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The map label for this gene is pleD [H]

Identifier: 222523495

GI number: 222523495

Start: 237938

End: 238858

Strand: Reverse

Name: pleD [H]

Synonym: Chy400_0201

Alternate gene names: 222523495

Gene position: 238858-237938 (Counterclockwise)

Preceding gene: 222523496

Following gene: 222523494

Centisome position: 4.53

GC content: 57.22

Gene sequence:

>921_bases
ATGGCATCAGCCGTTTCGTTTCTACCGATTATCACGACTGATAACGCACTGATCACTCAACTCAGCGCCCGCCTGGGTTC
TGGCTGGCAGATAGTACCGGCTTCCACAGTGACACCCGGTCAACCTAACTTGATCGATCTGCGCGAGCAGATACCTGAAG
GGCTGGCAGCTTATGCCGAACAGGTACCACTGGTTGGAGTGGTGGTTGATACTGCTGGCGCTGCTCACGTATTGCAGATC
GGGGTGCATGAAGTATTGACCGTCGCCGAGTTACAGCCTTTGTGTCTGACGCGGGCCATCACGTCGGCAACCATCCGCTG
GCAGATTGATCAGACCCGTCGCCAACACCTGGCTGAAGCCCGTGCGCAGGTGCAGGCATTGACAGTGCGATTGCAGAACG
AGCAGATCCGCGATCCGCTCACCGGTCTGTATACCCGGCGTTATCTTAACGAGACGCTTGATCGCGAACTGCGTCGGGCC
GACCGTGAGCAGATTGTGCTGAGCGTTGCGCTCATCGATATTGACCAGCTCGAACAGGTGAACTGGCAGTTTGGTCGTGC
TCTCGGTGATGCCGTGCTCCAACATCTGGCCGGCTTGATTGGGCGTCAGGTGCGGGGTGGCGACATCTTTTGTCGGTATG
GCAGTGATGAATTACTTCTGGTGCTACCGCGTGTCATGCCGGAAACGGCGTTTCAACGTGCCGAGCAGTGGCGGCTGTCG
GTAGCCGAATCGATCATTCGCCCCGGTACCCTTGATCTTCAGGTGACAATTTCTATCGGATTAGCCTCGTTCCCGCGTGA
TGCACGAAATGCCGTCGAATTGATGAGTCGCGTCGGTGAAGCCTTGTACGCAGCAAAGGCGGCTGGTGGCAACTGTGTGC
GGCAATGGCCGGGTGCTGACAGGTTGTTGTCGCAAGCCTGA

Upstream 100 bases:

>100_bases
GCTTCACCATCCGTAGGAGCGGTTTCAAAACCCTCCCCTACTTGTCGGTCGTGGCGAGTTGTGCTATCGTTGTCATAGCT
CATAGACTGTGGGGCATTGT

Downstream 100 bases:

>100_bases
TGCCTGAAGACAGAAAAAGTGCTGTGCTGGTTGAGACCGGTCTTTGGCGCTTGTCTGAATAATGAATTATCCATGGGTAT
AGATAGACTCTTACACTGAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MASAVSFLPIITTDNALITQLSARLGSGWQIVPASTVTPGQPNLIDLREQIPEGLAAYAEQVPLVGVVVDTAGAAHVLQI
GVHEVLTVAELQPLCLTRAITSATIRWQIDQTRRQHLAEARAQVQALTVRLQNEQIRDPLTGLYTRRYLNETLDRELRRA
DREQIVLSVALIDIDQLEQVNWQFGRALGDAVLQHLAGLIGRQVRGGDIFCRYGSDELLLVLPRVMPETAFQRAEQWRLS
VAESIIRPGTLDLQVTISIGLASFPRDARNAVELMSRVGEALYAAKAAGGNCVRQWPGADRLLSQA

Sequences:

>Translated_306_residues
MASAVSFLPIITTDNALITQLSARLGSGWQIVPASTVTPGQPNLIDLREQIPEGLAAYAEQVPLVGVVVDTAGAAHVLQI
GVHEVLTVAELQPLCLTRAITSATIRWQIDQTRRQHLAEARAQVQALTVRLQNEQIRDPLTGLYTRRYLNETLDRELRRA
DREQIVLSVALIDIDQLEQVNWQFGRALGDAVLQHLAGLIGRQVRGGDIFCRYGSDELLLVLPRVMPETAFQRAEQWRLS
VAESIIRPGTLDLQVTISIGLASFPRDARNAVELMSRVGEALYAAKAAGGNCVRQWPGADRLLSQA
>Mature_305_residues
ASAVSFLPIITTDNALITQLSARLGSGWQIVPASTVTPGQPNLIDLREQIPEGLAAYAEQVPLVGVVVDTAGAAHVLQIG
VHEVLTVAELQPLCLTRAITSATIRWQIDQTRRQHLAEARAQVQALTVRLQNEQIRDPLTGLYTRRYLNETLDRELRRAD
REQIVLSVALIDIDQLEQVNWQFGRALGDAVLQHLAGLIGRQVRGGDIFCRYGSDELLLVLPRVMPETAFQRAEQWRLSV
AESIIRPGTLDLQVTISIGLASFPRDARNAVELMSRVGEALYAAKAAGGNCVRQWPGADRLLSQA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=33.1325301204819, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=29.4478527607362, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=30.952380952381, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=31.6091954022989, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=30, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=31.875, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33603; Mature: 33472

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAVSFLPIITTDNALITQLSARLGSGWQIVPASTVTPGQPNLIDLREQIPEGLAAYAE
CCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEHHHHHCHHHHHHHHH
QVPLVGVVVDTAGAAHVLQIGVHEVLTVAELQPLCLTRAITSATIRWQIDQTRRQHLAEA
HCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHH
RAQVQALTVRLQNEQIRDPLTGLYTRRYLNETLDRELRRADREQIVLSVALIDIDQLEQV
HHHHHHHHHEECCHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHH
NWQFGRALGDAVLQHLAGLIGRQVRGGDIFCRYGSDELLLVLPRVMPETAFQRAEQWRLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHH
VAESIIRPGTLDLQVTISIGLASFPRDARNAVELMSRVGEALYAAKAAGGNCVRQWPGAD
HHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
RLLSQA
HHHHCC
>Mature Secondary Structure 
ASAVSFLPIITTDNALITQLSARLGSGWQIVPASTVTPGQPNLIDLREQIPEGLAAYAE
CCCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEHHHHHCHHHHHHHHH
QVPLVGVVVDTAGAAHVLQIGVHEVLTVAELQPLCLTRAITSATIRWQIDQTRRQHLAEA
HCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHH
RAQVQALTVRLQNEQIRDPLTGLYTRRYLNETLDRELRRADREQIVLSVALIDIDQLEQV
HHHHHHHHHEECCHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHH
NWQFGRALGDAVLQHLAGLIGRQVRGGDIFCRYGSDELLLVLPRVMPETAFQRAEQWRLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHH
VAESIIRPGTLDLQVTISIGLASFPRDARNAVELMSRVGEALYAAKAAGGNCVRQWPGAD
HHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHH
RLLSQA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA