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The map label for this gene is rfbA [H]

Identifier: 221230832

GI number: 221230832

Start: 2984167

End: 2985033

Strand: Reverse

Name: rfbA [H]

Synonym: MLBr_02503

Alternate gene names: 221230832

Gene position: 2985033-2984167 (Counterclockwise)

Preceding gene: 221230833

Following gene: 221230827

Centisome position: 91.34

GC content: 54.33

Gene sequence:

>867_bases
ATGCGGGGGATTATCTTGGCTGGCGGCTCTGGTACACGGCTGTACCCAATCACGCTGGGCATTAGCAAGCAGTTATTGCC
GGTATACGACAAACCTATGATTTATTATCCGCTGTCCACGCTGATGATGGCCGGCATCCGCGACATCTTGGTGATTACCA
CCGCTCACGACGCACCCGGGTTTAAGCGACTCTTAGGAGACGGCACACAATTTGGTGTCAACATCAGCTACGCGACACAG
GATCATCCCGACGGTCTGGCGCAGGCATTCGTCATTGGTGCCAATCACATCGGCGCCGACACAGTGGCATTGGTTCTGGG
GGACAACATTTTCTATGGACCCGGACTGGGGACAAGCCTGAGACGCTTCCAATATGTCAGCGGAGGAGCTATTTTCGCCT
ATTGTGTGGCTAATCCGTCGTCCTATGGTATCGTAGAGCTGGGTATTGATGGCATAGCGTTATCCTTAGAGGAGAAACCC
GCCACGCCAAAATCTCAGTACGCGGTTCCGGGTTTGTATTTCTATGACAACGACGTGGTCGAGATTGCCAGGGGCTTAAC
TAAATCAGCGCGAGGTGAATATGAGATCACGGAGGTTAATCAGATCTACCTCAACCAAGGCCGGCTGACGGTCGAAGTGT
TGGCTCGCGGGACGGCATGGCTTGACACGGGGACTTTCGACTCACTGCTGGACGCTAGTGACTTTGTCCGCACGTTGGAA
CGTCGGCAAGGTTTGAAGGTCAGCGTTCCCGAGGAAGTGTCGTGGCGGATGGGTTGGATCGACGACGAGCAGCTCGCCTT
GCGCGCACATAGCTTGGCTAAGTCAGGGTATGGCTGTTACCTGTCAGAACTTTTGGAACGCGGTTGA

Upstream 100 bases:

>100_bases
GGTGTTCCGGTATGAGACTTTGGTCGGCGCTAGAAGCCGTCGATCTCACTGAAGCTGATTTCGTCAATCAGTGGTCGATA
AGAAGCGTTAGGAGCTACCT

Downstream 100 bases:

>100_bases
TTTCGGCAGGCTTAGCCAACACCGCGGCGACTGTTGTGGTCAACGGGACTGACGGAGCAAGCGCTATGCCATCCACCGCC
GAGCGCACCAACTCGATGAC

Product: glucose-1-phosphate thymidyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRGIILAGGSGTRLYPITLGISKQLLPVYDKPMIYYPLSTLMMAGIRDILVITTAHDAPGFKRLLGDGTQFGVNISYATQ
DHPDGLAQAFVIGANHIGADTVALVLGDNIFYGPGLGTSLRRFQYVSGGAIFAYCVANPSSYGIVELGIDGIALSLEEKP
ATPKSQYAVPGLYFYDNDVVEIARGLTKSARGEYEITEVNQIYLNQGRLTVEVLARGTAWLDTGTFDSLLDASDFVRTLE
RRQGLKVSVPEEVSWRMGWIDDEQLALRAHSLAKSGYGCYLSELLERG

Sequences:

>Translated_288_residues
MRGIILAGGSGTRLYPITLGISKQLLPVYDKPMIYYPLSTLMMAGIRDILVITTAHDAPGFKRLLGDGTQFGVNISYATQ
DHPDGLAQAFVIGANHIGADTVALVLGDNIFYGPGLGTSLRRFQYVSGGAIFAYCVANPSSYGIVELGIDGIALSLEEKP
ATPKSQYAVPGLYFYDNDVVEIARGLTKSARGEYEITEVNQIYLNQGRLTVEVLARGTAWLDTGTFDSLLDASDFVRTLE
RRQGLKVSVPEEVSWRMGWIDDEQLALRAHSLAKSGYGCYLSELLERG
>Mature_288_residues
MRGIILAGGSGTRLYPITLGISKQLLPVYDKPMIYYPLSTLMMAGIRDILVITTAHDAPGFKRLLGDGTQFGVNISYATQ
DHPDGLAQAFVIGANHIGADTVALVLGDNIFYGPGLGTSLRRFQYVSGGAIFAYCVANPSSYGIVELGIDGIALSLEEKP
ATPKSQYAVPGLYFYDNDVVEIARGLTKSARGEYEITEVNQIYLNQGRLTVEVLARGTAWLDTGTFDSLLDASDFVRTLE
RRQGLKVSVPEEVSWRMGWIDDEQLALRAHSLAKSGYGCYLSELLERG

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide,

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=284, Percent_Identity=61.6197183098592, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1790224, Length=286, Percent_Identity=60.1398601398601, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1787488, Length=236, Percent_Identity=26.6949152542373, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=255, Percent_Identity=25.0980392156863, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355443, Length=251, Percent_Identity=24.3027888446215, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644084, Length=251, Percent_Identity=24.3027888446215, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31326; Mature: 31326

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGIILAGGSGTRLYPITLGISKQLLPVYDKPMIYYPLSTLMMAGIRDILVITTAHDAPG
CCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEEECCCCCC
FKRLLGDGTQFGVNISYATQDHPDGLAQAFVIGANHIGADTVALVLGDNIFYGPGLGTSL
HHHHHCCCCEEEEEEEEECCCCCCHHHEEEEEECCCCCCCEEEEEECCCEEECCCCCCHH
RRFQYVSGGAIFAYCVANPSSYGIVELGIDGIALSLEEKPATPKSQYAVPGLYFYDNDVV
HHEEEECCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCEEEEECCCHH
EIARGLTKSARGEYEITEVNQIYLNQGRLTVEVLARGTAWLDTGTFDSLLDASDFVRTLE
HHHHHHHHHCCCCEEEEEEEEEEEECCCEEEEEEECCCEEEECCCHHHHHCHHHHHHHHH
RRQGLKVSVPEEVSWRMGWIDDEQLALRAHSLAKSGYGCYLSELLERG
HHCCCEEECCHHCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRGIILAGGSGTRLYPITLGISKQLLPVYDKPMIYYPLSTLMMAGIRDILVITTAHDAPG
CCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEEECCCCCC
FKRLLGDGTQFGVNISYATQDHPDGLAQAFVIGANHIGADTVALVLGDNIFYGPGLGTSL
HHHHHCCCCEEEEEEEEECCCCCCHHHEEEEEECCCCCCCEEEEEECCCEEECCCCCCHH
RRFQYVSGGAIFAYCVANPSSYGIVELGIDGIALSLEEKPATPKSQYAVPGLYFYDNDVV
HHEEEECCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCEEEEECCCHH
EIARGLTKSARGEYEITEVNQIYLNQGRLTVEVLARGTAWLDTGTFDSLLDASDFVRTLE
HHHHHHHHHCCCCEEEEEEEEEEEECCCEEEEEEECCCEEEECCCHHHHHCHHHHHHHHH
RRQGLKVSVPEEVSWRMGWIDDEQLALRAHSLAKSGYGCYLSELLERG
HHCCCEEECCHHCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084178; 9634230; 12218036 [H]