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The map label for this gene is galE [C]

Identifier: 221230786

GI number: 221230786

Start: 2903478

End: 2904572

Strand: Reverse

Name: galE [C]

Synonym: MLBr_02428

Alternate gene names: 221230786

Gene position: 2904572-2903478 (Counterclockwise)

Preceding gene: 221230787

Following gene: 221230785

Centisome position: 88.88

GC content: 61.1

Gene sequence:

>1095_bases
GTGGATTCCTCGAGCGGTCAGGATGGCGGTGGAACAGGCGGAGTCGTGCCCTACCCCAAGGTAGTACTGGTTACTGGTGC
TTGCCGCTTTCTCGGCGGCTATCTGACTGCTCGGTTGGCGCAAAACCCTTTGATCAATGCGGTCATCGCGGTGGATGCGA
TTGCGCCCAGCAAGGACATGCTGCGTCGGATGGGGCACGCCGAGTTCGTGCGCGCCGATATTCGTAATCCCTTTATCGCC
AAGGTGATTCGCAACGGCGAGGTCGATACGGTCGTGCACGCGGACGCAGCGTCCTATGCGCCGCGCTCCTGCGGTAGTGC
GGCGTTGAAGGAACTCAATGTGATGGGCGCGATGCAACTATTCGCCGCCTGCCAGAAGGCGCCCACCGTGCGTCGTGTCG
TGCTGAAGTCGACCTCGGCCGTTTACGGATCGGGTTCCCGCGATCCGGTGATGTTCACCGAGGACAGCAGTAGTTGGCGT
TCTTTCCGTGAGGGTTTCGCCAAGGACAGCCTCGATATCGAGGGTTACGCACGCGGGCTGGGCCGGCGCCGGTCCGATAT
CGCGGTAACCATTCTGCGGTTGGCTGACATGATCGGCCCGGCGATGGACACCACGTTGTCGCGGTATCTGGCCGGGCCGT
TGGTGCCGACGATGTGCGGCCGTGATGCACGGCTGCAGCTGCTTCACGAACAGGATGCGCTGGGCGCACTGGAGTGCGCG
GCGATGACCGGCAAGGCTGGCACCTTCAACATCGGCGCCAGTGGCATTATGATGCTTTCGCAAGCTATTCGGCGGGCTGG
CCGGATCGCGGTGCCGATACCCAGCTTCGGCGTCTGGGCACTTGATTTGCTAAGGTGGGTGAATCGCTACACCGAAATCA
CTCGAGATCAGTTTGAGTACCTGAGCTATGGTCGGGTCATGGACACCACCAGGATGGGTTCCGAACTCGGTTATCACCCG
AAGTGGACGACGGCCGGGGCGTTCGATGACTACGTTCGCGGCCGAGGTTTGACTCCCATAATTGACCTACATCGGGTACG
CTACTTCGAGGGTCGCGCCATAGCGTTAGCGCAGCGCTGGAGTAGCCGAAATTGA

Upstream 100 bases:

>100_bases
CGGGTGCAGCGCAGAAAACTTGGCAAGTAGCCGCCGCTACATGTTCCGATTTTCATACGGTTGTAGAGCCACTGTTTCTT
CTGGCCTTTAGGCTCACCAA

Downstream 100 bases:

>100_bases
ATTCCGTGGGGTGGGGTCAGGTAGATACTGTGGTGGGCGAAACCAGAGCGAATGTTATTCCGTTGCATATCAAGAGGGGT
CGGGTAGCATCACGTCGGAG

Product: putative glucose epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MDSSSGQDGGGTGGVVPYPKVVLVTGACRFLGGYLTARLAQNPLINAVIAVDAIAPSKDMLRRMGHAEFVRADIRNPFIA
KVIRNGEVDTVVHADAASYAPRSCGSAALKELNVMGAMQLFAACQKAPTVRRVVLKSTSAVYGSGSRDPVMFTEDSSSWR
SFREGFAKDSLDIEGYARGLGRRRSDIAVTILRLADMIGPAMDTTLSRYLAGPLVPTMCGRDARLQLLHEQDALGALECA
AMTGKAGTFNIGASGIMMLSQAIRRAGRIAVPIPSFGVWALDLLRWVNRYTEITRDQFEYLSYGRVMDTTRMGSELGYHP
KWTTAGAFDDYVRGRGLTPIIDLHRVRYFEGRAIALAQRWSSRN

Sequences:

>Translated_364_residues
MDSSSGQDGGGTGGVVPYPKVVLVTGACRFLGGYLTARLAQNPLINAVIAVDAIAPSKDMLRRMGHAEFVRADIRNPFIA
KVIRNGEVDTVVHADAASYAPRSCGSAALKELNVMGAMQLFAACQKAPTVRRVVLKSTSAVYGSGSRDPVMFTEDSSSWR
SFREGFAKDSLDIEGYARGLGRRRSDIAVTILRLADMIGPAMDTTLSRYLAGPLVPTMCGRDARLQLLHEQDALGALECA
AMTGKAGTFNIGASGIMMLSQAIRRAGRIAVPIPSFGVWALDLLRWVNRYTEITRDQFEYLSYGRVMDTTRMGSELGYHP
KWTTAGAFDDYVRGRGLTPIIDLHRVRYFEGRAIALAQRWSSRN
>Mature_364_residues
MDSSSGQDGGGTGGVVPYPKVVLVTGACRFLGGYLTARLAQNPLINAVIAVDAIAPSKDMLRRMGHAEFVRADIRNPFIA
KVIRNGEVDTVVHADAASYAPRSCGSAALKELNVMGAMQLFAACQKAPTVRRVVLKSTSAVYGSGSRDPVMFTEDSSSWR
SFREGFAKDSLDIEGYARGLGRRRSDIAVTILRLADMIGPAMDTTLSRYLAGPLVPTMCGRDARLQLLHEQDALGALECA
AMTGKAGTFNIGASGIMMLSQAIRRAGRIAVPIPSFGVWALDLLRWVNRYTEITRDQFEYLSYGRVMDTTRMGSELGYHP
KWTTAGAFDDYVRGRGLTPIIDLHRVRYFEGRAIALAQRWSSRN

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 39637; Mature: 39637

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSSGQDGGGTGGVVPYPKVVLVTGACRFLGGYLTARLAQNPLINAVIAVDAIAPSKDM
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
LRRMGHAEFVRADIRNPFIAKVIRNGEVDTVVHADAASYAPRSCGSAALKELNVMGAMQL
HHHCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHH
FAACQKAPTVRRVVLKSTSAVYGSGSRDPVMFTEDSSSWRSFREGFAKDSLDIEGYARGL
HHHHHHCCHHHHHHHHHCCHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHH
GRRRSDIAVTILRLADMIGPAMDTTLSRYLAGPLVPTMCGRDARLQLLHEQDALGALECA
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCHHHHHHH
AMTGKAGTFNIGASGIMMLSQAIRRAGRIAVPIPSFGVWALDLLRWVNRYTEITRDQFEY
HHCCCCCEEECCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSYGRVMDTTRMGSELGYHPKWTTAGAFDDYVRGRGLTPIIDLHRVRYFEGRAIALAQRW
HHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEHHHC
SSRN
CCCC
>Mature Secondary Structure
MDSSSGQDGGGTGGVVPYPKVVLVTGACRFLGGYLTARLAQNPLINAVIAVDAIAPSKDM
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHH
LRRMGHAEFVRADIRNPFIAKVIRNGEVDTVVHADAASYAPRSCGSAALKELNVMGAMQL
HHHCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCHHHCCCCCHHHHHHHHHHHHHHHHH
FAACQKAPTVRRVVLKSTSAVYGSGSRDPVMFTEDSSSWRSFREGFAKDSLDIEGYARGL
HHHHHHCCHHHHHHHHHCCHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHH
GRRRSDIAVTILRLADMIGPAMDTTLSRYLAGPLVPTMCGRDARLQLLHEQDALGALECA
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCHHHHHHH
AMTGKAGTFNIGASGIMMLSQAIRRAGRIAVPIPSFGVWALDLLRWVNRYTEITRDQFEY
HHCCCCCEEECCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSYGRVMDTTRMGSELGYHPKWTTAGAFDDYVRGRGLTPIIDLHRVRYFEGRAIALAQRW
HHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCEEHHHC
SSRN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]