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The map label for this gene is lpdA [H]

Identifier: 220932935

GI number: 220932935

Start: 2279798

End: 2281486

Strand: Direct

Name: lpdA [H]

Synonym: Hore_21030

Alternate gene names: 220932935

Gene position: 2279798-2281486 (Clockwise)

Preceding gene: 220932932

Following gene: 220932963

Centisome position: 88.43

GC content: 39.73

Gene sequence:

>1689_bases
ATGGTAGAATTAACATTGGATGGTTTATTAAATAATGCCAGTGAAGCTAAAGTTACCAGGGTTAATGTAGAAGAAGGTGA
AAAAATAAACAAAGGCGATCTCCTTTTTGAAGTAGAGGCCAATAAAGCCGCCATCCCTGTTGAATCTAACTATGAGGGAA
AAATTACTAAAATTGCTGTAAAAGAAGGAGATGTAGTTAAAAAGGGTGACCTTCTGGCTACCATAGAAGAAACAGAAGGT
GAGGCTAATCAAAAACAGGAAGATACCAATCCTGAAAAAGCAGGCAATGCTATGGAAGCTGACATTACTATTATCGGGGC
AGGCCCCGGTGGTTATGTAGCTGCCATTAAAGCAGCCCAGATGGGAGCTAAGGTAGTCCTGGTAGAAAAGGATAAACTGG
GAGGTACCTGTCTCAATCGTGGCTGTATACCTACTAAAGCCCTGGTCCGGTCTGCTGAAATATATAACTACCTGAAAGAA
GCAGATGATTTTGGCTGCCATGCCGAAAATATCTCCCTTAATATGAAAAAGGTTATTAAAAGAAAGGATAAAATTGTTTC
CCGCCTTGTCAAGGGTATTGAATTTCTCATGAGGAAAAATGGTGTTAAAGTCATCCAGGGAAGTGGTCAGATTAAAGACC
CCGAAACAGTCTATGTTAAAAAAGATAATGGTGATGTAGTCATTAACACCAGTAATATAATAATTGCTACCGGTTCAGTA
CCGGCCCATCTCCCCATTGAAGGAGCAGATTTACCGGGAGTTATTAACAGTGCTGAAGCCCTTGAGCTGGAAGAACTACC
TGAAAAAATGGTCATAGTCGGTGGTGGTGTCATTGGAATGGAATTTGCTTTTATCTTTAACAGTTTTGGAGTTGACGTTA
CTGTTGTCGAATACCTTGATGATATCCTGGCCAGCAATGATTCTGACATCTGTAGTGAAATTACATCAATAGCTGAAAAT
AGAAATATTAAAATATTCACCGGATCAAGGGTAGAAAAAATTAACGAAACTGAAGATGGTAAATATATTGTCTCGTTTAC
ACAGAATGATAAAAATAAATTTGTTACTGGAGATAAAGTCTTAATGGCTGTTGGTAGAAAACCCTTCTTTGAAGGAATCG
GAGTTGAAGAACTGGGACTGGAACTTAATGAAAAGGGTCGGGGTATAAAGGTTAATGATAAGATGCAAACCAGTATTCCC
AATATATATGCCATCGGTGATGTCACCAGTAAAATTTTACTGGCCCATGCTGCCTCCCACCAGGGTATAGTGGCTGTTAA
AAACATAATGGGAGAAGAATGCAAGATGGATTATGGGGCTATTCCCGGCGCTGTCTTTACCGACCCCGAGATTGCCACAG
TAGGGTATAATGAAAAAACAGCTACAGAAGCAGGCATTGAATATAATGTTGGGGTATTTCCCTTTAAAGCCAATGGTAAA
GTCCTAACCCTTGGTGAAAACAAAGGTTTTATAAAAATACTTACTGAAAAAGACACAGATAAGGTTATCGGTTGTTCCAT
GATAGGACCACATTCCACTGATTTGATTGCTGAAGTTACCCTGGCTGTTAAAAATGGCCTCAGAGCTGAAGACATAGCCG
AAACCATTCATGCTCATCCAACAACAGCAGAGGTAATCCATGAAGCGGCTCTGTCTGCTTATGAAAAACCACTCCATACT
TTTCAATAA

Upstream 100 bases:

>100_bases
AACTCTGATTATCAATATTATAAAAATGTCAACAAAATTACAATATTTAAATTAATATAATTTTAATGTGTAATACTAAA
CTAATAAGGAGGAACTAACA

Downstream 100 bases:

>100_bases
GTTGAAATGCAAACAATTTAAGTTTTTAATCCCCATTCAGAACTCCCACCCAGAGATGCTAATTATGCCAGGAAAACCAT
TAAAGAACGGGCTCCGTTAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MVELTLDGLLNNASEAKVTRVNVEEGEKINKGDLLFEVEANKAAIPVESNYEGKITKIAVKEGDVVKKGDLLATIEETEG
EANQKQEDTNPEKAGNAMEADITIIGAGPGGYVAAIKAAQMGAKVVLVEKDKLGGTCLNRGCIPTKALVRSAEIYNYLKE
ADDFGCHAENISLNMKKVIKRKDKIVSRLVKGIEFLMRKNGVKVIQGSGQIKDPETVYVKKDNGDVVINTSNIIIATGSV
PAHLPIEGADLPGVINSAEALELEELPEKMVIVGGGVIGMEFAFIFNSFGVDVTVVEYLDDILASNDSDICSEITSIAEN
RNIKIFTGSRVEKINETEDGKYIVSFTQNDKNKFVTGDKVLMAVGRKPFFEGIGVEELGLELNEKGRGIKVNDKMQTSIP
NIYAIGDVTSKILLAHAASHQGIVAVKNIMGEECKMDYGAIPGAVFTDPEIATVGYNEKTATEAGIEYNVGVFPFKANGK
VLTLGENKGFIKILTEKDTDKVIGCSMIGPHSTDLIAEVTLAVKNGLRAEDIAETIHAHPTTAEVIHEAALSAYEKPLHT
FQ

Sequences:

>Translated_562_residues
MVELTLDGLLNNASEAKVTRVNVEEGEKINKGDLLFEVEANKAAIPVESNYEGKITKIAVKEGDVVKKGDLLATIEETEG
EANQKQEDTNPEKAGNAMEADITIIGAGPGGYVAAIKAAQMGAKVVLVEKDKLGGTCLNRGCIPTKALVRSAEIYNYLKE
ADDFGCHAENISLNMKKVIKRKDKIVSRLVKGIEFLMRKNGVKVIQGSGQIKDPETVYVKKDNGDVVINTSNIIIATGSV
PAHLPIEGADLPGVINSAEALELEELPEKMVIVGGGVIGMEFAFIFNSFGVDVTVVEYLDDILASNDSDICSEITSIAEN
RNIKIFTGSRVEKINETEDGKYIVSFTQNDKNKFVTGDKVLMAVGRKPFFEGIGVEELGLELNEKGRGIKVNDKMQTSIP
NIYAIGDVTSKILLAHAASHQGIVAVKNIMGEECKMDYGAIPGAVFTDPEIATVGYNEKTATEAGIEYNVGVFPFKANGK
VLTLGENKGFIKILTEKDTDKVIGCSMIGPHSTDLIAEVTLAVKNGLRAEDIAETIHAHPTTAEVIHEAALSAYEKPLHT
FQ
>Mature_562_residues
MVELTLDGLLNNASEAKVTRVNVEEGEKINKGDLLFEVEANKAAIPVESNYEGKITKIAVKEGDVVKKGDLLATIEETEG
EANQKQEDTNPEKAGNAMEADITIIGAGPGGYVAAIKAAQMGAKVVLVEKDKLGGTCLNRGCIPTKALVRSAEIYNYLKE
ADDFGCHAENISLNMKKVIKRKDKIVSRLVKGIEFLMRKNGVKVIQGSGQIKDPETVYVKKDNGDVVINTSNIIIATGSV
PAHLPIEGADLPGVINSAEALELEELPEKMVIVGGGVIGMEFAFIFNSFGVDVTVVEYLDDILASNDSDICSEITSIAEN
RNIKIFTGSRVEKINETEDGKYIVSFTQNDKNKFVTGDKVLMAVGRKPFFEGIGVEELGLELNEKGRGIKVNDKMQTSIP
NIYAIGDVTSKILLAHAASHQGIVAVKNIMGEECKMDYGAIPGAVFTDPEIATVGYNEKTATEAGIEYNVGVFPFKANGK
VLTLGENKGFIKILTEKDTDKVIGCSMIGPHSTDLIAEVTLAVKNGLRAEDIAETIHAHPTTAEVIHEAALSAYEKPLHT
FQ

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=41.8803418803419, Blast_Score=356, Evalue=4e-98,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=31.5904139433551, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=29.2035398230088, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=29.2035398230088, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=29.2035398230088, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=29.2035398230088, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=29.2035398230088, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI291045266, Length=455, Percent_Identity=28.3516483516484, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=27.7533039647577, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=26.3858093126386, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=35.6043956043956, Blast_Score=298, Evalue=8e-82,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=27.8372591006424, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=29.7117516629712, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=29.3577981651376, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789065, Length=241, Percent_Identity=26.5560165975104, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=39.5299145299145, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI71983429, Length=434, Percent_Identity=27.4193548387097, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=434, Percent_Identity=27.4193548387097, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=25.6842105263158, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=468, Percent_Identity=25.8547008547009, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=37.5, Blast_Score=308, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=464, Percent_Identity=29.3103448275862, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=29.6137339055794, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=39.3162393162393, Blast_Score=339, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=26.8191268191268, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=26.4887063655031, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=26.7223382045929, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=27.0042194092827, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 60469; Mature: 60469

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELTLDGLLNNASEAKVTRVNVEEGEKINKGDLLFEVEANKAAIPVESNYEGKITKIAV
CEEEEECHHHCCCCCCEEEEEEHHCCCCCCCCCEEEEEECCCEEECCCCCCCCCEEEEEE
KEGDVVKKGDLLATIEETEGEANQKQEDTNPEKAGNAMEADITIIGAGPGGYVAAIKAAQ
ECCCEEECCCEEEEEECCCCCCCCCCCCCCHHHCCCCEEEEEEEEECCCCCEEEEEEHHH
MGAKVVLVEKDKLGGTCLNRGCIPTKALVRSAEIYNYLKEADDFGCHAENISLNMKKVIK
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
RKDKIVSRLVKGIEFLMRKNGVKVIQGSGQIKDPETVYVKKDNGDVVINTSNIIIATGSV
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCEEEECCEEEEEECCC
PAHLPIEGADLPGVINSAEALELEELPEKMVIVGGGVIGMEFAFIFNSFGVDVTVVEYLD
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHEEHHHHCCCCEEHHHHHH
DILASNDSDICSEITSIAENRNIKIFTGSRVEKINETEDGKYIVSFTQNDKNKFVTGDKV
HHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCEEEEEEECCCCCEECCCCE
LMAVGRKPFFEGIGVEELGLELNEKGRGIKVNDKMQTSIPNIYAIGDVTSKILLAHAASH
EEEECCCCHHCCCCHHHHCCEECCCCCCEEECCCHHHCCCCEEEECHHHHHHHHHHHCCC
QGIVAVKNIMGEECKMDYGAIPGAVFTDPEIATVGYNEKTATEAGIEYNVGVFPFKANGK
CCEEEEHHHCCHHHCCCCCCCCCCEECCCCEEEECCCCCCCHHCCCEECCEEEEEECCCE
VLTLGENKGFIKILTEKDTDKVIGCSMIGPHSTDLIAEVTLAVKNGLRAEDIAETIHAHP
EEEEECCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCC
TTAEVIHEAALSAYEKPLHTFQ
CHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MVELTLDGLLNNASEAKVTRVNVEEGEKINKGDLLFEVEANKAAIPVESNYEGKITKIAV
CEEEEECHHHCCCCCCEEEEEEHHCCCCCCCCCEEEEEECCCEEECCCCCCCCCEEEEEE
KEGDVVKKGDLLATIEETEGEANQKQEDTNPEKAGNAMEADITIIGAGPGGYVAAIKAAQ
ECCCEEECCCEEEEEECCCCCCCCCCCCCCHHHCCCCEEEEEEEEECCCCCEEEEEEHHH
MGAKVVLVEKDKLGGTCLNRGCIPTKALVRSAEIYNYLKEADDFGCHAENISLNMKKVIK
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
RKDKIVSRLVKGIEFLMRKNGVKVIQGSGQIKDPETVYVKKDNGDVVINTSNIIIATGSV
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCEEEECCEEEEEECCC
PAHLPIEGADLPGVINSAEALELEELPEKMVIVGGGVIGMEFAFIFNSFGVDVTVVEYLD
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHEEHHHHCCCCEEHHHHHH
DILASNDSDICSEITSIAENRNIKIFTGSRVEKINETEDGKYIVSFTQNDKNKFVTGDKV
HHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCCCEEEEEEECCCCCEECCCCE
LMAVGRKPFFEGIGVEELGLELNEKGRGIKVNDKMQTSIPNIYAIGDVTSKILLAHAASH
EEEECCCCHHCCCCHHHHCCEECCCCCCEEECCCHHHCCCCEEEECHHHHHHHHHHHCCC
QGIVAVKNIMGEECKMDYGAIPGAVFTDPEIATVGYNEKTATEAGIEYNVGVFPFKANGK
CCEEEEHHHCCHHHCCCCCCCCCCEECCCCEEEECCCCCCCHHCCCEECCEEEEEECCCE
VLTLGENKGFIKILTEKDTDKVIGCSMIGPHSTDLIAEVTLAVKNGLRAEDIAETIHAHP
EEEEECCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCC
TTAEVIHEAALSAYEKPLHTFQ
CHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]