Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 221232832

GI number: 221232832

Start: 2089965

End: 2091254

Strand: Reverse

Name: hisS

Synonym: SPN23F_21500

Alternate gene names: 221232832

Gene position: 2091254-2089965 (Counterclockwise)

Preceding gene: 221232833

Following gene: 221232831

Centisome position: 94.14

GC content: 47.13

Gene sequence:

>1290_bases
ATGAAATTACAAAAACCAAAAGGAACGCAGGATATTTTACCTGCTGAATCTGCCAAATGGCAGTACGTTGAGGGCTTTGC
CCGTGAAATTTTCAAGCGCTACAACTACGCAGAAGTGCGCACGCCCATTTTTGAGCATTACGAGGTTATCAGCCGCTCTG
TCGGAGATACGACCGATATCGTAACCAAGGAAATGTACGATTTCTATGACAAGGGTGACCGCCATATTACCCTCCGTCCA
GAAGGAACTGCGCCCGTTGTCCGTTCCTATGTGGAAAATAAACTCTTCGCCCCAGAAGTGCAAAAGCCAAGCAAGTTCTA
CTACATGGGACCTATGTTCCGTTATGAGCGTCCACAGGCAGGGCGCTTGCGCCAATTCCACCAGATTGGTGTTGAGTGTT
TTGGCTCTAGCAATCCAGCTACCGATGTGGAAACAATCGCTATGGCAGCCCATTTTTTGAAGGAAATCGGTATTCAAGGT
GTCAAATTGCACCTCAACACTCTTGGAAATCCTGAGAGCCGTGCAGCCTACCGCCAAGCCTTGATTGACTATTTGACACC
GCTCAAGGAGACCTTGTCTAAGGATAGCCAACGTCGCTTGGAGGAAAATCCTCTTCGTGTCTTGGACTCTAAGGAAAAAG
AAGACAAGGTGGCAGTAGAGAATGCGCCGTCTATCTTGGACTTTCTTGATGAAGAAAGCCAAACTCATTTTGATGCTGTG
CGTCAGATGTTGGAAAATCTTGGAGTAGATTACATCATCGATACCAATATGGTGCGTGGTCTGGACTACTACAACCACAC
CATTTTCGAGTTTATCACAGAGATTGAGGGCAATGACTTGACAGTCTGTGCGGGTGGTCGCTACGATGGTTTGGTTGCTT
ACTTTGGAGGCCCTGAAACTGCTGGATTTGGTTTTGGGCTTGGTGTAGAGCGCCTGCTTCTCATCCTTGAAAAACAAGGC
GTGGCCCTCCCTATCGAAAACGCCCTAGATGTCTATATCGCAGTCTTGGGTGATGGAGCAAATGTCAAAGCCCTAGAACT
AGTCCAAGCTCTTCGCCAACAAGGTTTCAAAGCAGAGCGTGATTACCTCAACCGTAAGCTCAAAGCTCAGTTCAAGTCAG
CAGATGTCTTTGCGGCTAAGACCCTCATCACCCTAGGAGAGAGCGAAGTCGAAAGCGGACAAGTGACGGTCAAGAACAAC
CAAACCCGAGAAGAAGTGCAAGTGTCACTTGAGACAATCAGCCAAAACTTCTCAGAAATCTTTGAAAAACTAGGATTTTA
TACTCAATGA

Upstream 100 bases:

>100_bases
TTAATCTCAAAAATGATATCTAAATCCTAATTTTTTTCTCACTGTTTTAATCCCTTTATTTGTGGTAAAATAAGACTATT
AAGTTTAAAGAGGATTCCCT

Downstream 100 bases:

>100_bases
AAATCAAAGAGCAAACTAGGAAGCTAGCCGCAGGTTGCTCAAAACACTGTTTTGAAGTTATGGATAGAACTGACGAAGTC
AGCTCAAAACATGGTTTTGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ

Sequences:

>Translated_429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ
>Mature_429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=478, Percent_Identity=25.7322175732218, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI6996014, Length=473, Percent_Identity=26.8498942917548, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=41.2322274881517, Blast_Score=355, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=26.9565217391304, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71993686, Length=460, Percent_Identity=26.9565217391304, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325290, Length=472, Percent_Identity=21.8220338983051, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24643061, Length=465, Percent_Identity=25.3763440860215, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24643059, Length=484, Percent_Identity=24.5867768595041, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_STRP2 (Q04I50)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817338.1
- ProteinModelPortal:   Q04I50
- SMR:   Q04I50
- STRING:   Q04I50
- EnsemblBacteria:   EBSTRT00000020584
- GeneID:   4441552
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1950
- eggNOG:   COG0124
- GeneTree:   EBGT00050000027423
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48627; Mature: 48627

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCH
GRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
LIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
HHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHH
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCC
AGFGFGLGVERLLLILEKQGVALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAER
CCCCCCHHHHHHHHHHHCCCCEECHHHHCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
DYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNNQTREEVQVSLETISQNFSEI
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
FEKLGFYTQ
HHHHCCCCC
>Mature Secondary Structure
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCH
GRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
LIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
HHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHH
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCC
AGFGFGLGVERLLLILEKQGVALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAER
CCCCCCHHHHHHHHHHHCCCCEECHHHHCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
DYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNNQTREEVQVSLETISQNFSEI
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
FEKLGFYTQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA