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The map label for this gene is gatB [H]

Identifier: 222526448

GI number: 222526448

Start: 4013246

End: 4014700

Strand: Direct

Name: gatB [H]

Synonym: Chy400_3215

Alternate gene names: 222526448

Gene position: 4013246-4014700 (Clockwise)

Preceding gene: 222526445

Following gene: 222526449

Centisome position: 76.17

GC content: 56.7

Gene sequence:

>1455_bases
ATGCCAGAGTATATCGCCACTATTGGTCTTGAGATTCATGCTCATGTGTTAACTGCCTCGAAGATGTTCGATGGATGCAG
TACCGATTATGCCGGTGCACCGCCGAACACACGTGTATCGGTGGTGAGTCTCGGTCTCCCTGGTGCACTACCGGTACTGA
ATGCCCGTGCGGTAGAGCTTGCAGCGCTCTGTGGGCTGGCGCTCGGCTGTCGGGTGAACGAGCGCTCAATCTTTGCCCGC
AAGTCGTACCCTTACCCCGATCTGCCGAAGGGCTTTCAAATAACCCAATACGATGAACCACTCTGTAGCGATGGTGCTAT
CGAGATACGGACTGCTGCCGGTGAGGTGCGTCGCATCGGTATCGAGCGGCTGCATATCGAAGAGGATACCGGTCGCCTGA
TTCACAGTCCTGACGGTGCCTCACTCATTGACTACAACCGCAGTGGAATGCCACTGATGGAGATTGTTACCCGACCTGAT
ATTCGTACTCCTGAAGAGGCGCGCCTGACGTTCGAGAAGATTCGGCAAATTCTGGTCTGGATTGGTGCCAACAGTGGTAA
TCTCGAAGAAGGTGCATTGCGCTGTGATGCCAACGTGAGTGTCCGCCCGGCAGGCTCTGAACGCTTTGGGGCGAAGGTTG
AGATCAAGAATATCAATTCGTTCCGGTTTGTGGAACGGGCATTGACGTATGAGATCGAGCGCCAGATCCGCATTTTAGAA
GCCGGTGGTACTGTCGAGCAAGAGACGCGCGGCTGGCGAGAGGATCTTGGCCGTACCGAAGGGCAGCGCTCGAAAGAGTA
CGCGCATGACTACCGGTATTTTCCCGAGCCTGACATTCCGCCACTGGTGCTTTCGCCAGAATGGATCGCTGCTCGCCAGG
CTGAATTACCTGAGCTGCCCGATGCCCGCCGGGCACGGCTGATGGCAACCCACGGCCTATCGTGGCAAGATGCCGATTTG
CTAACGCAGGAACGGGCAATTGCCGATTATTACGAGGCGGCAGTCGCAGCAGTTAACCGTCCTGATGGCGCAAAAGAGGT
TGCGAACTGGGTGTTGAACGAAGGGTTCCGTTTGCTGAAGGATCGGGGCGAGCCGGCAACGGTGCTGATCGAACGGATGC
CGGTACAGCGATTGGCAACGCTCATTGATCTGGTACAGCAGGGCACGATTACCCGCGTCGTGGCCAGGCAGCTCTTCGAG
GAGGTGTTTACGACCGGCGCCGATCCGGCAGCACTGGTGGCCGAGCGTGGTTTGACGCAGATTAGCGATGATGGCGCCTT
GCTGGAAGCAGTACGTGCAGCGCTGGCCGATGAAAAAGCGGCAAAAGCGATTGCTGAATATCGCAAGGGTAAGACAACGG
CCATCCAGTTTCTGGTGGGTATGGTCATGCGGGCAACAAAGGGGCAGGCGAATGCCCAGCGGGTACGTGAATTGTTAGAG
GCGGAACTGCAATGA

Upstream 100 bases:

>100_bases
TTCCGCACTCCAATCATTTACCCGTATATACCACGTAGTCGCGCCATAAGCCGAAAAACCGGGTACAATAGAGGCGATGT
ACCATTACACGAGGAAGCCT

Downstream 100 bases:

>100_bases
CGCTGGCCGGTGACGAGTCTACTACAGTCAATCCACTCACGCTGATGGAGTCGGCGCGGGAAAAATACACCCTGGCGCGA
GCGCGTCGCGAGGTACCGGA

Product: glutamyl-tRNA(Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPEYIATIGLEIHAHVLTASKMFDGCSTDYAGAPPNTRVSVVSLGLPGALPVLNARAVELAALCGLALGCRVNERSIFAR
KSYPYPDLPKGFQITQYDEPLCSDGAIEIRTAAGEVRRIGIERLHIEEDTGRLIHSPDGASLIDYNRSGMPLMEIVTRPD
IRTPEEARLTFEKIRQILVWIGANSGNLEEGALRCDANVSVRPAGSERFGAKVEIKNINSFRFVERALTYEIERQIRILE
AGGTVEQETRGWREDLGRTEGQRSKEYAHDYRYFPEPDIPPLVLSPEWIAARQAELPELPDARRARLMATHGLSWQDADL
LTQERAIADYYEAAVAAVNRPDGAKEVANWVLNEGFRLLKDRGEPATVLIERMPVQRLATLIDLVQQGTITRVVARQLFE
EVFTTGADPAALVAERGLTQISDDGALLEAVRAALADEKAAKAIAEYRKGKTTAIQFLVGMVMRATKGQANAQRVRELLE
AELQ

Sequences:

>Translated_484_residues
MPEYIATIGLEIHAHVLTASKMFDGCSTDYAGAPPNTRVSVVSLGLPGALPVLNARAVELAALCGLALGCRVNERSIFAR
KSYPYPDLPKGFQITQYDEPLCSDGAIEIRTAAGEVRRIGIERLHIEEDTGRLIHSPDGASLIDYNRSGMPLMEIVTRPD
IRTPEEARLTFEKIRQILVWIGANSGNLEEGALRCDANVSVRPAGSERFGAKVEIKNINSFRFVERALTYEIERQIRILE
AGGTVEQETRGWREDLGRTEGQRSKEYAHDYRYFPEPDIPPLVLSPEWIAARQAELPELPDARRARLMATHGLSWQDADL
LTQERAIADYYEAAVAAVNRPDGAKEVANWVLNEGFRLLKDRGEPATVLIERMPVQRLATLIDLVQQGTITRVVARQLFE
EVFTTGADPAALVAERGLTQISDDGALLEAVRAALADEKAAKAIAEYRKGKTTAIQFLVGMVMRATKGQANAQRVRELLE
AELQ
>Mature_483_residues
PEYIATIGLEIHAHVLTASKMFDGCSTDYAGAPPNTRVSVVSLGLPGALPVLNARAVELAALCGLALGCRVNERSIFARK
SYPYPDLPKGFQITQYDEPLCSDGAIEIRTAAGEVRRIGIERLHIEEDTGRLIHSPDGASLIDYNRSGMPLMEIVTRPDI
RTPEEARLTFEKIRQILVWIGANSGNLEEGALRCDANVSVRPAGSERFGAKVEIKNINSFRFVERALTYEIERQIRILEA
GGTVEQETRGWREDLGRTEGQRSKEYAHDYRYFPEPDIPPLVLSPEWIAARQAELPELPDARRARLMATHGLSWQDADLL
TQERAIADYYEAAVAAVNRPDGAKEVANWVLNEGFRLLKDRGEPATVLIERMPVQRLATLIDLVQQGTITRVVARQLFEE
VFTTGADPAALVAERGLTQISDDGALLEAVRAALADEKAAKAIAEYRKGKTTAIQFLVGMVMRATKGQANAQRVRELLEA
ELQ

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=504, Percent_Identity=36.1111111111111, Blast_Score=301, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=39.1304347826087, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6319391, Length=525, Percent_Identity=30.4761904761905, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI45550808, Length=504, Percent_Identity=35.3174603174603, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53509; Mature: 53377

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYIATIGLEIHAHVLTASKMFDGCSTDYAGAPPNTRVSVVSLGLPGALPVLNARAVEL
CCCCEEECCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
AALCGLALGCRVNERSIFARKSYPYPDLPKGFQITQYDEPLCSDGAIEIRTAAGEVRRIG
HHHHHHHHCCEECCCCEEEECCCCCCCCCCCCEECCCCCCCCCCCCEEEEECHHHHHHHC
IERLHIEEDTGRLIHSPDGASLIDYNRSGMPLMEIVTRPDIRTPEEARLTFEKIRQILVW
HHHEEEECCCCCEEECCCCCCEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHEEE
IGANSGNLEEGALRCDANVSVRPAGSERFGAKVEIKNINSFRFVERALTYEIERQIRILE
EECCCCCCCCCCEEECCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEE
AGGTVEQETRGWREDLGRTEGQRSKEYAHDYRYFPEPDIPPLVLSPEWIAARQAELPELP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCCCC
DARRARLMATHGLSWQDADLLTQERAIADYYEAAVAAVNRPDGAKEVANWVLNEGFRLLK
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
DRGEPATVLIERMPVQRLATLIDLVQQGTITRVVARQLFEEVFTTGADPAALVAERGLTQ
CCCCCCEEHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
ISDDGALLEAVRAALADEKAAKAIAEYRKGKTTAIQFLVGMVMRATKGQANAQRVRELLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AELQ
HHCC
>Mature Secondary Structure 
PEYIATIGLEIHAHVLTASKMFDGCSTDYAGAPPNTRVSVVSLGLPGALPVLNARAVEL
CCCEEECCCEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
AALCGLALGCRVNERSIFARKSYPYPDLPKGFQITQYDEPLCSDGAIEIRTAAGEVRRIG
HHHHHHHHCCEECCCCEEEECCCCCCCCCCCCEECCCCCCCCCCCCEEEEECHHHHHHHC
IERLHIEEDTGRLIHSPDGASLIDYNRSGMPLMEIVTRPDIRTPEEARLTFEKIRQILVW
HHHEEEECCCCCEEECCCCCCEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHEEE
IGANSGNLEEGALRCDANVSVRPAGSERFGAKVEIKNINSFRFVERALTYEIERQIRILE
EECCCCCCCCCCEEECCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHEEEEE
AGGTVEQETRGWREDLGRTEGQRSKEYAHDYRYFPEPDIPPLVLSPEWIAARQAELPELP
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCCCC
DARRARLMATHGLSWQDADLLTQERAIADYYEAAVAAVNRPDGAKEVANWVLNEGFRLLK
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
DRGEPATVLIERMPVQRLATLIDLVQQGTITRVVARQLFEEVFTTGADPAALVAERGLTQ
CCCCCCEEHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
ISDDGALLEAVRAALADEKAAKAIAEYRKGKTTAIQFLVGMVMRATKGQANAQRVRELLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AELQ
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA