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The map label for this gene is narX [C]

Identifier: 222524044

GI number: 222524044

Start: 947554

End: 949191

Strand: Reverse

Name: narX [C]

Synonym: Chy400_0759

Alternate gene names: 222524044

Gene position: 949191-947554 (Counterclockwise)

Preceding gene: 222524048

Following gene: 222524043

Centisome position: 18.01

GC content: 55.68

Gene sequence:

>1638_bases
ATGCCGCCGCTTGCGATCAATCAGCGCCAACTGGAAACGCTCAGTCAACTGCTTATTGCCGGCGGTCACCACGAAGTGGC
TGATCTCTTGCAGGCATCGCTTGATCGCCTGATCGCTTTCTGGCCGGCTCAGGCCGGTGCATTGCTCTACGTTTCGCCAT
ACGGTGAAACGACGAAGGTGATGCACGGCAAACTCGATGCTGAAACGTTGTTGTTGATCGAGCAGGCGCGCGATGGCTTC
ACCCGTCGTGACGATAATGGCGAACCGATTGTCGGCTCGTATTCGATTGACGAGGGGCGTGACCTTATCGAGTTGCCATT
ACAGAGCGGCGGGCAGGGTGTGGGACTGCTGCACCTGGTGGTCAGCCGTAACAATCAGCAGTCGGGGGGGCATCCACCCC
TGGATGAGGAGTTGCTTGTGCTGCTGGTGCGGGCAATTGGTGGTGAAGCCGATAAGCTGGCCATGTTGCGCCGGGCCGAG
CGGGATTTACGCGAGCTGCATCTACTCTACGAGATCGGCCAGTCACTGGCAGTGAATCTCGATCTCGCCAGTCTGCTGAA
CGATATTAAGTTACGCGCCCCCAAAGTTGTTGGGGCCGAGCGCTGCTCAATCTTTATTCTCGATGAAGAGACGAATGAGT
TAGTCCTCGAAATTCCCGGCGAGCAGCGTCAGTATCGGATGCCGGCTGATCGTGGGATTGCCGGTTGGGTGGTGACGCAT
GGCGTTGGTCAGATTGTGAATGATGTCGAACACGATCCGCGCTGGTACGATGCGATTAGCCGTGAAGCCGATTTTGTCAC
CCGCTCGATAGTGTGTGTGCCGATGCGGGTGAAGGATCGGGTGATCGGTGCCATGCAGTTGTTGAATAAGGCGAACGGTC
AGCCGTTCACCGATCAGGATATGCAATTGCTGACAACGCTGGCGGCTCAGGCGGCCATTGCTATCGAGAATGCCCGCCTC
TATCAACGGCTGAAGGAAGAGCGTGACCGGCTGTTGCTGAAAGAGGCCGAGGTACGCCACGCTATTGCGCGCGATTTGCA
CGATGGCCCCACTCAGAGTATCGCGGCAATCGCGATGAATATTGAGTTCATTAAGCGTCTGTTCAAGGCCATGCCTGAAC
GAGTTCCGGCTGAACTCGATACCCTGGCTGAACTGGTGCAAAAAACGACCCACGACATTCGCACGTTACTGTTCGAGTTG
CGTCCTCTCGGCCTCGAAACGCAGGGTCTGCTGGCAACATTGCAACAGTACGTTGAACGTTGGCGCGATCCATCTGGTAG
TGATACGAAGTTGCGCCTGGAAGCACCGGCTCAGGTGCCACGCCTGTCACCCGAAGTTGAAGCAGCCGTCTTCATTATTT
TGCAAGAGGCGGTGAATAATGCCCGCAAGCACGCGCACAGTGATACGATCACTATCTATTTGTATGTTGAAGAGGATCAC
TTTGTAGCCAGTGTGCGTGATCGCGGGCGGGGCTTCAATGTTGCTGCGGTGGAAAGTGGCTATGTGAATCGCGGTAGTCT
CGGCCTGTTGAATATGAAAGAACGGGCACGTCTGATTGGTGCCGATCTGCGTATTCGTAGTGAGATCGGTCAGGGGACAA
CGGTTGAATTGCGTATTCCACTGAGTCAAGCGCGTTGA

Upstream 100 bases:

>100_bases
TTATGATTACAGTACCATTACAATATACGCCCTCATACGTTATAATACGCATTATACATATTACTGGATCGGAAACTCTT
GCCACAATTCGAGGTGCCTT

Downstream 100 bases:

>100_bases
TGCAGGGTTTGGCCGCGACAGACGACGCTCGCCTTGCCTGCATCCCGGTCATCCGCCAAGACCTGCGCAGGTTGAGTTTG
GTTGCCGTCTACCCGACACA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MPPLAINQRQLETLSQLLIAGGHHEVADLLQASLDRLIAFWPAQAGALLYVSPYGETTKVMHGKLDAETLLLIEQARDGF
TRRDDNGEPIVGSYSIDEGRDLIELPLQSGGQGVGLLHLVVSRNNQQSGGHPPLDEELLVLLVRAIGGEADKLAMLRRAE
RDLRELHLLYEIGQSLAVNLDLASLLNDIKLRAPKVVGAERCSIFILDEETNELVLEIPGEQRQYRMPADRGIAGWVVTH
GVGQIVNDVEHDPRWYDAISREADFVTRSIVCVPMRVKDRVIGAMQLLNKANGQPFTDQDMQLLTTLAAQAAIAIENARL
YQRLKEERDRLLLKEAEVRHAIARDLHDGPTQSIAAIAMNIEFIKRLFKAMPERVPAELDTLAELVQKTTHDIRTLLFEL
RPLGLETQGLLATLQQYVERWRDPSGSDTKLRLEAPAQVPRLSPEVEAAVFIILQEAVNNARKHAHSDTITIYLYVEEDH
FVASVRDRGRGFNVAAVESGYVNRGSLGLLNMKERARLIGADLRIRSEIGQGTTVELRIPLSQAR

Sequences:

>Translated_545_residues
MPPLAINQRQLETLSQLLIAGGHHEVADLLQASLDRLIAFWPAQAGALLYVSPYGETTKVMHGKLDAETLLLIEQARDGF
TRRDDNGEPIVGSYSIDEGRDLIELPLQSGGQGVGLLHLVVSRNNQQSGGHPPLDEELLVLLVRAIGGEADKLAMLRRAE
RDLRELHLLYEIGQSLAVNLDLASLLNDIKLRAPKVVGAERCSIFILDEETNELVLEIPGEQRQYRMPADRGIAGWVVTH
GVGQIVNDVEHDPRWYDAISREADFVTRSIVCVPMRVKDRVIGAMQLLNKANGQPFTDQDMQLLTTLAAQAAIAIENARL
YQRLKEERDRLLLKEAEVRHAIARDLHDGPTQSIAAIAMNIEFIKRLFKAMPERVPAELDTLAELVQKTTHDIRTLLFEL
RPLGLETQGLLATLQQYVERWRDPSGSDTKLRLEAPAQVPRLSPEVEAAVFIILQEAVNNARKHAHSDTITIYLYVEEDH
FVASVRDRGRGFNVAAVESGYVNRGSLGLLNMKERARLIGADLRIRSEIGQGTTVELRIPLSQAR
>Mature_544_residues
PPLAINQRQLETLSQLLIAGGHHEVADLLQASLDRLIAFWPAQAGALLYVSPYGETTKVMHGKLDAETLLLIEQARDGFT
RRDDNGEPIVGSYSIDEGRDLIELPLQSGGQGVGLLHLVVSRNNQQSGGHPPLDEELLVLLVRAIGGEADKLAMLRRAER
DLRELHLLYEIGQSLAVNLDLASLLNDIKLRAPKVVGAERCSIFILDEETNELVLEIPGEQRQYRMPADRGIAGWVVTHG
VGQIVNDVEHDPRWYDAISREADFVTRSIVCVPMRVKDRVIGAMQLLNKANGQPFTDQDMQLLTTLAAQAAIAIENARLY
QRLKEERDRLLLKEAEVRHAIARDLHDGPTQSIAAIAMNIEFIKRLFKAMPERVPAELDTLAELVQKTTHDIRTLLFELR
PLGLETQGLLATLQQYVERWRDPSGSDTKLRLEAPAQVPRLSPEVEAAVFIILQEAVNNARKHAHSDTITIYLYVEEDHF
VASVRDRGRGFNVAAVESGYVNRGSLGLLNMKERARLIGADLRIRSEIGQGTTVELRIPLSQAR

Specific function: Acts As A Sensor For Nitrate/Nitrite And Transduces Signal Of Nitrate Availability To The Narl Protein And Of Both Nitrate/Nitrite To The Narp Protein. Narx Probably Activates Narl And Narp By Phosphorylation In The Presence Of Nitrate. Narx Also Plays A

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Homo sapiens, GI4505657, Length=226, Percent_Identity=34.070796460177, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI219802706, Length=226, Percent_Identity=34.070796460177, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI226246591, Length=226, Percent_Identity=34.070796460177, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI5729972, Length=154, Percent_Identity=29.8701298701299, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI194595488, Length=154, Percent_Identity=29.8701298701299, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI116536085, Length=191, Percent_Identity=29.3193717277487, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI116536087, Length=288, Percent_Identity=23.9583333333333, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI61744432, Length=183, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI61744430, Length=183, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI61744435, Length=183, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787474, Length=246, Percent_Identity=27.6422764227642, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193202333, Length=327, Percent_Identity=21.4067278287462, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 60726; Mature: 60594

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLAINQRQLETLSQLLIAGGHHEVADLLQASLDRLIAFWPAQAGALLYVSPYGETTKV
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
MHGKLDAETLLLIEQARDGFTRRDDNGEPIVGSYSIDEGRDLIELPLQSGGQGVGLLHLV
HCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEECCHHCCCCCCEEEEEE
VSRNNQQSGGHPPLDEELLVLLVRAIGGEADKLAMLRRAERDLRELHLLYEIGQSLAVNL
EECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
DLASLLNDIKLRAPKVVGAERCSIFILDEETNELVLEIPGEQRQYRMPADRGIAGWVVTH
EHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
GVGQIVNDVEHDPRWYDAISREADFVTRSIVCVPMRVKDRVIGAMQLLNKANGQPFTDQD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHH
MQLLTTLAAQAAIAIENARLYQRLKEERDRLLLKEAEVRHAIARDLHDGPTQSIAAIAMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IEFIKRLFKAMPERVPAELDTLAELVQKTTHDIRTLLFELRPLGLETQGLLATLQQYVER
HHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
WRDPSGSDTKLRLEAPAQVPRLSPEVEAAVFIILQEAVNNARKHAHSDTITIYLYVEEDH
HCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
FVASVRDRGRGFNVAAVESGYVNRGSLGLLNMKERARLIGADLRIRSEIGQGTTVELRIP
EEEEHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHCCCCCEEEEEEC
LSQAR
CCCCC
>Mature Secondary Structure 
PPLAINQRQLETLSQLLIAGGHHEVADLLQASLDRLIAFWPAQAGALLYVSPYGETTKV
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
MHGKLDAETLLLIEQARDGFTRRDDNGEPIVGSYSIDEGRDLIELPLQSGGQGVGLLHLV
HCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCEEECCHHCCCCCCEEEEEE
VSRNNQQSGGHPPLDEELLVLLVRAIGGEADKLAMLRRAERDLRELHLLYEIGQSLAVNL
EECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
DLASLLNDIKLRAPKVVGAERCSIFILDEETNELVLEIPGEQRQYRMPADRGIAGWVVTH
EHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
GVGQIVNDVEHDPRWYDAISREADFVTRSIVCVPMRVKDRVIGAMQLLNKANGQPFTDQD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCHH
MQLLTTLAAQAAIAIENARLYQRLKEERDRLLLKEAEVRHAIARDLHDGPTQSIAAIAMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IEFIKRLFKAMPERVPAELDTLAELVQKTTHDIRTLLFELRPLGLETQGLLATLQQYVER
HHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
WRDPSGSDTKLRLEAPAQVPRLSPEVEAAVFIILQEAVNNARKHAHSDTITIYLYVEEDH
HCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
FVASVRDRGRGFNVAAVESGYVNRGSLGLLNMKERARLIGADLRIRSEIGQGTTVELRIP
EEEEHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHCCCCCEEEEEEC
LSQAR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661927 [H]