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The map label for this gene is tyrS

Identifier: 221230180

GI number: 221230180

Start: 1612108

End: 1613388

Strand: Direct

Name: tyrS

Synonym: MLBr_01352

Alternate gene names: 221230180

Gene position: 1612108-1613388 (Clockwise)

Preceding gene: 221230179

Following gene: 221230181

Centisome position: 49.33

GC content: 60.5

Gene sequence:

>1281_bases
GTGTCTTCCAGCATCCTTAACGAGCTGGGCTGGCGTGGGTTGGTCGCGCAGTCCACTGACCTCGATGCTCTAGCCGACGA
GTTGCGGCGTGGACCGATGACAGTCTACGCCGGTTTCGACCCCACCGCGCCGAGCCTGCATGTCGGACACTTGGTGCCGC
TGCTGGTATTGCGGCGCTTTCAACGAGCCGGCCACCGGCCAATCGTGCTAGCGGGCGGGGCAACCGGCATGATTGGTGAT
CCGCGCGACATCGGTGAGCGCACCCTCAACGAGGCGTACACCGTCGCCGAATGGGCCGAGCGAATCCGCGGACAGCTGGA
GCGTTTCGTCGAATTCGACGATGTCGCGAAGTCTAGGAACGGCGCCATCGTTGTCAACAACCTGGAATGGACGAGCCCGA
TGTCGGCGATCGAGTTCTTGCGCGATGTCGGCAAACACTTTTCGATCAACTTGATGCTAGATCGGGACACAATCCGGCGA
CGGTTGAATGGCCAAGGGATTTCTTACACGGAGTTCAGCTACATGCTGTTGCAAGCCAACGATTATGCTGAATTGCATCA
GCGCCATGGCTGTGCGCTGCAGATCGGCGGTTCGGATCAGTGGGGCAACATCATCGCCGGGGTTCGCCTGGTGCGACAAA
AGCTCGGCGCCACAGTGCATGCGCTCACAGTCCCGCTGGTGACCGCTGCCGATGGCGCCAAGCTCGGCAAATCCACCGGA
GGTGGCAGTTTATGGTTGGACCCAGAGATGACCAGCCCGTACGCCTGGTACCAATACTTCATCAACACCTCCGACGCTGA
TGTCATTCGGTACCTGCGTTGGTTCACTTTTTTGTTGCCCGAAGAACTGGTCGAGCTGGAGCAGACGACGGTCTCACGCC
CACAGGAACGTGCTGCACAACGTCGACTCGCCACCGAGCTCACGGTTTTGGTGCATGGCGCAGCGGCAACCCAGGCTGTT
GAGCATGCCAGCAGGGCATTGTTCGGTCAGGGCGAACTTGCTCGCCTTGACGAAGCGACGTTGGCGACGGCATTGCGCGA
GACAGTGGTCGCTGAGCTGAAACCCGGCAATCCCGATGGTATTGTCGATCTGTTGGTGGCCAGCGGTCTGTCCCCTAGCA
GGGGTGCGGCGCGGCGCACTATCGATGAAGGCGGGGTGTTGGTCAACAACATTCGCATCCAGAGCGAGGAATGGACGCCC
CGGACATCGGATTTCCTGCACGGCCGCTGGCTGGTGCTGCGCCGTGGCAAGCGGAACATCGCCGGGATAGAAAGAGTATA
G

Upstream 100 bases:

>100_bases
CGACCCTGGCGATTGTGGCTGCCGGGCCGACCCGAAGTCTCGACTTATCGTCGAAGTCCCCGAGCTCCGGCTCCCGGTGC
TAGCGACTAGCCTCTTTTGG

Downstream 100 bases:

>100_bases
CACGGGTTGGGTGATCGCTAGTTTACTAGGCCCCAGATAGCCCGATTTGGATGGTCGGCAGCTGGTTGTCCACGGCAGCT
ACAGCGGGCGGGGCGGTGGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSSSILNELGWRGLVAQSTDLDALADELRRGPMTVYAGFDPTAPSLHVGHLVPLLVLRRFQRAGHRPIVLAGGATGMIGD
PRDIGERTLNEAYTVAEWAERIRGQLERFVEFDDVAKSRNGAIVVNNLEWTSPMSAIEFLRDVGKHFSINLMLDRDTIRR
RLNGQGISYTEFSYMLLQANDYAELHQRHGCALQIGGSDQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGAKLGKSTG
GGSLWLDPEMTSPYAWYQYFINTSDADVIRYLRWFTFLLPEELVELEQTTVSRPQERAAQRRLATELTVLVHGAAATQAV
EHASRALFGQGELARLDEATLATALRETVVAELKPGNPDGIVDLLVASGLSPSRGAARRTIDEGGVLVNNIRIQSEEWTP
RTSDFLHGRWLVLRRGKRNIAGIERV

Sequences:

>Translated_426_residues
MSSSILNELGWRGLVAQSTDLDALADELRRGPMTVYAGFDPTAPSLHVGHLVPLLVLRRFQRAGHRPIVLAGGATGMIGD
PRDIGERTLNEAYTVAEWAERIRGQLERFVEFDDVAKSRNGAIVVNNLEWTSPMSAIEFLRDVGKHFSINLMLDRDTIRR
RLNGQGISYTEFSYMLLQANDYAELHQRHGCALQIGGSDQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGAKLGKSTG
GGSLWLDPEMTSPYAWYQYFINTSDADVIRYLRWFTFLLPEELVELEQTTVSRPQERAAQRRLATELTVLVHGAAATQAV
EHASRALFGQGELARLDEATLATALRETVVAELKPGNPDGIVDLLVASGLSPSRGAARRTIDEGGVLVNNIRIQSEEWTP
RTSDFLHGRWLVLRRGKRNIAGIERV
>Mature_425_residues
SSSILNELGWRGLVAQSTDLDALADELRRGPMTVYAGFDPTAPSLHVGHLVPLLVLRRFQRAGHRPIVLAGGATGMIGDP
RDIGERTLNEAYTVAEWAERIRGQLERFVEFDDVAKSRNGAIVVNNLEWTSPMSAIEFLRDVGKHFSINLMLDRDTIRRR
LNGQGISYTEFSYMLLQANDYAELHQRHGCALQIGGSDQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGAKLGKSTGG
GSLWLDPEMTSPYAWYQYFINTSDADVIRYLRWFTFLLPEELVELEQTTVSRPQERAAQRRLATELTVLVHGAAATQAVE
HASRALFGQGELARLDEATLATALRETVVAELKPGNPDGIVDLLVASGLSPSRGAARRTIDEGGVLVNNIRIQSEEWTPR
TSDFLHGRWLVLRRGKRNIAGIERV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=405, Percent_Identity=38.0246913580247, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI1787925, Length=426, Percent_Identity=48.3568075117371, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145802, Length=359, Percent_Identity=35.933147632312, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25145805, Length=287, Percent_Identity=33.4494773519164, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=450, Percent_Identity=33.7777777777778, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI20130383, Length=439, Percent_Identity=37.5854214123007, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_MYCLE (Q49900)

Other databases:

- EMBL:   Z95117
- EMBL:   AL583921
- EMBL:   U00021
- PIR:   B87078
- PIR:   S72970
- RefSeq:   NP_301966.1
- ProteinModelPortal:   Q49900
- SMR:   Q49900
- EnsemblBacteria:   EBMYCT00000028211
- GeneID:   910480
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1352
- NMPDR:   fig|272631.1.peg.838
- Leproma:   ML1352
- GeneTree:   EBGT00050000017241
- HOGENOM:   HBG288125
- OMA:   DRIDNQL
- ProtClustDB:   PRK05912
- BioCyc:   MLEP272631:ML1352-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47050; Mature: 46919

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 36-36 BINDING 174-174 BINDING 178-178 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSILNELGWRGLVAQSTDLDALADELRRGPMTVYAGFDPTAPSLHVGHLVPLLVLRRF
CCCHHHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
QRAGHRPIVLAGGATGMIGDPRDIGERTLNEAYTVAEWAERIRGQLERFVEFDDVAKSRN
HHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAIVVNNLEWTSPMSAIEFLRDVGKHFSINLMLDRDTIRRRLNGQGISYTEFSYMLLQAN
CEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCEEHEEEEEEECC
DYAELHQRHGCALQIGGSDQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGAKLGKSTG
CHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEHHHHEEECCCCHHCCCCCC
GGSLWLDPEMTSPYAWYQYFINTSDADVIRYLRWFTFLLPEELVELEQTTVSRPQERAAQ
CCCEEECCCCCCCCEEEHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RRLATELTVLVHGAAATQAVEHASRALFGQGELARLDEATLATALRETVVAELKPGNPDG
HHHHHHHHHEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
IVDLLVASGLSPSRGAARRTIDEGGVLVNNIRIQSEEWTPRTSDFLHGRWLVLRRGKRNI
HHHHHHHCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHCCEEEEEECCCCCC
AGIERV
CCCCCC
>Mature Secondary Structure 
SSSILNELGWRGLVAQSTDLDALADELRRGPMTVYAGFDPTAPSLHVGHLVPLLVLRRF
CCHHHHHHCCCCEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
QRAGHRPIVLAGGATGMIGDPRDIGERTLNEAYTVAEWAERIRGQLERFVEFDDVAKSRN
HHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAIVVNNLEWTSPMSAIEFLRDVGKHFSINLMLDRDTIRRRLNGQGISYTEFSYMLLQAN
CEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCEEHEEEEEEECC
DYAELHQRHGCALQIGGSDQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGAKLGKSTG
CHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEHHHHEEECCCCHHCCCCCC
GGSLWLDPEMTSPYAWYQYFINTSDADVIRYLRWFTFLLPEELVELEQTTVSRPQERAAQ
CCCEEECCCCCCCCEEEHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RRLATELTVLVHGAAATQAVEHASRALFGQGELARLDEATLATALRETVVAELKPGNPDG
HHHHHHHHHEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
IVDLLVASGLSPSRGAARRTIDEGGVLVNNIRIQSEEWTPRTSDFLHGRWLVLRRGKRNI
HHHHHHHCCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHCCEEEEEECCCCCC
AGIERV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002