Click here to switch to the map view.

The map label for this gene is gacS [H]

Identifier: 222523498

GI number: 222523498

Start: 241587

End: 243875

Strand: Direct

Name: gacS [H]

Synonym: Chy400_0204

Alternate gene names: 222523498

Gene position: 241587-243875 (Clockwise)

Preceding gene: 222523491

Following gene: 222523510

Centisome position: 4.59

GC content: 55.57

Gene sequence:

>2289_bases
ATGGCGGCTCTTCCCTTCGTGATGATCTATCTCATCCCGGCAATTATTGCCACCGGGCTGGCGATTGTCATGTGGCAACG
TCGTTACTATCGCGGAGGACGCCCCTTCACGCTCCTGCTGGCAGCGATTGCCTGGTGGAATCTCTGCCATGCGTTGAGCA
TCGCCGATACGACATTTGAAGGGACGCTCTTCTGGTCATTACTCCAAAATGTGGGCATTGTGCTGATTGGGCCATGCTGG
CTCTTAATCGCACTGGCATACTCTGGCGAATGGTGGCGCGTTGATCGCCGGATTCGGCGCGGTATTTTTATCCCCGAATT
GATTGCACTGATCTTCGCCCTTACCAACAACCTGCACTACCTTTGGTGGTCTACGGTTGTTGCCGATACGTCACGACCAT
TCCTCTGGCTCTCGGTCACCCGTGGGCCGGCCTTCTGGTTTCACAGCATCTACGCCTACGTCTGTCTGGCAATTGGCATT
ATCATCCTCTTCCGGGCCTCGGCACATGCACCACCGGTACACCGCTACCATTCCCGGCTGATGCTGGCAGCGGCCCTCAT
CCCGGCAGTCGGGAACATCGCCTTTCTCAGCGGCCTCAAGGTGCCGTGGAATGACGATCCGACACCGATTCTGCTCCTCA
TTTCCGCAGCCCTGGCGATGTACACGACAATGCGCTATCAGCTTTACGATCTGGCACCACTGGCCGAACAGGAGGTGATT
GCCGGGCTGCCCGATGGCTTAATTGTGCTCGACAGCCGTGGCCTGGTGGCTGAAATCAACCAGCACGCGCCACGACTATT
GGGTATCCAACAGGGGCGCTGGATCGGCAGGTCCTTAACCAGCCTGATCGTCAACTCGCCGTTTGAACGGGATTTGCAGC
GGTTACTCAACACGCCGGTTACCGGCCCTTCCCGGCCTGTCATCTGCGAAAATGGCACACAGGCGATTGAAGTGCGCCTC
CGCCCACTCCAATCGGCGGCTGGAATTACCACCGGTTCATTGCTTTTGATCCGCGATGTCAGTGAGCGTATGCGTGCCGA
ACAAGAGCGCGCACGCCACATTGCAGCCCTGCGTCTCATCAACAGCATCGCCCGCAGCGCCAACACCGCCCAAGAGACAT
CCACCCTGCTTAAGACGATTGCGCACATTATCGTGCAGGAAGGTCATTGGGAACGGGTATCGATTGGTCTGATCGATAAC
GCGCAGAACATCCATGTGACGATTGATTACACAACGGCAGGCTATGGACGATTACACGATCAGGTAATCAGCGGGGTCGC
CGCCAGTGCCCTGATCGAGCGTATTCAACATGGCCGACCCGAACTGATCAATTTGAGTGAACTCACTGTCACCGATCCAC
TGGCTGAAACCATGCAGCAAGAAGGGCTGCAATCGTTATTACTGGCCCCCCTGCGCCACGATCAACATGCGGCAGGCATT
TTGGGCCTGGCTTCGAGTGAAACTAAGAGCATGACGCCAACCCTTACCGAGCTGGTGATTGCCATTGGCGAATTGATTAC
CGATGCACTCATCCGGATTCGGCTCTACGAAGAGGTGCAACGTGCGGATCGGCTCAAAACCGGCTTTCTGGCCACCGTCA
GTCATGAACTACGTACTCCCCTTACCACCATCATCGGCTACACCGATATGCTACGCCGTGGTGTCCTGGGTGAATTAAGC
CCGGAAGTCCAGGAGACATTGCATTATATGCGCCAGGCCAGTCTCAATCTGATGCGCCTGATTAGCGATATTCTTGAGTT
CTCGCGGATGGAAGCCGGTCAGTTGATCATCGATATCGAACCGGTACCGGTTTCGGTTATCGTTCAGAATGTGGTTGGTC
AGATGCAACCGCAGATCCGCGAACATCAGCTTCATCTGATGGTCAATATTCCACCTGACTTACCAATGATTCAAGCCAAT
GCGGGTCGTCTCGAACAGGTTTTGATCAATCTTCTGAGTAATGCGATCAAGTTCAATCGTCCGCAAGGACTGATTGAGAT
CAAAGCCGAGACTCGTGGTGAGTGGGTACGCATCAGCGTGCGCGACACCGGCATTGGAATTGCCCCTGCCGATCAGGAAC
GGATTTTCGAGGAGTTTGAGCGCGTCAAGCAAAAGAATGGACATTCAGTCAGTGGCGTTGGTCTGGGGCTGGCGATCTGT
CGGCGGCTGATCCAGCACATGGGCGGCAAGATCGGGGTCAAGAGTACACCCGGCGAAGGATCGACCTTCTTTTGCGACCT
GCCAGTTGTGCCGGTATCGCGTACTGTTGGGGCAGAGGCCGACGTATGA

Upstream 100 bases:

>100_bases
TTGCCGGAAAGGGCAGTTCTTCGAGCACAGGTGATGAAGAGCAGAGGTGTGCCCTCTGCACCACACATACGCGAGAGAAC
TCTCGATTTGGAGCACAGGC

Downstream 100 bases:

>100_bases
CCGTACCCCCGATTCTGTTCTAAGCCGTCATCTCTCTCGCTGCCGGGCAGCGGCCACTCCTAACGCACCGGGCGTTACCC
GGATTGTCGTCGTGGCGGCG

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MAALPFVMIYLIPAIIATGLAIVMWQRRYYRGGRPFTLLLAAIAWWNLCHALSIADTTFEGTLFWSLLQNVGIVLIGPCW
LLIALAYSGEWWRVDRRIRRGIFIPELIALIFALTNNLHYLWWSTVVADTSRPFLWLSVTRGPAFWFHSIYAYVCLAIGI
IILFRASAHAPPVHRYHSRLMLAAALIPAVGNIAFLSGLKVPWNDDPTPILLLISAALAMYTTMRYQLYDLAPLAEQEVI
AGLPDGLIVLDSRGLVAEINQHAPRLLGIQQGRWIGRSLTSLIVNSPFERDLQRLLNTPVTGPSRPVICENGTQAIEVRL
RPLQSAAGITTGSLLLIRDVSERMRAEQERARHIAALRLINSIARSANTAQETSTLLKTIAHIIVQEGHWERVSIGLIDN
AQNIHVTIDYTTAGYGRLHDQVISGVAASALIERIQHGRPELINLSELTVTDPLAETMQQEGLQSLLLAPLRHDQHAAGI
LGLASSETKSMTPTLTELVIAIGELITDALIRIRLYEEVQRADRLKTGFLATVSHELRTPLTTIIGYTDMLRRGVLGELS
PEVQETLHYMRQASLNLMRLISDILEFSRMEAGQLIIDIEPVPVSVIVQNVVGQMQPQIREHQLHLMVNIPPDLPMIQAN
AGRLEQVLINLLSNAIKFNRPQGLIEIKAETRGEWVRISVRDTGIGIAPADQERIFEEFERVKQKNGHSVSGVGLGLAIC
RRLIQHMGGKIGVKSTPGEGSTFFCDLPVVPVSRTVGAEADV

Sequences:

>Translated_762_residues
MAALPFVMIYLIPAIIATGLAIVMWQRRYYRGGRPFTLLLAAIAWWNLCHALSIADTTFEGTLFWSLLQNVGIVLIGPCW
LLIALAYSGEWWRVDRRIRRGIFIPELIALIFALTNNLHYLWWSTVVADTSRPFLWLSVTRGPAFWFHSIYAYVCLAIGI
IILFRASAHAPPVHRYHSRLMLAAALIPAVGNIAFLSGLKVPWNDDPTPILLLISAALAMYTTMRYQLYDLAPLAEQEVI
AGLPDGLIVLDSRGLVAEINQHAPRLLGIQQGRWIGRSLTSLIVNSPFERDLQRLLNTPVTGPSRPVICENGTQAIEVRL
RPLQSAAGITTGSLLLIRDVSERMRAEQERARHIAALRLINSIARSANTAQETSTLLKTIAHIIVQEGHWERVSIGLIDN
AQNIHVTIDYTTAGYGRLHDQVISGVAASALIERIQHGRPELINLSELTVTDPLAETMQQEGLQSLLLAPLRHDQHAAGI
LGLASSETKSMTPTLTELVIAIGELITDALIRIRLYEEVQRADRLKTGFLATVSHELRTPLTTIIGYTDMLRRGVLGELS
PEVQETLHYMRQASLNLMRLISDILEFSRMEAGQLIIDIEPVPVSVIVQNVVGQMQPQIREHQLHLMVNIPPDLPMIQAN
AGRLEQVLINLLSNAIKFNRPQGLIEIKAETRGEWVRISVRDTGIGIAPADQERIFEEFERVKQKNGHSVSGVGLGLAIC
RRLIQHMGGKIGVKSTPGEGSTFFCDLPVVPVSRTVGAEADV
>Mature_761_residues
AALPFVMIYLIPAIIATGLAIVMWQRRYYRGGRPFTLLLAAIAWWNLCHALSIADTTFEGTLFWSLLQNVGIVLIGPCWL
LIALAYSGEWWRVDRRIRRGIFIPELIALIFALTNNLHYLWWSTVVADTSRPFLWLSVTRGPAFWFHSIYAYVCLAIGII
ILFRASAHAPPVHRYHSRLMLAAALIPAVGNIAFLSGLKVPWNDDPTPILLLISAALAMYTTMRYQLYDLAPLAEQEVIA
GLPDGLIVLDSRGLVAEINQHAPRLLGIQQGRWIGRSLTSLIVNSPFERDLQRLLNTPVTGPSRPVICENGTQAIEVRLR
PLQSAAGITTGSLLLIRDVSERMRAEQERARHIAALRLINSIARSANTAQETSTLLKTIAHIIVQEGHWERVSIGLIDNA
QNIHVTIDYTTAGYGRLHDQVISGVAASALIERIQHGRPELINLSELTVTDPLAETMQQEGLQSLLLAPLRHDQHAAGIL
GLASSETKSMTPTLTELVIAIGELITDALIRIRLYEEVQRADRLKTGFLATVSHELRTPLTTIIGYTDMLRRGVLGELSP
EVQETLHYMRQASLNLMRLISDILEFSRMEAGQLIIDIEPVPVSVIVQNVVGQMQPQIREHQLHLMVNIPPDLPMIQANA
GRLEQVLINLLSNAIKFNRPQGLIEIKAETRGEWVRISVRDTGIGIAPADQERIFEEFERVKQKNGHSVSGVGLGLAICR
RLIQHMGGKIGVKSTPGEGSTFFCDLPVVPVSRTVGAEADV

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=38.7351778656126, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1789149, Length=233, Percent_Identity=38.1974248927039, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI48994928, Length=270, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786912, Length=286, Percent_Identity=30.0699300699301, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081816, Length=280, Percent_Identity=32.8571428571429, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788713, Length=246, Percent_Identity=29.2682926829268, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=27.3684210526316, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI87082128, Length=220, Percent_Identity=31.8181818181818, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1790436, Length=240, Percent_Identity=31.6666666666667, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1788549, Length=255, Percent_Identity=32.156862745098, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=31.25, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=225, Percent_Identity=29.7777777777778, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790861, Length=209, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=234, Percent_Identity=24.3589743589744, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789808, Length=281, Percent_Identity=24.1992882562278, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787894, Length=229, Percent_Identity=26.2008733624454, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84528; Mature: 84397

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALPFVMIYLIPAIIATGLAIVMWQRRYYRGGRPFTLLLAAIAWWNLCHALSIADTTFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GTLFWSLLQNVGIVLIGPCWLLIALAYSGEWWRVDRRIRRGIFIPELIALIFALTNNLHY
HHHHHHHHHHCCEEEEHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
LWWSTVVADTSRPFLWLSVTRGPAFWFHSIYAYVCLAIGIIILFRASAHAPPVHRYHSRL
EEHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MLAAALIPAVGNIAFLSGLKVPWNDDPTPILLLISAALAMYTTMRYQLYDLAPLAEQEVI
HHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AGLPDGLIVLDSRGLVAEINQHAPRLLGIQQGRWIGRSLTSLIVNSPFERDLQRLLNTPV
HCCCCCEEEEECCCCHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCC
TGPSRPVICENGTQAIEVRLRPLQSAAGITTGSLLLIRDVSERMRAEQERARHIAALRLI
CCCCCCEEECCCCEEEEEEEEHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
NSIARSANTAQETSTLLKTIAHIIVQEGHWERVSIGLIDNAQNIHVTIDYTTAGYGRLHD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEEEECCCCHHHHH
QVISGVAASALIERIQHGRPELINLSELTVTDPLAETMQQEGLQSLLLAPLRHDQHAAGI
HHHHHHHHHHHHHHHHCCCCCEEECCCCEECHHHHHHHHHHHHHHHHHHHHCCCCHHCCE
LGLASSETKSMTPTLTELVIAIGELITDALIRIRLYEEVQRADRLKTGFLATVSHELRTP
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
LTTIIGYTDMLRRGVLGELSPEVQETLHYMRQASLNLMRLISDILEFSRMEAGQLIIDIE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PVPVSVIVQNVVGQMQPQIREHQLHLMVNIPPDLPMIQANAGRLEQVLINLLSNAIKFNR
CCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
PQGLIEIKAETRGEWVRISVRDTGIGIAPADQERIFEEFERVKQKNGHSVSGVGLGLAIC
CCCEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHH
RRLIQHMGGKIGVKSTPGEGSTFFCDLPVVPVSRTVGAEADV
HHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AALPFVMIYLIPAIIATGLAIVMWQRRYYRGGRPFTLLLAAIAWWNLCHALSIADTTFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GTLFWSLLQNVGIVLIGPCWLLIALAYSGEWWRVDRRIRRGIFIPELIALIFALTNNLHY
HHHHHHHHHHCCEEEEHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
LWWSTVVADTSRPFLWLSVTRGPAFWFHSIYAYVCLAIGIIILFRASAHAPPVHRYHSRL
EEHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
MLAAALIPAVGNIAFLSGLKVPWNDDPTPILLLISAALAMYTTMRYQLYDLAPLAEQEVI
HHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AGLPDGLIVLDSRGLVAEINQHAPRLLGIQQGRWIGRSLTSLIVNSPFERDLQRLLNTPV
HCCCCCEEEEECCCCHHHHHHCCCHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCC
TGPSRPVICENGTQAIEVRLRPLQSAAGITTGSLLLIRDVSERMRAEQERARHIAALRLI
CCCCCCEEECCCCEEEEEEEEHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
NSIARSANTAQETSTLLKTIAHIIVQEGHWERVSIGLIDNAQNIHVTIDYTTAGYGRLHD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEEEECCCCHHHHH
QVISGVAASALIERIQHGRPELINLSELTVTDPLAETMQQEGLQSLLLAPLRHDQHAAGI
HHHHHHHHHHHHHHHHCCCCCEEECCCCEECHHHHHHHHHHHHHHHHHHHHCCCCHHCCE
LGLASSETKSMTPTLTELVIAIGELITDALIRIRLYEEVQRADRLKTGFLATVSHELRTP
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
LTTIIGYTDMLRRGVLGELSPEVQETLHYMRQASLNLMRLISDILEFSRMEAGQLIIDIE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PVPVSVIVQNVVGQMQPQIREHQLHLMVNIPPDLPMIQANAGRLEQVLINLLSNAIKFNR
CCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHCCC
PQGLIEIKAETRGEWVRISVRDTGIGIAPADQERIFEEFERVKQKNGHSVSGVGLGLAIC
CCCEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHH
RRLIQHMGGKIGVKSTPGEGSTFFCDLPVVPVSRTVGAEADV
HHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]