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The map label for this gene is ksgA [H]

Identifier: 221232709

GI number: 221232709

Start: 1946252

End: 1947124

Strand: Reverse

Name: ksgA [H]

Synonym: SPN23F_20050

Alternate gene names: 221232709

Gene position: 1947124-1946252 (Counterclockwise)

Preceding gene: 221232710

Following gene: 221232708

Centisome position: 87.66

GC content: 46.28

Gene sequence:

>873_bases
ATGAGAATTGCAGATTATAGCGTGACCAAGGCAGTGCTGGAGCGTCACGGTTTTACCTTTAAAAAGTCCTTTGGGCAAAA
TTTTTTGACGGATACCAATATCCTTCAAAAAATTGTGGATACGGCTGAAATTGATGATCAGGTCAATGTCATCGAAATCG
GGCCAGGTATTGGTGCCTTGACAGAATTTTTGGCTGAGCGTGCAGCCCAAGTCATGGCTTTTGAGATTGACCACCGTTTG
GTGCCAATTTTGGCAGATACCCTGCGTGATTTTGATAATGTGACCGTAGTTAACGAAGATATTCTCAAGGTTGATTTGGC
GCAACATATCCAGAATTTTAAAAATCCTGACCTGCCAATCAAGGTAGTGGCTAATTTGCCTTACTACATCACGACGCCTA
TTCTCATGCACTTGATTGAGAGTGGCATTCCTTTTTGTGAGTTTGTGGTCATGATGCAGAAAGAAGTAGCGGACCGCATT
TCAGCCCAGCCTAACACCAAGGCTTACGGTAGCTTGTCTATCGCCGTGCAGTATTACATGACAGCCAAGGTTGCCTTTAT
CGTGCCTCGTACGGTCTTTGTGCCAGCGCCAAATGTGGATTCAGCCATCTTGAAAATGGTGCGTCGTCCAGAGCCAGCCG
TAGCAGTAGAAGATGAGAACTTTTTCTTTAAGGTTTCCAAGGCTAGTTTTACCCATCGCCGCAAGACCTTGTGGAATAAC
TTGACAGGTTACTTTGGTAAGACTGAAGAGGTCAAGGACAAGCTGACCAAGGCTTTGGACCAGGCAGGCTTGTCACCAAG
TGTGCGTGGGGAAGCTCTCAGCTTGGCAGAATTTGCCGGTCTAGCAGACGCACTTAAAGGGCAAGGACTCTAA

Upstream 100 bases:

>100_bases
TTCTTCATGAGGAAAGATAGTTTTTTGGTATGATTTTCATTCCCAAAATACAAGGGGAATGTGTTACAATAGTAGTAACA
GATAATAGAAAAGAGAATAG

Downstream 100 bases:

>100_bases
GATGCAGGGACAAATCATTAAAGCCTTGGCAGGTTTCTACTATGTGGAGAGTGATGGCCAGGTTTATCAAACACGCGCGC
GTGGGAATTTCCGTAAAAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRL
VPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL

Sequences:

>Translated_290_residues
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRL
VPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL
>Mature_290_residues
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRL
VPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=282, Percent_Identity=27.6595744680851, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7657198, Length=225, Percent_Identity=30.6666666666667, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1786236, Length=270, Percent_Identity=32.2222222222222, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=27.5510204081633, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25146882, Length=230, Percent_Identity=31.304347826087, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=223, Percent_Identity=30.9417040358744, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358017, Length=228, Percent_Identity=30.2631578947368, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357273, Length=304, Percent_Identity=25, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32238; Mature: 32238

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGAL
CCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
TEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPDLPI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCCE
KVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEHHH
TAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHHHHHHHHH
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGAL
CCCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
TEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPDLPI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCCCCCE
KVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQPNTKAYGSLSIAVQYYM
EEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEHHH
TAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNN
HHHHHEEECCEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHHHHHHHHH
LTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA