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The map label for this gene is tsnR [H]

Identifier: 221230209

GI number: 221230209

Start: 1677040

End: 1677819

Strand: Direct

Name: tsnR [H]

Synonym: MLBr_01397

Alternate gene names: 221230209

Gene position: 1677040-1677819 (Clockwise)

Preceding gene: 221230208

Following gene: 221230210

Centisome position: 51.32

GC content: 60.77

Gene sequence:

>780_bases
GTGCTCACCGAGCGTTCCGCCCGGGTGGTCGCGGCAGTTAAGTTGCATCGCCAAGTCGCGCGGCGGCGTGTCAGACGATT
TCTCGCTGAAGGCCCTAACCTCGTCGAAGCCGCCATTGTAAGCGGGCTGGTTCGCGACGTATTCGTCACAGAAGTCGCGG
CCCAACGGCACGCCACAGTGCTGGCTACACTGAAGTGTCCGGTCCATCCTGTAAGCGAACGTGCCGCAAAAGCACTTTCC
GACACGGTCACTCCGACTGGGTTGATCGCGGTGTGCGAAATGCCGACGACCCGGCTAGCGACTGTGCTGGCTGGCTCACC
CCAGCTGGTGGTGGCCGCTGTCGAGATCAGCGAACCGGGCAATGCGGGCACTGTAATCCGCATCGCCGATGCCATGGGTG
CTGCAGCTGTGATCTTCGGTGGGTACACCGTCGACCCGTACAACGGTAAATGTCTGCGTTCCTCTGCCGGCAGTATCTTC
TCGATAACGGTTGTCGTGGCGTCTGATGCCTACGCCGCTGTGACCGAATTGCGCGCCGCCGGACTGCAAGTGCTGGCCAC
CATGGTCGACGGTGAGACGTCTCTCGACGAAGCCAAGCCACTGCTTGGCAAGCCGACAGCTTGGCTTTTCGGGTCCGAAC
CACACGGTTTGTCAACCGAAATCGCAGAAGCAGCGGACTATAGAGTGCGTATTCCGATGGTCGGCGGTGTGGAAAGCCTG
AACGTGGCTGCGACCGCAGCGATTTGCCTCTATCAGAGCTCGCGTGCATTGCGTCTTTAG

Upstream 100 bases:

>100_bases
GGGGTTACCCGAAGATGTCAACGTGCCATCCGGAGAAGCTGCTTAACCCACCACGATGCGCACCAAGGAGCGGGGAAATC
GAACCTTGCCGGGGCCTGCG

Downstream 100 bases:

>100_bases
CCATCTGGTGGTACAAGTCACGACTCCTGGCTTAGCCGCACTGGCCAGATCGCCACTAGCGCGTTCATTCCTGGCCTTAG
GCAATCTTTTCCTACGGGGC

Product: putative rRNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLTERSARVVAAVKLHRQVARRRVRRFLAEGPNLVEAAIVSGLVRDVFVTEVAAQRHATVLATLKCPVHPVSERAAKALS
DTVTPTGLIAVCEMPTTRLATVLAGSPQLVVAAVEISEPGNAGTVIRIADAMGAAAVIFGGYTVDPYNGKCLRSSAGSIF
SITVVVASDAYAAVTELRAAGLQVLATMVDGETSLDEAKPLLGKPTAWLFGSEPHGLSTEIAEAADYRVRIPMVGGVESL
NVAATAAICLYQSSRALRL

Sequences:

>Translated_259_residues
MLTERSARVVAAVKLHRQVARRRVRRFLAEGPNLVEAAIVSGLVRDVFVTEVAAQRHATVLATLKCPVHPVSERAAKALS
DTVTPTGLIAVCEMPTTRLATVLAGSPQLVVAAVEISEPGNAGTVIRIADAMGAAAVIFGGYTVDPYNGKCLRSSAGSIF
SITVVVASDAYAAVTELRAAGLQVLATMVDGETSLDEAKPLLGKPTAWLFGSEPHGLSTEIAEAADYRVRIPMVGGVESL
NVAATAAICLYQSSRALRL
>Mature_259_residues
MLTERSARVVAAVKLHRQVARRRVRRFLAEGPNLVEAAIVSGLVRDVFVTEVAAQRHATVLATLKCPVHPVSERAAKALS
DTVTPTGLIAVCEMPTTRLATVLAGSPQLVVAAVEISEPGNAGTVIRIADAMGAAAVIFGGYTVDPYNGKCLRSSAGSIF
SITVVVASDAYAAVTELRAAGLQVLATMVDGETSLDEAKPLLGKPTAWLFGSEPHGLSTEIAEAADYRVRIPMVGGVESL
NVAATAAICLYQSSRALRL

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=177, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24666840, Length=271, Percent_Identity=26.1992619926199, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 27060; Mature: 27060

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTERSARVVAAVKLHRQVARRRVRRFLAEGPNLVEAAIVSGLVRDVFVTEVAAQRHATV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEE
LATLKCPVHPVSERAAKALSDTVTPTGLIAVCEMPTTRLATVLAGSPQLVVAAVEISEPG
EEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCC
NAGTVIRIADAMGAAAVIFGGYTVDPYNGKCLRSSAGSIFSITVVVASDAYAAVTELRAA
CCCCEEEEECCCCCEEEEECCEEECCCCCEEEECCCCCEEEEEEEEECCHHHHHHHHHHH
GLQVLATMVDGETSLDEAKPLLGKPTAWLFGSEPHGLSTEIAEAADYRVRIPMVGGVESL
HHHHHHHHHCCCCCHHHCCCCCCCCCEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCHH
NVAATAAICLYQSSRALRL
HHHHHHHHEEEECCCCCCC
>Mature Secondary Structure
MLTERSARVVAAVKLHRQVARRRVRRFLAEGPNLVEAAIVSGLVRDVFVTEVAAQRHATV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEE
LATLKCPVHPVSERAAKALSDTVTPTGLIAVCEMPTTRLATVLAGSPQLVVAAVEISEPG
EEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCC
NAGTVIRIADAMGAAAVIFGGYTVDPYNGKCLRSSAGSIFSITVVVASDAYAAVTELRAA
CCCCEEEEECCCCCEEEEECCEEECCCCCEEEECCCCCEEEEEEEEECCHHHHHHHHHHH
GLQVLATMVDGETSLDEAKPLLGKPTAWLFGSEPHGLSTEIAEAADYRVRIPMVGGVESL
HHHHHHHHHCCCCCHHHCCCCCCCCCEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCHH
NVAATAAICLYQSSRALRL
HHHHHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]