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The map label for this gene is manB [H]

Identifier: 222523349

GI number: 222523349

Start: 52140

End: 53483

Strand: Direct

Name: manB [H]

Synonym: Chy400_0050

Alternate gene names: 222523349

Gene position: 52140-53483 (Clockwise)

Preceding gene: 222523348

Following gene: 222523350

Centisome position: 0.99

GC content: 54.99

Gene sequence:

>1344_bases
GTGCAGGTTAATCCAACCATTTTCAAAGCCTACGATATTCGTGGCATTTATCCGACCGAGCTTAACGAAGAGGTCGCCTA
TTTGATTGGTCGGGCGTTCGTCACTTTTCTCGGTGCCGAAACGGTGATTGTTGGCCGGGATATGCGCACATCAGGGCCAG
CCCTGTTCGACGCCGTCACCCGTGGGATTATGGATCAGGGTGCCGATGTGGCCGATATTGGCATGGTCAGCACCGATCAG
TACTACTTTGCCTGTACGCAACTTGGCTTCCCCGGTATGATGGTGACGGCATCACACAACCCCAAACAGTACAATGGCTT
CAAGATGGTGCGCCGGATGCCGTACCTCTTGTCGGGTGATGAGGGTATTCAGGATTTGCGCCGGTTAGTCGAGAGTGAAG
CCTTTCCGACCCCAACCCGCCGTGGTCAGCGCCGGGAGTACGACTTCAAAGAGCAATTCGTGCAGAAGGTGCTCTCGTTG
ATTGATGTAGACGCTATCAAGCCGCTGAAAGTGATTGTTGACACCGGTAACGGGATGGTAGGGCCGATTTTGCAGGAGGT
GTATGCCCGCTTACCGATCCAATTGACCGGGATGTACCTCGATCCCGATGGTACGCTGCCCAATCACGGGCTTGATCCGC
TGATGCCGGAAAACCGCGCCGAATTGCAACAGCGTGTGAAAGATGAGGGAGCCGACATCGGCTTCGCATTCGACGGTGAC
GGTGATCGTTTCTTCGCGATTGATGATCGCGGTGAGTTTATTTCCGGTGATTTTCTCACGGCAATTCTGGGGCGTTACCT
GCTTGAGAAAGAACCAGGCGCAAAGATTCTCTACGACGTGCGGGCATCGTGGGCGGTACCCGATGAGGTGCGTGCTGCCG
GTGGTGTTCCGCTGGTCGAACGGGTCGGTCACGCCTTCATCAAGCGCCGCATGGCCAATGAAGACGCAATCTTTGCCGGT
GAAGTCTCCGGACATTACTACTTCAAAGCCTTCGCGTTCGCCGACTCAGGCATCATTCCATCACTCTACCTGCTCGAAAT
GCTCTCGAAGCGCGGGGTCAAGATGAGCGAACTGCTCGGTCGGCTGGAGTCGCGCTACTTCATTTCGGGTGAAATCAACT
CACGGGTCAGCGATGTAGCCGCCAAGCTCAATGAGATTGCCGAACGCTACAGTGATGGCAAAATCGAACGAATTGACGGC
GTTTCGGTGAGTTACGATACCTGGCACTTTAATGTGCGCGGGTCGAATACCGAACCGCTGATCCGGCTCAATCTCGAATC
GATTGCCTCACGCGAAGAGATGGAGGCAAAACGCGATGAGGTGCTGGCCGTCATTCGTTCGTGA

Upstream 100 bases:

>100_bases
TCTCACCATTCAACATCTCCGAGTGCTCTGTGTCTCTGTGGTGACACAACTCTACCTGTGTCTCTGTGGTGACAACACCT
TCTCAGTAAGGAGGAATATT

Downstream 100 bases:

>100_bases
TGGCTGGTTTGCCCGCACAGCATCGAAATATTATGGTCAGCCAACGAGATTTGCGCATAATGGGTTGACGCTAACTCGAT
ACAGGCAGGAGTCATCGGCA

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MQVNPTIFKAYDIRGIYPTELNEEVAYLIGRAFVTFLGAETVIVGRDMRTSGPALFDAVTRGIMDQGADVADIGMVSTDQ
YYFACTQLGFPGMMVTASHNPKQYNGFKMVRRMPYLLSGDEGIQDLRRLVESEAFPTPTRRGQRREYDFKEQFVQKVLSL
IDVDAIKPLKVIVDTGNGMVGPILQEVYARLPIQLTGMYLDPDGTLPNHGLDPLMPENRAELQQRVKDEGADIGFAFDGD
GDRFFAIDDRGEFISGDFLTAILGRYLLEKEPGAKILYDVRASWAVPDEVRAAGGVPLVERVGHAFIKRRMANEDAIFAG
EVSGHYYFKAFAFADSGIIPSLYLLEMLSKRGVKMSELLGRLESRYFISGEINSRVSDVAAKLNEIAERYSDGKIERIDG
VSVSYDTWHFNVRGSNTEPLIRLNLESIASREEMEAKRDEVLAVIRS

Sequences:

>Translated_447_residues
MQVNPTIFKAYDIRGIYPTELNEEVAYLIGRAFVTFLGAETVIVGRDMRTSGPALFDAVTRGIMDQGADVADIGMVSTDQ
YYFACTQLGFPGMMVTASHNPKQYNGFKMVRRMPYLLSGDEGIQDLRRLVESEAFPTPTRRGQRREYDFKEQFVQKVLSL
IDVDAIKPLKVIVDTGNGMVGPILQEVYARLPIQLTGMYLDPDGTLPNHGLDPLMPENRAELQQRVKDEGADIGFAFDGD
GDRFFAIDDRGEFISGDFLTAILGRYLLEKEPGAKILYDVRASWAVPDEVRAAGGVPLVERVGHAFIKRRMANEDAIFAG
EVSGHYYFKAFAFADSGIIPSLYLLEMLSKRGVKMSELLGRLESRYFISGEINSRVSDVAAKLNEIAERYSDGKIERIDG
VSVSYDTWHFNVRGSNTEPLIRLNLESIASREEMEAKRDEVLAVIRS
>Mature_447_residues
MQVNPTIFKAYDIRGIYPTELNEEVAYLIGRAFVTFLGAETVIVGRDMRTSGPALFDAVTRGIMDQGADVADIGMVSTDQ
YYFACTQLGFPGMMVTASHNPKQYNGFKMVRRMPYLLSGDEGIQDLRRLVESEAFPTPTRRGQRREYDFKEQFVQKVLSL
IDVDAIKPLKVIVDTGNGMVGPILQEVYARLPIQLTGMYLDPDGTLPNHGLDPLMPENRAELQQRVKDEGADIGFAFDGD
GDRFFAIDDRGEFISGDFLTAILGRYLLEKEPGAKILYDVRASWAVPDEVRAAGGVPLVERVGHAFIKRRMANEDAIFAG
EVSGHYYFKAFAFADSGIIPSLYLLEMLSKRGVKMSELLGRLESRYFISGEINSRVSDVAAKLNEIAERYSDGKIERIDG
VSVSYDTWHFNVRGSNTEPLIRLNLESIASREEMEAKRDEVLAVIRS

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=450, Percent_Identity=42.2222222222222, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1789566, Length=416, Percent_Identity=25.4807692307692, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 49981; Mature: 49981

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVNPTIFKAYDIRGIYPTELNEEVAYLIGRAFVTFLGAETVIVGRDMRTSGPALFDAVT
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHH
RGIMDQGADVADIGMVSTDQYYFACTQLGFPGMMVTASHNPKQYNGFKMVRRMPYLLSGD
HHHHHCCCCCHHCCEEECCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
EGIQDLRRLVESEAFPTPTRRGQRREYDFKEQFVQKVLSLIDVDAIKPLKVIVDTGNGMV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
GPILQEVYARLPIQLTGMYLDPDGTLPNHGLDPLMPENRAELQQRVKDEGADIGFAFDGD
HHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
GDRFFAIDDRGEFISGDFLTAILGRYLLEKEPGAKILYDVRASWAVPDEVRAAGGVPLVE
CCEEEEECCCCCEECHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCCHHHH
RVGHAFIKRRMANEDAIFAGEVSGHYYFKAFAFADSGIIPSLYLLEMLSKRGVKMSELLG
HHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHH
RLESRYFISGEINSRVSDVAAKLNEIAERYSDGKIERIDGVSVSYDTWHFNVRGSNTEPL
HHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEEEECCCCCCE
IRLNLESIASREEMEAKRDEVLAVIRS
EEEEHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVNPTIFKAYDIRGIYPTELNEEVAYLIGRAFVTFLGAETVIVGRDMRTSGPALFDAVT
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHH
RGIMDQGADVADIGMVSTDQYYFACTQLGFPGMMVTASHNPKQYNGFKMVRRMPYLLSGD
HHHHHCCCCCHHCCEEECCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
EGIQDLRRLVESEAFPTPTRRGQRREYDFKEQFVQKVLSLIDVDAIKPLKVIVDTGNGMV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
GPILQEVYARLPIQLTGMYLDPDGTLPNHGLDPLMPENRAELQQRVKDEGADIGFAFDGD
HHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
GDRFFAIDDRGEFISGDFLTAILGRYLLEKEPGAKILYDVRASWAVPDEVRAAGGVPLVE
CCEEEEECCCCCEECHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCCHHHH
RVGHAFIKRRMANEDAIFAGEVSGHYYFKAFAFADSGIIPSLYLLEMLSKRGVKMSELLG
HHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHH
RLESRYFISGEINSRVSDVAAKLNEIAERYSDGKIERIDGVSVSYDTWHFNVRGSNTEPL
HHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEEEEECCCCCCE
IRLNLESIASREEMEAKRDEVLAVIRS
EEEEHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]