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The map label for this gene is sigE [H]

Identifier: 221230003

GI number: 221230003

Start: 1239380

End: 1240171

Strand: Direct

Name: sigE [H]

Synonym: MLBr_01076

Alternate gene names: 221230003

Gene position: 1239380-1240171 (Clockwise)

Preceding gene: 221230001

Following gene: 221230004

Centisome position: 37.92

GC content: 58.71

Gene sequence:

>792_bases
ATGGGTGACGGTATCGGTATGGAACGTGAAGGGCGCTGGACCGGGAATACACAGTGCCCATTGCGTGTTGTGCCGGGTGA
CGAATCGCCTACCCTCGACGGAAGAGCAAGTCCGGAGGATCTGATTATCACCAACTTGTTGAGCCCGACCATAATGTCTC
ATCCCCCACCCAGCCGAGACGACGACTGGGTAGAACCATTTGACGCGTTGCAGGGCACCGCGGTATTCGACGCGACTGGG
GACAAGGCAACGATGCCGTCGTGGGAGGAATTGGTGCGTCAACACGCGGCTCGGGTATATTGGCTAGCTTATCGCCTTTC
AGGCAACCAGCACGACGCTGAGGACTTGACGCAAGAGACCTTCATTCGAGTGTTCCGATCGGTCCAGAACTACCAGCCAG
GAACCTTTGAGGGATGGCTGCATCGCATCACGACGAATCTGTTCCTTGATATGGTCCGTCGGCGTGCCCGTATCCGCATG
GACGCCTTGCCCGATGATTACGACCGGGTACCTGCCGACGAGCCCAATCCCGAGCAGATCTATCACGATTCGAGGCTGGG
ACCTGATCTACAGGCTGCCTTAGATTCACTGCCGCCGGAATTTCGTGCCGCTGTCGTGCTGTGTGACATCGAAGGTTTGT
CCTACGAGGAGATCGGTGCAACCTTGGGTGTGAAGCTGGGCACCGTACGTAGCCGCATACACCGCGGCCGGCAGGCGCTA
CGCGACTACCTCCTAACCGCGCATCCCAGAGACGGTGCCACCACCGACGCTACGCACGTTAAGTCGGCGTAG

Upstream 100 bases:

>100_bases
TCACATTCGCGGTGTGCACCGCGAATTCTGCTGTGCAGCGCTGTTCAAGCTATGGTTTCTCAGGTAAACCGCAGTTTGCT
CCTATATCGCGTATACGCCG

Downstream 100 bases:

>100_bases
TATCCCCCATTCTCCAAAGACATCGTTCTGTGGTGTCCAGGAGGAACTGGCCGGGCCAAAATAATACTGAGGTTTGCCGG
CGTTATACGAGTATTGGGCA

Product: RNA polymerase sigma factor SigE

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGDGIGMEREGRWTGNTQCPLRVVPGDESPTLDGRASPEDLIITNLLSPTIMSHPPPSRDDDWVEPFDALQGTAVFDATG
DKATMPSWEELVRQHAARVYWLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRM
DALPDDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQAL
RDYLLTAHPRDGATTDATHVKSA

Sequences:

>Translated_263_residues
MGDGIGMEREGRWTGNTQCPLRVVPGDESPTLDGRASPEDLIITNLLSPTIMSHPPPSRDDDWVEPFDALQGTAVFDATG
DKATMPSWEELVRQHAARVYWLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRM
DALPDDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQAL
RDYLLTAHPRDGATTDATHVKSA
>Mature_262_residues
GDGIGMEREGRWTGNTQCPLRVVPGDESPTLDGRASPEDLIITNLLSPTIMSHPPPSRDDDWVEPFDALQGTAVFDATGD
KATMPSWEELVRQHAARVYWLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMD
ALPDDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQALR
DYLLTAHPRDGATTDATHVKSA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=169, Percent_Identity=31.9526627218935, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 29517; Mature: 29386

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDGIGMEREGRWTGNTQCPLRVVPGDESPTLDGRASPEDLIITNLLSPTIMSHPPPSRD
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCC
DDWVEPFDALQGTAVFDATGDKATMPSWEELVRQHAARVYWLAYRLSGNQHDAEDLTQET
CCCCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHH
FIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMDALPDDYDRVPADEPNPEQI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHH
YHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQAL
HCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RDYLLTAHPRDGATTDATHVKSA
HHHHHHCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
GDGIGMEREGRWTGNTQCPLRVVPGDESPTLDGRASPEDLIITNLLSPTIMSHPPPSRD
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCC
DDWVEPFDALQGTAVFDATGDKATMPSWEELVRQHAARVYWLAYRLSGNQHDAEDLTQET
CCCCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHH
FIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRARIRMDALPDDYDRVPADEPNPEQI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHH
YHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIGATLGVKLGTVRSRIHRGRQAL
HCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RDYLLTAHPRDGATTDATHVKSA
HHHHHHCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]