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The map label for this gene is pykA [H]

Identifier: 221230136

GI number: 221230136

Start: 1519765

End: 1521183

Strand: Direct

Name: pykA [H]

Synonym: MLBr_01277

Alternate gene names: 221230136

Gene position: 1519765-1521183 (Clockwise)

Preceding gene: 221230135

Following gene: 221230137

Centisome position: 46.5

GC content: 60.68

Gene sequence:

>1419_bases
GTGACGAGACGCGGGAAGATCGTCTGTACCCTTGGCCCTGCAACACAATCGGACGAGTTAATCCGGGCGCTGGTTGAAGC
CGGAATGGACGTTGCCCGCATGAACTTCAGTCACGGCGATTACTCCGATCACAAGGCTGTTTACGATCGGGTGCGCGTCG
CATCTGATGCAACCGGACGCGCGGTCGGCGTGCTCGCTGACCTGCAGGGCCCAAAGATCAGGCTGGGACGCTTTGCCACG
GGGTCCACCTACTGGGCTGACGGTGAAACGGTAAGGATCACTGTGGCCGACTGCCACGGTAGCCACGACCGGGTATCAAC
CACCTACAAGAATTTGGCCAGGGATGCGGTGGTCGGTGACCGGGTTCTGGTCGACGACGGCAAGGTCGGACTGGTGGTCT
CGGCCATCGATGGCGACGACGTGGTCTGTACTGTCACTGAAGGCGGCCCGGTTAGCAACAACAAGGGCATGTCGTTGCCC
GGGATGCACGTGTCTGCTCCGGCTTTGTCGGACAAGGACATCGAGGACCTCACGTTCGCGCTGAATCTCGGCGCCGATCT
GGTCGCGCTGTCGTTTGTGCGCTCGCCGTCTGACGTTGAGCTGGTCCACAAGGCGATGGATCGCGTCGGGCGGCGTGTAC
CGGTGATCGCCAAGCTGGAGAAGCCAGAAGCCGTCGATAACCTCGAAGCTATTGTGTTGGCATTCGATGCCATCATGGTG
GCTCGCGGCGACCTGGGTGTCGAGCTGCCACTTGAAGAGGTCCCACTGGTGCAGAAGCGGGCAATCCAGATGGCAAGGGA
GAATGCGAAACCCGTCATCGTGGCTACCCAGATGCTAGACTCGATGATCGAAAACTCCCGGCCGACCCGAGCTGAGGCCT
CCGACGTCGCTAATGCGGTGCTCGACGGCGCCGACGCGCTCATGTTGTCTGAGGAAACGTCGGTGGGGAAGTACCCATTG
GCAGCCGTGAAGACGATGTCGCGCATTGTGTGTGCGGTTGAGGAGAATTCCACGGCCGCGCCGCCGTTGACACACGTACC
TCGCACGAAGCGGGGGGTGATCTCGTACGCTGCCCGCGATATCGGTGAAAGACTCGACGCTAAGGCCTTGGTTGCGTTCA
CTCAATCCGGTGACACGGTGCGGCGACTGGCGCGTCTACATACCCCGCTGCCGCTGCTTGCCTTCACCGCGTGGCCAGAG
GTGCGCAGCCAACTCGCTATGACTTGGGGCACCGAGACATTTCTCGTACCAAAGATGCTGTCCACTGACGGTATGATACG
CCAGGTGGACAAGTCACTGCTCGAACTTGGTCGCTACAAGCGTGGTGACTTGGTGGTGATCGTCGCTGGCACGCCACCTG
GCACAGTAGGATCGACCAACTTGATCCATGTGCACCGGATTGGGGAGGACGACGTATAG

Upstream 100 bases:

>100_bases
ACTGTTTACAGCGGGTAACCGTGCGGTGCGCCGGACAAGTTAGCCGTCCTTACGTACCTTAAATCCGCGCTGGTTATTGG
CGAGACTATGCTGGGTCGTC

Downstream 100 bases:

>100_bases
GTCGCCCGTCTCGGCAACGATGCGGGCCGGGTAGCGGCCCGACGAAGAGCTGGGCAATCAGTCTGAGGAGAGCCCGTCCG
TGTCGGTTTGGCGATGAGCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTRRGKIVCTLGPATQSDELIRALVEAGMDVARMNFSHGDYSDHKAVYDRVRVASDATGRAVGVLADLQGPKIRLGRFAT
GSTYWADGETVRITVADCHGSHDRVSTTYKNLARDAVVGDRVLVDDGKVGLVVSAIDGDDVVCTVTEGGPVSNNKGMSLP
GMHVSAPALSDKDIEDLTFALNLGADLVALSFVRSPSDVELVHKAMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSEETSVGKYPL
AAVKTMSRIVCAVEENSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPE
VRSQLAMTWGTETFLVPKMLSTDGMIRQVDKSLLELGRYKRGDLVVIVAGTPPGTVGSTNLIHVHRIGEDDV

Sequences:

>Translated_472_residues
MTRRGKIVCTLGPATQSDELIRALVEAGMDVARMNFSHGDYSDHKAVYDRVRVASDATGRAVGVLADLQGPKIRLGRFAT
GSTYWADGETVRITVADCHGSHDRVSTTYKNLARDAVVGDRVLVDDGKVGLVVSAIDGDDVVCTVTEGGPVSNNKGMSLP
GMHVSAPALSDKDIEDLTFALNLGADLVALSFVRSPSDVELVHKAMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSEETSVGKYPL
AAVKTMSRIVCAVEENSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPE
VRSQLAMTWGTETFLVPKMLSTDGMIRQVDKSLLELGRYKRGDLVVIVAGTPPGTVGSTNLIHVHRIGEDDV
>Mature_471_residues
TRRGKIVCTLGPATQSDELIRALVEAGMDVARMNFSHGDYSDHKAVYDRVRVASDATGRAVGVLADLQGPKIRLGRFATG
STYWADGETVRITVADCHGSHDRVSTTYKNLARDAVVGDRVLVDDGKVGLVVSAIDGDDVVCTVTEGGPVSNNKGMSLPG
MHVSAPALSDKDIEDLTFALNLGADLVALSFVRSPSDVELVHKAMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMVA
RGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSEETSVGKYPLA
AVKTMSRIVCAVEENSTAAPPLTHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEV
RSQLAMTWGTETFLVPKMLSTDGMIRQVDKSLLELGRYKRGDLVVIVAGTPPGTVGSTNLIHVHRIGEDDV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=37.1951219512195, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=36.8098159509202, Blast_Score=285, Evalue=9e-77,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=36.8098159509202, Blast_Score=285, Evalue=9e-77,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=37.1196754563895, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=37.1196754563895, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=38.3275261324042, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=38.3275261324042, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=35.7446808510638, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=35.7446808510638, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.0467289719626, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=40.8510638297872, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=38.7029288702929, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=34.2213114754098, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=37.037037037037, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=36.8312757201646, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=36.8312757201646, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=37.037037037037, Blast_Score=271, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=34.5755693581781, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=35.3182751540041, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=36.530612244898, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=36.530612244898, Blast_Score=277, Evalue=9e-75,
Organism=Drosophila melanogaster, GI24648966, Length=461, Percent_Identity=33.4056399132321, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=27.5720164609054, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24646914, Length=286, Percent_Identity=32.8671328671329, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50655; Mature: 50524

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRGKIVCTLGPATQSDELIRALVEAGMDVARMNFSHGDYSDHKAVYDRVRVASDATGR
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFATGSTYWADGETVRITVADCHGSHDRVSTTYKNLARDAVVGD
EEEEEEECCCCCEEEEEEECCCEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
RVLVDDGKVGLVVSAIDGDDVVCTVTEGGPVSNNKGMSLPGMHVSAPALSDKDIEDLTFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHHEEEE
LNLGADLVALSFVRSPSDVELVHKAMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
EECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADALMLSEETSVGKYPLAAVKTMSRIVCAVEENSTAAPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEVRSQLAMTWGTETFLVPKML
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCHHHEECCHHHHHHEEHHCCCCEEEECHHH
STDGMIRQVDKSLLELGRYKRGDLVVIVAGTPPGTVGSTNLIHVHRIGEDDV
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TRRGKIVCTLGPATQSDELIRALVEAGMDVARMNFSHGDYSDHKAVYDRVRVASDATGR
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
AVGVLADLQGPKIRLGRFATGSTYWADGETVRITVADCHGSHDRVSTTYKNLARDAVVGD
EEEEEEECCCCCEEEEEEECCCEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCC
RVLVDDGKVGLVVSAIDGDDVVCTVTEGGPVSNNKGMSLPGMHVSAPALSDKDIEDLTFA
EEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCHHHEEEE
LNLGADLVALSFVRSPSDVELVHKAMDRVGRRVPVIAKLEKPEAVDNLEAIVLAFDAIMV
EECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHE
ARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVATQMLDSMIENSRPTRAEASDVANAV
ECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHHHH
LDGADALMLSEETSVGKYPLAAVKTMSRIVCAVEENSTAAPPLTHVPRTKRGVISYAARD
HCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
IGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEVRSQLAMTWGTETFLVPKML
HHHHCCCEEEEEEECCCHHHHHHHHHCCCCCHHHEECCHHHHHHEEHHCCCCEEEECHHH
STDGMIRQVDKSLLELGRYKRGDLVVIVAGTPPGTVGSTNLIHVHRIGEDDV
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA