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The map label for this gene is mdh

Identifier: 221230013

GI number: 221230013

Start: 1254711

End: 1255700

Strand: Direct

Name: mdh

Synonym: MLBr_01091

Alternate gene names: 221230013

Gene position: 1254711-1255700 (Clockwise)

Preceding gene: 221230011

Following gene: 221230014

Centisome position: 38.39

GC content: 63.74

Gene sequence:

>990_bases
GTGAGCCCACGTCCCCTCAAGGTCGCCGTAACCGGCGCTGCCGGTCAGATCGGTTATAGCCTGCTGTTCCGCTTGGCGAG
CGGCTCACTGCTGGGGCTTGACCGCCCGATTGAGTTGCGTCTGCTTGAGATTGAGCCCGCGCTCAAGGCGCTCGAGGGTG
TCGTGATGGAACTTGATGACTGCGCGTTCCTGCTGTTGGCGGGCGTCGAGATAGGCGCGGATCCGAACAAGGTCTTCGAC
GGTGTCAACCTGGCCCTGCTTGTCGGTGCCCGGCCGCGCGGGCCGGGCATGGAACGTGGCGATTTATTGGAGGCTAACGG
CGCGATATTCACTGCGCAGGGCAAGGCCCTCAACGCCGTCGCCGCCGCTGACATCCGTGTCGGTGTGACTGGCAATCCGG
CCAACACCAACGCGCTGATCGCGATGACCAACGCGCCCGACATCCCCCGCGAGCGGTTCTCGGCACTTACCCGTCTGGAC
CACAACCGGGCTATTGCGCAGCTAGCCACGAAGACCGGCTCCGCAGTCACCGACATCAGGAAGATGACGATCTGGGGCAA
CCACTCGGCCACCCAATACCCCGACGTTTTCCACGCTGAGATCGGCGGTAAGAACGCGGCCGAGGTCGTGGGGGACCAAG
CTTGGATCGAGGACTACTTTATCCCGACCGTCGCCAAGCGCGGTGCTGCGATCATCGATGCACGCGGCGCTTCGTCAGCC
GCTTCGGCAGCATCGGCGACCATTGATGCCGCCCGGGACTGGCTGCTGGGCACCCCTGAGGGCAACTGGGTTTCGATGGC
GGTGGTATCCGATGGTTCGTACGGTGTGCCGGAGGGCCTGATCTCTTCGTTCCCTGTAACCACCACGGATGGAGACTGGA
CGATCGTCAGGGGCTTGGGGATCGATGACTTCTCCCGCGGGCGGATCGACAAGTCGACCGCAGAGCTGGCCGACGAGCGT
ATGGCGGTCAAGCAGCTCGGTCTGATTTAG

Upstream 100 bases:

>100_bases
CGAAAATCTCGGGTCGGTAAGGATGCGCTAGTTTCCATCCAAGCTTGAAACTCGTACCGATAGCTCGAGCCGAACCTCTC
TCCGATAAGGAGTAATCGAA

Downstream 100 bases:

>100_bases
GGGCGGTTCCGCGCTAGACGTCTGTCACCCGTCGTGGCCGAGACTACCGGAGGTTCTCGGCGTTCCCCGGTTCCGTCTCT
CAGGCCGTCGTTGCCGATAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSPRPLKVAVTGAAGQIGYSLLFRLASGSLLGLDRPIELRLLEIEPALKALEGVVMELDDCAFLLLAGVEIGADPNKVFD
GVNLALLVGARPRGPGMERGDLLEANGAIFTAQGKALNAVAAADIRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLD
HNRAIAQLATKTGSAVTDIRKMTIWGNHSATQYPDVFHAEIGGKNAAEVVGDQAWIEDYFIPTVAKRGAAIIDARGASSA
ASAASATIDAARDWLLGTPEGNWVSMAVVSDGSYGVPEGLISSFPVTTTDGDWTIVRGLGIDDFSRGRIDKSTAELADER
MAVKQLGLI

Sequences:

>Translated_329_residues
MSPRPLKVAVTGAAGQIGYSLLFRLASGSLLGLDRPIELRLLEIEPALKALEGVVMELDDCAFLLLAGVEIGADPNKVFD
GVNLALLVGARPRGPGMERGDLLEANGAIFTAQGKALNAVAAADIRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLD
HNRAIAQLATKTGSAVTDIRKMTIWGNHSATQYPDVFHAEIGGKNAAEVVGDQAWIEDYFIPTVAKRGAAIIDARGASSA
ASAASATIDAARDWLLGTPEGNWVSMAVVSDGSYGVPEGLISSFPVTTTDGDWTIVRGLGIDDFSRGRIDKSTAELADER
MAVKQLGLI
>Mature_328_residues
SPRPLKVAVTGAAGQIGYSLLFRLASGSLLGLDRPIELRLLEIEPALKALEGVVMELDDCAFLLLAGVEIGADPNKVFDG
VNLALLVGARPRGPGMERGDLLEANGAIFTAQGKALNAVAAADIRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLDH
NRAIAQLATKTGSAVTDIRKMTIWGNHSATQYPDVFHAEIGGKNAAEVVGDQAWIEDYFIPTVAKRGAAIIDARGASSAA
SAASATIDAARDWLLGTPEGNWVSMAVVSDGSYGVPEGLISSFPVTTTDGDWTIVRGLGIDDFSRGRIDKSTAELADERM
AVKQLGLI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=323, Percent_Identity=52.6315789473684, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI89886456, Length=331, Percent_Identity=24.4712990936556, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561064, Length=322, Percent_Identity=54.0372670807453, Blast_Score=322, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32566798, Length=260, Percent_Identity=52.6923076923077, Blast_Score=244, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=55.2941176470588, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24583394, Length=321, Percent_Identity=50.1557632398754, Blast_Score=305, Evalue=2e-83,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_MYCLE (P50917)

Other databases:

- EMBL:   U15180
- EMBL:   AL583920
- PIR:   T45206
- RefSeq:   NP_301799.1
- ProteinModelPortal:   P50917
- SMR:   P50917
- EnsemblBacteria:   EBMYCT00000028705
- GeneID:   910180
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1091
- NMPDR:   fig|272631.1.peg.671
- Leproma:   ML1091
- GeneTree:   EBGT00050000018111
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   MLEP272631:ML1091-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34488; Mature: 34356

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00068 MDH; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: ACT_SITE 188-188 BINDING 93-93 BINDING 99-99 BINDING 106-106 BINDING 113-113 BINDING 132-132 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRPLKVAVTGAAGQIGYSLLFRLASGSLLGLDRPIELRLLEIEPALKALEGVVMELDD
CCCCCEEEEEECCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEECHHHHHHHHHHHHHHH
CAFLLLAGVEIGADPNKVFDGVNLALLVGARPRGPGMERGDLLEANGAIFTAQGKALNAV
HHEEEHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCEEEECCCEEEEE
AAADIRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLDHNRAIAQLATKTGSAVTDIR
EEEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHEE
KMTIWGNHSATQYPDVFHAEIGGKNAAEVVGDQAWIEDYFIPTVAKRGAAIIDARGASSA
EEEEECCCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCH
ASAASATIDAARDWLLGTPEGNWVSMAVVSDGSYGVPEGLISSFPVTTTDGDWTIVRGLG
HHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCCCHHHHHCCCCEECCCCEEEEEECC
IDDFSRGRIDKSTAELADERMAVKQLGLI
CCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPRPLKVAVTGAAGQIGYSLLFRLASGSLLGLDRPIELRLLEIEPALKALEGVVMELDD
CCCCEEEEEECCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEECHHHHHHHHHHHHHHH
CAFLLLAGVEIGADPNKVFDGVNLALLVGARPRGPGMERGDLLEANGAIFTAQGKALNAV
HHEEEHCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCEEEECCCEEEEE
AAADIRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLDHNRAIAQLATKTGSAVTDIR
EEEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHHHEE
KMTIWGNHSATQYPDVFHAEIGGKNAAEVVGDQAWIEDYFIPTVAKRGAAIIDARGASSA
EEEEECCCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCCH
ASAASATIDAARDWLLGTPEGNWVSMAVVSDGSYGVPEGLISSFPVTTTDGDWTIVRGLG
HHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCCCCHHHHHCCCCEECCCCEEEEEECC
IDDFSRGRIDKSTAELADERMAVKQLGLI
CCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002