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The map label for this gene is hemL

Identifier: 221230777

GI number: 221230777

Start: 2884599

End: 2885939

Strand: Reverse

Name: hemL

Synonym: MLBr_02414

Alternate gene names: 221230777

Gene position: 2885939-2884599 (Counterclockwise)

Preceding gene: 221230778

Following gene: 221230776

Centisome position: 88.31

GC content: 65.4

Gene sequence:

>1341_bases
GTGGGCAGCACTGACCAGGCAACCGCACCGGCCGGCCCAGCCGTAAGCATTTCGGCGAAGCTGTTTGAGGATGCCTGCGC
CGTCATTCCCGGTGGGGTGAACTCCCCGGTACGCGCCTTCTCGGCGGTCGGTGGCACCCCGCTCTTCATCACCGAAGCGC
GCGGTTGCTGGCTAACCGACGCCGACGGCCGCCGCTATGTCGATCTGGTCTGCTCGTGGGGGCCGATGATCCTCGGTCAT
GCGCATCCCGCCGTGGTTGACGCTGTCGCTACCGTTGCTGCCTCCGGGCTGTCGTTCGGGGCACCGACTCCGGCGGAGAC
GCAGCTGGCCGCCGAGATCATCGGGCGGATGGCGCCCGTCGAGCGAATACGGCTGGTGAACTCTGGCACTGAAGCCACCA
TGAGCGCGGTCCGGTTGGCCCGTGGATTCACCGGACGGACCAAGATCATAAAGTTCTCTGGCTGCTATCACGGCCATGTG
GACGCGCTATTAGCCGACGCGGGCTCTGGGGTGGCGACGCTGAGCCTACCGTCCTCGCCGGGAGTGACTGGAGCCGCCGC
AGCTGACACAATTGTGTTGCCCTACAATGACATCGAGGCCGTTCGGCAGACGTTTGCCCGGTTGGGGGACCAGATCGCCG
CGGTGATTACCGAAGCGAGTCCCGGCAATATGGGAGTTGTCCCACCCGCGCCCGGCTACAACGCGGCGCTGCGCGCGATC
ACCGCAGAGCATGGTGCGCTACTGATCATCGACGAGGTGATGACCGGTTTCCGGGTCAGCCGAAGTGGTTGGTACGGGCT
CGATCCGGTCGCCGCTGACTTGTTCATCTTCGGGAAGGTGATGAGCGGCGGAATGCCGGCCGCTGCGTTCGGCGGGCGAG
CTGAGGTGATGGAGAGGCTGGCGCCGTTGGGGCCGGTGTATCAGGCGGGGACTCTGTCGGGTAACCCGGTGGCGATGGCC
GCCGGGCTGGCAACGCTACGGGCTGCCGCCGACGCAGTTTATGCTACACTGGACCGCAACGCCGACCGGTTGGTTGCAAT
GCTGTCTGAGGCGCTTACGGATGCCGGTGTTCCACATCAGATTCCGCGTGCCGGCAATATGTTCAGCGTGTTCTTTTCCG
AAGCGCCTGTGACCGACTTTGCGTCCGCTTGCAACAGCCAGGTCTGGCGTTATCCGGCGTTTTTTCATGCCTTGCTGGAC
GCGGGCGTCTACCCGCCGTGCAGTACGTTCGAGGCGTGGTTTGTCTCGGCCGCTCTCGACGACGCCGCGTTCGGTCGCAT
CGTTGATGCTCTGCCCGGTGCGGCCGCTGCCGCAGTCGCCGCCCGGCATCGGGAGTCCTGA

Upstream 100 bases:

>100_bases
ATGTCGTTATCCAACTTGTCGACTTGGGCCGACCTAAAGTTGCGCCGAATTCTTACACCCTGTAGTTCAACGAGTCGTGG
AGGCTGGAACACTGGTAATC

Downstream 100 bases:

>100_bases
TGGCCGAACATAATCGGGTCCATGTGGTGCGTCACGGTGAGGTGGACAACCCCACCGGGATCCTCTACGGTCGACTCGCC
GGGTTTGGCCTGTCTGAGAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MGSTDQATAPAGPAVSISAKLFEDACAVIPGGVNSPVRAFSAVGGTPLFITEARGCWLTDADGRRYVDLVCSWGPMILGH
AHPAVVDAVATVAASGLSFGAPTPAETQLAAEIIGRMAPVERIRLVNSGTEATMSAVRLARGFTGRTKIIKFSGCYHGHV
DALLADAGSGVATLSLPSSPGVTGAAAADTIVLPYNDIEAVRQTFARLGDQIAAVITEASPGNMGVVPPAPGYNAALRAI
TAEHGALLIIDEVMTGFRVSRSGWYGLDPVAADLFIFGKVMSGGMPAAAFGGRAEVMERLAPLGPVYQAGTLSGNPVAMA
AGLATLRAAADAVYATLDRNADRLVAMLSEALTDAGVPHQIPRAGNMFSVFFSEAPVTDFASACNSQVWRYPAFFHALLD
AGVYPPCSTFEAWFVSAALDDAAFGRIVDALPGAAAAAVAARHRES

Sequences:

>Translated_446_residues
MGSTDQATAPAGPAVSISAKLFEDACAVIPGGVNSPVRAFSAVGGTPLFITEARGCWLTDADGRRYVDLVCSWGPMILGH
AHPAVVDAVATVAASGLSFGAPTPAETQLAAEIIGRMAPVERIRLVNSGTEATMSAVRLARGFTGRTKIIKFSGCYHGHV
DALLADAGSGVATLSLPSSPGVTGAAAADTIVLPYNDIEAVRQTFARLGDQIAAVITEASPGNMGVVPPAPGYNAALRAI
TAEHGALLIIDEVMTGFRVSRSGWYGLDPVAADLFIFGKVMSGGMPAAAFGGRAEVMERLAPLGPVYQAGTLSGNPVAMA
AGLATLRAAADAVYATLDRNADRLVAMLSEALTDAGVPHQIPRAGNMFSVFFSEAPVTDFASACNSQVWRYPAFFHALLD
AGVYPPCSTFEAWFVSAALDDAAFGRIVDALPGAAAAAVAARHRES
>Mature_445_residues
GSTDQATAPAGPAVSISAKLFEDACAVIPGGVNSPVRAFSAVGGTPLFITEARGCWLTDADGRRYVDLVCSWGPMILGHA
HPAVVDAVATVAASGLSFGAPTPAETQLAAEIIGRMAPVERIRLVNSGTEATMSAVRLARGFTGRTKIIKFSGCYHGHVD
ALLADAGSGVATLSLPSSPGVTGAAAADTIVLPYNDIEAVRQTFARLGDQIAAVITEASPGNMGVVPPAPGYNAALRAIT
AEHGALLIIDEVMTGFRVSRSGWYGLDPVAADLFIFGKVMSGGMPAAAFGGRAEVMERLAPLGPVYQAGTLSGNPVAMAA
GLATLRAAADAVYATLDRNADRLVAMLSEALTDAGVPHQIPRAGNMFSVFFSEAPVTDFASACNSQVWRYPAFFHALLDA
GVYPPCSTFEAWFVSAALDDAAFGRIVDALPGAAAAAVAARHRES

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=322, Percent_Identity=27.9503105590062, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI37574042, Length=337, Percent_Identity=28.486646884273, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI13994255, Length=346, Percent_Identity=27.1676300578035, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI24119277, Length=336, Percent_Identity=26.7857142857143, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226442705, Length=336, Percent_Identity=27.3809523809524, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI226442709, Length=306, Percent_Identity=28.7581699346405, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI284507298, Length=239, Percent_Identity=29.2887029288703, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786349, Length=421, Percent_Identity=52.9691211401425, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1789016, Length=303, Percent_Identity=32.6732673267327, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693181, Length=288, Percent_Identity=32.2916666666667, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789759, Length=288, Percent_Identity=31.25, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787560, Length=382, Percent_Identity=31.413612565445, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788044, Length=288, Percent_Identity=30.5555555555556, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71992977, Length=348, Percent_Identity=28.1609195402299, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25144271, Length=295, Percent_Identity=27.1186440677966, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323470, Length=305, Percent_Identity=25.2459016393443, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324432, Length=356, Percent_Identity=26.685393258427, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356575, Length=308, Percent_Identity=29.2207792207792, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357415, Length=322, Percent_Identity=25.4658385093168, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_MYCLB (B8ZT67)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504193.1
- ProteinModelPortal:   B8ZT67
- SMR:   B8ZT67
- EnsemblBacteria:   EBMYCT00000087685
- GeneID:   7324736
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015339
- HOGENOM:   HBG725944
- OMA:   NENAVRD
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45791; Mature: 45659

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSTDQATAPAGPAVSISAKLFEDACAVIPGGVNSPVRAFSAVGGTPLFITEARGCWLTD
CCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCEEEC
ADGRRYVDLVCSWGPMILGHAHPAVVDAVATVAASGLSFGAPTPAETQLAAEIIGRMAPV
CCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCH
ERIRLVNSGTEATMSAVRLARGFTGRTKIIKFSGCYHGHVDALLADAGSGVATLSLPSSP
HHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCC
GVTGAAAADTIVLPYNDIEAVRQTFARLGDQIAAVITEASPGNMGVVPPAPGYNAALRAI
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHH
TAEHGALLIIDEVMTGFRVSRSGWYGLDPVAADLFIFGKVMSGGMPAAAFGGRAEVMERL
HHCCCCEEEEHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
APLGPVYQAGTLSGNPVAMAAGLATLRAAADAVYATLDRNADRLVAMLSEALTDAGVPHQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
IPRAGNMFSVFFSEAPVTDFASACNSQVWRYPAFFHALLDAGVYPPCSTFEAWFVSAALD
CCCCCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DAAFGRIVDALPGAAAAAVAARHRES
HHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GSTDQATAPAGPAVSISAKLFEDACAVIPGGVNSPVRAFSAVGGTPLFITEARGCWLTD
CCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCEEEC
ADGRRYVDLVCSWGPMILGHAHPAVVDAVATVAASGLSFGAPTPAETQLAAEIIGRMAPV
CCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCH
ERIRLVNSGTEATMSAVRLARGFTGRTKIIKFSGCYHGHVDALLADAGSGVATLSLPSSP
HHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCC
GVTGAAAADTIVLPYNDIEAVRQTFARLGDQIAAVITEASPGNMGVVPPAPGYNAALRAI
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHH
TAEHGALLIIDEVMTGFRVSRSGWYGLDPVAADLFIFGKVMSGGMPAAAFGGRAEVMERL
HHCCCCEEEEHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
APLGPVYQAGTLSGNPVAMAAGLATLRAAADAVYATLDRNADRLVAMLSEALTDAGVPHQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
IPRAGNMFSVFFSEAPVTDFASACNSQVWRYPAFFHALLDAGVYPPCSTFEAWFVSAALD
CCCCCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
DAAFGRIVDALPGAAAAAVAARHRES
HHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA