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The map label for this gene is pyrE

Identifier: 221230820

GI number: 221230820

Start: 2960867

End: 2961406

Strand: Reverse

Name: pyrE

Synonym: MLBr_02487

Alternate gene names: 221230820

Gene position: 2961406-2960867 (Counterclockwise)

Preceding gene: 221230821

Following gene: 221230819

Centisome position: 90.62

GC content: 62.59

Gene sequence:

>540_bases
GTGGCCGAACTTGACCGAAAGGAGTTGGCTGATCTGGTGCGCCGGCTGTCGGTAGTGCAAGGGTGCGTCACGCTGTCGTC
GGGCAAACAGGCCGACCACTATGTCGATTTGCGCCGCGCCACCTTGCACCACCGGGCCTCAGCGCTGATCGGCCGGCTGA
TGCGCGAACTTACAAAAGACTGGAACTACACGGTGGTCGGCGGCTTGACCTTGGGTGCCGACCCTGTAGCGATAGCTATC
ATACACTCGCCTGGGCGTCCGATCGACGCGTTCGTGGTACGCAAGTACGCGAAAACCCATGGTTTACAACGTCTTATCGA
GGGATCTGAGGTATCTGGTCAGCGTGTGTTGGTGGTGGAGGACACGAGTACGACCGGGGCTTCGGCACTGACAGCGGTGC
GCGCGGTACAAGACGCGGGCGGCGACGTGGTTGGCGTGGCCACCGTGGTGGACCGCGGTACCGGCGCGGCCGAGGCCATC
AAAGCTGAAGGACTGCTCTACCGTAGCGTGCTGGGCCTTGCCGATTTGGGACTGGGCTAG

Upstream 100 bases:

>100_bases
TATTCAGCATTCTGGTTCTGCAGTGCCAGGCGATTGTCACTGTGGGGAGAATGATCCGTGGAGTCTGTTGCGTGCCGTAA
ACAAACGAGTGGGGGCGGCT

Downstream 100 bases:

>100_bases
CCTCTACCGCGATCATGGTGCTCGCCTTTCCTTGTGATTATCGCCATGGTATTGTTCGGGGCTCGAGCCATGTTGGTCGT
CCGTATGGTGCTCATGGCCA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MAELDRKELADLVRRLSVVQGCVTLSSGKQADHYVDLRRATLHHRASALIGRLMRELTKDWNYTVVGGLTLGADPVAIAI
IHSPGRPIDAFVVRKYAKTHGLQRLIEGSEVSGQRVLVVEDTSTTGASALTAVRAVQDAGGDVVGVATVVDRGTGAAEAI
KAEGLLYRSVLGLADLGLG

Sequences:

>Translated_179_residues
MAELDRKELADLVRRLSVVQGCVTLSSGKQADHYVDLRRATLHHRASALIGRLMRELTKDWNYTVVGGLTLGADPVAIAI
IHSPGRPIDAFVVRKYAKTHGLQRLIEGSEVSGQRVLVVEDTSTTGASALTAVRAVQDAGGDVVGVATVVDRGTGAAEAI
KAEGLLYRSVLGLADLGLG
>Mature_178_residues
AELDRKELADLVRRLSVVQGCVTLSSGKQADHYVDLRRATLHHRASALIGRLMRELTKDWNYTVVGGLTLGADPVAIAII
HSPGRPIDAFVVRKYAKTHGLQRLIEGSEVSGQRVLVVEDTSTTGASALTAVRAVQDAGGDVVGVATVVDRGTGAAEAIK
AEGLLYRSVLGLADLGLG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=177, Percent_Identity=28.8135593220339, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_MYCLE (Q9CB28)

Other databases:

- EMBL:   AL583925
- PIR:   D87220
- RefSeq:   NP_302606.1
- ProteinModelPortal:   Q9CB28
- SMR:   Q9CB28
- EnsemblBacteria:   EBMYCT00000028749
- GeneID:   908475
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2487
- NMPDR:   fig|272631.1.peg.1478
- Leproma:   ML2487
- GeneTree:   EBGT00050000017679
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- BioCyc:   MLEP272631:ML2487-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 18879; Mature: 18748

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 94-94 BINDING 95-95 BINDING 98-98 BINDING 100-100 BINDING 124-124 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELDRKELADLVRRLSVVQGCVTLSSGKQADHYVDLRRATLHHRASALIGRLMRELTKD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WNYTVVGGLTLGADPVAIAIIHSPGRPIDAFVVRKYAKTHGLQRLIEGSEVSGQRVLVVE
CCEEEEECEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
DTSTTGASALTAVRAVQDAGGDVVGVATVVDRGTGAAEAIKAEGLLYRSVLGLADLGLG
CCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AELDRKELADLVRRLSVVQGCVTLSSGKQADHYVDLRRATLHHRASALIGRLMRELTKD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
WNYTVVGGLTLGADPVAIAIIHSPGRPIDAFVVRKYAKTHGLQRLIEGSEVSGQRVLVVE
CCEEEEECEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
DTSTTGASALTAVRAVQDAGGDVVGVATVVDRGTGAAEAIKAEGLLYRSVLGLADLGLG
CCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002