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The map label for this gene is ntcA [H]

Identifier: 222523877

GI number: 222523877

Start: 701452

End: 702165

Strand: Reverse

Name: ntcA [H]

Synonym: Chy400_0586

Alternate gene names: 222523877

Gene position: 702165-701452 (Counterclockwise)

Preceding gene: 222523878

Following gene: 222523873

Centisome position: 13.33

GC content: 52.94

Gene sequence:

>714_bases
GTGGGTACGTTGCAATCGGATCGTCTGGACGACAAGGTGCGTTATCTGCGTGATATTGACATCTTTCGCGATCTTGATCC
AGATGAGATTGAATTGCTGGGGAAACGGGCACCGATGCAGCGTGTTCCAGCCGGAACCGTCTTCTTTTCCCCGGAACAAC
GCGCAGAGGTGCTGTTCATCCTCAAAGAGGGTCGTGTACGCATCTATCGCCTCTCAACCGATGGCAAGATGCTCACAACC
GCCATCCTCAACGAAGGAACAATCTTCGGTGAGATGGCGCTGCTTGGCCAACAACTGCACCAGCACTATGCCGAAGCACT
CACTCCCTGTCTGCTCTGCCTGATGAGTCGCGAAGATGTGAAGCGCCTCTTGCTGAGTGATCCACGGATTGCGACTCGCA
TCGTTGAGATTCTGGGTCAGCGTTTGATCAGTGCTGAACAACGGTTATCCGATTTTGCCTTCAAGAGTTTACCGCAGCGG
ATCGCCACGCTCCTGCTACAGATGGCTCAACCCGCCAAACCGCGCCTCTTCCGTCCCGGTAGTTCCCAGCGGGAAGTTGC
CCTGACCCATGAAGCGTTAGCCGAAATGCTCGGTACATATCGCGAAACCACAACCAAAATTCTGAACGATCTTCGTACAC
AGGGATTGATTGAGTTGCGGCGAGGTCGAATTGTCCTGTTAGATGAAATTGGCTTGCAAAAACTGAGCGAATAA

Upstream 100 bases:

>100_bases
TGAAACAGTCATCGCGTTAGGTAATCCAGAACTATCAACACTGCTGGCCTGGCTTGATCACATTGCACGGGTGTCAATAT
AAATCGCGAAAGGAATGAGC

Downstream 100 bases:

>100_bases
GGGTTTGTTGGGTAATGCTAAATCCGGTTTACTGTAAGTCATCGCTCATGATCGCACTGTCACACTAGAACTTATTTCAT
AAAACGCTGTACTGTCGCAC

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MGTLQSDRLDDKVRYLRDIDIFRDLDPDEIELLGKRAPMQRVPAGTVFFSPEQRAEVLFILKEGRVRIYRLSTDGKMLTT
AILNEGTIFGEMALLGQQLHQHYAEALTPCLLCLMSREDVKRLLLSDPRIATRIVEILGQRLISAEQRLSDFAFKSLPQR
IATLLLQMAQPAKPRLFRPGSSQREVALTHEALAEMLGTYRETTTKILNDLRTQGLIELRRGRIVLLDEIGLQKLSE

Sequences:

>Translated_237_residues
MGTLQSDRLDDKVRYLRDIDIFRDLDPDEIELLGKRAPMQRVPAGTVFFSPEQRAEVLFILKEGRVRIYRLSTDGKMLTT
AILNEGTIFGEMALLGQQLHQHYAEALTPCLLCLMSREDVKRLLLSDPRIATRIVEILGQRLISAEQRLSDFAFKSLPQR
IATLLLQMAQPAKPRLFRPGSSQREVALTHEALAEMLGTYRETTTKILNDLRTQGLIELRRGRIVLLDEIGLQKLSE
>Mature_236_residues
GTLQSDRLDDKVRYLRDIDIFRDLDPDEIELLGKRAPMQRVPAGTVFFSPEQRAEVLFILKEGRVRIYRLSTDGKMLTTA
ILNEGTIFGEMALLGQQLHQHYAEALTPCLLCLMSREDVKRLLLSDPRIATRIVEILGQRLISAEQRLSDFAFKSLPQRI
ATLLLQMAQPAKPRLFRPGSSQREVALTHEALAEMLGTYRETTTKILNDLRTQGLIELRRGRIVLLDEIGLQKLSE

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=203, Percent_Identity=26.1083743842365, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27011; Mature: 26880

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLQSDRLDDKVRYLRDIDIFRDLDPDEIELLGKRAPMQRVPAGTVFFSPEQRAEVLFI
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCEEEECCCCCCEEEEE
LKEGRVRIYRLSTDGKMLTTAILNEGTIFGEMALLGQQLHQHYAEALTPCLLCLMSREDV
EECCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
KRLLLSDPRIATRIVEILGQRLISAEQRLSDFAFKSLPQRIATLLLQMAQPAKPRLFRPG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SSQREVALTHEALAEMLGTYRETTTKILNDLRTQGLIELRRGRIVLLDEIGLQKLSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCC
>Mature Secondary Structure 
GTLQSDRLDDKVRYLRDIDIFRDLDPDEIELLGKRAPMQRVPAGTVFFSPEQRAEVLFI
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCEEEECCCCCCEEEEE
LKEGRVRIYRLSTDGKMLTTAILNEGTIFGEMALLGQQLHQHYAEALTPCLLCLMSREDV
EECCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
KRLLLSDPRIATRIVEILGQRLISAEQRLSDFAFKSLPQRIATLLLQMAQPAKPRLFRPG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SSQREVALTHEALAEMLGTYRETTTKILNDLRTQGLIELRRGRIVLLDEIGLQKLSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630321 [H]