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The map label for this gene is nuoG2 [H]

Identifier: 222524575

GI number: 222524575

Start: 1665392

End: 1666099

Strand: Direct

Name: nuoG2 [H]

Synonym: Chy400_1299

Alternate gene names: 222524575

Gene position: 1665392-1666099 (Clockwise)

Preceding gene: 222524574

Following gene: 222524576

Centisome position: 31.61

GC content: 57.49

Gene sequence:

>708_bases
ATGGCGGCTAAAACACTCACAATTGATGGCCATCTCGTCTCGGCCCGCCCCGGTGCAACGCTATTGGAAGCTATCCGCGA
CGCTGGTATTCCCATTCCTACCCTTTGTCATCTCGACGGATTATCGGATGTCGGCGCGTGTCGGCTCTGTCTGGTTGAGA
TCGAAGGTCAACGTCGTCTGCAACCGGCCTGTATGACGATGGTTAGCGAGGGGATGGTCGTCCACACCGCGACACCCAGG
CTCCAGAATTATCGGCGTCAAATTATCGAGCTGTTGTTCGCCGAACGCAATCACGTGTGCTCTGTATGCGTAGCCAGTGG
CAACTGCGAACTGCAACGATTAGCGGCGATGGTGGGGATGGATCACGTTCGCTACGAATACCTCTCTCCTGCCTGTCCCG
TTGACATCTCGCACCCACGGTTCGGCATCGACCACAATCGTTGTGTGCTCTGTACGCGCTGCGTGCGCGCCTGTGACGAA
ATTGAAGGTGTTCATACCTGGGATGTGGCCGGACGCGGGGTTGATGCAAGAGTCATCACCGATATGAACCAGCCCTGGGG
AACTTCCACCACCTGCACCAGTTGCGGGAAATGCCAGTTGGCCTGCCCAACCGGTGCCATTTTCCCGCGTGGTGTGACCG
TTGGCGAACGTCCACACGACAGTGAACGCATCGCTCTGATTGTCGAAGCGCGGAAGCAACGCTGGTAG

Upstream 100 bases:

>100_bases
AGCCTCTGTGGCCTGGGCCAATCAGCGCCAAACCCGGTACTTAGCACCCTACACTACTTCCGACACGAATACGAACAGCT
CATCGGAGCGGAGGTTGATC

Downstream 100 bases:

>100_bases
GTGCTGCGATAGAACGAACCGCAGGTGAGAGGGAGAAGTCTATGAGCCGATTACGGATTGCTACCATCTGGTTGGGTGGC
TGTTCGGGCTGCCATATGTC

Product: bidirectional hydrogenase complex protein HoxU

Products: NA

Alternate protein names: NADH dehydrogenase I subunit G 2; NDH-1 subunit G 2 [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MAAKTLTIDGHLVSARPGATLLEAIRDAGIPIPTLCHLDGLSDVGACRLCLVEIEGQRRLQPACMTMVSEGMVVHTATPR
LQNYRRQIIELLFAERNHVCSVCVASGNCELQRLAAMVGMDHVRYEYLSPACPVDISHPRFGIDHNRCVLCTRCVRACDE
IEGVHTWDVAGRGVDARVITDMNQPWGTSTTCTSCGKCQLACPTGAIFPRGVTVGERPHDSERIALIVEARKQRW

Sequences:

>Translated_235_residues
MAAKTLTIDGHLVSARPGATLLEAIRDAGIPIPTLCHLDGLSDVGACRLCLVEIEGQRRLQPACMTMVSEGMVVHTATPR
LQNYRRQIIELLFAERNHVCSVCVASGNCELQRLAAMVGMDHVRYEYLSPACPVDISHPRFGIDHNRCVLCTRCVRACDE
IEGVHTWDVAGRGVDARVITDMNQPWGTSTTCTSCGKCQLACPTGAIFPRGVTVGERPHDSERIALIVEARKQRW
>Mature_234_residues
AAKTLTIDGHLVSARPGATLLEAIRDAGIPIPTLCHLDGLSDVGACRLCLVEIEGQRRLQPACMTMVSEGMVVHTATPRL
QNYRRQIIELLFAERNHVCSVCVASGNCELQRLAAMVGMDHVRYEYLSPACPVDISHPRFGIDHNRCVLCTRCVRACDEI
EGVHTWDVAGRGVDARVITDMNQPWGTSTTCTSCGKCQLACPTGAIFPRGVTVGERPHDSERIALIVEARKQRW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1034

COG function: function code C; NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI33519475, Length=220, Percent_Identity=31.3636363636364, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI145693161, Length=255, Percent_Identity=27.843137254902, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17565758, Length=219, Percent_Identity=29.2237442922374, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI193209088, Length=219, Percent_Identity=29.2237442922374, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32566231, Length=219, Percent_Identity=29.2237442922374, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24640559, Length=224, Percent_Identity=29.0178571428571, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24640557, Length=224, Percent_Identity=29.0178571428571, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009010
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR000283
- InterPro:   IPR019574 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10588 NADH-G_4Fe-4S_3 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25776; Mature: 25645

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTLTIDGHLVSARPGATLLEAIRDAGIPIPTLCHLDGLSDVGACRLCLVEIEGQRRL
CCCEEEEECCEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCC
QPACMTMVSEGMVVHTATPRLQNYRRQIIELLFAERNHVCSVCVASGNCELQRLAAMVGM
CHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
DHVRYEYLSPACPVDISHPRFGIDHNRCVLCTRCVRACDEIEGVHTWDVAGRGVDARVIT
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
DMNQPWGTSTTCTSCGKCQLACPTGAIFPRGVTVGERPHDSERIALIVEARKQRW
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
AAKTLTIDGHLVSARPGATLLEAIRDAGIPIPTLCHLDGLSDVGACRLCLVEIEGQRRL
CCEEEEECCEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCC
QPACMTMVSEGMVVHTATPRLQNYRRQIIELLFAERNHVCSVCVASGNCELQRLAAMVGM
CHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
DHVRYEYLSPACPVDISHPRFGIDHNRCVLCTRCVRACDEIEGVHTWDVAGRGVDARVIT
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEE
DMNQPWGTSTTCTSCGKCQLACPTGAIFPRGVTVGERPHDSERIALIVEARKQRW
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]