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The map label for this gene is cheB4 [H]

Identifier: 222524200

GI number: 222524200

Start: 1164485

End: 1165528

Strand: Reverse

Name: cheB4 [H]

Synonym: Chy400_0921

Alternate gene names: 222524200

Gene position: 1165528-1164485 (Counterclockwise)

Preceding gene: 222524201

Following gene: 222524199

Centisome position: 22.12

GC content: 54.5

Gene sequence:

>1044_bases
ATGAGTATCAAAGAACCAATTCGGGTTTTGTTAGTTGAAGATTCACCCAGTCAGTTACAACTTCTGCGCTCGTATCTTGA
GGCAACACCTGATCTGGTGGTAGTAGGCACCGCTATCAATGGTCGGGATGCTGTCCAATCAACGCTGCGACTGCATCCAC
ACGTGGTAGCAATGGACATCCATTTGCCGGTGTTCGATGGGTATGAAGCAACCCGGCAAATTATGCAACAACGCCCAACA
CCGATAGTGCTCTTCAGTTCGAGCACCGGCGATGCGGAACAACGCGCTATGGCTGCACTGGCAGCCGGCGCCCTGGCGGT
CGTTCGTAAACCGTCTGGCCCTGTCGATCCGGCCAGTGCTGCTGATCGTGACCATTTGATCCGCACGTTACGTTTAATGG
CTGGGGTCAAGGTGGTAACCCGCCATCCACCGCGTATGACGCCGAAGCAGATAGATCAGGCCAACAAGGAATATGCCATC
CTGGCTATCGCGGCCTCAACCGGTGGTCCGGCAGCGCTGCAAACGATCCTCCGTGATTTGGGAAGCAGTTTCCCGCTGCC
GATTGTGATTGCGCAACACGTGACATCAGGATTTATGCGAGCTTTTGCTGATTGGCTTGCCAGTACCATTCCATTGTCGG
TTCATGTTGTCGAACAAGAGACGCCGATCCTTCCGGGCCATGTGTATCTGCCGTGCGATGATCACCACCTTGAGATTGTC
CGGCAGCATGTGGTTACCATAAAGAGGTCTACGCCGCATGATCGCTATTGCCCGAGTGCTGACCATTTGTTTAGCAGCGT
GGCGCATCATTACGGATCACGTGCTATTGGCGTGATTTTGACCGGGATGGGTGACGATGGTGCCCAGGGGTTGCTGGAAC
TGGCGCGAGCCGGTGGGACAACGTTTGGTCAGGATGAGGCTTCGTGTGTTGTTTATGGGATGCCGAAGATGGCTAAAGTC
CGCGGTGCTGTTCATCATGAATTACCACTGGAACAATTGGGGATGGCGGTTTCGGCAGTGCTGGGAAAGGGGGGAAGGGG
ATAG

Upstream 100 bases:

>100_bases
CGGTTTAACCTGTGGCCATCGGAATGTGTGCAGTTTGTGAAACCGGATTATTGAGGTTACACCTGGAAAAGCCTTCTTTC
AGCAGTGCCGGGCAACCTGT

Downstream 100 bases:

>100_bases
GAGATAGGAGATAGGAAATAGGAGAGAGGAGAGAGGAGAGAGGAGAGAGGAGAGAGGAGAGAGGAAATAGGAGGTAGGAG
AGAGGAGAGAGAAGAGAGAA

Product: response regulator receiver modulated CheB methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSIKEPIRVLLVEDSPSQLQLLRSYLEATPDLVVVGTAINGRDAVQSTLRLHPHVVAMDIHLPVFDGYEATRQIMQQRPT
PIVLFSSSTGDAEQRAMAALAAGALAVVRKPSGPVDPASAADRDHLIRTLRLMAGVKVVTRHPPRMTPKQIDQANKEYAI
LAIAASTGGPAALQTILRDLGSSFPLPIVIAQHVTSGFMRAFADWLASTIPLSVHVVEQETPILPGHVYLPCDDHHLEIV
RQHVVTIKRSTPHDRYCPSADHLFSSVAHHYGSRAIGVILTGMGDDGAQGLLELARAGGTTFGQDEASCVVYGMPKMAKV
RGAVHHELPLEQLGMAVSAVLGKGGRG

Sequences:

>Translated_347_residues
MSIKEPIRVLLVEDSPSQLQLLRSYLEATPDLVVVGTAINGRDAVQSTLRLHPHVVAMDIHLPVFDGYEATRQIMQQRPT
PIVLFSSSTGDAEQRAMAALAAGALAVVRKPSGPVDPASAADRDHLIRTLRLMAGVKVVTRHPPRMTPKQIDQANKEYAI
LAIAASTGGPAALQTILRDLGSSFPLPIVIAQHVTSGFMRAFADWLASTIPLSVHVVEQETPILPGHVYLPCDDHHLEIV
RQHVVTIKRSTPHDRYCPSADHLFSSVAHHYGSRAIGVILTGMGDDGAQGLLELARAGGTTFGQDEASCVVYGMPKMAKV
RGAVHHELPLEQLGMAVSAVLGKGGRG
>Mature_346_residues
SIKEPIRVLLVEDSPSQLQLLRSYLEATPDLVVVGTAINGRDAVQSTLRLHPHVVAMDIHLPVFDGYEATRQIMQQRPTP
IVLFSSSTGDAEQRAMAALAAGALAVVRKPSGPVDPASAADRDHLIRTLRLMAGVKVVTRHPPRMTPKQIDQANKEYAIL
AIAASTGGPAALQTILRDLGSSFPLPIVIAQHVTSGFMRAFADWLASTIPLSVHVVEQETPILPGHVYLPCDDHHLEIVR
QHVVTIKRSTPHDRYCPSADHLFSSVAHHYGSRAIGVILTGMGDDGAQGLLELARAGGTTFGQDEASCVVYGMPKMAKVR
GAVHHELPLEQLGMAVSAVLGKGGRG

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=346, Percent_Identity=34.6820809248555, Blast_Score=188, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 37200; Mature: 37069

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKEPIRVLLVEDSPSQLQLLRSYLEATPDLVVVGTAINGRDAVQSTLRLHPHVVAMDI
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEEE
HLPVFDGYEATRQIMQQRPTPIVLFSSSTGDAEQRAMAALAAGALAVVRKPSGPVDPASA
ECCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC
ADRDHLIRTLRLMAGVKVVTRHPPRMTPKQIDQANKEYAILAIAASTGGPAALQTILRDL
CCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHH
GSSFPLPIVIAQHVTSGFMRAFADWLASTIPLSVHVVEQETPILPGHVYLPCDDHHLEIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEECCCCHHHHHH
RQHVVTIKRSTPHDRYCPSADHLFSSVAHHYGSRAIGVILTGMGDDGAQGLLELARAGGT
HHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCC
TFGQDEASCVVYGMPKMAKVRGAVHHELPLEQLGMAVSAVLGKGGRG
CCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIKEPIRVLLVEDSPSQLQLLRSYLEATPDLVVVGTAINGRDAVQSTLRLHPHVVAMDI
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEEE
HLPVFDGYEATRQIMQQRPTPIVLFSSSTGDAEQRAMAALAAGALAVVRKPSGPVDPASA
ECCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCC
ADRDHLIRTLRLMAGVKVVTRHPPRMTPKQIDQANKEYAILAIAASTGGPAALQTILRDL
CCHHHHHHHHHHHHCCHHEECCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHH
GSSFPLPIVIAQHVTSGFMRAFADWLASTIPLSVHVVEQETPILPGHVYLPCDDHHLEIV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEECCCCHHHHHH
RQHVVTIKRSTPHDRYCPSADHLFSSVAHHYGSRAIGVILTGMGDDGAQGLLELARAGGT
HHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCC
TFGQDEASCVVYGMPKMAKVRGAVHHELPLEQLGMAVSAVLGKGGRG
CCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA