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The map label for this gene is folP2

Identifier: 221229995

GI number: 221229995

Start: 1228816

End: 1229691

Strand: Direct

Name: folP2

Synonym: MLBr_01063

Alternate gene names: 221229995

Gene position: 1228816-1229691 (Clockwise)

Preceding gene: 221229994

Following gene: 221229996

Centisome position: 37.6

GC content: 64.5

Gene sequence:

>876_bases
GTGCAGTCAATGCTGTGCGGCCGGCCGGTCGCAGCGGATCGCCAACTGATCATGGCGATCGTCAACCGTACCCCGGATTC
GTTCTATGACCGGGGCGCGACCTTCAGTGACGAGGCTGCTCGTGCTGCAGCGCACCGGGCCGTTGCGGAGGGCGCCGATG
TCATCGATGTCGGCGGCGTTAAAGCAGGGCCTGGTCAGGGCGTTGACGTAGACACCGAGATCGCCCGGTTGGTTCCGTTC
ATCGAATGGCTACGCAGCGCCTATACAGACCTGCTGATCAGCGTAGACACCTGGCGAGCAGAGGTAGCAAGACTGGCTTG
CACTGCGGGCGCAGACCTGATCAACGACAGCTGGGGTGGAGCCGACCCGGCCATGCATGAGGTCGCCGCTGAGCTTGGCG
CGGGCCTGGTGTGTTCGCACACCGGTGGCGCGCTGCCCCGCACGCGTCCCTTTCGGGTGAGTTACGGTACGACGACCCGC
GGTGTGGTGGACGATGTGATTCGCCAGGTCACTGCAGCTGCCGAGCGCGCGGTCGCGGCCGGGGTAACCCGCGATTCGGT
GTTGGTCGACCCGACCCACGATTTCGGCAAGAACACTTTCCACGGGCTGCTGCTGTTGCGTCATGTAGACGAACTTGTTA
AGACCGGGTGGCCCGTGCTCATGTCGTTGAGCAATAAGGACTTCGTCGGGGAGACTCTGGGCGTGGGTTTGACGGAACGG
CTAGAGGGTACGTTGGCGGCCACTGCGTTGGCGGCGGCAGCTGGCGTCCGCATGTTTCGGGTGCACGAGGTCGTAGCGAC
CAGGCGGGTTCTGGAGATGGTGGCTTCGATCCAGGGGACGCGTCCCCCAACGCGCACGGTGAGGGGACTCGCATGA

Upstream 100 bases:

>100_bases
TGACGTCCCCGCTCACGCCGAGTGTGCGACTGGTCGCACACTCGGCGTGAAGGATGGTCCCGCAACAATGCGGCTGGTCG
TCACCAGGCAATATTGACTG

Downstream 100 bases:

>100_bases
CGGCATCGGAGTTGATCGCCAGCGCGCTAGCGGCCCGGTCCGGAGATGCGTGGCTCGCGGATCGCAGCTGGAGCCGTCCA
GCCTGGACGATCGACGAATT

Product: dihydropteroate synthase

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQSMLCGRPVAADRQLIMAIVNRTPDSFYDRGATFSDEAARAAAHRAVAEGADVIDVGGVKAGPGQGVDVDTEIARLVPF
IEWLRSAYTDLLISVDTWRAEVARLACTAGADLINDSWGGADPAMHEVAAELGAGLVCSHTGGALPRTRPFRVSYGTTTR
GVVDDVIRQVTAAAERAVAAGVTRDSVLVDPTHDFGKNTFHGLLLLRHVDELVKTGWPVLMSLSNKDFVGETLGVGLTER
LEGTLAATALAAAAGVRMFRVHEVVATRRVLEMVASIQGTRPPTRTVRGLA

Sequences:

>Translated_291_residues
MQSMLCGRPVAADRQLIMAIVNRTPDSFYDRGATFSDEAARAAAHRAVAEGADVIDVGGVKAGPGQGVDVDTEIARLVPF
IEWLRSAYTDLLISVDTWRAEVARLACTAGADLINDSWGGADPAMHEVAAELGAGLVCSHTGGALPRTRPFRVSYGTTTR
GVVDDVIRQVTAAAERAVAAGVTRDSVLVDPTHDFGKNTFHGLLLLRHVDELVKTGWPVLMSLSNKDFVGETLGVGLTER
LEGTLAATALAAAAGVRMFRVHEVVATRRVLEMVASIQGTRPPTRTVRGLA
>Mature_291_residues
MQSMLCGRPVAADRQLIMAIVNRTPDSFYDRGATFSDEAARAAAHRAVAEGADVIDVGGVKAGPGQGVDVDTEIARLVPF
IEWLRSAYTDLLISVDTWRAEVARLACTAGADLINDSWGGADPAMHEVAAELGAGLVCSHTGGALPRTRPFRVSYGTTTR
GVVDDVIRQVTAAAERAVAAGVTRDSVLVDPTHDFGKNTFHGLLLLRHVDELVKTGWPVLMSLSNKDFVGETLGVGLTER
LEGTLAATALAAAAGVRMFRVHEVVATRRVLEMVASIQGTRPPTRTVRGLA

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain

Homologues:

Organism=Escherichia coli, GI87082224, Length=270, Percent_Identity=34.4444444444444, Blast_Score=141, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324073, Length=315, Percent_Identity=29.8412698412698, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHPS2_MYCLE (P0C0X2)

Other databases:

- EMBL:   U15180
- EMBL:   AL583920
- PIR:   T45179
- RefSeq:   NP_301781.1
- ProteinModelPortal:   P0C0X2
- SMR:   P0C0X2
- EnsemblBacteria:   EBMYCT00000028481
- GeneID:   910150
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1063
- NMPDR:   fig|272631.1.peg.653
- Leproma:   ML1063
- GeneTree:   EBGT00050000015914
- HOGENOM:   HBG754410
- OMA:   PRTRPHR
- ProtClustDB:   CLSK871906
- BioCyc:   MLEP272631:ML1063-MONOMER
- BRENDA:   2.5.1.15
- DrugBank:   DB00250
- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489
- Gene3D:   G3DSA:3.20.20.20
- PANTHER:   PTHR20941:SF1
- TIGRFAMs:   TIGR01496

Pfam domain/function: PF00809 Pterin_bind; SSF51717 DHP_synth_like

EC number: 2.5.1.15

Molecular weight: Translated: 30852; Mature: 30852

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00792 DHPS_1; PS00793 DHPS_2; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSMLCGRPVAADRQLIMAIVNRTPDSFYDRGATFSDEAARAAAHRAVAEGADVIDVGGV
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
KAGPGQGVDVDTEIARLVPFIEWLRSAYTDLLISVDTWRAEVARLACTAGADLINDSWGG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
ADPAMHEVAAELGAGLVCSHTGGALPRTRPFRVSYGTTTRGVVDDVIRQVTAAAERAVAA
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
GVTRDSVLVDPTHDFGKNTFHGLLLLRHVDELVKTGWPVLMSLSNKDFVGETLGVGLTER
CCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCHHHH
LEGTLAATALAAAAGVRMFRVHEVVATRRVLEMVASIQGTRPPTRTVRGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MQSMLCGRPVAADRQLIMAIVNRTPDSFYDRGATFSDEAARAAAHRAVAEGADVIDVGGV
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCC
KAGPGQGVDVDTEIARLVPFIEWLRSAYTDLLISVDTWRAEVARLACTAGADLINDSWGG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
ADPAMHEVAAELGAGLVCSHTGGALPRTRPFRVSYGTTTRGVVDDVIRQVTAAAERAVAA
CCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
GVTRDSVLVDPTHDFGKNTFHGLLLLRHVDELVKTGWPVLMSLSNKDFVGETLGVGLTER
CCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCHHHH
LEGTLAATALAAAAGVRMFRVHEVVATRRVLEMVASIQGTRPPTRTVRGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002