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The map label for this gene is lpd [H]

Identifier: 222523476

GI number: 222523476

Start: 210998

End: 212335

Strand: Reverse

Name: lpd [H]

Synonym: Chy400_0182

Alternate gene names: 222523476

Gene position: 212335-210998 (Counterclockwise)

Preceding gene: 222523477

Following gene: 222523460

Centisome position: 4.03

GC content: 62.33

Gene sequence:

>1338_bases
ATGAAGCAGATTGTCGTGATTGGCGGTGGGGCTGCCGGTGTGGAAGCAGCAGTTGCTGCCGCCCAGGGCAACACAAAGGT
AACACTGGTCAGCGAAGGGCCAATTGGCGGTCGTACCGGTTGGGATAGCCTGCTACCGAGCAAAGTATGGCTCCATGCCG
CTGAGGTTGCCGGTATCGCGACGGCGGCAGCCGGCGAGGGCGTGGCGGTTAACGGGGTGCAGGTCGATCCGACAGCCGTC
TTACAACGGATCAAACACGTTGCAACGCGATGGAGTGATCACGAGCAGCAGCGGTTGCAGGCGGCTGGCGTAACCGTTAT
GCAGGGGGTTGCCGCCTTCAGCAGTCCTCACGAATTGACAATCCAGACTCAGGCAGGCCAGCAGACGATAACCGCCGATG
TCATCATTATTGCGACCGGTTCTGTTCCCCGCTTCCCCCCGACGATGAAACCGGACGGGCAGCGGATCATCGCGCCGCGC
TTTGCCAGCCATCTCCATACCCTTCCCGACGACATTATCGTAATCGGTGGTGGCCCGACCGGCAGCGAATTTGCCTCGCT
CTTCAGTCGGCTGGGTGTCAAAGTGACCTGGCTGGTCGGTAGCCCTGGCGTTTTGCCGATGTTTGCCACCAACGCCCGTT
CAACCCTGGCCGCAGCGATGCAGGCACACGGTGTCGAGATACATCAGGTTGATGTCGAACGGGCCGAGCGCACGGAAGAG
GGGGTAAGCGTTCTCGCGGCAGATGGCACTACCTATACGGCGGCCATGGCATTCCTGGCGATTGGCCGCACACCCGATCT
GAGCCGGCTCAACCTCAGTGCCGCCGGCCTGACCGTTGAGGCGAATGGTCAGCTAACCGTTGACAACTACGGACGCACGC
CAGTCAACCACATCTTCGCGGTCGGCGATGCGGCGGGCGGGCCGATGCTGGCGAACCGTGCGCTGGCGCAGGCGTGGATC
GCCGGGCGCACCGCTGCCGATCTGCCGGCACCGGGGTATTGCCCGCACACCATCGTGCATGCCGTCTACACTGTGCCTGA
AGTTGCCCAGGTGGGACTGGTCATGGATACAGAGCTGTCACGTATCCGCGCTGAATATGGCTCGTCGCTCAAGTCCTACC
TTGGTGCAGAGACATCTGGCTGGATCGAGCTGACCTTTGATCCGATCACGCGCCAGGTTCGCGGTGGGGTAGCAGTGGGC
GATCATGCTGCCGATCTACTGGCCCCGGTTGCCCTGGCGATCCAGACCGGGGCTACCCTGGCCGATCTGGCAGCCGTCTT
TGCCGCCTATCCAACGTTAAGCGAGACTGTTTTTGCCGCAGCGCGCGCAGCAGGGTGA

Upstream 100 bases:

>100_bases
TTTCGGCGGCTGATTGCATCACCTTATATAGTGGGTGAATATACAGGTAATCACCGGACAGGTAGGAGCATTATCTTCAT
CGAACATACGGAGAGGCGTT

Downstream 100 bases:

>100_bases
TAGGCTGAGATCCTTATCAGACTGGTCACCGCGCACCGGTTACTTCAAGCCGGTGGAGAGTGGTACCCTTCCCAGCAGGG
GCGAGGCGCGGGAGAGAGGA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKQIVVIGGGAAGVEAAVAAAQGNTKVTLVSEGPIGGRTGWDSLLPSKVWLHAAEVAGIATAAAGEGVAVNGVQVDPTAV
LQRIKHVATRWSDHEQQRLQAAGVTVMQGVAAFSSPHELTIQTQAGQQTITADVIIIATGSVPRFPPTMKPDGQRIIAPR
FASHLHTLPDDIIVIGGGPTGSEFASLFSRLGVKVTWLVGSPGVLPMFATNARSTLAAAMQAHGVEIHQVDVERAERTEE
GVSVLAADGTTYTAAMAFLAIGRTPDLSRLNLSAAGLTVEANGQLTVDNYGRTPVNHIFAVGDAAGGPMLANRALAQAWI
AGRTAADLPAPGYCPHTIVHAVYTVPEVAQVGLVMDTELSRIRAEYGSSLKSYLGAETSGWIELTFDPITRQVRGGVAVG
DHAADLLAPVALAIQTGATLADLAAVFAAYPTLSETVFAAARAAG

Sequences:

>Translated_445_residues
MKQIVVIGGGAAGVEAAVAAAQGNTKVTLVSEGPIGGRTGWDSLLPSKVWLHAAEVAGIATAAAGEGVAVNGVQVDPTAV
LQRIKHVATRWSDHEQQRLQAAGVTVMQGVAAFSSPHELTIQTQAGQQTITADVIIIATGSVPRFPPTMKPDGQRIIAPR
FASHLHTLPDDIIVIGGGPTGSEFASLFSRLGVKVTWLVGSPGVLPMFATNARSTLAAAMQAHGVEIHQVDVERAERTEE
GVSVLAADGTTYTAAMAFLAIGRTPDLSRLNLSAAGLTVEANGQLTVDNYGRTPVNHIFAVGDAAGGPMLANRALAQAWI
AGRTAADLPAPGYCPHTIVHAVYTVPEVAQVGLVMDTELSRIRAEYGSSLKSYLGAETSGWIELTFDPITRQVRGGVAVG
DHAADLLAPVALAIQTGATLADLAAVFAAYPTLSETVFAAARAAG
>Mature_445_residues
MKQIVVIGGGAAGVEAAVAAAQGNTKVTLVSEGPIGGRTGWDSLLPSKVWLHAAEVAGIATAAAGEGVAVNGVQVDPTAV
LQRIKHVATRWSDHEQQRLQAAGVTVMQGVAAFSSPHELTIQTQAGQQTITADVIIIATGSVPRFPPTMKPDGQRIIAPR
FASHLHTLPDDIIVIGGGPTGSEFASLFSRLGVKVTWLVGSPGVLPMFATNARSTLAAAMQAHGVEIHQVDVERAERTEE
GVSVLAADGTTYTAAMAFLAIGRTPDLSRLNLSAAGLTVEANGQLTVDNYGRTPVNHIFAVGDAAGGPMLANRALAQAWI
AGRTAADLPAPGYCPHTIVHAVYTVPEVAQVGLVMDTELSRIRAEYGSSLKSYLGAETSGWIELTFDPITRQVRGGVAVG
DHAADLLAPVALAIQTGATLADLAAVFAAYPTLSETVFAAARAAG

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=27.1948608137045, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=25.4901960784314, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=26.8980477223427, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=28.9183222958057, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87082354, Length=453, Percent_Identity=26.0485651214128, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=26.9585253456221, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=24.3534482758621, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=479, Percent_Identity=29.0187891440501, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17557007, Length=485, Percent_Identity=22.680412371134, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983429, Length=431, Percent_Identity=22.737819025522, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71983419, Length=431, Percent_Identity=22.737819025522, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=470, Percent_Identity=27.6595744680851, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=25.9493670886076, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=24.1452991452991, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=26.8240343347639, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=25.531914893617, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=25.531914893617, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=25.531914893617, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=26.2396694214876, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 45856; Mature: 45856

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIVVIGGGAAGVEAAVAAAQGNTKVTLVSEGPIGGRTGWDSLLPSKVWLHAAEVAGIA
CCEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHH
TAAAGEGVAVNGVQVDPTAVLQRIKHVATRWSDHEQQRLQAAGVTVMQGVAAFSSPHELT
HHCCCCCEEECCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCCCEEE
IQTQAGQQTITADVIIIATGSVPRFPPTMKPDGQRIIAPRFASHLHTLPDDIIVIGGGPT
EEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEECHHHHHHHHCCCCCEEEECCCCC
GSEFASLFSRLGVKVTWLVGSPGVLPMFATNARSTLAAAMQAHGVEIHQVDVERAERTEE
HHHHHHHHHHCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHCCCEEEEECHHHHHHHHC
GVSVLAADGTTYTAAMAFLAIGRTPDLSRLNLSAAGLTVEANGQLTVDNYGRTPVNHIFA
CCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCEEEECCCCCCCHHHEEE
VGDAAGGPMLANRALAQAWIAGRTAADLPAPGYCPHTIVHAVYTVPEVAQVGLVMDTELS
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCEEECHHHH
RIRAEYGSSLKSYLGAETSGWIELTFDPITRQVRGGVAVGDHAADLLAPVALAIQTGATL
HHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCHH
ADLAAVFAAYPTLSETVFAAARAAG
HHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKQIVVIGGGAAGVEAAVAAAQGNTKVTLVSEGPIGGRTGWDSLLPSKVWLHAAEVAGIA
CCEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHH
TAAAGEGVAVNGVQVDPTAVLQRIKHVATRWSDHEQQRLQAAGVTVMQGVAAFSSPHELT
HHCCCCCEEECCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCCCEEE
IQTQAGQQTITADVIIIATGSVPRFPPTMKPDGQRIIAPRFASHLHTLPDDIIVIGGGPT
EEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEECHHHHHHHHCCCCCEEEECCCCC
GSEFASLFSRLGVKVTWLVGSPGVLPMFATNARSTLAAAMQAHGVEIHQVDVERAERTEE
HHHHHHHHHHCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHCCCEEEEECHHHHHHHHC
GVSVLAADGTTYTAAMAFLAIGRTPDLSRLNLSAAGLTVEANGQLTVDNYGRTPVNHIFA
CCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCEEEECCCCCCCHHHEEE
VGDAAGGPMLANRALAQAWIAGRTAADLPAPGYCPHTIVHAVYTVPEVAQVGLVMDTELS
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCEEECHHHH
RIRAEYGSSLKSYLGAETSGWIELTFDPITRQVRGGVAVGDHAADLLAPVALAIQTGATL
HHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCHH
ADLAAVFAAYPTLSETVFAAARAAG
HHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]