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The map label for this gene is nfsA [C]

Identifier: 220931958

GI number: 220931958

Start: 1211898

End: 1212428

Strand: Direct

Name: nfsA [C]

Synonym: Hore_11180

Alternate gene names: 220931958

Gene position: 1211898-1212428 (Clockwise)

Preceding gene: 220931957

Following gene: 220931959

Centisome position: 47.01

GC content: 40.11

Gene sequence:

>531_bases
ATGGATATATATGAGGCAATTAAAAAGAGAAGGAGTATTCGTAAATACATATCAAAGCCTGTTGAAGAGGAAAAACTGAA
AAGGGTGCTGGAAGCAGGGCGGCTGGCTCCCTCAGGCAAAAATGTTCAGGCCTGGAAGTTTATTATTACCCGTGATGTTG
GGTTAAAAGATAAGCTCATAGAAGCCTGTAAAGGTCAAAAATTCATGAAAGAAGCTGATGCTATTATTACAGTATGTGTT
AATGAGGAGGAAGTTTATCAATATCATGGTAATTATATGACGTCCTTTGCTGTAGACGGAGCCATTGCCTTACAACAGAT
GATGTTGGCTGCTACTGCTGAAGGTCTTGGTACCTGCTGGATAGGAGCCTTTTATGAAAAAGATGTAAAAAAGATTCTTG
ATATACCAGCTCCATACCGGGTTATTGGATTAACTCCCCTGGGATACCCTGCTGAACGGGGAAGGGACAGGGGAAGAAAA
CCCTTAAAAGAAATTGTGTATAATGAAAAGTGGGGAAATAGTTACAATTAG

Upstream 100 bases:

>100_bases
ATGACATTATCAGGTGGTTTCAGGAGAATAATAGTTAATAGAAGACCATTAACAGTTCTTACATGTTACATATAATTAAT
ACATTAAAGGAGGTATTTAA

Downstream 100 bases:

>100_bases
ATTAACAATATAATAATAGTTATTTAAGGAGGTATGGCTATGGCGAGGATTAAAGTTGAAAGACCATCCCAGGAAAAACT
TAGAAAATTAGGAGTGGAAT

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDIYEAIKKRRSIRKYISKPVEEEKLKRVLEAGRLAPSGKNVQAWKFIITRDVGLKDKLIEACKGQKFMKEADAIITVCV
NEEEVYQYHGNYMTSFAVDGAIALQQMMLAATAEGLGTCWIGAFYEKDVKKILDIPAPYRVIGLTPLGYPAERGRDRGRK
PLKEIVYNEKWGNSYN

Sequences:

>Translated_176_residues
MDIYEAIKKRRSIRKYISKPVEEEKLKRVLEAGRLAPSGKNVQAWKFIITRDVGLKDKLIEACKGQKFMKEADAIITVCV
NEEEVYQYHGNYMTSFAVDGAIALQQMMLAATAEGLGTCWIGAFYEKDVKKILDIPAPYRVIGLTPLGYPAERGRDRGRK
PLKEIVYNEKWGNSYN
>Mature_176_residues
MDIYEAIKKRRSIRKYISKPVEEEKLKRVLEAGRLAPSGKNVQAWKFIITRDVGLKDKLIEACKGQKFMKEADAIITVCV
NEEEVYQYHGNYMTSFAVDGAIALQQMMLAATAEGLGTCWIGAFYEKDVKKILDIPAPYRVIGLTPLGYPAERGRDRGRK
PLKEIVYNEKWGNSYN

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20040; Mature: 20040

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIYEAIKKRRSIRKYISKPVEEEKLKRVLEAGRLAPSGKNVQAWKFIITRDVGLKDKLI
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHH
EACKGQKFMKEADAIITVCVNEEEVYQYHGNYMTSFAVDGAIALQQMMLAATAEGLGTCW
HHHCCHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGAFYEKDVKKILDIPAPYRVIGLTPLGYPAERGRDRGRKPLKEIVYNEKWGNSYN
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCHHCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDIYEAIKKRRSIRKYISKPVEEEKLKRVLEAGRLAPSGKNVQAWKFIITRDVGLKDKLI
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHH
EACKGQKFMKEADAIITVCVNEEEVYQYHGNYMTSFAVDGAIALQQMMLAATAEGLGTCW
HHHCCHHHHHHCCCEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGAFYEKDVKKILDIPAPYRVIGLTPLGYPAERGRDRGRKPLKEIVYNEKWGNSYN
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCHHCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]