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The map label for this gene is yhxA [H]

Identifier: 222526071

GI number: 222526071

Start: 3492822

End: 3494267

Strand: Direct

Name: yhxA [H]

Synonym: Chy400_2828

Alternate gene names: 222526071

Gene position: 3492822-3494267 (Clockwise)

Preceding gene: 222526070

Following gene: 222526075

Centisome position: 66.29

GC content: 57.47

Gene sequence:

>1446_bases
ATGAGTACCATTACGAACAATCGACGTCAGGCCCTCAGTGATAATGTCTGGTTACATTTCTTCCAAATGGGCCACTTCTC
CAGTACCGACGCGCGGCCCACAATACTGGTCCGAGGTGAAGGGTCGCGCGTATGGGATCAAGACGGTAACGAGTACATCG
ATGGTCTGTCCGGTCTGTTTACAGTCAATGTCGGTTATGGTCGCCGTGAAATCATCGAGGCGATCAGTGCGCAACTGAGC
GAGATTGCTTACGTCAGTCCTTTCAGCTTCCCCAGTCTGCCTTTAATTGACATATCGGCGCGCCTGGCTTCAATCAGTCC
CACCGGCCCCCGCTCGCGAGTATTTCTGACCACCGGGGGTTCAGATGCAGTGGAGACTGCATTGAAGCTGGCCAAAGCGT
ACCAGCGGCGGCGCGGGTTTGCCGACCGCACCAAGATTATTGCCCGTCGCGTGAGCTATCACGGCACCTCAATGGGTGCG
CTATCGGTCAACGGTGTCACCTCCATCCGTAACGGTTTTGGCCCGCTGGTACCGGGGGCACGCCATGCGCCCCTCCCCTA
TCGTTTTCGCTGCGACTATTGCGCCACTCACAGTGGCTGCCGTGGTGTCTGTGTCGATGAAGTCGAGCGTCTGATCGAGT
TTGAAGGCCCTGAAACAATTGCCGCCATTATTATGGAACCGGTGCAAAATAGTGGCGGTGCAATTGTCTCGCCTCCCGGC
TATCTACAACGCATTCGCCAGATCTGCGATCACTACGGGATCGTCATGATTGTTGATGAAGTGATCTGCGGTTTTGGCCG
GGTTGGTGATTGGTTTGGTTCAACAGCGATGGGGGTCAGGCCCGACATCATCACCTGCGCCAAAGGCATCACCAGCGGCT
ACGCTCCGCTCGGTGCGGCCATTGTCTGTGACACGCTCGCCGATGTCTTCGTTTCCGATAATGAAGCAGATAAGTTTATG
CACGGAATCACCTTTGGTGGGCACGCTGCCAGTTGTGCGGCAGCACTCGCCAACCTGGACATTATCGAACGGGAACATCT
GCTGGAGCGGTCGCGCGAAATGGGAGCCTACCTGATGCAAGAGCTAACCGCTGCCGTTGGCAATCACCCCAACGTGGGTG
AGGTGCGGGGGATGGGCATGTTTATGGCTGTCGAGCTGGTACGTGACCGGGTCACCCGCGAGTCACTGGCCGAGGAGCGG
CTGATGATCTGGCTGAGTGATCAATTGAAGCAGCGTGGTCTGATTTGCCGGGCCGATGATCGACTCGAACCGGTGATCCA
ACTGGCGCCGCCGTTGATCCTCACCCGCGAAGAAGCTGATCGCTGTGTCAGCATCGTCGCCGAGGCCGTCCATGCCCTCG
GTCGTCGACTGGGAAGCGTCCCAACCGTCATCTCGCTCAACGTCACAACACCGGTAGAACCACCGACAGCGGTGGCGGCT
GATTAA

Upstream 100 bases:

>100_bases
TGTGCCTACGATGTGGTCTCTCTCTCTTACCTGCTGCGGTGTCGCAGTCGCGCATTCGGCGCGTTAGAGGCCACGGTTGC
GGACGTAGTCAGGAATCTAT

Downstream 100 bases:

>100_bases
AAAGAGCAAGAAAGGTGCGATGCATCGTCACATGCATCGCACCTTCTTTCATCCGGCAACCGTTGTCTGCCGGATGCCAA
CCTCTCGCGTTAATAGTCCG

Product: class III aminotransferase

Products: L-Glutamate; L-Glutamate 5-semialdehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSTITNNRRQALSDNVWLHFFQMGHFSSTDARPTILVRGEGSRVWDQDGNEYIDGLSGLFTVNVGYGRREIIEAISAQLS
EIAYVSPFSFPSLPLIDISARLASISPTGPRSRVFLTTGGSDAVETALKLAKAYQRRRGFADRTKIIARRVSYHGTSMGA
LSVNGVTSIRNGFGPLVPGARHAPLPYRFRCDYCATHSGCRGVCVDEVERLIEFEGPETIAAIIMEPVQNSGGAIVSPPG
YLQRIRQICDHYGIVMIVDEVICGFGRVGDWFGSTAMGVRPDIITCAKGITSGYAPLGAAIVCDTLADVFVSDNEADKFM
HGITFGGHAASCAAALANLDIIEREHLLERSREMGAYLMQELTAAVGNHPNVGEVRGMGMFMAVELVRDRVTRESLAEER
LMIWLSDQLKQRGLICRADDRLEPVIQLAPPLILTREEADRCVSIVAEAVHALGRRLGSVPTVISLNVTTPVEPPTAVAA
D

Sequences:

>Translated_481_residues
MSTITNNRRQALSDNVWLHFFQMGHFSSTDARPTILVRGEGSRVWDQDGNEYIDGLSGLFTVNVGYGRREIIEAISAQLS
EIAYVSPFSFPSLPLIDISARLASISPTGPRSRVFLTTGGSDAVETALKLAKAYQRRRGFADRTKIIARRVSYHGTSMGA
LSVNGVTSIRNGFGPLVPGARHAPLPYRFRCDYCATHSGCRGVCVDEVERLIEFEGPETIAAIIMEPVQNSGGAIVSPPG
YLQRIRQICDHYGIVMIVDEVICGFGRVGDWFGSTAMGVRPDIITCAKGITSGYAPLGAAIVCDTLADVFVSDNEADKFM
HGITFGGHAASCAAALANLDIIEREHLLERSREMGAYLMQELTAAVGNHPNVGEVRGMGMFMAVELVRDRVTRESLAEER
LMIWLSDQLKQRGLICRADDRLEPVIQLAPPLILTREEADRCVSIVAEAVHALGRRLGSVPTVISLNVTTPVEPPTAVAA
D
>Mature_480_residues
STITNNRRQALSDNVWLHFFQMGHFSSTDARPTILVRGEGSRVWDQDGNEYIDGLSGLFTVNVGYGRREIIEAISAQLSE
IAYVSPFSFPSLPLIDISARLASISPTGPRSRVFLTTGGSDAVETALKLAKAYQRRRGFADRTKIIARRVSYHGTSMGAL
SVNGVTSIRNGFGPLVPGARHAPLPYRFRCDYCATHSGCRGVCVDEVERLIEFEGPETIAAIIMEPVQNSGGAIVSPPGY
LQRIRQICDHYGIVMIVDEVICGFGRVGDWFGSTAMGVRPDIITCAKGITSGYAPLGAAIVCDTLADVFVSDNEADKFMH
GITFGGHAASCAAALANLDIIEREHLLERSREMGAYLMQELTAAVGNHPNVGEVRGMGMFMAVELVRDRVTRESLAEERL
MIWLSDQLKQRGLICRADDRLEPVIQLAPPLILTREEADRCVSIVAEAVHALGRRLGSVPTVISLNVTTPVEPPTAVAAD

Specific function: Essential for glycerol catabolism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=431, Percent_Identity=29.4663573085847, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI226442705, Length=431, Percent_Identity=29.6983758700696, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI13994255, Length=438, Percent_Identity=28.7671232876712, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI226442709, Length=390, Percent_Identity=30.2564102564103, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI24119277, Length=421, Percent_Identity=29.4536817102138, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI4557809, Length=440, Percent_Identity=26.8181818181818, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI284507298, Length=333, Percent_Identity=28.2282282282282, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI188536080, Length=385, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI38679950, Length=385, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI38679946, Length=385, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI145693181, Length=469, Percent_Identity=31.3432835820896, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789016, Length=424, Percent_Identity=31.3679245283019, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789759, Length=352, Percent_Identity=31.5340909090909, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1787560, Length=417, Percent_Identity=29.4964028776978, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788044, Length=412, Percent_Identity=27.4271844660194, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1786991, Length=421, Percent_Identity=31.353919239905, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1786349, Length=278, Percent_Identity=29.136690647482, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564660, Length=441, Percent_Identity=27.6643990929705, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25144271, Length=427, Percent_Identity=26.6978922716628, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71992977, Length=446, Percent_Identity=26.6816143497758, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25144274, Length=306, Percent_Identity=28.1045751633987, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17541228, Length=445, Percent_Identity=24.4943820224719, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323470, Length=428, Percent_Identity=28.0373831775701, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6324432, Length=374, Percent_Identity=28.3422459893048, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324386, Length=430, Percent_Identity=26.2790697674419, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21356575, Length=420, Percent_Identity=30.2380952380952, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28574759, Length=468, Percent_Identity=27.991452991453, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI161085790, Length=467, Percent_Identity=28.051391862955, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21357415, Length=424, Percent_Identity=26.8867924528302, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI281366494, Length=390, Percent_Identity=22.8205128205128, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667139, Length=390, Percent_Identity=22.8205128205128, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667143, Length=390, Percent_Identity=22.8205128205128, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.13 [C]

Molecular weight: Translated: 52247; Mature: 52116

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTITNNRRQALSDNVWLHFFQMGHFSSTDARPTILVRGEGSRVWDQDGNEYIDGLSGLF
CCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEE
TVNVGYGRREIIEAISAQLSEIAYVSPFSFPSLPLIDISARLASISPTGPRSRVFLTTGG
EEECCCCHHHHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHCCCCCCCCCEEEEEECC
SDAVETALKLAKAYQRRRGFADRTKIIARRVSYHGTSMGALSVNGVTSIRNGFGPLVPGA
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCC
RHAPLPYRFRCDYCATHSGCRGVCVDEVERLIEFEGPETIAAIIMEPVQNSGGAIVSPPG
CCCCCCEEEECCHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCH
YLQRIRQICDHYGIVMIVDEVICGFGRVGDWFGSTAMGVRPDIITCAKGITSGYAPLGAA
HHHHHHHHHHHCCHHEEHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHH
IVCDTLADVFVSDNEADKFMHGITFGGHAASCAAALANLDIIEREHLLERSREMGAYLMQ
HHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELTAAVGNHPNVGEVRGMGMFMAVELVRDRVTRESLAEERLMIWLSDQLKQRGLICRADD
HHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCCEEECCC
RLEPVIQLAPPLILTREEADRCVSIVAEAVHALGRRLGSVPTVISLNVTTPVEPPTAVAA
CHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
D
C
>Mature Secondary Structure 
STITNNRRQALSDNVWLHFFQMGHFSSTDARPTILVRGEGSRVWDQDGNEYIDGLSGLF
CCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEE
TVNVGYGRREIIEAISAQLSEIAYVSPFSFPSLPLIDISARLASISPTGPRSRVFLTTGG
EEECCCCHHHHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHCCCCCCCCCEEEEEECC
SDAVETALKLAKAYQRRRGFADRTKIIARRVSYHGTSMGALSVNGVTSIRNGFGPLVPGA
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCC
RHAPLPYRFRCDYCATHSGCRGVCVDEVERLIEFEGPETIAAIIMEPVQNSGGAIVSPPG
CCCCCCEEEECCHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCH
YLQRIRQICDHYGIVMIVDEVICGFGRVGDWFGSTAMGVRPDIITCAKGITSGYAPLGAA
HHHHHHHHHHHCCHHEEHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHH
IVCDTLADVFVSDNEADKFMHGITFGGHAASCAAALANLDIIEREHLLERSREMGAYLMQ
HHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELTAAVGNHPNVGEVRGMGMFMAVELVRDRVTRESLAEERLMIWLSDQLKQRGLICRADD
HHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCCEEECCC
RLEPVIQLAPPLILTREEADRCVSIVAEAVHALGRRLGSVPTVISLNVTTPVEPPTAVAA
CHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; Ornithine [C]

Specific reaction: 2-Oxoglutarate + Ornithine --> L-Glutamate + L-Glutamate 5-semialdehyde [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8969498; 8436953 [H]