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The map label for this gene is dnaK [H]

Identifier: 222523592

GI number: 222523592

Start: 360963

End: 362312

Strand: Direct

Name: dnaK [H]

Synonym: Chy400_0298

Alternate gene names: 222523592

Gene position: 360963-362312 (Clockwise)

Preceding gene: 222523588

Following gene: 222523593

Centisome position: 6.85

GC content: 56.22

Gene sequence:

>1350_bases
ATGCGCGTCGGGCTTGACTTCGGCACGACTAACTCAAGCGCAGCGGTATATGACGGTCAGCGTTTGCGTTTGATCGATCT
CGATCCGGTGAATACACAACCGGCAATTCTCCGCTCGACGCTTTTCATCACCCGCGAAGGCGTACCGTTCATCGGGCGTG
AGGCGATCAATCGCTTCACCGAAGGGAATGTTGGGCGCGAGATTGAATACGTCTGGCGGTACATCGGCGATGCCGAACTG
ACCTTTGCCGAAAGCGGTACGGTGATGCAGGCATTGTACGTAAAGGTTGACGCCAATGCCCCAGGACGACTGTTTCAGTC
GTTGAAGAGTCATCTCCGTGATCGTAGCTTCCAGAAGACAAATGTGTTTGGCGTCTACTATACGCTGGAAGAGTTGATTG
CACTGGTCTTACGCATGATCATCGAGCGGATCGAGCAACAGCTTGGCGCTCCGATCAGTCATCTGGTAATTGGCCGACCG
GTGCATTACGCCAGCGATCCGGCCAGTGATGCGCTGGCTTTTGAGCGCATGCAGGCTGCATGTCGGCTGGCCGGCTTGCA
ATCCTTCAGTTTTCTGGAAGAACCGACCGCAGCAGCACTCTCGTATGCGCGCACAAACCAACGGGCCCAGCGCGTGCTGG
TGTTCGATTTTGGTGGTGGTACCCTTGACATCACGATTATGGAGCTTGATGAACGGGGCCGGCCTTCGTTCCTGGCAACC
GATGGTGTGCCGGTCGGTGGTGATTTGCTCGACCGCCGAATTGTGATGGGGCGGCTGTTACGTCATTTCGGCGAGGGCGC
TACGCTGGGAGCACGACGATTGCCGTTTCCCAATCACGTGTTGGAACACCTCAGTGAATGGCAAACGATCATCGATCTCA
CCCAACCTAAATATCTGGCGATTATTGATGAAGCGGTGGCGATTAGCGACCGGCCACGCGAGTTGCAGGCATTACGTACT
CTGGTACGCAAAAACTATGGGTTGCCGATGTACGAAGCGGTTGAGCGCACCAAAGTAGCGTTGTCGCAGGCGGATCGAGC
AACGTTTGAACTTGATATGGGTGAGATCAAGGTACGCGATGAGATTCCGCGCTGGGACTTCGAGCGGCTGATCGGCCCTG
ATGTGCGGGCGGTTGAGGCGTGTATTGATCGCGCTCTGAAAACTGCCGGCCTGCGCCCAGAACAGATTGATGTCGTTCTC
CGCACCGGTGGTAGCTCACGTGTCCCGCGTTTCGTGCGTATGCTGAGCGAGAAATTCGGCGCCGGGAAGTTGCAGGAGAT
CGATGTGTTCACCAGTGTTGCGGCAGGGCTGGCATTGAAGGCGAGCGAATATGACGCGGTAACCGGGTAA

Upstream 100 bases:

>100_bases
CCAGCATCGCCTGTCTACTTTCCATCGAAAGACTGATTGACAAAATCGATGTTGCCGGTTAGTCTGAAGTTTCAGTCATC
ACGATCAGGGAGGTACAACA

Downstream 100 bases:

>100_bases
TGGTACTCTTGGTCACTTTTCACCCCCTTGCGGTAAGTTGTTACGCGCCGTGGGTAGCAGTCTGTCTGGTAAACACACAC
CACGAACGCTGCTACCAGAT

Product: molecular chaperone-like protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRVGLDFGTTNSSAAVYDGQRLRLIDLDPVNTQPAILRSTLFITREGVPFIGREAINRFTEGNVGREIEYVWRYIGDAEL
TFAESGTVMQALYVKVDANAPGRLFQSLKSHLRDRSFQKTNVFGVYYTLEELIALVLRMIIERIEQQLGAPISHLVIGRP
VHYASDPASDALAFERMQAACRLAGLQSFSFLEEPTAAALSYARTNQRAQRVLVFDFGGGTLDITIMELDERGRPSFLAT
DGVPVGGDLLDRRIVMGRLLRHFGEGATLGARRLPFPNHVLEHLSEWQTIIDLTQPKYLAIIDEAVAISDRPRELQALRT
LVRKNYGLPMYEAVERTKVALSQADRATFELDMGEIKVRDEIPRWDFERLIGPDVRAVEACIDRALKTAGLRPEQIDVVL
RTGGSSRVPRFVRMLSEKFGAGKLQEIDVFTSVAAGLALKASEYDAVTG

Sequences:

>Translated_449_residues
MRVGLDFGTTNSSAAVYDGQRLRLIDLDPVNTQPAILRSTLFITREGVPFIGREAINRFTEGNVGREIEYVWRYIGDAEL
TFAESGTVMQALYVKVDANAPGRLFQSLKSHLRDRSFQKTNVFGVYYTLEELIALVLRMIIERIEQQLGAPISHLVIGRP
VHYASDPASDALAFERMQAACRLAGLQSFSFLEEPTAAALSYARTNQRAQRVLVFDFGGGTLDITIMELDERGRPSFLAT
DGVPVGGDLLDRRIVMGRLLRHFGEGATLGARRLPFPNHVLEHLSEWQTIIDLTQPKYLAIIDEAVAISDRPRELQALRT
LVRKNYGLPMYEAVERTKVALSQADRATFELDMGEIKVRDEIPRWDFERLIGPDVRAVEACIDRALKTAGLRPEQIDVVL
RTGGSSRVPRFVRMLSEKFGAGKLQEIDVFTSVAAGLALKASEYDAVTG
>Mature_449_residues
MRVGLDFGTTNSSAAVYDGQRLRLIDLDPVNTQPAILRSTLFITREGVPFIGREAINRFTEGNVGREIEYVWRYIGDAEL
TFAESGTVMQALYVKVDANAPGRLFQSLKSHLRDRSFQKTNVFGVYYTLEELIALVLRMIIERIEQQLGAPISHLVIGRP
VHYASDPASDALAFERMQAACRLAGLQSFSFLEEPTAAALSYARTNQRAQRVLVFDFGGGTLDITIMELDERGRPSFLAT
DGVPVGGDLLDRRIVMGRLLRHFGEGATLGARRLPFPNHVLEHLSEWQTIIDLTQPKYLAIIDEAVAISDRPRELQALRT
LVRKNYGLPMYEAVERTKVALSQADRATFELDMGEIKVRDEIPRWDFERLIGPDVRAVEACIDRALKTAGLRPEQIDVVL
RTGGSSRVPRFVRMLSEKFGAGKLQEIDVFTSVAAGLALKASEYDAVTG

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI34419635, Length=464, Percent_Identity=26.7241379310345, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI167466173, Length=450, Percent_Identity=26.8888888888889, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI194248072, Length=450, Percent_Identity=26.8888888888889, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI124256496, Length=462, Percent_Identity=26.4069264069264, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI16507237, Length=448, Percent_Identity=25.4464285714286, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI87082035, Length=456, Percent_Identity=25.8771929824561, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786196, Length=450, Percent_Identity=25.7777777777778, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786870, Length=102, Percent_Identity=38.2352941176471, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17534015, Length=322, Percent_Identity=28.8819875776398, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534013, Length=322, Percent_Identity=28.8819875776398, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17534771, Length=447, Percent_Identity=25.7270693512304, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568549, Length=457, Percent_Identity=25.382932166302, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71984444, Length=451, Percent_Identity=23.7250554323725, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319396, Length=447, Percent_Identity=26.1744966442953, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323004, Length=461, Percent_Identity=25.8134490238612, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6322505, Length=452, Percent_Identity=24.5575221238938, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320950, Length=322, Percent_Identity=27.3291925465839, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320805, Length=448, Percent_Identity=23.2142857142857, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17738165, Length=323, Percent_Identity=28.7925696594427, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17737941, Length=462, Percent_Identity=26.6233766233766, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24646324, Length=322, Percent_Identity=29.5031055900621, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24646322, Length=322, Percent_Identity=29.5031055900621, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24646318, Length=322, Percent_Identity=29.5031055900621, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28571678, Length=322, Percent_Identity=29.5031055900621, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24646137, Length=322, Percent_Identity=29.1925465838509, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24646135, Length=322, Percent_Identity=29.1925465838509, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24641406, Length=445, Percent_Identity=25.8426966292135, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641408, Length=445, Percent_Identity=25.8426966292135, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641402, Length=445, Percent_Identity=25.8426966292135, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641404, Length=445, Percent_Identity=25.8426966292135, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 50059; Mature: 50059

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGLDFGTTNSSAAVYDGQRLRLIDLDPVNTQPAILRSTLFITREGVPFIGREAINRFT
CEEECCCCCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHEEEECCCCCCCHHHHHHHC
EGNVGREIEYVWRYIGDAELTFAESGTVMQALYVKVDANAPGRLFQSLKSHLRDRSFQKT
CCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
NVFGVYYTLEELIALVLRMIIERIEQQLGAPISHLVIGRPVHYASDPASDALAFERMQAA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
CRLAGLQSFSFLEEPTAAALSYARTNQRAQRVLVFDFGGGTLDITIMELDERGRPSFLAT
HHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEECCCCCCCEEEC
DGVPVGGDLLDRRIVMGRLLRHFGEGATLGARRLPFPNHVLEHLSEWQTIIDLTQPKYLA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
IIDEAVAISDRPRELQALRTLVRKNYGLPMYEAVERTKVALSQADRATFELDMGEIKVRD
EEHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
EIPRWDFERLIGPDVRAVEACIDRALKTAGLRPEQIDVVLRTGGSSRVPRFVRMLSEKFG
CCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHCC
AGKLQEIDVFTSVAAGLALKASEYDAVTG
CCCCHHHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MRVGLDFGTTNSSAAVYDGQRLRLIDLDPVNTQPAILRSTLFITREGVPFIGREAINRFT
CEEECCCCCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHEEEECCCCCCCHHHHHHHC
EGNVGREIEYVWRYIGDAELTFAESGTVMQALYVKVDANAPGRLFQSLKSHLRDRSFQKT
CCCCCHHHHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
NVFGVYYTLEELIALVLRMIIERIEQQLGAPISHLVIGRPVHYASDPASDALAFERMQAA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
CRLAGLQSFSFLEEPTAAALSYARTNQRAQRVLVFDFGGGTLDITIMELDERGRPSFLAT
HHHHCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEECCCCCCCEEEC
DGVPVGGDLLDRRIVMGRLLRHFGEGATLGARRLPFPNHVLEHLSEWQTIIDLTQPKYLA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
IIDEAVAISDRPRELQALRTLVRKNYGLPMYEAVERTKVALSQADRATFELDMGEIKVRD
EEHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
EIPRWDFERLIGPDVRAVEACIDRALKTAGLRPEQIDVVLRTGGSSRVPRFVRMLSEKFG
CCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCCHHHHHHHHHHHCC
AGKLQEIDVFTSVAAGLALKASEYDAVTG
CCCCHHHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA