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The map label for this gene is thiN [H]

Identifier: 221232706

GI number: 221232706

Start: 1944080

End: 1944742

Strand: Reverse

Name: thiN [H]

Synonym: SPN23F_20020

Alternate gene names: 221232706

Gene position: 1944742-1944080 (Counterclockwise)

Preceding gene: 221232707

Following gene: 221232705

Centisome position: 87.55

GC content: 44.65

Gene sequence:

>663_bases
ATGAGCGAGTACAAACTCTCAGAAAACAACTGGACTAGGGTTGCAGTTTTTGCAGGCGGAAACCGCGGTCATTATCGGAC
AGATTTTGATGCTTTTGTTGGGGTGGATCGAGGCTCGCTCTGGGTCTTGGAAGAAGACTTACCTCTTGCTCTAGCAGTCG
GAGATTTTGATTCTGTGACGGAAGAAGAGCGACAGGTGATTCAAAAAGGTGCCCAGTATTTTGTCCAAGCACGACCAGAA
AAGGATGATACAGATCTGGAATTGGCTCTCTTAACCATCTTTGAACAAAATCCTCAGGCTCAGGTCACTATTTTCGGTGC
CTTGGGTGGCCGTATTGACCATATGTTGGCCAATGTCTTTCTGCCTAGCAATCCTAAGTTGGCACCCTATATGCATCAAA
TAGAAATTGAGGATGGGCAAAACTTGATTACTTATTGTCCAGAAGGAATCAGTCAGCTAGAACCTCGTTCAGACTACGAC
TATCTAGCCTTTATGCCAGTTCGGGATAGCCAGCTGACTATTCTTGGAGCCAAGTATGAGTTGACAGAGGAAAATTTTTT
CTTTAAAAAAGTGTACGCTTCTAACGAATATATAGATAGGGAAGTGTCGGTAACTTGCCCAGATGGTTATGTGGTCGTAC
TGCATAGCAAGGACAGGAGGTAG

Upstream 100 bases:

>100_bases
GAAGTGGATGGTGGGATTGATGACCAAACTATTGCTCAAGCCAAAGAAGCCGGTGCGACTGTTTTTGTAGCAGGTTCCTA
TGTCTTTAAGGGAGAAGTCA

Downstream 100 bases:

>100_bases
GATGGAAAGTTTACTTATTCTATTATTAATTGCCAATCTAGCTGGTCTCTTTCTGATTTGGCAAAGGCAGGATAGGCAGG
AGAAACACTTAAGTAAGAGC

Product: thiamin pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPE
KDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR

Sequences:

>Translated_220_residues
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPE
KDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
>Mature_219_residues
SEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEK
DDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYDY
LAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 25074; Mature: 24943

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVT
CCCEEECCCCCEEEEEEECCCCCCEECCCHHEEECCCCEEEEEECCCCEEEEECCCHHCC
EEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVF
HHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHEE
LPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYDYLAFMPVRDSQLTILGAKYE
CCCCCCCCCEEEEEEECCCCEEEEECCCCHHHCCCCCCCCEEEEEEECCCEEEEEEEEEE
LTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
ECCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure 
SEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLEEDLPLALAVGDFDSVT
CCEEECCCCCEEEEEEECCCCCCEECCCHHEEECCCCEEEEEECCCCEEEEECCCHHCC
EEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQVTIFGALGGRIDHMLANVF
HHHHHHHHHHHHEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHEE
LPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYDYLAFMPVRDSQLTILGAKYE
CCCCCCCCCEEEEEEECCCCEEEEECCCCHHHCCCCCCCCEEEEEEECCCEEEEEEEEEE
LTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKDRR
ECCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]