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The map label for this gene is dapL

Identifier: 221232804

GI number: 221232804

Start: 2059489

End: 2060619

Strand: Reverse

Name: dapL

Synonym: SPN23F_21200

Alternate gene names: 221232804

Gene position: 2060619-2059489 (Counterclockwise)

Preceding gene: 221232805

Following gene: 221232803

Centisome position: 92.77

GC content: 47.13

Gene sequence:

>1131_bases
ATGTTAGATTTGATTCAGACTAGACGAGATTTACACCAGATTCCAGAGATTGGCTTGGAGGAGTTCAAGACTCAGGCTTA
TTTGCTGGATGTGATTGAGAAATTGACTACGGGCAAGGATTTTGTTCAAATTCGTACTTGGCGGACAGGGATTTTGGTCT
ACCTGCAGGGAAGTCAGCCGGAGCGAACCATTGGTTGGCGGACAGATATTGATGGCCTGCCTATCGTCGAACAAACAGGT
CTACCTTTTGCTTCTCAACACCAAGGTCGCATGCATGCCTGTGGCCATGATTTTCATATGACCATTGCCTTAGGCTGTCT
TGAGCGCGCCCTTGAGGAGCAACCCAAGAATAATCTGCTCTTTCTATTTCAGCCTGCTGAAGAAAATGAAGCTGGTGGTA
TGCTCATGTATGAGGACGATGCTTTTGGAGATTGGTTGCCAGATCAATTTTATGGCCTCCATGTCCGTCCAGATTTGAAA
GTCGGCCAGATTGCGACTAATACTCATACACTCTTTGCAGGAACTTGCGAGGTGAAGATCCGTTTCAAAGGCAAAGGTGG
CCACGCAGCTTTTCCTCATGAAGCCAATGACGCCTTGGTGGCGGCTAGTTACTTTGTGACCCAAGTGCAGTCAGTTGTTA
GCCGCAATGTCAACCCAATCGAGGGAGCAGTGGTGACCTTCGGCGTTTTTCAAGCTGGAACCACCAACAATGTCATCACA
GACACAGCTTTTTTACATGGAACTATTCGTGCCTTGACACAGGACATGAGTCTCTTGGTGCAAAAGAGGGTAAAAACAGT
TGCAGAAGGGGTTGCAGCTGCCTTTGATATGGAAGTCGAAGTGGAACTCAAACAAGGTGGATACCTACCTGTTGAGAACA
ATCCAGCCTTGGCGCGTGAACTGATGGACTTCTTTGACGAAAAAGACGGAATCGAGTTGATTGATATCGAACCTGCTATG
ACAGGCGAGGACTTTGGTTATCTCCTTTCAAAGGTTGATGGCGTGATGTTTTGGCTAGGTATCGATAGTCCCTACGCTCT
TCATCACCCTCAAATGAGTCCTAAGGAAGAAGTCTTAGCTATTGGGGTGGCTGCGGTGTCTAGTTTCCTGAAAAAGAAGG
CAGCAGAATAG

Upstream 100 bases:

>100_bases
ACAGCGCTAGAGGATGCGCTTCGTACCTTGTAATTGTAAAAGTAAAAAAGAGGCGGAACCCTTTTTCCAGCCTCTTTCTG
CTATATCGGAGGACAGATAG

Downstream 100 bases:

>100_bases
AGGGCTTGTCTATGAAATCGGAATTACGCAAGCAAGTCTTGCATGAAATGAAGGCTATATCTCAAGAGCAAAAACAGGCT
ATAGACCAAGCTTTAACCGA

Product: peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTG
LPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFDMEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAM
TGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE

Sequences:

>Translated_376_residues
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTG
LPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFDMEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAM
TGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE
>Mature_376_residues
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTG
LPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFDMEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAM
TGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily

Homologues:

Organism=Escherichia coli, GI87081880, Length=414, Percent_Identity=24.6376811594203, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPL_STRP2 (Q04I78)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817310.1
- ProteinModelPortal:   Q04I78
- SMR:   Q04I78
- STRING:   Q04I78
- EnsemblBacteria:   EBSTRT00000020685
- GeneID:   4441204
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1922
- eggNOG:   COG1473
- GeneTree:   EBGT00050000027406
- HOGENOM:   HBG708500
- OMA:   RTWQTGI
- ProtClustDB:   CLSK876719
- HAMAP:   MF_01692
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.47

Molecular weight: Translated: 41680; Mature: 41680

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEECCCC
ERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLL
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLKVGQIATNTHTLFAGTCEVKI
EEEECCCCCCCCCEEEEECCCCCCCCCCHHEEEEECCCCCCCEEECCCEEEEEEEEEEEE
RFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEE
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFDMEVEVELKQGGYLPVENNPALARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHH
LMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLA
HHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCEECCCCCCCHHHHHH
IGVAAVSSFLKKKAAE
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEECCCC
ERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLL
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLKVGQIATNTHTLFAGTCEVKI
EEEECCCCCCCCCEEEEECCCCCCCCCCHHEEEEECCCCCCCEEECCCEEEEEEEEEEEE
RFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEE
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFDMEVEVELKQGGYLPVENNPALARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHH
LMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLA
HHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCEECCCCCCCHHHHHH
IGVAAVSSFLKKKAAE
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA