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The map label for this gene is rlmI [H]

Identifier: 222524646

GI number: 222524646

Start: 1751424

End: 1752626

Strand: Direct

Name: rlmI [H]

Synonym: Chy400_1371

Alternate gene names: 222524646

Gene position: 1751424-1752626 (Clockwise)

Preceding gene: 222524645

Following gene: 222524647

Centisome position: 33.24

GC content: 57.52

Gene sequence:

>1203_bases
ATGACAACTGTTATCATCCTTCCCGGTCGCGAACGACCGATTATACAGCGACATCCATGGATCTTTTCCGGTGCTATCGC
CGAAGTACGTGGTAATCCCGCGCCCGGCGATGTGGTTGATGTATGCGCAAAAGATGGGAGCTGGCTGGCCCGTGGCTTCT
GGAGCAGCACGTCGCAACTGCGAGTACGGCTGGCGACCTGGGATGCAAACCAGCCCCTCGATGAAACCTGGCTCCGCGAG
ACTATTGCCCGGGCTGTAGCCGGGCGCGAATACTGGCTTCAGAACCCGGCGATTGCCTGCCGGCTTGTGTTCAGCGAGTC
GGATGGCCTACCCGGCCTGATTGTTGACCGCTACGGCTGCTATCTGGTGATTCAACTACTCACTCAGGCGATGGCGGTGC
GCGCCGATCAAGTGATTGCAGCGTTAGTCGATCTGCTGGCACCACGCGGTATCTACGAGCGGAGTGATGCTGAGGTACGT
GAGAAGGAAGGACTGCCACCGGCGGTTGGTTTACGCTGGGGCGAAACCCCGCCGCGATTGCCGGTCACGCTCATCACTGC
ACTCGATCAACCGGTCACCAGACGGCGCATGTTCGCCGACCTGCAAACCGGCCAGAAGACCGGCATTTATCTCGATCAGG
CGATCAACCACCTGCGGGTGGGGGCGTATTGTGCGGACGCCGATACCCTCGATTGTTTCTCCTACGCGGGCGGCTTTGCC
CTGGCCGCCGCACTGGCCGGCGCTCGTCAGTTGACACTGGTTGATGCCAGTGCCGAAGCACTGGGGTTGGCGGCTGATAA
TCTGCGGCTCAACAACCTGTCAACACCAACCACCTTTGTCGAGGGTGATGTGTTTCAGGTGTTACGCCGGTATCGCAGCG
ATCAGCGCCAATTTGATGTCGTTATTCTCGACCCACCGAAATTCGTTCATCAACAGGCACATTTACAACGGGCGACACGC
GGCTACAAAGATATTAATTTGCAGGCACTCCACCTGATCCGACGCGGTGGCATCCTGGCTACCTTTAGTTGTTCGGGGTT
GGTTTCAGCCGATCTGTTTCAAAAAATCATCTTCGGGGCTGCGGTTGATGCCGGTCGCGATGTGCAAATTCTGGAGCGAC
TGACGCAGGCACCCGATCATCCGGTATTGCTGAGTTTTCCTGAAGCGGAATATCTCAAAGGTTTAATCTGCCGGGTGTGG
TAA

Upstream 100 bases:

>100_bases
GATCCAACAGGGTCTCTCGGTGTAAATTTGTTCACAGATCGCATAGAGACGGTATCGTCTGCTTGACCGCACTGCAAGCA
CGTGATAGATTTAATCTATT

Downstream 100 bases:

>100_bases
ACTATAGACACTCGTATGTAGTTGCAGAGCAGGTATGAACTTCACGATCTTTGAAGATGGTCACATTGCCAGCCCAACCG
GCTTTCGCGCCACCGGTGTT

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTTVIILPGRERPIIQRHPWIFSGAIAEVRGNPAPGDVVDVCAKDGSWLARGFWSSTSQLRVRLATWDANQPLDETWLRE
TIARAVAGREYWLQNPAIACRLVFSESDGLPGLIVDRYGCYLVIQLLTQAMAVRADQVIAALVDLLAPRGIYERSDAEVR
EKEGLPPAVGLRWGETPPRLPVTLITALDQPVTRRRMFADLQTGQKTGIYLDQAINHLRVGAYCADADTLDCFSYAGGFA
LAAALAGARQLTLVDASAEALGLAADNLRLNNLSTPTTFVEGDVFQVLRRYRSDQRQFDVVILDPPKFVHQQAHLQRATR
GYKDINLQALHLIRRGGILATFSCSGLVSADLFQKIIFGAAVDAGRDVQILERLTQAPDHPVLLSFPEAEYLKGLICRVW

Sequences:

>Translated_400_residues
MTTVIILPGRERPIIQRHPWIFSGAIAEVRGNPAPGDVVDVCAKDGSWLARGFWSSTSQLRVRLATWDANQPLDETWLRE
TIARAVAGREYWLQNPAIACRLVFSESDGLPGLIVDRYGCYLVIQLLTQAMAVRADQVIAALVDLLAPRGIYERSDAEVR
EKEGLPPAVGLRWGETPPRLPVTLITALDQPVTRRRMFADLQTGQKTGIYLDQAINHLRVGAYCADADTLDCFSYAGGFA
LAAALAGARQLTLVDASAEALGLAADNLRLNNLSTPTTFVEGDVFQVLRRYRSDQRQFDVVILDPPKFVHQQAHLQRATR
GYKDINLQALHLIRRGGILATFSCSGLVSADLFQKIIFGAAVDAGRDVQILERLTQAPDHPVLLSFPEAEYLKGLICRVW
>Mature_399_residues
TTVIILPGRERPIIQRHPWIFSGAIAEVRGNPAPGDVVDVCAKDGSWLARGFWSSTSQLRVRLATWDANQPLDETWLRET
IARAVAGREYWLQNPAIACRLVFSESDGLPGLIVDRYGCYLVIQLLTQAMAVRADQVIAALVDLLAPRGIYERSDAEVRE
KEGLPPAVGLRWGETPPRLPVTLITALDQPVTRRRMFADLQTGQKTGIYLDQAINHLRVGAYCADADTLDCFSYAGGFAL
AAALAGARQLTLVDASAEALGLAADNLRLNNLSTPTTFVEGDVFQVLRRYRSDQRQFDVVILDPPKFVHQQAHLQRATRG
YKDINLQALHLIRRGGILATFSCSGLVSADLFQKIIFGAAVDAGRDVQILERLTQAPDHPVLLSFPEAEYLKGLICRVW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=400, Percent_Identity=44.25, Blast_Score=351, Evalue=4e-98,
Organism=Escherichia coli, GI1787181, Length=233, Percent_Identity=27.4678111587983, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF01472 PUA [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44151; Mature: 44020

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVIILPGRERPIIQRHPWIFSGAIAEVRGNPAPGDVVDVCAKDGSWLARGFWSSTSQL
CEEEEEECCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHCCCCCEEHHHHCCCCCEE
RVRLATWDANQPLDETWLRETIARAVAGREYWLQNPAIACRLVFSESDGLPGLIVDRYGC
EEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHH
YLVIQLLTQAMAVRADQVIAALVDLLAPRGIYERSDAEVREKEGLPPAVGLRWGETPPRL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
PVTLITALDQPVTRRRMFADLQTGQKTGIYLDQAINHLRVGAYCADADTLDCFSYAGGFA
CCHHEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHEECEEECCCCCHHHHHHCCHHH
LAAALAGARQLTLVDASAEALGLAADNLRLNNLSTPTTFVEGDVFQVLRRYRSDQRQFDV
HHHHHHCCCEEEEEECCCHHHEEEECCEEECCCCCCCEEECCHHHHHHHHHHCCCCEEEE
VILDPPKFVHQQAHLQRATRGYKDINLQALHLIRRGGILATFSCSGLVSADLFQKIIFGA
EEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
AVDAGRDVQILERLTQAPDHPVLLSFPEAEYLKGLICRVW
HCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
TTVIILPGRERPIIQRHPWIFSGAIAEVRGNPAPGDVVDVCAKDGSWLARGFWSSTSQL
EEEEEECCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHCCCCCEEHHHHCCCCCEE
RVRLATWDANQPLDETWLRETIARAVAGREYWLQNPAIACRLVFSESDGLPGLIVDRYGC
EEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHH
YLVIQLLTQAMAVRADQVIAALVDLLAPRGIYERSDAEVREKEGLPPAVGLRWGETPPRL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
PVTLITALDQPVTRRRMFADLQTGQKTGIYLDQAINHLRVGAYCADADTLDCFSYAGGFA
CCHHEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHEECEEECCCCCHHHHHHCCHHH
LAAALAGARQLTLVDASAEALGLAADNLRLNNLSTPTTFVEGDVFQVLRRYRSDQRQFDV
HHHHHHCCCEEEEEECCCHHHEEEECCEEECCCCCCCEEECCHHHHHHHHHHCCCCEEEE
VILDPPKFVHQQAHLQRATRGYKDINLQALHLIRRGGILATFSCSGLVSADLFQKIIFGA
EEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHH
AVDAGRDVQILERLTQAPDHPVLLSFPEAEYLKGLICRVW
HCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA