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The map label for this gene is mtbP [H]

Identifier: 221232312

GI number: 221232312

Start: 1518330

End: 1519439

Strand: Reverse

Name: mtbP [H]

Synonym: SPN23F_15670

Alternate gene names: 221232312

Gene position: 1519439-1518330 (Counterclockwise)

Preceding gene: 221232313

Following gene: 221232311

Centisome position: 68.4

GC content: 41.53

Gene sequence:

>1110_bases
ATGGAATCAGCCGGGCATGAATGTATAGGATTTTGTGAAATAGACAAATTCGCTAGAGCTAGTTATAAAGCTATACACGA
TACGAAAGGAGAAATTGAATTACATGACATCACAACAGTATCAGATGACACTATTCGAGGAATCGGACATGTTGATGTTA
TCTGTGGAGGATTTCCGTGCCAGGCTTTCAGCATTGCAGGAAACAGACGAGGATTTGAAGATACAAGAGGAACTCTCTTC
TTTGAAATCGCAAGGTTCGCATCTATTCTCAAACCCAGATATCTATTCCTTGAAAACGTCAAGGGACTCCTCAACCACGA
CAGAGGAAATACCTTTGAGGTCATCCTCTCAGCGTTGGATGAACTGGGGTATGATGTGGAATGGCAAGTGCTTAACAGCA
AAAATTTTGGAGTCCCCCAAAATCGGGAACGTGTGTTCATTATCGGACATCTTAGAGGAGGAAGTGGACGAAAGGTATTT
CCTCTCAGCGGAGATGGTGCAGCGATTACTTGTGAACAACCAAAAATAAACAAGGTTGGAAATACTAGAAAAAAAGGGAA
ATCGCAAAGTGGTGATGTCGTCTCAATTGACTCATTAGCTCCCACCCTGTGTAGTACTACGACACAAAAAGATCCGTTAA
AAGTTTTAATTGAAAATGAAATAAAGCAGTTTGGTGTTCTGCAACCAAACTACAATCAAAGCGGCGTTGTTTATGAGATT
GATGGTATTTCACCAACAATCAGAGCGTATCAAGGAGGAAATCTTGAACCTAAAATCAGAGTAAAAGAAGCAACCAAGCA
AGGCTACCAAGAGGCTGAAATTGGAGATAGTGTAAACCTATCGCATCCAAACTCTAAAACACGCAGAGGGCGAGTAGGTA
AGCAGATAGCAAATACTCTCTTAACTGGAGAGAGTCAAGGTGTGGTTGAGCCTGATTTTAGGATTAGGAAGCTAACACCT
AGAGAATGCTGGAGGTTACAAGGTTTTCCTGATTGGGCTTTTGATAAGGCGCAGGAGGTCAACTCTAACAGTCAATTATA
CAAGCAAGCAGGCAATAGCGTAACAGTAAACGTTATTTCTGCAATAGCACAGGGGTTAGGAGGAAATTAA

Upstream 100 bases:

>100_bases
ATTAGTGATGATGATTTACCGTTTTAGCGAAAGGGGATATTCTAGTTAAGTTATGAAATTCTTAGACTTATTTGCTGGCA
TTGGAGGTTTTAGGATCGGC

Downstream 100 bases:

>100_bases
CCCTATTTAAACCAATTTGAAAAGGAAACAGAAAATGACAAAAATTGAAATCGTTATGGTACTTACAACTTTGATGTCTA
TCACATGGGCAGCGATTGTT

Product: DNA methylase

Products: NA

Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLF
FEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKNFGVPQNRERVFIIGHLRGGSGRKVF
PLSGDGAAITCEQPKINKVGNTRKKGKSQSGDVVSIDSLAPTLCSTTTQKDPLKVLIENEIKQFGVLQPNYNQSGVVYEI
DGISPTIRAYQGGNLEPKIRVKEATKQGYQEAEIGDSVNLSHPNSKTRRGRVGKQIANTLLTGESQGVVEPDFRIRKLTP
RECWRLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVISAIAQGLGGN

Sequences:

>Translated_369_residues
MESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLF
FEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKNFGVPQNRERVFIIGHLRGGSGRKVF
PLSGDGAAITCEQPKINKVGNTRKKGKSQSGDVVSIDSLAPTLCSTTTQKDPLKVLIENEIKQFGVLQPNYNQSGVVYEI
DGISPTIRAYQGGNLEPKIRVKEATKQGYQEAEIGDSVNLSHPNSKTRRGRVGKQIANTLLTGESQGVVEPDFRIRKLTP
RECWRLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVISAIAQGLGGN
>Mature_369_residues
MESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLF
FEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKNFGVPQNRERVFIIGHLRGGSGRKVF
PLSGDGAAITCEQPKINKVGNTRKKGKSQSGDVVSIDSLAPTLCSTTTQKDPLKVLIENEIKQFGVLQPNYNQSGVVYEI
DGISPTIRAYQGGNLEPKIRVKEATKQGYQEAEIGDSVNLSHPNSKTRRGRVGKQIANTLLTGESQGVVEPDFRIRKLTP
RECWRLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVISAIAQGLGGN

Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=396, Percent_Identity=28.2828282828283, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40575; Mature: 40575

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPC
CCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHCCCCCEEEEECCCCC
QAFSIAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALD
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ELGYDVEWQVLNSKNFGVPQNRERVFIIGHLRGGSGRKVFPLSGDGAAITCEQPKINKVG
HHCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCC
NTRKKGKSQSGDVVSIDSLAPTLCSTTTQKDPLKVLIENEIKQFGVLQPNYNQSGVVYEI
CCHHCCCCCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DGISPTIRAYQGGNLEPKIRVKEATKQGYQEAEIGDSVNLSHPNSKTRRGRVGKQIANTL
CCCCCEEEEECCCCCCCEEEEEHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LTGESQGVVEPDFRIRKLTPRECWRLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVIS
HCCCCCCCCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCEEEHHHHH
AIAQGLGGN
HHHHHCCCC
>Mature Secondary Structure
MESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPC
CCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHCCCCCEEEEECCCCC
QAFSIAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALD
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ELGYDVEWQVLNSKNFGVPQNRERVFIIGHLRGGSGRKVFPLSGDGAAITCEQPKINKVG
HHCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCEEEECCCCCCCCC
NTRKKGKSQSGDVVSIDSLAPTLCSTTTQKDPLKVLIENEIKQFGVLQPNYNQSGVVYEI
CCHHCCCCCCCCEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DGISPTIRAYQGGNLEPKIRVKEATKQGYQEAEIGDSVNLSHPNSKTRRGRVGKQIANTL
CCCCCEEEEECCCCCCCEEEEEHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LTGESQGVVEPDFRIRKLTPRECWRLQGFPDWAFDKAQEVNSNSQLYKQAGNSVTVNVIS
HCCCCCCCCCCCCEEEECCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHCCCEEEHHHHH
AIAQGLGGN
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]