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The map label for this gene is troA [H]

Identifier: 222523504

GI number: 222523504

Start: 249982

End: 250899

Strand: Reverse

Name: troA [H]

Synonym: Chy400_0210

Alternate gene names: 222523504

Gene position: 250899-249982 (Counterclockwise)

Preceding gene: 222523505

Following gene: 222523503

Centisome position: 4.76

GC content: 55.66

Gene sequence:

>918_bases
ATGATTCTTCGATGTTCCTTAATCACTATTTTACTACTCATCCTAACCGGCTGTACAGCACCGGTTACTGATGGCCGTCT
GCGAGTCGTTGCTACCACCGGCCCGATAGGCGATGCAGTACAGGCGATTGCCGGTGATCGGGTGACGCTCCACACGCTGC
TTGGTCCTGGCATCGATCCCCATACCTACATTGCCACCGAGAGTGATCTGTTTGCTTTGCAAGAAGCGCGTCTGATCCTC
TACAACGGCCTTCATCTGGAAGCGAACCTTGAGCGGGTTTTTCAAGCTATTAAACGCAGTGGAACGATCCCGGTCGTTGC
AGTCGCTGAAGCTGTACCGGTAGAGATGCGTTTGCGCTGGGAAGATGATGAGAAGGCGTATGATCCGCATGTCTGGCACG
ATCCACAGCGCTGGCGCTATGCGGTCATTGCGATCCGTGATGCGTTGATTGATCTTGATCCGGACGGTCGGGAAGTTTAT
CAGCAGCGTAGTGAGCAGTATCTCAACGATTTACAGGCGCTTGACGCCGATCTGCGTGCGCTGGCCGAACAGGTACCTCC
TGAACGGCGGATTCTGGTGACGGCTCATGATGCGTTTAACTACTTTGGGCAGGCTTACGGTTTTCGGGTTGAGGCTGTGC
AGGGTATCAGTACGGCCAGCGAAGCCAGTGCCGCAACCATCCGTGATCTCACGACGCTCGTGGTAACGGAACGCATCCCG
GCGATCTTTGTCGAGACGAGTGTCTCGCCGCGCACAATTGAAGCGGTTCAGAGTGCTGCCCGTGCAGCCGGCCACGAGGT
GCGGGTCGGTGGTGAGTTGTATTCTGACTCACTCGGTGATGCTAATGGCCCGGCAGGTACCTATTTCGGCATGATGCGCC
ACAATATGCAGACGATTGTGGCTGCGCTCATGCCCTAA

Upstream 100 bases:

>100_bases
ATTGTTGCGTCTTATAGAGAGACTCTTGTCCACCCTTGACAATCTATCTCGTATCTGGTAGATTTAGGTAAGGCTAAAAT
TTATTTTAGGGTTAGTACAT

Downstream 100 bases:

>100_bases
AGCGGAGGGTGCAATGGAAGTTGCAGTTGACATCAAAGATTTGACCGTTGCCTACGGTGATAAACCGGTTTTATGGGACG
TTGATCTTCAGGTTCCCCGT

Product: periplasmic solute binding protein

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: Tromp-1 [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MILRCSLITILLLILTGCTAPVTDGRLRVVATTGPIGDAVQAIAGDRVTLHTLLGPGIDPHTYIATESDLFALQEARLIL
YNGLHLEANLERVFQAIKRSGTIPVVAVAEAVPVEMRLRWEDDEKAYDPHVWHDPQRWRYAVIAIRDALIDLDPDGREVY
QQRSEQYLNDLQALDADLRALAEQVPPERRILVTAHDAFNYFGQAYGFRVEAVQGISTASEASAATIRDLTTLVVTERIP
AIFVETSVSPRTIEAVQSAARAAGHEVRVGGELYSDSLGDANGPAGTYFGMMRHNMQTIVAALMP

Sequences:

>Translated_305_residues
MILRCSLITILLLILTGCTAPVTDGRLRVVATTGPIGDAVQAIAGDRVTLHTLLGPGIDPHTYIATESDLFALQEARLIL
YNGLHLEANLERVFQAIKRSGTIPVVAVAEAVPVEMRLRWEDDEKAYDPHVWHDPQRWRYAVIAIRDALIDLDPDGREVY
QQRSEQYLNDLQALDADLRALAEQVPPERRILVTAHDAFNYFGQAYGFRVEAVQGISTASEASAATIRDLTTLVVTERIP
AIFVETSVSPRTIEAVQSAARAAGHEVRVGGELYSDSLGDANGPAGTYFGMMRHNMQTIVAALMP
>Mature_305_residues
MILRCSLITILLLILTGCTAPVTDGRLRVVATTGPIGDAVQAIAGDRVTLHTLLGPGIDPHTYIATESDLFALQEARLIL
YNGLHLEANLERVFQAIKRSGTIPVVAVAEAVPVEMRLRWEDDEKAYDPHVWHDPQRWRYAVIAIRDALIDLDPDGREVY
QQRSEQYLNDLQALDADLRALAEQVPPERRILVTAHDAFNYFGQAYGFRVEAVQGISTASEASAATIRDLTTLVVTERIP
AIFVETSVSPRTIEAVQSAARAAGHEVRVGGELYSDSLGDANGPAGTYFGMMRHNMQTIVAALMP

Specific function: Part of an ATP-driven transport system troABCD for zinc. Substrate-binding protein involved in the transport of zinc across the cytoplasmic membrane [H]

COG id: COG0803

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface adhesin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 9 family [H]

Homologues:

Organism=Escherichia coli, GI87081990, Length=258, Percent_Identity=22.8682170542636, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006127
- InterPro:   IPR006129
- InterPro:   IPR006128 [H]

Pfam domain/function: PF01297 SBP_bac_9 [H]

EC number: NA

Molecular weight: Translated: 33440; Mature: 33440

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILRCSLITILLLILTGCTAPVTDGRLRVVATTGPIGDAVQAIAGDRVTLHTLLGPGIDP
CEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCEEEEEHHCCCCCC
HTYIATESDLFALQEARLILYNGLHLEANLERVFQAIKRSGTIPVVAVAEAVPVEMRLRW
CEEEECCCHHHHHHHCEEEEEECEEEEHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEE
EDDEKAYDPHVWHDPQRWRYAVIAIRDALIDLDPDGREVYQQRSEQYLNDLQALDADLRA
CCCCCCCCCCCCCCCCHHHEEEEEEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAEQVPPERRILVTAHDAFNYFGQAYGFRVEAVQGISTASEASAATIRDLTTLVVTERIP
HHHHCCCCCEEEEEEHHHHHHHHHHHCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
AIFVETSVSPRTIEAVQSAARAAGHEVRVGGELYSDSLGDANGPAGTYFGMMRHNMQTIV
EEEEECCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
AALMP
HHHCC
>Mature Secondary Structure
MILRCSLITILLLILTGCTAPVTDGRLRVVATTGPIGDAVQAIAGDRVTLHTLLGPGIDP
CEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCEEEEEHHCCCCCC
HTYIATESDLFALQEARLILYNGLHLEANLERVFQAIKRSGTIPVVAVAEAVPVEMRLRW
CEEEECCCHHHHHHHCEEEEEECEEEEHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEE
EDDEKAYDPHVWHDPQRWRYAVIAIRDALIDLDPDGREVYQQRSEQYLNDLQALDADLRA
CCCCCCCCCCCCCCCCHHHEEEEEEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAEQVPPERRILVTAHDAFNYFGQAYGFRVEAVQGISTASEASAATIRDLTTLVVTERIP
HHHHCCCCCEEEEEEHHHHHHHHHHHCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
AIFVETSVSPRTIEAVQSAARAAGHEVRVGGELYSDSLGDANGPAGTYFGMMRHNMQTIV
EEEEECCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
AALMP
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768866; 9332349; 9665876; 10400603; 10404217 [H]