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The map label for this gene is lepB [H]

Identifier: 222524676

GI number: 222524676

Start: 1786227

End: 1786937

Strand: Direct

Name: lepB [H]

Synonym: Chy400_1403

Alternate gene names: 222524676

Gene position: 1786227-1786937 (Clockwise)

Preceding gene: 222524669

Following gene: 222524677

Centisome position: 33.9

GC content: 50.21

Gene sequence:

>711_bases
ATGACGAATAGTCCTTCATCATCAGAACCGCTCACGAGTGATCCGGAAACTGTTCAAGTTCGTCAACCTCGTGCCCCTCT
GCGGTACGTGGTTCGTGAACTGCTCGAAACAGCTATCTTTATTCTGCTGGTTTTTCTCATCGTGCGCGGTGTCGTGCAAA
ATTTCAAGATTGAAGGCTCAAGTATGGAGCCAACGCTCCACACCGGTCAATATATTTTAGTCAACAAACTCATCTATTTT
CATTTCGATTTAAACGCACCACTACGCCTGCTTCCCGGCCAATCTGATCTTCCACCCCGTATTGTATATCCTTTTCGCCC
TCCCCAACGCGGTGACATTGTTGTCTTTGAGTATCCACGTGATGTACGTAAAGATTATATCAAACGAGTTATTGGTTTGC
CGGGTGACATTATCGAGATTCGTGAAGGTAAAGTCTTTGTCAATAACGAACCGCTCGATGAGCCATACCTGCGCGGTGCG
TCCACCTATTGTCTGGGTGGGTATCCCTGCGCCCAGGGACCGGTCCTGGTGCCAGCCGGTAGTATCTTCGTGATGGGTGA
CAATCGCGGCAATAGTTCCGACTCGCGAGAGTGGGATGCCTTACCGCTGGATCGGGTTGTCGGTCAGGCATGGCTCATCT
ACTTTCCGTTTAGTGATTGGGGATTGGTACCGCACCATCGCTACGAGACCAGTACTGCGGTAGCACCTTAG

Upstream 100 bases:

>100_bases
TATAATGCCATGGGTGGCGGAGGGAAACCTTCCCTCCGCATACCATCTTTGGCTTTACGTGCTATCTCTTAGTGTAGCAT
AGAGTGTCCACGTTCATGCT

Downstream 100 bases:

>100_bases
TCAACCGCTCAATCGTATGCATATCGCTATCAACGCTCATCTGCTTGCGCATACCCACTCGTTTCGGCGGGCCGGTGTTT
CTCACTATATCGAACAAGTC

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTNSPSSSEPLTSDPETVQVRQPRAPLRYVVRELLETAIFILLVFLIVRGVVQNFKIEGSSMEPTLHTGQYILVNKLIYF
HFDLNAPLRLLPGQSDLPPRIVYPFRPPQRGDIVVFEYPRDVRKDYIKRVIGLPGDIIEIREGKVFVNNEPLDEPYLRGA
STYCLGGYPCAQGPVLVPAGSIFVMGDNRGNSSDSREWDALPLDRVVGQAWLIYFPFSDWGLVPHHRYETSTAVAP

Sequences:

>Translated_236_residues
MTNSPSSSEPLTSDPETVQVRQPRAPLRYVVRELLETAIFILLVFLIVRGVVQNFKIEGSSMEPTLHTGQYILVNKLIYF
HFDLNAPLRLLPGQSDLPPRIVYPFRPPQRGDIVVFEYPRDVRKDYIKRVIGLPGDIIEIREGKVFVNNEPLDEPYLRGA
STYCLGGYPCAQGPVLVPAGSIFVMGDNRGNSSDSREWDALPLDRVVGQAWLIYFPFSDWGLVPHHRYETSTAVAP
>Mature_235_residues
TNSPSSSEPLTSDPETVQVRQPRAPLRYVVRELLETAIFILLVFLIVRGVVQNFKIEGSSMEPTLHTGQYILVNKLIYFH
FDLNAPLRLLPGQSDLPPRIVYPFRPPQRGDIVVFEYPRDVRKDYIKRVIGLPGDIIEIREGKVFVNNEPLDEPYLRGAS
TYCLGGYPCAQGPVLVPAGSIFVMGDNRGNSSDSREWDALPLDRVVGQAWLIYFPFSDWGLVPHHRYETSTAVAP

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=99, Percent_Identity=40.4040404040404, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 26625; Mature: 26494

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00501 SPASE_I_1 ; PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSPSSSEPLTSDPETVQVRQPRAPLRYVVRELLETAIFILLVFLIVRGVVQNFKIEGS
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCC
SMEPTLHTGQYILVNKLIYFHFDLNAPLRLLPGQSDLPPRIVYPFRPPQRGDIVVFEYPR
CCCCCCCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCEEEECCCCCCCCCEEEEECCH
DVRKDYIKRVIGLPGDIIEIREGKVFVNNEPLDEPYLRGASTYCLGGYPCAQGPVLVPAG
HHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCHHHCCCCEEEECCCCCCCCCEEEECC
SIFVMGDNRGNSSDSREWDALPLDRVVGQAWLIYFPFSDWGLVPHHRYETSTAVAP
EEEEEECCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNSPSSSEPLTSDPETVQVRQPRAPLRYVVRELLETAIFILLVFLIVRGVVQNFKIEGS
CCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCC
SMEPTLHTGQYILVNKLIYFHFDLNAPLRLLPGQSDLPPRIVYPFRPPQRGDIVVFEYPR
CCCCCCCCCCEEEEEEEEEEEEECCCCEEECCCCCCCCCEEEECCCCCCCCCEEEEECCH
DVRKDYIKRVIGLPGDIIEIREGKVFVNNEPLDEPYLRGASTYCLGGYPCAQGPVLVPAG
HHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCHHHCCCCEEEECCCCCCCCCEEEECC
SIFVMGDNRGNSSDSREWDALPLDRVVGQAWLIYFPFSDWGLVPHHRYETSTAVAP
EEEEEECCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1546969 [H]