERDDIILFVDELHTVVGAGAAEGAMDAGNMLKPMLARGELHMVGATTLDEY
RKHIEKDAALERRFQPVMVDPPSVEDTISILRGLKERYETHHGVRITDGALIAAAMLSDRYISDRFLPDKAIDLIDEAAA
RLRMEITSDPQELDDLKRRIMQLEIEREALRKEKDQASKERLEKLEQELANLREQRSALEAQIQRERQELERIQQLKEKI
EQTRAAIEQAQRQYDYNKAAELQYGTLVSLERELQAAEAQLGSQSRLLRQEVTETDIAEIISKWTGIPVTKLLEGELEKL
VHMEERLHQRVVGQDEAVIAVSNAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYME
KHSVARLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRIVNFKNTVIIMTSN
IASTTIQELTRAGAGQSEIRAAVMEELRGVLRPEFLNRIDEVIVFSPLSREHIDRIVEIQLNRLRKLLADRKLNLVLTDA
ARAQLAAEGYDPVYGARPLKRVIQQRIQNPLALQLLQGAFPEGSTIVVDVENGTFVFRRS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=318, Percent_Identity=38.9937106918239, Blast_Score=234, Evalue=3e-61,
Organism=Escherichia coli, GI1788943, Length=859, Percent_Identity=59.3713620488941, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=386, Percent_Identity=41.4507772020725, Blast_Score=283, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=708, Percent_Identity=54.8022598870056, Blast_Score=766, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=868, Percent_Identity=42.3963133640553, Blast_Score=660, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96289; Mature: 96158

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNTNRFTQKSYEAIAAAQAAAERLGNAEVQPEHLLYALLDQSDGVVPQVLAKLNLPVG
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHH
ALKQQINNEISRFPRMSGGGAQVQLGSRLRTVLIKAHDELAQFGDEYVSTEHLLLAILDH
HHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGGAERVLKQAGLTRDKLLMVLREVRGAQRVTSPTPEGTYAALEQYGRDLTELAARNKL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHCCCC
DPVIGRDEEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPEALKNKRV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCE
IALDLGALIAGAKYRGEFEERLKAVLKEIQERDDIILFVDELHTVVGAGAAEGAMDAGNM
EEEEHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHHH
LKPMLARGELHMVGATTLDEYRKHIEKDAALERRFQPVMVDPPSVEDTISILRGLKERYE
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHH
THHGVRITDGALIAAAMLSDRYISDRFLPDKAIDLIDEAAARLRMEITSDPQELDDLKRR
HCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IMQLEIEREALRKEKDQASKERLEKLEQELANLREQRSALEAQIQRERQELERIQQLKEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQTRAAIEQAQRQYDYNKAAELQYGTLVSLERELQAAEAQLGSQSRLLRQEVTETDIAE
HHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IISKWTGIPVTKLLEGELEKLVHMEERLHQRVVGQDEAVIAVSNAVRRARAGLQDPNRPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCC
GSFLFLGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYMEKHSVARLIGAPPGYVGYDE
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGQLTEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRIVNFKNTVIIMTS
CCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEEC
NIASTTIQELTRAGAGQSEIRAAVMEELRGVLRPEFLNRIDEVIVFSPLSREHIDRIVEI
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHH
QLNRLRKLLADRKLNLVLTDAARAQLAAEGYDPVYGARPLKRVIQQRIQNPLALQLLQGA
HHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC
FPEGSTIVVDVENGTFVFRRS
CCCCCEEEEEECCCEEEEECC
>Mature Secondary Structure 
SFNTNRFTQKSYEAIAAAQAAAERLGNAEVQPEHLLYALLDQSDGVVPQVLAKLNLPVG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCHH
ALKQQINNEISRFPRMSGGGAQVQLGSRLRTVLIKAHDELAQFGDEYVSTEHLLLAILDH
HHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGGAERVLKQAGLTRDKLLMVLREVRGAQRVTSPTPEGTYAALEQYGRDLTELAARNKL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHCCCC
DPVIGRDEEIRRVIQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPEALKNKRV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCE
IALDLGALIAGAKYRGEFEERLKAVLKEIQERDDIILFVDELHTVVGAGAAEGAMDAGNM
EEEEHHHHHHCCHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHHH
LKPMLARGELHMVGATTLDEYRKHIEKDAALERRFQPVMVDPPSVEDTISILRGLKERYE
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHH
THHGVRITDGALIAAAMLSDRYISDRFLPDKAIDLIDEAAARLRMEITSDPQELDDLKRR
HCCCEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IMQLEIEREALRKEKDQASKERLEKLEQELANLREQRSALEAQIQRERQELERIQQLKEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEQTRAAIEQAQRQYDYNKAAELQYGTLVSLERELQAAEAQLGSQSRLLRQEVTETDIAE
HHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IISKWTGIPVTKLLEGELEKLVHMEERLHQRVVGQDEAVIAVSNAVRRARAGLQDPNRPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCC
GSFLFLGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYMEKHSVARLIGAPPGYVGYDE
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGQLTEAVRRKPYSVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRIVNFKNTVIIMTS
CCHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEEC
NIASTTIQELTRAGAGQSEIRAAVMEELRGVLRPEFLNRIDEVIVFSPLSREHIDRIVEI
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHH
QLNRLRKLLADRKLNLVLTDAARAQLAAEGYDPVYGARPLKRVIQQRIQNPLALQLLQGA
HHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCC
FPEGSTIVVDVENGTFVFRRS
CCCCCEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]