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The map label for this gene is murB

Identifier: 221230800

GI number: 221230800

Start: 2922687

End: 2923790

Strand: Reverse

Name: murB

Synonym: MLBr_02447

Alternate gene names: 221230800

Gene position: 2923790-2922687 (Counterclockwise)

Preceding gene: 221230803

Following gene: 221230799

Centisome position: 89.47

GC content: 62.68

Gene sequence:

>1104_bases
ATGAAACGGAGCGCTGTCGGTTCGTTCTTTGCCGGCGCGCATGTCGCAGAGGCGGTGTCACTTGCGCCGTTGACCACGTT
GCGGGTCGGTCCGGTCGCCCAGCGGGTTATAACGTGCGCCAGCACTGAACAGGTAGTCGACGTTATACGGCAGCTAGACG
CACCAGCCGGCGGCAGGGGAGCTGGGCCGCTGCTGATATTTGCTGGCGGCTCCAACCTGGTGATTGCCGACACGCTGGCC
GACTTGACTGCGGTGCGCTTGGCCAATGCCGGCATCACTATCGACGGCAACCTAGTGCGTGCCGAGGCTGGCGCGGTCTG
GGACGACGTGGTGGTTAGGGCCATCGAGCACGGGCTCGGTGGGCTGGAATGCCTGTCTGGTATCCCCGGATCGGCTGGGG
CCACTCCGGTGCAAAATGTGGGTGCCTACGGCGCAGAAGTGTCTGACACAATCACTCGGGTCCGGCTGCTGGAGCGCAGT
AGCGGCTCGGTTCGTTGGGTCTCCGCCTGCGAGTTGCGTTTTGGTTATCGTACCAGCGTTTTCAAGCAAGCCGACCCGTC
AGGGTCGCAACCGCCTGCTGTGGTGCTGGAAGTCGAGTTCAAGCTCGATGCATCGGGGCAGAGCGCACCGTTGCACTATG
GTGAGCTGGTGGCCACACTCGACGCGACTAGTGGGCAGCGCGCCAACCCACACGCGGTGCGCGAGGCGGTGCTGGCGCTG
CGTGTTCGCAAGGGTATGGTGCTCGATGCGGCAGACCATGACACCTGGAGTGTCGGATCTTTCTTTACTAATCCGGTAGT
AGCGCCGGACGTTTACCGTCAGCTAGCCAAGATGGTGGACGGGCCGGTGCCGCATTATCCGGCGCAGGACGGCGTCAAGC
TGGCCGCTGGCTGGCTGGTGGAACGGGCCGGCTTCGGTAAGGGTTATCCTGATGGTGCAGCTCCATGCCGGCTTTCCACC
AAACATGTGCTTGCCTTAACCAACCGCGGTGCCGCCACCGCCGAAGATGTGGTAACTCTGGCCCGCACCGTTCGTAATGG
GGTCCTAGAGGTGTTTGGTATCACACTCGAACCTGAGCCTGCACTGGTTGGGTGCGTGTTGTAG

Upstream 100 bases:

>100_bases
GCCTCGTTTAATGTCTGAAAAATGTTTTCGACACCGCCTTCGTAGTCGGCGGACATGTCGCATTATAGCGGCATAGTAGG
CCAGTCTATCGTTACCTGCC

Downstream 100 bases:

>100_bases
CTATTGGATACGGGTTACCGTATTTTGCGGAAAGTGTTTAGGGAGGGGGAAAGTCGCCTGGCCCGGTATCTTTGTGTATC
GTGATCGCATCCACTCCGCG

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKRSAVGSFFAGAHVAEAVSLAPLTTLRVGPVAQRVITCASTEQVVDVIRQLDAPAGGRGAGPLLIFAGGSNLVIADTLA
DLTAVRLANAGITIDGNLVRAEAGAVWDDVVVRAIEHGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLERS
SGSVRWVSACELRFGYRTSVFKQADPSGSQPPAVVLEVEFKLDASGQSAPLHYGELVATLDATSGQRANPHAVREAVLAL
RVRKGMVLDAADHDTWSVGSFFTNPVVAPDVYRQLAKMVDGPVPHYPAQDGVKLAAGWLVERAGFGKGYPDGAAPCRLST
KHVLALTNRGAATAEDVVTLARTVRNGVLEVFGITLEPEPALVGCVL

Sequences:

>Translated_367_residues
MKRSAVGSFFAGAHVAEAVSLAPLTTLRVGPVAQRVITCASTEQVVDVIRQLDAPAGGRGAGPLLIFAGGSNLVIADTLA
DLTAVRLANAGITIDGNLVRAEAGAVWDDVVVRAIEHGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLERS
SGSVRWVSACELRFGYRTSVFKQADPSGSQPPAVVLEVEFKLDASGQSAPLHYGELVATLDATSGQRANPHAVREAVLAL
RVRKGMVLDAADHDTWSVGSFFTNPVVAPDVYRQLAKMVDGPVPHYPAQDGVKLAAGWLVERAGFGKGYPDGAAPCRLST
KHVLALTNRGAATAEDVVTLARTVRNGVLEVFGITLEPEPALVGCVL
>Mature_367_residues
MKRSAVGSFFAGAHVAEAVSLAPLTTLRVGPVAQRVITCASTEQVVDVIRQLDAPAGGRGAGPLLIFAGGSNLVIADTLA
DLTAVRLANAGITIDGNLVRAEAGAVWDDVVVRAIEHGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLERS
SGSVRWVSACELRFGYRTSVFKQADPSGSQPPAVVLEVEFKLDASGQSAPLHYGELVATLDATSGQRANPHAVREAVLAL
RVRKGMVLDAADHDTWSVGSFFTNPVVAPDVYRQLAKMVDGPVPHYPAQDGVKLAAGWLVERAGFGKGYPDGAAPCRLST
KHVLALTNRGAATAEDVVTLARTVRNGVLEVFGITLEPEPALVGCVL

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=352, Percent_Identity=35.5113636363636, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_MYCLB (B8ZT90)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504216.1
- ProteinModelPortal:   B8ZT90
- EnsemblBacteria:   EBMYCT00000085902
- GeneID:   7324761
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015845
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   PRK13903
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 38113; Mature: 38113

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 177-177 ACT_SITE 260-260 ACT_SITE 359-359

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSAVGSFFAGAHVAEAVSLAPLTTLRVGPVAQRVITCASTEQVVDVIRQLDAPAGGRG
CCCCHHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
AGPLLIFAGGSNLVIADTLADLTAVRLANAGITIDGNLVRAEAGAVWDDVVVRAIEHGLG
CCCEEEEECCCCEEEEHHHHHHHHHHHHCCCEEECCCEEEECCCCHHHHHHHHHHHHCCC
GLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLERSSGSVRWVSACELRFGYRTSV
HHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHH
FKQADPSGSQPPAVVLEVEFKLDASGQSAPLHYGELVATLDATSGQRANPHAVREAVLAL
HHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHH
RVRKGMVLDAADHDTWSVGSFFTNPVVAPDVYRQLAKMVDGPVPHYPAQDGVKLAAGWLV
HHHCCCEEECCCCCCEECCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
ERAGFGKGYPDGAAPCRLSTKHVLALTNRGAATAEDVVTLARTVRNGVLEVFGITLEPEP
HHCCCCCCCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCC
ALVGCVL
CEEEECC
>Mature Secondary Structure
MKRSAVGSFFAGAHVAEAVSLAPLTTLRVGPVAQRVITCASTEQVVDVIRQLDAPAGGRG
CCCCHHHHHHHHHHHHHHHHHCCHHHEECCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
AGPLLIFAGGSNLVIADTLADLTAVRLANAGITIDGNLVRAEAGAVWDDVVVRAIEHGLG
CCCEEEEECCCCEEEEHHHHHHHHHHHHCCCEEECCCEEEECCCCHHHHHHHHHHHHCCC
GLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLERSSGSVRWVSACELRFGYRTSV
HHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHH
FKQADPSGSQPPAVVLEVEFKLDASGQSAPLHYGELVATLDATSGQRANPHAVREAVLAL
HHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHH
RVRKGMVLDAADHDTWSVGSFFTNPVVAPDVYRQLAKMVDGPVPHYPAQDGVKLAAGWLV
HHHCCCEEECCCCCCEECCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
ERAGFGKGYPDGAAPCRLSTKHVLALTNRGAATAEDVVTLARTVRNGVLEVFGITLEPEP
HHCCCCCCCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCC
ALVGCVL
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA