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The map label for this gene is nrgA [H]

Identifier: 222094879

GI number: 222094879

Start: 1207086

End: 1208318

Strand: Reverse

Name: nrgA [H]

Synonym: BCQ_1217

Alternate gene names: 222094879

Gene position: 1208318-1207086 (Counterclockwise)

Preceding gene: 222094882

Following gene: 222094875

Centisome position: 23.17

GC content: 43.88

Gene sequence:

>1233_bases
ATGAATACAGGAGATACGGTTTTTATGTTTGTAGCGACAGTAATGGTCATGTTAATGACACCAGGATTAGCACTTTTTTA
TGGAGGTATGGTTCGCAGTAAAAACGTACTCAGTACGACAATGCATAGTTATAGCGCAATGGCTATCGTTTCGATTCAAT
GGATTGTAATTGGTTATTCTTTATCATTCGGACCAGATTGGCACGGACTTATCGGCACATTCGATTGGTTCGGTTTAAAC
GGAGTTAGCTACGCACCAAATCCAGACTACTCATCTACTATTCCTCACAATTTATTTATGATGTTTCAACTCATGTTTGC
TATTTTAACTCCCGCTCTAATTTCAGGAGCATTTGCCGAAAGAATGCGATTTTCAGCTTTCCTTATTTTCATCCTTCTGT
GGACAACAATCGTTTACAATCCTGTCGCTCATTGGGTATGGGGCGTTGGTGGATGGCTAAGAGAACTTGGCACATTAGAC
TTCGCTGGCGGTAATGTCGTTCATATTACATCTGGCGTGGCTGGCCTCGTATTAGCTATTTTCCTTGGAAAACGCAAAAA
CATAAACGGTTCGTCTCCTCATCATTTACCATTTACGATGTTAGGCGCTGGTTTACTGTGGTTCGGCTGGTTCGGTTTTA
ACGTCGGAAGCGCATTATCTTTAAACGACGTAGCTTTAACTGCATTTATTAATACAAATATAGCCGCCGCTGCCTCCGCA
TTAACTTGGATGCTTTCAGAGTGGTTCTTCCAATCAAAACCGACCGCGATGGGAGCTGCTTGCGGCGTTGTTTCCGGTCT
AGTCGCTATTACACCAGCTTGCGGGTTCGTTACACCTTTCTCAGCTCTTCTTATCGGAGCAATTGGCGGCGTATTATGCT
TCGGAGCGGTCTTTTTCTTAAAAACAAAATTCGGATACGACGATACACTCGATGCATTTGGATGTCACGGCATCGGCGGA
ACTTGGGGCGGCATTGCGACAGGACTATTTGCAACTACCACCGTAAACAGTGATGGCGCTAACGGATTATTTTACGGGAA
CGCCGCGCTGCTGTTGAAACAACTTGTAGCAATCGGCGCAACATATGCGTTCACAATTATAATGACGTACGCGATTATTA
AAGCAATTAACTTCTTCCTCCCTGTCCGGGTAGATGAGCACGAGGAACATATGGGACTTGATATTTCAATGCACGGGGAG
AAAGCTTATGAATATACTGAGCGAGTGAACTAA

Upstream 100 bases:

>100_bases
ATTTTTCTGAATTTTTAGTTGATTTTTCTTTTTATTTCGAATATGATATGAAACATAACAAATTGATGTTATTTTTCCTA
ACATTATAGGAGGAGAGAGA

Downstream 100 bases:

>100_bases
AAAAGAGATGCAATCTATTGATAGTAGATTGCATCTCTTTTTATTAGACTTGCCGGCACCATACCCTGAGCGGTATTAAT
ATAAATATATCAGCGATTCT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNTGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTFDWFGLN
GVSYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGTLD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGVVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNSDGANGLFYGNAALLLKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Sequences:

>Translated_410_residues
MNTGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTFDWFGLN
GVSYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGTLD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGVVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNSDGANGLFYGNAALLLKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN
>Mature_410_residues
MNTGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTFDWFGLN
GVSYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGTLD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGVVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNSDGANGLFYGNAALLLKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=42.2604422604423, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=29.5348837209302, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=28.2660332541568, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=26.9230769230769, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=442, Percent_Identity=28.5067873303167, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=414, Percent_Identity=40.0966183574879, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=35.6459330143541, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=36.1244019138756, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=30.2884615384615, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44144; Mature: 44144

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEEEEEEEE
LSFGPDWHGLIGTFDWFGLNGVSYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCCCHHHHCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGTLDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCHHHHHHHH
FLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGVVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTTVNSDGANGLFYGNAALLLKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHCCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MNTGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHEEEEEEEEEE
LSFGPDWHGLIGTFDWFGLNGVSYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCCCHHHHCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGTLDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCCHHHHHHHH
FLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGVVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTTVNSDGANGLFYGNAALLLKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHCCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]