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The map label for this gene is rnhA [H]

Identifier: 222524669

GI number: 222524669

Start: 1778347

End: 1779141

Strand: Direct

Name: rnhA [H]

Synonym: Chy400_1396

Alternate gene names: 222524669

Gene position: 1778347-1779141 (Clockwise)

Preceding gene: 222524668

Following gene: 222524676

Centisome position: 33.75

GC content: 57.23

Gene sequence:

>795_bases
ATGGCGCAAAAAAAGCAATTCTATCTTGTGGTACGTGGCCGAAATCCCGGTCTCTATCAGCAGTGGTCAGGTGAGGATGG
TGCATCTGCTCAGGTAACCGGCTTTCCCGGCGCACTCTACAAAGGCTTTTCAACCATAGCCGCCGCCCACGAGTGGGTAA
GATCGTTGCCTGCGACCGAACAGACGCTGGCACTGCAATTGCTCGCTGACTACGAGCATGCGCCACACGCCGAAGCTGAA
CCGGATCAGAACACGCAACAGCCATTGTCACGACCGGCAACATCCCCCTCAGAGGACGCTTCTTCTTCCTCCACAAACGG
GATTGTGATGATGTACACCGACGGTAGTGCTCTCGGCAATCCTGGGCCGGGCGGGTACGGCGTTGTGCTGCGCTACAATG
ACCATTACCAGGAACTCACCGGTGGTTTTCGGCTCACGACCAACAACCGCATGGAGTTAATGGCCTGTATTGTCGGGCTG
CGTGCACTCAAGCGACCGGTGTCGGTACGCCTGCACAGCGACTCGCGCTACGTCGTCGATGCTTTTCGCAAAGGCTGGAT
ACACCGCTGGCAGGCACAAAACTGGATGCGCAGCGAGACCGAACCGGTAAAAAATGCCGATCTCTGGGCTGAATTGCGCT
TGCTGTGTGAAGGTCATCAGGTTGAGTTCGTCTGGGTGCCCGGTCATCAGGGCGTACCTGACAATGAACGTTGTCACGAA
CTGGCCATCGCTGCCGCAGCTCGCCCAGACCTGCCGCCGGATGTTGCCTTTGAGATGCAGTGGAGTCGCACATAA

Upstream 100 bases:

>100_bases
AAGTCAAGGGTCGAAGCGCAGAAGTTTGTGGCGAGTTTCAGATACGTGCAAAAAAGATGGTATAGTAACGCCGGTATCGA
GTACAGAACCACAACAATGT

Downstream 100 bases:

>100_bases
GCGCAATGATACCGGCGTGGATGTGCGAGCTGTGGGAGCCTCTGCACCGTACCTATGCGCACGAGCGGCATACCTGCTTC
ACATGCTCAGGTTTCTACAG

Product: Ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAQKKQFYLVVRGRNPGLYQQWSGEDGASAQVTGFPGALYKGFSTIAAAHEWVRSLPATEQTLALQLLADYEHAPHAEAE
PDQNTQQPLSRPATSPSEDASSSSTNGIVMMYTDGSALGNPGPGGYGVVLRYNDHYQELTGGFRLTTNNRMELMACIVGL
RALKRPVSVRLHSDSRYVVDAFRKGWIHRWQAQNWMRSETEPVKNADLWAELRLLCEGHQVEFVWVPGHQGVPDNERCHE
LAIAAAARPDLPPDVAFEMQWSRT

Sequences:

>Translated_264_residues
MAQKKQFYLVVRGRNPGLYQQWSGEDGASAQVTGFPGALYKGFSTIAAAHEWVRSLPATEQTLALQLLADYEHAPHAEAE
PDQNTQQPLSRPATSPSEDASSSSTNGIVMMYTDGSALGNPGPGGYGVVLRYNDHYQELTGGFRLTTNNRMELMACIVGL
RALKRPVSVRLHSDSRYVVDAFRKGWIHRWQAQNWMRSETEPVKNADLWAELRLLCEGHQVEFVWVPGHQGVPDNERCHE
LAIAAAARPDLPPDVAFEMQWSRT
>Mature_263_residues
AQKKQFYLVVRGRNPGLYQQWSGEDGASAQVTGFPGALYKGFSTIAAAHEWVRSLPATEQTLALQLLADYEHAPHAEAEP
DQNTQQPLSRPATSPSEDASSSSTNGIVMMYTDGSALGNPGPGGYGVVLRYNDHYQELTGGFRLTTNNRMELMACIVGLR
ALKRPVSVRLHSDSRYVVDAFRKGWIHRWQAQNWMRSETEPVKNADLWAELRLLCEGHQVEFVWVPGHQGVPDNERCHEL
AIAAAARPDLPPDVAFEMQWSRT

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=266, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=49.3421052631579, Blast_Score=154, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI115532542, Length=262, Percent_Identity=28.2442748091603, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 29403; Mature: 29272

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKKQFYLVVRGRNPGLYQQWSGEDGASAQVTGFPGALYKGFSTIAAAHEWVRSLPATE
CCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCH
QTLALQLLADYEHAPHAEAEPDQNTQQPLSRPATSPSEDASSSSTNGIVMMYTDGSALGN
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
PGPGGYGVVLRYNDHYQELTGGFRLTTNNRMELMACIVGLRALKRPVSVRLHSDSRYVVD
CCCCCEEEEEEECCCHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
AFRKGWIHRWQAQNWMRSETEPVKNADLWAELRLLCEGHQVEFVWVPGHQGVPDNERCHE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHH
LAIAAAARPDLPPDVAFEMQWSRT
HHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
AQKKQFYLVVRGRNPGLYQQWSGEDGASAQVTGFPGALYKGFSTIAAAHEWVRSLPATE
CCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCH
QTLALQLLADYEHAPHAEAEPDQNTQQPLSRPATSPSEDASSSSTNGIVMMYTDGSALGN
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
PGPGGYGVVLRYNDHYQELTGGFRLTTNNRMELMACIVGLRALKRPVSVRLHSDSRYVVD
CCCCCEEEEEEECCCHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHH
AFRKGWIHRWQAQNWMRSETEPVKNADLWAELRLLCEGHQVEFVWVPGHQGVPDNERCHE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHH
LAIAAAARPDLPPDVAFEMQWSRT
HHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA